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Biochemistry & Molecular Biology
Daniela Celeste Profico, Maurizio Gelati, Daniela Ferrari, Giada Sgaravizzi, Claudia Ricciolini, Massimo Projetti Pensi, Gianmarco Muzi, Laura Cajola, Massimiliano Copetti, Emilio Ciusani, Raffaele Pugliese, Fabrizio Gelain, Angelo Luigi Vescovi
Summary: Translating cell therapies into clinical practice requires robust production protocols to optimize and standardize cell manufacture and cryopreservation in compliance with regulations. Two phase I clinical trials were conducted on ALS and SPMS patients using human neural stem cells, produced from brain tissue samples extracted from fetuses. Rigorous quality control measures were implemented to ensure safety, stability, and reproducibility of the cells used in the trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
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Cell Biology
Nancy Mah, Andreas Kurtz, Antonie Fuhr, Stefanie Seltmann, Ying Chen, Nils Bultjer, Johannes Dewender, Ayuen Lual, Rachel Steeg, Sabine C. Mueller
Summary: The European Bank for induced pluripotent Stem Cells (EBiSC) is a non-profit project that aims to provide storage, quality control, and distribution services for human iPSCs for research worldwide. Through collaboration with the human pluripotent stem cell registry (hPSCreg®), EBiSC has successfully standardized the management of cell lines and associated data, providing a model for other iPSC repositories to enhance the FAIRness of iPSC research globally.
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Biochemical Research Methods
Jinglong Zhang, Xu Zhang, Ying Wang, Feng Zeng, Xing-Ming Zhao
Summary: MAT(2) aligns cells in the manifold space using a deep neural network with a contrastive learning strategy, defining cell triplets based on known cell type annotations to produce a more robust consensus manifold and reconstructing batch-effect-free gene expression, which helps to annotate cell types more effectively.
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Multidisciplinary Sciences
Hyun Ji Kim, Matthew J. Rames, Samuel Tassi Yunga, Randall Armstrong, Mayu Morita, Anh T. P. Ngo, Owen J. T. McCarty, Fehmi Civitci, Terry K. Morgan, Thuy T. M. Ngo
Summary: This study examined the impact of differential centrifugation and freeze/thaw on the profiles of extracellular vesicles (EVs) and cell-free messenger RNA (cf-mRNA) in plasma using flow cytometric analysis. The results revealed that the differential centrifugation and post-freeze/thaw processing affected the EV subpopulations and cf-mRNA levels. Freezing plasma containing residual platelets resulted in irreversible ex vivo generation of EV subpopulations and cf-mRNA transcripts.
SCIENTIFIC REPORTS
(2022)
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Economics
Zheng Gangyang, Peng Xianke, Li Xiaozhen, Kang Yexi, Zhao Xiangeng
Summary: Standardization is crucial for China's nuclear industry, playing a role in accelerating safe and innovative development while enhancing core competitiveness. To address new challenges, it is recommended to establish corresponding group standards and improve integration between standard systems and regulatory frameworks.
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Cell & Tissue Engineering
Takeshi Watanabe, Satoshi Yasuda, Shinji Kusakawa, Takuya Kuroda, Mayumi Futamura, Mitsuhide Ogawa, Hidemi Mochizuki, Eri Kikkawa, Hatsue Furukawa, Masato Nagaoka, Yoji Sato
Summary: The MEASURE study aims to standardize the tumorigenicity evaluation of hPSC-derived CTPs, finding that the HEC assay and MACS can improve sensitivity to residual undifferentiated hPSCs. Multisite experiments confirmed the effectiveness of these methods in detecting low levels of undifferentiated cells.
Review
Cell & Tissue Engineering
Davod Pashoutan Sarvar, Hosein Effatpanah, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: The study highlights the important role of MSC-derived extracellular vesicles (MSC-EVs) in hematopoietic regulation and immune response after transplantation. MSC-EVs directly fuse with hematopoietic stem cell membranes, affecting their fate, and regulate the proliferation and differentiation of hematopoietic stem cells through the release of various bioactive factors.
STEM CELL RESEARCH & THERAPY
(2022)
Editorial Material
Multidisciplinary Sciences
Matthew B. Greenblatt, Shawon Debnath
Summary: As aging progresses, skeletal stem cells in mice lose their ability to generate bone-forming cells and instead promote the generation of bone-resorbing cells.
Review
Agriculture, Dairy & Animal Science
Elizandra Cardoso, Maria da Luz Mathias, Rita I. Monarca, Sofia I. Gabriel
Summary: This study reviewed literature on sperm cell counts in assessing sperm shape abnormalities and proposed standardizing counts to 1000 cells per animal for robust statistical results and cost-effectiveness.
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Cell & Tissue Engineering
Adam Joules, Julianne Connors, Rachel Johnson, Elizabeth A. Van Orsow, David H. McKenna, Sarah Nikiforow, Jerome Ritz, Adrian Gee, Allison Hubel
Summary: Cell therapies are a promising treatment option for various diseases, and standards for most aspects of the cell therapy lifecycle have been established, but there are limited standardized protocols regarding the day-of infusion. Variations were identified in facility layout, product thawing location, and cell therapy lab location, which influenced the time from thaw to infusion. Opportunities for standardizing and streamlining the infusion workflow were identified to facilitate adoption of cell therapies at more hospitals.
Review
Biochemistry & Molecular Biology
Jiang Qian, Xue Gao, Ya-Di Wang, Xue-Ling Li, Jun Hu, Jun-Hong Lu
Summary: Stem cells have shown great potential for tissue regeneration and repair due to their unlimited self-renewal and differentiation capabilities. However, the complex interactions between niches and stem cells, along with the limitations of current systems or methods, pose challenges to the multi-faceted applications of stem cells. Emerging evidence suggests that synchrotron infrared microspectroscopy may offer a promising non-invasive technology for stem cell research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Hau Jun Chan, Yanshree, Jaydeep Roy, George Lim Tipoe, Man-Lung Fung, Lee Wei Lim
Summary: Preclinical studies have shown the potential therapeutic effects of stem cell therapy in Alzheimer's disease, but issues like rejection, tumorigenicity, and ethical concerns remain as barriers to its advancement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
News Item
Multidisciplinary Sciences
Smriti Mallapaty
Summary: Research on using induced pluripotent stem cells for disease treatment has received substantial investment.
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Medicine, Research & Experimental
Ziyang Cui, Hope Wei, Colin Goding, Rutao Cui
Summary: Stem cell pools are homogeneous cell populations that possess self-renewal and differentiation potential. While they exhibit limited heterogeneity during homeostasis, their plasticity becomes apparent under stress, leading to changes in phenotype, constitution, metabolism, and function. Manipulating these factors offers better control of stem cell behavior and has the potential to revolutionize regenerative medicine.
Review
Developmental Biology
Jessica D. Manneken, Peter D. Currie
Summary: The cells of the innate immune system, particularly macrophages, play a vital role in tissue repair and stem cell activation. Recent studies have revealed the complex molecular processes involved in these mechanisms and the direct role of immune cells in coordinating these processes.
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Materials Science, Biomaterials
M. El Fray, D. Strzalkowska, C. Mandoli, F. Pagliari, P. Di Nardo, E. Traversa
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Medicine, General & Internal
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INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2016)
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Biochemistry & Molecular Biology
Arianna Mauretti, Annalisa Neri, Olga Kossover, Dror Seliktar, Paolo Di Nardo, Sonia Melino
MACROMOLECULAR BIOSCIENCE
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Cell & Tissue Engineering
Matteo Ciocci, Federico Mochi, Felicia Carotenuto, Emilia Di Giovanni, Paolo Prosposito, Roberto Francini, Fabio De Matteis, Igor Reshetov, Mauro Casalboni, Sonia Melino, Paolo Di Nardo
STEM CELLS AND DEVELOPMENT
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Biochemistry & Molecular Biology
Sonia Melino, Celeste Santone, Paolo Di Nardo, Bibudhendra Sarkar
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Ana G. Freire, Diana S. Nascimento, Giancarlo Forte, Mariana Valente, Tatiana P. Resende, Stefania Pagliari, Claudia Abreu, Isabel Carvalho, Paolo Di Nardo, Perpetua Pinto-do-O
STEM CELLS AND DEVELOPMENT
(2014)
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Cell Biology
Felicia Carotenuto, Laura Teodori, Anna Maria Maccari, Luciano Delbono, Giuseppe Orlando, Paolo Di Nardo
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
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Transplantation
Carlo Gazia, Riccardo Tamburrini, Amish Asthana, Deborah Chaimov, Sean M. Muir, Domenica I. Marino, Luciano Delbono, Valentina Villani, Laura Perin, Paolo Di Nardo, John Robertson, Giuseppe Orlando
CURRENT OPINION IN ORGAN TRANSPLANTATION
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Maria Federica Caso, Felicia Carotenuto, Paolo Di Nardo, Alberto Migliore, Ana Aguilera, Cruz Matilde Lopez, Mariano Venanzi, Francesca Cavalieri, Antonio Rinaldi
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Chemistry, Multidisciplinary
Sara Politi, Felicia Carotenuto, Antonio Rinaldi, Paolo Di Nardo, Vittorio Manzari, Maria Cristina Albertini, Rodolfo Araneo, Seeram Ramakrishna, Laura Teodori
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Chemistry, Multidisciplinary
Francesca Corsi, Felicia Carotenuto, Paolo Di Nardo, Laura Teodori
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Chemistry, Multidisciplinary
Silvia Baiguera, Costantino Del Gaudio, Felicia Carotenuto, Paolo Di Nardo, Laura Teodori
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Biotechnology & Applied Microbiology
Silvia Baiguera, Costantino Del Gaudio, Paolo Di Nardo, Vittorio Manzari, Felicia Carotenuto, Laura Teodori
BIOMED RESEARCH INTERNATIONAL
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Felicia Carotenuto, Dario Coletti, Paolo Di Nardo, Laura Teodori
EUROPEAN JOURNAL OF TRANSLATIONAL MYOLOGY
(2016)
