Article
Cardiac & Cardiovascular Systems
Natalia S. Torres
Summary: This study demonstrates that the skeletal Na channel isoform (Na(v)1.4) is the primary isoform involved in the priming mechanism in rabbit ventricular cells, regulating the efficiency of excitation-contraction coupling by activating reverse-mode NCX. The results suggest that Na(v)1.4 plays a crucial role in modulating the calcium handling properties during EC coupling in cardiomyocytes.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Cell Biology
Rui Zhao, Xianji Liu, Zenghua Qi, Xiaoqiang Yao, Suk Ying Tsang
Summary: The study found that TRPV1 channels regulate the electrophysiological characteristics of ESC-CMs by controlling calcium release from the sarcoplasmic reticulum (SR) and stimulating Na+/Ca2(+) exchanger (NCX) activity. These novel findings reveal an important interplay between TRPV1 and NCX in regulating the physiological functions of ESC-CMs.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Philipp Hegner, Marzena Drzymalski, Alexander Biedermann, Bernadette Memmel, Melanie Durczok, Michael Wester, Bernhard Floerchinger, Zdenek Provaznik, Christof Schmid, York Zausig, Lars S. Maier, Stefan Wagner
Summary: The novel selective NCX-inhibitor SAR296968 inhibits atrial pro-arrhythmic activity and improves diastolic and contractile function in human atrial myocardium, which may have therapeutic implications, especially for treatment of HFpEF.
Article
Multidisciplinary Sciences
Lei Yang, Rong-Chang Li, Bin Xiang, Yi-Chen Li, Li-Peng Wang, Yun-Bo Guo, Jing-Hui Liang, Xiao-Ting Wang, Tingting Hou, Xin Xing, Zeng-Quan Zhou, Haihong Ye, Ren-Qing Feng, Edward G. Lakatta, Zhen Chai, Shi-Qiang Wang
Summary: The contraction of heart cells is regulated by the signaling interaction between LCCs and RyRs, which is influenced by the nanodistance between JPH2 in the SR and CAV3 in the TT. Our study revealed that SRF and myocardin concurrently regulate the expression of JPH2 and CAV3, leading to enhanced efficiency of LCC-RyR signaling and compensation for LCC down-regulation during hibernation, maintaining heart power and avoiding calcium overload.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Jose R. Lopez, Nancy Linares, Jose A. Adams, Alfredo Mijares
Summary: Chagas disease is a leading cause of death and morbidity in Latin America, and this study reveals that chronic elevation of diastolic Ca2+ concentration is associated with the disease. The role of Na+/Ca2+ exchanger (NCX) in this process is also explored, suggesting that inhibitors of NCX may be a potential therapeutic approach for Chagas cardiomyopathy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Monika Seidel, Camille Rabesahala de Meritens, Louisa Johnson, Dimitris Parthimos, Mark Bannister, Nia Lowri Thomas, Esizaze Ozekhome-Mike, Francis Anthony Lai, Spyros Zissimopoulos
Summary: The study characterizes the network of interactions regulating the activity of the RyR2 homotetramer, identifying the importance of a peptide sequence bridging the beta 8 with beta 9 strand in mediating N-terminus self-association. The beta 8-beta 9 loop is crucial for N-terminal intersubunit interaction, while also interacting with the C-terminal channel pore region in a Ca2+-independent manner. Deletion of the beta 8-beta 9 sequence affects RyR2 subunit oligomerization and intracellular Ca2+ mobilization, indicating a role in channel opening.
CARDIOVASCULAR RESEARCH
(2021)
Article
Engineering, Chemical
Yuhei Ogura, Hiroaki Ito, Shukei Sugita, Masanori Nakamura, Yoshihiro Ujihara
Summary: Mammals and birds have faster heart rates, and birds achieve fast Ca2+ concentration changes by increasing the Ca2+ removal capacity in the central part of the cardiomyocytes.
Article
Cardiac & Cardiovascular Systems
Cherrie H. T. Kong, Mark B. Cannell
Summary: Cardiac excitation-contraction coupling relies on Ca2+ release from intracellular stores triggered by L-type Ca2+ channels. Our study found that voltage changes and channel gating stochasticity can lead to variability in Ca2+ spark timing, but the Ca2+ transient wavefronts remain remarkably consistent. Experimental and computational modeling showed that there may be 4 Ca2+ spark initiating complexes per couplon, which decreases spark latency and increases spark probability.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Iyuki Namekata, Ryosuke Odaka, Shunsuke Hamazaki, Hina Nisaka, Shogo Hamaguchi, Hikaru Tanaka
Summary: A method using fluorescence microscopy and specific inhibitors was developed to evaluate the activity of NCX and SERCA in mouse ventricular cardiomyocytes. Different adrenoceptor stimulations and inhibitors exhibited distinct effects on cytoplasmic Ca2+ concentration in non-beating ventricular cardiomyocytes, suggesting potential applications for pharmacological evaluations targeting NCX and SERCA.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Cell Biology
Kateryna Demydenko, Karin R. Sipido, H. Llewelyn Roderick
Summary: The study revealed that InsP3Rs influence Ca2+ handling during ECC by enhancing dyadic Ca2+ fluxes and increasing Ca2+ sparks, which facilitate the activation of RyRs. This mechanism contributes to neurohormonal modulation of cardiac function.
JOURNAL OF CELL SCIENCE
(2021)
Review
Physiology
Pura Bolanos, Juan C. Calderon
Summary: The excitation-contraction coupling in skeletal muscle is a crucial link between membrane excitation and mechanical contraction mediated by Ca2+, involving multiple mechanisms of Ca2+ release and recycling.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biology
Rikke Birkedal, Martin Laasmaa, Jelena Branovets, Marko Vendelin
Summary: The development of the heart involves an increase in structural density and energy transfer efficiency. However, this increased density may restrict overall ATP/ADP diffusion. Disruption of this modular design in failing hearts may compromise intracellular energy transfer.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Review
Physiology
L. A. Blatter, G. Kanaporis, E. Martinez-Hernandez, Y. Oropeza-Almazan, K. Banach
Summary: This passage discusses the process of excitation-contraction coupling in cardiac muscle, comparing the structural and functional attributes of ECC between atrial and ventricular tissue, as well as exploring novel paradigms of atrial ECC and Ca2+ release. Additionally, it delves into the role of the IP3 receptor Ca2+ release channel and its involvement in beat-to-beat ECC, nuclear Ca2+ signaling, and arrhythmogenesis. Finally, it examines electromechanical and Ca2+ alternans and their implications for atrial arrhythmia.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pi-Cheng Cheng, Ruo-Ciao Cheng, Rong-Chi Huang
Summary: Glutamate induces Ca2+ signaling in the suprachiasmatic nucleus (SCN) by activating intracellular mechanisms involving Na+ loads, Na+/K+-ATPase (NKA), and Na+/Ca2+-exchanger (NCX). The presence of Na+ loads leads to slower Ca2+ clearance and increased rebound Ca2+ suppression. However, even in the absence of external Na+, additional Ca2+ handlers are involved in the slower Ca2+ clearance induced by glutamate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Fatemeh Kermani, Matias Mosqueira, Kyra Peters, Enrico D. D. Lemma, Kleopatra Rapti, Dirk Grimm, Martin Bastmeyer, Magdalena Laugsch, Markus Hecker, Nina D. D. Ullrich
Summary: The prospective use of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) for cardiac regenerative medicine strongly depends on the electro-mechanical properties of these cells, especially regarding the Ca2+-dependent excitation-contraction (EC) coupling mechanism. Currently, the immature structural and functional features of hiPSC-CM limit the progression towards clinical applications. Here, we show that a specific microarchitecture is essential for functional maturation of hiPSC-CM.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Article
Materials Science, Biomaterials
Shaohua Wu, Ranjie Xu, Bin Duan, Peng Jiang
JOURNAL OF MATERIALS CHEMISTRY B
(2017)
Editorial Material
Cardiac & Cardiovascular Systems
Ji-Dong Fu, Kenneth R. Laurita
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Emre Bektik, Adrienne Dennis, Gary Pawlowski, Chen Zhou, Danielle Maleski, Satoru Takahashi, Kenneth R. Laurita, Isabelle Deschenes, Ji-Dong Fu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Cell & Tissue Engineering
Ranjie Xu, Andrew T. Brawner, Shenglan Li, Jing-Jing Liu, Hyosung Kim, Haipeng Xue, Zhiping P. Pang, Woo-Yang Kim, Ronald P. Hart, Ying Liu, Peng Jiang
Article
Cell & Tissue Engineering
Hyosung Kim, Ranjie Xu, Ragunathan Padmashri, Anna Dunaevsky, Ying Liu, Cheryl F. Dreyfus, Peng Jiang
Review
Cell Biology
Emre Bektik, Ji-dong Fu
Editorial Material
Cell & Tissue Engineering
Peng Jiang, Luka Turkalj, Ranjie Xu
Article
Virology
Ivan Trus, Nathalie Berube, Peng Jiang, Janusz Rak, Volker Gerdts, Uladzimir Karniychuk
Article
Multidisciplinary Sciences
Ranjie Xu, Xiaoxi Li, Andrew J. Boreland, Anthony Posyton, Kelvin Kwan, Ronald P. Hart, Peng Jiang
NATURE COMMUNICATIONS
(2020)
Article
Cardiac & Cardiovascular Systems
Prasongchai Sattayaprasert, Sunil K. Vasireddi, Emre Bektik, Oju Jeon, Mohammad Hajjiri, Judith A. Mackall, Christine S. Moravec, Eben Alsberg, Jidong Fu, Kenneth R. Laurita
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Dandan Yang, Xiaoping Wan, Adrienne T. Dennis, Emre Bektik, Zhihua Wang, Mauricio G. S. Costa, Charline Fagnen, Catherine Venien-Bryan, Xianyao Xu, Daniel H. Gratz, Thomas J. Hund, Peter J. Mohler, Kenneth R. Laurita, Isabelle Deschenes, Ji-Dong Fu
Summary: This study reveals a novel biophysical action of endogenous miRs in modulating cardiac electrophysiology through noncanonical mechanisms. Endogenous miR1 physically binds with cardiac membrane protein Kir2.1, suppressing I-K1 and affecting action potential of cardiomyocytes. The findings suggest that miRs may be involved in the pathogenesis of cardiac arrhythmias by regulating ion channel physiology and pathology.
Article
Physiology
Yang Zheng, Xiaoping Wan, Dandan Yang, Angelina Ramirez-Navarro, Haiyan Liu, Ji-Dong Fu, Isabelle Deschenes
Summary: Na(v)1.5, a predominant sodium channel in the heart, plays a crucial role in maintaining normal heart rhythm and function. Dysfunctions in Na(v)1.5 are associated with various cardiac pathologies and sudden cardiac death. In heart failure patients, a splice variant Na(v)1.5-G1642X reduces sodium currents through biophysical coupling with the wildtype channel.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Ranjie Xu, Andrew J. Boreland, Xiaoxi Li, Caroline Erickson, Mengmeng Jin, Colm Atkins, Zhiping P. Pang, Brian P. Daniels, Peng Jiang
Summary: By coculturing human pluripotent stem cell (hPSC)-derived microglia and primitive macrophage progenitors, a new brain region-specific organoid model containing microglia has been established. This model allows control of the number of human microglia, which exhibit phagocytic activity and synaptic pruning function. Additionally, human microglia in the organoids respond to Zika virus infection, providing a novel platform to study human microglial function in various neurological disorders.
Article
Cell & Tissue Engineering
Mengmeng Jin, Mahabub Maraj Alam, Alice Y. -C. Liu, Peng Jiang
Summary: Immunodeficient mice are widely used in human stem cell transplantation research. This study found that in Rag2-/- mouse brains, lipofuscin accumulation occurs earlier and spreads more extensively, emitting strong autofluorescence. Surprisingly, in Rag1-/- mouse brains, lipofuscin autofluorescence appears at much older ages compared to Rag2-/- mice. These findings provide important background information for studies using aged immunodeficient mice.
Article
Biochemical Research Methods
Hyosung Kim, Peng Jiang
Summary: The article provides a step-by-step protocol for generating fused forebrain organoids derived from human pluripotent stem cells, which recapitulate human oligodendroglial development. The protocol offers innovative insights into human myelination research in vitro.