Article
Cell Biology
Xiaoli Huang, Hongfeng Guo, Xuejun Cheng, Jinyu Zhang, Wenzheng Qu, Qianyun Ding, Qihang Sun, Qiang Shu, Xuekun Li
Summary: Nicotinamide adenine dinucleotide hydrate (NAD+) plays a crucial role in regulating adult neural stem/progenitor cells (aNSPCs) by reducing proliferation, neuronal differentiation, and inducing apoptosis. NAD+ exposure also inhibits neuronal morphological development. Mechanistically, NAD+ exposure alters transcriptome and decreases the expression of genes related to ATP metabolism and the PI3k-Akt signaling pathway.
Article
Cell Biology
William B. McIntyre, Mehran Karimzadeh, Yasser Riazalhosseini, Mohamad Khazaei, Michael G. Fehlings
Summary: Transplantation of neural stem/progenitor cells (NPCs) is a promising regenerative strategy for neural repair. Precisely controlling culture conditions, such as cell density, is crucial for maintaining stem cells. This study found that higher plating density of hiPSC-NPCs led to better neuronal differentiation ability in vitro, highlighting the importance of controlling culture conditions in NPC transplantation therapies.
Article
Pharmacology & Pharmacy
Xiaojiang Liu, Yiqiu Cui, Jun Li, Cheng Guan, Shu Cai, Jinrong Ding, Jianhong Shen, Yixiang Guan
Summary: PTEN inhibitor bpv(pic) promotes the proliferation and differentiation of NSCs into neurons by regulating the expression and phosphorylation of the Akt/mTOR signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Douglas G. Brownfield, Alex Diaz de Arce, Elisa Ghelfi, Astrid Gillich, Tushar J. Desai, Mark A. Krasnow
Summary: The study reveals that FGF signaling plays a critical role in initiating alveolus development in mouse lung by inducing AT2 fate and a secondary signal for AT1 fate, and continuously maintains the existence of AT2 cells throughout life.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Liang Li, Yue Sun, Alexander E. Davis, Sahil H. Shah, Lobna K. Hamed, Man-Ru Wu, Cheng-Hui Lin, Jun B. Ding, Sui Wang
Summary: Neural progenitor cells lengthen their cell cycle to prepare for differentiation, but it is unclear how they avoid being halted in the cell cycle. Our study shows that N6-methyladenosine (m6A) methylation of cell-cycle-related mRNAs ensures proper cell-cycle progression of late-born retinal progenitor cells. Conditional deletion of Mettl14, required for depositing m6A, delays cell-cycle exit of late-born RPCs, while having no effect on retinal development prior to birth. m6A methylation targets mRNAs involved in elongating the cell cycle for degradation and guarantees proper cell-cycle progression. Additionally, Zfp292 is identified as an m6A target and a potent inhibitor of RPC cell-cycle progression.
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Boyoung Shin, Wen Zhou, Jue Wang, Fan Gao, Ellen V. Rothenberg
Summary: Runx factors regulate lineage specification of hematopoietic cells, including T lymphocytes, by binding to specific genomic regions. In early T cell development, dynamic Runx binding shifts are regulated by Runx dosage and functional partners rather than local chromatin state. Limited Runx protein availability leads to premature Runx occupancy at post-commitment binding sites, accelerating T cell lineage development. This regulation involves the activation of T cell-identity and innate lymphoid cell programs by Runx and other transcription factors. Overall, Runx proteins play a crucial role in directing lineage-fate decisions in various cell types.
Article
Biochemistry & Molecular Biology
Edward J. Calabrese, Vittorio Calabrese, Gaurav Dhawan, Rachna Kapoor, James Giordano
Summary: The paper provides a detailed analysis of hormetic dose responses in neural stem cells (NSCs) and their implications for enhancing cell proliferation, differentiation, and resilience to inflammatory stresses. Various agents, including dietary supplements, pharmaceuticals, endogenous ligands, environmental contaminants, and physical agents, were found to induce hormetic dose responses in NSCs, suggesting potential therapeutic applications for neurological injury and disease.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemical Research Methods
Jennifer Hammelman, Tulsi Patel, Michael Closser, Hynek Wichterle, David Gifford
Summary: This study compares nine computational methods for identifying reprogramming factors for cell differentiation. The researchers found that methods using chromatin accessibility performed the best, with an average identification rate of 50-60% of reprogramming factors in the top ten candidates. Among the chromatin accessibility methods, DeepAccess and diffTF showed the highest correlation with the ranked significance of transcription factor candidates. AME and diffTF were identified as optimal methods for transcription factor recovery, allowing for systematic prioritization of candidates in the design of new reprogramming protocols.
Article
Multidisciplinary Sciences
Panetha Pansri, Phetcharat Phanthong, Nopparat Suthprasertporn, Yindee Kitiyanant, Alisa Tubsuwan, Andras Dinnyes, Julianna Kobolak, Narisorn Kitiyanant
Summary: The study found that BDNF can promote cell proliferation of AD-derived hNPCs, but does not have a promoting effect on neurite outgrowth. The JAK2 inhibitor AG490 partially decreased BDNF-induced cell proliferation.
Review
Biochemistry & Molecular Biology
Louise C. Abbott, Fikru Nigussie
Summary: The mammalian brain is formed from billions of cells, with neural progenitor cells giving rise to most of these cells through processes like proliferation, differentiation, and migration. Developing neurons are especially vulnerable to neurotoxicants such as mercury, and this review focuses on how mercury, particularly methylmercury, affects neurogenesis in the developing mammalian brain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Juan Gao, Shuaibing Hou, Shengnan Yuan, Yuxia Wang, Yanan Gao, Xiaolu Sun, Weili Wang, Yajing Chu, Yuan Zhou, Xiaoming Feng, Hongbo R. Luo, Tao Cheng, Jun Shi, Weiping Yuan, Xiaomin Wang
Summary: Research shows that Rheb1-deficiency in neutrophils can remodel the bone marrow environment and induce HSC expansion, possibly by impairing their ability to secrete IL-6, affecting the production of SCF by MSCs, and promoting HSC proliferation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Aaron I. Weiner, Gan Zhao, Hanna M. Zayas, Nicolas P. Holcomb, Stephanie Adams-Tzivelekidis, Joanna Wong, Maria E. Gentile, Dyuthi Reddy, Joey Wei, Gargi Palashikar, Kwaku K. Quansah, Andrew E. Vaughan
Summary: The lung has a robust regenerative response to severe injuries, such as influenza infection, involving the proliferation and differentiation of lung epithelial cells and basal cells. The transcription factor ANp63 plays a critical role in regulating these reparative pathways, and loss of ANp63 leads to increased plasticity of basal cells and dysplastic tissue remodeling.
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Article
Cell & Tissue Engineering
Kee-Pyo Kim, Cui Li, Daria Bunina, Hyun-Woo Jeong, Julia Ghelman, Juyong Yoon, Borami Shin, Hongryeol Park, Dong Wook Han, Judith B. Zaugg, Johnny Kim, Tanja Kuhlmann, Ralf H. Adams, Kyung-Min Noh, Steven A. Goldman, Hans R. Schoeler
Summary: By transducing an optimized transcription factor combination into a permissive donor phenotype, pericyte-derived iOPCs (PC-iOPCs) can overcome the limitations of inefficient generation and limited expansion and differentiation competence. PC-iOPCs are stably expandable and functionally myelinogenic with high differentiation competence.
Article
Biochemistry & Molecular Biology
Loic Broix, Laure Asselin, Carla G. Silva, Ekaterina L. Ivanova, Peggy Tilly, Johan G. Gilet, Nicolas Lebrun, Helene Jagline, Giuseppe Muraca, Yoann Saillour, Nathalie Drouot, Madeline Louise Reilly, Fiona Francis, Alexandre Benmerah, Nadia Bahi-Buisson, Richard Belvindrah, Laurent Nguyen, Juliette D. Godin, Jamel Chelly, Maria-Victoria Hinckelmann
HUMAN MOLECULAR GENETICS
(2018)
Article
Neurosciences
Ivan Gladwyn-Ng, Lluis Cordon-Barris, Christian Alfano, Catherine Creppe, Therese Couderc, Giovanni Morelli, Nicolas Thelen, Michelle America, Bettina Bessieres, Ferechte Encha-Razavi, Maryse Bonniere, Ikuo K. Suzuki, Marie Flamand, Pierre Vanderhaeghen, Marc Thiry, Marc Lecuit, Laurent Nguyen
NATURE NEUROSCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Nicolas Caron, Emmanuelle C. Genin, Quentin Marlier, Sebastien Verteneuil, Pierre Beukelaers, Laurence Morel, Miaofen G. Hu, Philip W. Hinds, Laurent Nguyen, Renaud Vandenbosch, Brigitte Malgrange
CELLULAR AND MOLECULAR LIFE SCIENCES
(2018)
Review
Biochemistry & Molecular Biology
Amandine Czajkowski, Anais Mounier, Laurence Delacroix, Brigitte Malgrange
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Article
Clinical Neurology
Emmanuelle C. Genin, Blandine Madji Hounoum, Sylvie Bannwarth, Konstantina Fragaki, Sandra Lacas-Gervais, Alessandra Mauri-Crouzet, Francoise Lespinasse, Julien Neveu, Baptiste Ropert, Gaelle Auge, Charlotte Cochaud, Cynthia Lefebvre-Omar, Stephanie Bigou, Aude Chiot, Fanny Mochel, Severine Boillee, Christian S. Lobsiger, Delphine Bohl, Jean-Ehrland Ricci, Veronique Paquis-Flucklinger
ACTA NEUROPATHOLOGICA
(2019)
Review
Biochemistry & Molecular Biology
Quentin Marlier, Tine D'aes, Sebastien Verteneuil, Renaud Vandenbosch, Brigitte Malgrange
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Clinical Neurology
Emmanuelle C. Genin, Sylvie Bannwarth, Baptiste Ropert, Francoise Lespinasse, Alessandra Mauri-Crouzet, Gaelle Auge, Konstantina Fragaki, Charlotte Cochaud, Erminia Donnarumma, Sandra Lacas-Gervais, Timothy Wai, Veronique Paquis-Flucklinger
Summary: Genin et al. show that SLP2 and prohibitins play a role in the pathological cascade of ALS caused by the p.Ser59Leu variant of CHCHD10. Aggregates of SLP2/prohibitins and instability of the PHB complex result in mitochondrial fragmentation and cell death.
Article
Neurosciences
Valerie Dion, Nathalie Schumacher, Nathalie Masar, Alexandra Pieltain, Pierre Tocquin, Pierre Lesoinne, Brigitte Malgrange, Renaud Vandenbosch, Rachelle Franzen
Summary: This study investigated the role of Cyclin-dependent kinase 7 (Cdk7) in oligodendrocyte differentiation. The results showed that the loss of Cdk7 led to a decrease in axonal diameter, thinner myelin sheaths, and reduced levels of major myelin-associated proteins during myelination. These findings reveal novel functions for Cdk7 in central nervous system myelination.
Article
Audiology & Speech-Language Pathology
Ronald Pouyo, Keshi Chung, Laurence Delacroix, Brigitte Malgrange
Summary: Post-translational modifications, such as protein ubiquitination, play essential roles in the development and function of the inner ear. Impairment of the ubiquitin-proteasome system (UPS) has been linked to altered cochlear development and hearing loss. This review provides insights into the mechanisms of UPS and its confirmed role in normal hearing and deafness, as well as potential therapeutic strategies for UPS-associated hearing loss.
Article
Biochemistry & Molecular Biology
Tine D'aes, Quentin Marlier, Sebastien Verteneuil, Pascale Quatresooz, Renaud Vandenbosch, Brigitte Malgrange
Summary: This study investigated the role of Cdk7 in ischemic neuronal death using the OGD model. The results showed that invalidation of Cdk7 did not provide neuroprotection and no evidence of apoptosis was found in the OGD model. Neurons exposed to OGD seemed predisposed to die in an NMDA receptor-dependent manner. Due to uncertainties about cell death after OGD, caution is warranted when using this model for identifying new stroke therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Ilyas Chohra, Keshi Chung, Subhajit Giri, Brigitte Malgrange
Summary: During transcription, DNA replication and repair, ATP-dependent chromatin remodeling complexes modify chromatin structure through repositioning and rearranging nucleosomes using the energy of ATP hydrolysis. These complexes are crucial in regulating development and stem cell biology in various organs, including the inner ear, and mutations in genes coding for their protein components have been implicated in neurosensory deafness. This review discusses the composition, structure, and functional activity of ATP-dependent chromatin remodeling complexes and their contribution to hearing and neurosensory deafness.
Article
Biochemistry & Molecular Biology
Ilyas Chohra, Subhajit Giri, Brigitte Malgrange
Summary: Researchers have generated a homozygous CHD4G1003D hESC line (WAe025-A-1) using CRISPR/eCas9-based gene editing in the WA-25 hESC line. The edited hESC line maintains a normal karyotype, pluripotency, and the ability to differentiate into three germ layers. This cell line will serve as a valuable resource for studying the functional role of CHD4 in the development and disease modeling of SIHIWES.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Genetics & Heredity
Emmanuelle C. Genin, Melanie Abou-Ali, Veronique Paquis-Flucklinger
Summary: Mitochondrial dysfunction plays a significant role in amyotrophic lateral sclerosis (ALS) and is closely related to the pathogenesis of the disease. Fighting against mitochondrial dysfunction appears to be a promising avenue for developing combined therapies in the future.
Article
Cell Biology
Mona Veithen, Aurelia Huyghe, Priscilla van den Ackerveken, So-ichiro Fukada, Hiroki Kokubo, Ingrid Breuskin, Laurent Nguyen, Laurence Delacroix, Brigitte Malgrange
Summary: The transcription factor Sox9 plays a crucial role in cell fate determination in the cochlea, with its expression becoming restricted to supporting cells during hair cell differentiation. Sox9 inhibits the transcriptional activity of Atoh1, a key factor for hair cell formation, by upregulating Hey1 and HeyL antagonists. Suppression of Sox9 in sensory progenitors leads to a modest increase in hair cell number, but this is not sufficient to induce supernumerary hair cell production in vivo.
Article
Multidisciplinary Sciences
Jean Defourny, Christiane Peuckert, Klas Kullander, Brigitte Malgrange
