Editorial Material
Oncology
Eunmi Lee, Yibin Kang
Summary: The combination of single-cell lineage tracing and RNA sequencing has opened up unprecedented opportunities for prospectively exploring metastatic dynamics. In a pancreatic cancer model, Simeonov et al. developed the macsGESTALT lineage recording system, revealing that hybrid EMT states and S100 expression are associated with increased metastatic abilities.
Article
Anatomy & Morphology
Fatma Kose, Imdat Orhan, Aydin Alan, Ahmet Cabir, Feyzullah Beyaz, Ayhan Duzler
Summary: This study examined the junction of the cornea and sclera in the eyes of various animals using different microscopy techniques. It found that the cornea and sclera fused together and had distinct boundaries in the limbus area. Furthermore, the anatomical characteristics of the limbus differed among species and eye positions.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Review
Oncology
Calum Gabbutt, Nicholas A. Wright, Ann-Marie Baker, Darryl Shibata, Trevor A. Graham
Summary: The dynamical process of cell division that maintains homeostasis in the human body cannot be observed directly. Instead, it is measured through somatic genetic or epigenetic mutations in tissues. Mathematical analysis of somatic clone sizes provides quantitative information on cell birth, death, and replacement rates. This review explores the use of different somatic mutation types for lineage tracing in human tissues, introduces mathematical concepts for inferring dynamic information from clone size data, and discusses the implications of this approach for understanding homeostasis and cancer development.
JOURNAL OF PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Michael Spencer Chapman, Anna Maria Ranzoni, Brynelle Myers, Nicholas Williams, Tim H. H. Coorens, Emily Mitchell, Timothy Butler, Kevin J. Dawson, Yvette Hooks, Luiza Moore, Jyoti Nangalia, Philip S. Robinson, Kenichi Yoshida, Elizabeth Hook, Peter J. Campbell, Ana Cvejic
Summary: By reconstructing a phylogenetic tree of blood development in healthy human fetuses, it was found that individual haematopoietic progenitors acquire multiple somatic mutations by 18 weeks after conception. The data also support a hypoblast origin of the extra-embryonic mesoderm and primitive blood in humans.
Review
Biochemistry & Molecular Biology
Maria Figueres-Onate, Rebeca Sanchez-Gonzalez, Laura Lopez-Mascaraque
Summary: Understanding the strategies used to study cell lineages in the brain and their role in deciphering functional clonal relationships among neural cells; future perspectives will focus on studying cell heterogeneity and the origins of different cell lineages.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Engineering, Biomedical
Hyuna Kim, Anna Wirasaputra, Farnaz Mohammadi, Aritra Nath Kundu, Jennifer A. E. Esteves, Laura M. M. Heiser, Aaron S. S. Meyer, Shelly R. R. Peyton
Summary: Breast cancer is a major cause of cancer-related deaths worldwide, primarily due to metastasis and dormancy. Dormancy, a prolonged period between tumor resection and relapse, poses significant challenges in current therapies. To overcome this, a live cell lineage approach is used to track and distinguish dormant cells from proliferative and dying cells in various in vitro microenvironments. The study reveals that the proportion of long-term quiescent cells is regulated by both intrinsic and extrinsic factors, with the highest percentage found in 3D collagen gels. This approach is expected to benefit the study of dormant tumor cells for biologists and bioengineers in the dormancy community.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell Biology
Yue Ruan, Subao Jiang, Aytan Musayeva, Norbert Pfeiffer, Adrian Gericke
Summary: This article discusses the regulation of corneal epithelium regeneration by the limbal stem cell reservoir, the consequences of limbal stem cell deficiency caused by various factors, and the therapeutic options available for treating the condition.
Article
Multidisciplinary Sciences
Vasiliki Kostiou, Huairen Zhang, Michael W. J. Hall, Philip H. Jones, Benjamin A. Hall
Summary: A study found that using transgenic mouse cell tracking is an effective way to study progenitor cell dynamics, requiring three parameters to describe growth dynamics. By comparing different analysis strategies, an approximate Bayesian computation-based approach was determined to be the most efficient.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lei-Lei Wang, Carolina Serrano, Xiaoling Zhong, Shuaipeng Ma, Yuhua Zou, Chun-Li Zhang
Summary: Recent studies have shown that in vivo cell fate conversions may not actually be converting astrocytes into neurons, but instead utilizing endogenous neurons. Lineage tracing strategies are necessary to accurately track the origin of converted cells, highlighting the importance of applying stringent tracing techniques in studies of cell fate conversions in vivo.
Review
Immunology
Longyun Ye, Saimeng Shi, Wei Chen
Summary: Pancreatic cancer patients do not benefit from immunotherapy like patients with gastrointestinal tumors, potentially due to the presence of innate immune lymphocytes in the tumor microenvironment. Further research on the function and origin of these lymphocytes could provide guidance for immunotherapy in pancreatic cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Urology & Nephrology
Yoshiharu Muto, Benjamin D. Humphreys
Summary: Lineage tracing was originally developed to identify all progeny of a single cell during morphogenesis, but has since been applied to organ homeostasis and injury recovery. Modern techniques rely on reporter gene expression induced by cell-specific DNA recombination, and recent advances include intersectional genetics and sequencing-based genomic lineage tracing.
KIDNEY INTERNATIONAL
(2021)
Review
Cell Biology
Cheng Chen, Yuanxin Liao, Guangdun Peng
Summary: Single-cell lineage tracing, a fundamental goal in modern biology, allows the depiction of cells' history, state, and fate. Various strategies, including integration barcodes, polylox barcodes, and CRISPR barcodes, have been developed to achieve single-cell lineage tracing at high resolution. Despite their successful application in animal development and stem cell research, careful implementation and effort are still required.
Article
Developmental Biology
Travis T. Parsons, Sheila Mosallaei, Laurel A. Raftery
Summary: During Drosophila oogenesis, somatic follicle cells differentiate and secrete components of the eggshell. A timeline of reproducible milestones for centripetal migration, which encloses the oocyte after secretion, is defined using time-lapse imaging of egg chamber explants. Inward migration occurs in two phases, with leading centripetal follicle cells ingressing over the anterior oocyte first and then followed by collective movement of the following cells towards the anterior. E-cadherin is required for normal ingression of the leading cells.
Article
Biochemistry & Molecular Biology
Dian Yang, Matthew G. Jones, Santiago Naranjo, William M. Rideout III, Kyung Hoi (Joseph) Min, Raymond Ho, Wei Wu, Joseph M. Replogle, Jennifer L. Page, Jeffrey J. Quinn, Felix Horns, Xiaojie Qiu, Michael Z. Chen, William A. Freed-Pastor, Christopher S. McGinnis, David M. Patterson, Zev J. Gartner, Eric D. Chow, Trever G. Bivona, Michelle M. Chan, Nir Yosef, Tyler Jacks, Jonathan S. Weissman
Summary: Tumor evolution is driven by progressive genetic and epigenetic alterations, enabling unrestricted growth and expansion. This study provides insights into the hierarchical nature of tumor evolution through tracking phylogenetic relationships between cancer cells, allowing for in-depth studies of tumor progression.
Review
Neurosciences
Jorge Garcia-Marques, Isabel Espinosa-Medina, Tzumin Lee
Summary: Reconstructing the genealogy of cells in an organism is a challenging task in developmental biology, with implications for understanding normal and pathological development. Various strategies have been used for lineage tracing, from direct observation to genetic approaches. The achievements and limitations of past and current technology have been discussed, along with speculation on reaching future milestones in the field.
PROGRESS IN NEUROBIOLOGY
(2021)
Article
Respiratory System
Nikhil T. Awatade, Sharon L. Wong, Alexander Capraro, Elvis Pandzic, Iveta Slapetova, Ling Zhong, Nihan Turgutoglu, Laura K. Fawcett, Renee M. Whan, Adam Jaffe, Shafagh A. Waters
Summary: By comparing the impact of two culture methods on the physiological function of airway cells in vitro, it was found that initial expansion culture conditions significantly influence CFTR activity, which may lead to false conclusions when comparing data from different labs without specific reference ranges.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Article
Oncology
Vanisri Raviraj, Binh T. T. Pham, Byung J. Kim, Nguyen T. H. Pham, Lai F. Kok, Nicole Painter, Naomi C. Delic, Stephen K. Jones, Brian S. Hawkett, J. Guy Lyons
Summary: Superparamagnetic iron oxide nanoparticles were found to enhance the transdermal delivery of anticancer drugs, increasing their penetration and improving the therapeutic effects on skin tumors. This effect was achieved through simple co-administration without the need for conjugation or encapsulation of the drugs.
CANCER NANOTECHNOLOGY
(2021)
Article
Oncology
Fatemeh Zolghadr, Nigel Tse, Dikasya Loka, George Joun, Sreelakshmi Meppat, Victor Wan, Hans Zoellner, Munira Xaymardan, Camile S. Farah, J. Guy Lyons, Eric Hau, Ellis Patrick, Naisana Seyedasli
Summary: This study demonstrates a hybrid epithelial-mesenchymal state in oral squamous cell carcinoma cells and tumour samples, with a key role identified for E-cadherin levels in stratifying cells to compartments with varying levels of radiation response. This response to radiation involves the generation of a new cell population with low E-cadherin expression and Vimentin positivity, showing enhanced capacity for radiation resistance and invasion. Transcription analysis highlights the Wnt signaling pathway's role in generating this cell population, influencing radiation resistance and tumour cell invasion.
BRITISH JOURNAL OF CANCER
(2021)
Article
Cell Biology
Yang Emma Li, Yichang Wang, Ximing Du, Tizhong Zhang, Hoi Yin Mak, Sarah E. Hancock, Holly McEwen, Elvis Pandzic, Renee M. Whan, Yvette Celine Aw, Ivan E. Lukmantara, Yiqiong Yuan, Xiuju Dong, Anthony Don, Nigel Turner, Shiqian Qi, Hongyuan Yang
Summary: TMEM41B and VMP1 are integral membrane proteins of the endoplasmic reticulum, regulating the formation of lipid droplets and lipoproteins, and playing a crucial role in viral infection. They act as phospholipid scramblases, affecting the cellular distribution of cholesterol and phosphatidylserine.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Optics
Kyla Berry, Mike Taormina, Zoe Maltzer, Kristen Turner, Melissa Gorham, Thuc Nguyen, Robert Serafin, Philip R. Nicovich
Summary: This passage discusses the importance of illumination in fluorescence microscopy and the developments in fiber-coupled and speckle reduction technologies. It also introduces a new design, the EvenField Illuminator, available for researchers to integrate into their own imaging systems.
Article
Chemistry, Multidisciplinary
Aria Ahmed-Cox, Elvis Pandzic, Stuart T. Johnston, Celine Heu, John McGhee, Friederike M. Mansfeld, Edmund J. Crampin, Thomas P. Davis, Renee M. Whan, Maria Kavallaris
Summary: Nanoparticles show promise in cancer therapy, but face challenges in clinical trials. Developing advanced 3D cell models like tumor spheroids can help evaluate nanoparticle designs and understand how different cell characteristics influence uptake. A new analysis platform, DONUTS, allows for spatial and temporal quantification of nanoparticle uptake in 3D tumor spheroids, offering insight into penetration kinetics and cell type variations.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Multidisciplinary Sciences
Kendelle J. Murphy, Daniel A. Reed, Claire Vennin, James R. W. Conway, Max Nobis, Julia X. Yin, Cecilia R. Chambers, Brooke A. Pereira, Victoria Lee, Elysse C. Filipe, Michael Trpceski, Shona Ritchie, Morghan C. Lucas, Sean C. Warren, Joanna N. Skhinas, Astrid Magenau, Xanthe L. Metcalf, Janett Stoehr, Gretel Major, Ashleigh Parkin, Romain Bidanel, Ruth J. Lyons, Anaiis Zaratzian, Michael Tayao, Andrew Da Silva, Lea Abdulkhalek, Anthony J. Gill, Amber L. Johns, Andrew Biankin, Jaswinder Samra, Sean M. Grimmond, Angela Chou, Jacky G. Goetz, Michael S. Samuel, J. Guy Lyons, Andrew Burgess, C. Elizabeth Caldon, Lisa G. Horvath, Roger J. Daly, Nikolaj Gadegaard, Yingxiao Wang, Owen J. Sansom, Jennifer P. Morton, Thomas R. Cox, Marina Pajic, David Herrmann, Paul Timpson
Summary: In this study, transient manipulation of focal adhesion kinase in PDAC was visualized using intravital fluorescence imaging, with real-time quantification of cell cycle reporter to improve chemotherapy response. Analysis of different matrices and environmental cues helped to reduce PDAC metastasis.
Article
Cell Biology
Aria Ahmed-Cox, Alexander M. Macmillan, Elvis Pandzic, Renee M. Whan, Maria Kavallaris
Summary: A key challenge in nanomedicine is to understand the systematic delivery of nanoparticles in live cells. This study explores the use of RapidFLIM technology to track and quantify changes in the fluorescence lifetimes of nanoparticles, providing valuable insights in nanoparticle design and translation.
Article
Multidisciplinary Sciences
Sharon L. Wong, Nikhil T. Awatade, Miro A. Astore, Katelin M. Allan, Michael J. Carnell, Iveta Slapetova, Po-chia Chen, Alexander Capraro, Laura K. Fawcett, Renee M. Whan, Renate Griffith, Chee Y. Ooi, Serdar Kuyucak, Adam Jaffe, Shafagh A. Waters
Summary: In this study, the characterization of the I37R mutation in the lasso motif of the CFTR chloride channel was conducted. It was found that the I37R-CFTR mutation results in a residual function defect that can be treated with potentiators and type III correctors. This study provides insights into CFTR channel regulation and offers a potential pathway for drug access in CF patients with ultra-rare genotypes.
Article
Biochemistry & Molecular Biology
Sharon L. Wong, Nikhil T. Awatade, Miro A. Astore, Katelin M. Allan, Michael J. Carnell, Iveta Slapetova, Po-Chia Chen, Jeffry Setiadi, Elvis Pandzic, Laura K. Fawcett, John R. Widger, Renee M. Whan, Renate Griffith, Chee Y. Ooi, Serdar Kuyucak, Adam Jaffe, Shafagh A. Waters
Summary: This study investigated the structural and functional defects of the rare CFTR mutation R352Q in cell models of the airway and gut. The mutation showed residual CFTR function, which could be restored by CFTR potentiators but not the corrector. Molecular dynamics simulations indicated a chloride conductance defect rather than a gating defect. The combination of in vitro patient-derived cell models and in silico simulations can improve predictions of modulator response and aid in CF precision medicine.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Ivan Lukmantara, Fang Chen, Hoi Yin Mak, Armella Zadoorian, Ximing Du, Fanqian Nicole Xiao, Dougall MacMurray Norris, Elvis Pandzic, Renee Whan, Qing Zhong, Hongyuan Yang
Summary: In this study, we identified PI(3)P as a novel regulator of lipid droplet (LD) biogenesis, which impacts LD formation and lipidation through DFCP1. Disruption of cellular PI(3)P homeostasis affects the normal function and localization of DFCP1.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Health Care Sciences & Services
Sharon L. Wong, Elvis Pandzic, Egi Kardia, Katelin M. Allan, Renee M. Whan, Shafagh A. Waters
Summary: Infection control and antibiotic therapy are crucial for managing airway infections in patients with cystic fibrosis. The use of airway epithelial cell cultures grown at air-liquid interface (ALI) provides a physiologically relevant model for studying respiratory infections. Immunofluorescence staining and imaging are effective tools for understanding host-pathogen interactions and exploring potential treatments. A quantitative fluorescence microscopy assay using a graphical user interface (GUI) package is described for identifying specific cell populations and pathogen targets in ALI cultures.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Dermatology
Catherine Zilberg, James Guy Lyons, Ruta Gupta, Diona Lee Damian
Summary: The immune system is crucial in the development and suppression of basal cell carcinoma (BCC). Exposure to ultraviolet radiation (UVR) is the primary cause of BCC, especially in lighter skin types. UVR plays a role in creating an immunosuppressive environment and promoting cancer progression. BCC is more common in immunosuppressed patients and may undergo immune-mediated regression. Analysis of BCCs consistently shows the presence of immune infiltrates and associated proteins.
ANNALS OF DERMATOLOGY
(2023)
Review
Dermatology
Catherine Zilberg, James Guy Lyons, Ruta Gupta, Angela Ferguson, Diona Lee Damian
Summary: Cutaneous squamous cell carcinoma (cSCC) is the most common malignancy in immune-suppressed organ transplant recipients (OTRs), and is highly immunogenic. The tumor immune microenvironment in cSCC from OTRs is altered, providing a favorable environment for tumor growth and survival. Understanding this microenvironment is important for prognosis and treatment decisions.
ANNALS OF DERMATOLOGY
(2023)