Article
Biochemistry & Molecular Biology
Rucong Liu, Zibaguli Wubulikasimu, Runze Cai, Fanyi Meng, Qinghua Cui, Yuan Zhou, Yang Li
Summary: NAT10-catalyzed N-4-acetylcytidine (ac(4)C) plays an important role in post-transcriptional regulation of mRNA stability. This study shows that NAT10 expression is correlated with pluripotency and that ac(4)C modification preferentially targets core pluripotency transcription factors in hESCs. Inactivation of NAT10 leads to decreased ac(4)C levels, decay of core pluripotency regulator OCT4 transcript, and impairment of self-renewal and early differentiation in hESCs.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Jingsheng Li, Chunhong Dai, Wenyan Xie, Heyao Zhang, Xin Huang, Constantinos Chronis, Ying Ye, Wensheng Zhang
Summary: This study developed a one-step strategy to efficiently target and suppress endogenous pluripotent genes in mouse ESCs and replace their expression with AID-fused transgenes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Jack-Christophe Cossec, Tatiana Traboulsi, Sebastien Sart, Yann Loe-Mie, Manuel Guthmann, Ivo A. Hendriks, Ilan Theurillat, Michael L. Nielsen, Maria-Elena Torres-Padilla, Charles N. Baroud, Anne Dejean
Summary: Recent advances in synthetic embryology have revealed that chemical inhibition of SUMOylation in mouse embryonic stem cells (ESCs) can lead to the generation of embryo-like structures. These structures can self-organize into gastrulating structures and undergo axial organization reminiscent of natural embryo morphogenesis. This approach provides a proof of principle for exploring early embryogenesis by targeting chromatin roadblocks of cell fate change.
Article
Multidisciplinary Sciences
Masaki Kinoshita, Meng Amy Li, Michael Barber, William Mansfield, Sabine Dietmann, Austin Smith
Summary: Genome remethylation is crucial for mammalian development, with a study showing that deficiencies in Dnmt3a and Dnmt3b lead to ES cells developing into trophoblast cells due to failed suppression of Ascl2. Regulating the expression of Ascl2 can impact the transdifferentiation and contribution of ES cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Veronika V. Ermakova, Nikita P. Fokin, Nikolay D. Aksenov, Evgeny I. Bakhmet, Ekaterina V. Aleksandrova, Andrey A. Kuzmin, Alexey N. Tomilin
Summary: The transcription factor Oct4 plays a critical role in maintaining pluripotency and can reprogram somatic cells into a pluripotent state. Identifying the genetic elements that regulate the Oct4-encoding gene, Pou5f1, is important for understanding pluripotency regulation. This study shows that translocating a fragment of the Pou5f1 gene impairs the self-renewal and differentiation of mouse embryonic stem cells, indicating the presence of critical regulatory elements outside the previously determined boundaries of Pou5f1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mikhail L. Vorobev, Bashar A. Alhasan, Irina I. Suvorova
Summary: Autophagy plays a critical role in early embryonic development, particularly in blastocyst formation. The absence of p53 does not affect autophagy activation in pluripotent cells but hinders differentiation induced by retinoic acid. Despite p53 deletion, Ulk1-dependent autophagy is still activated in response to serum deprivation in mESCs, but impaired during differentiation induced by retinoic acid.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Xue Wang, Changyun Ping, Puwen Tan, Chenguang Sun, Guang Liu, Tao Liu, Shuchun Yang, Yanmin Si, Lijun Zhao, Yongfei Hu, Yuyan Jia, Xiaoshuang Wang, Meili Zhang, Fang Wang, Dong Wang, Jia Yu, Yanni Ma, Yue Huang
Summary: The study reveals the critical role of RNA-binding proteins in pluripotency exit of embryonic stem cells, with hnRNPLL being identified as a key regulator of alternative splicing in ES cells. Its depletion leads to sustained expression of ES cell-preferred isoforms, resulting in differentiation deficiency and developmental defects. This highlights the potential application of targeting RBPs in controlling ES cell fate.
Article
Chemistry, Multidisciplinary
Yingjie Hang, Xiaoliang Ma, Chunxiao Liu, Siyuan Li, Sixuan Zhang, Ruyan Feng, Qianwen Shang, Qi Liu, Zhaozhao Ding, Xiaoyi Zhang, Liyin Yu, Qiang Lu, Changshun Shao, Hong Chen, Yufang Shi, Jiuyang He, David L. Kaplan
Summary: The development of anisotropic silk protein nanofiber-based hydrogels enables mouse embryonic stem cells to maintain stemness in vitro without the need for leukemia inhibitory factor and mouse embryonic fibroblasts, resulting in superior pluripotency and genetic stability. The hydrogels establish an improved dynamic niche that stimulates autocrine factor secretion and maintains pluripotency and propagation of ESCs, offering a simple and cost-effective option for expansion and utility of ESCs in research and therapy, while providing insights into early mammalian embryogenesis.
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Ying Ye, Xi Chen, Wensheng Zhang
Summary: Embryonic stem cells have a unique ability to maintain and regulate the balance between self-renewal and multi-lineage cellular differentiation, largely dependent on gene expression regulations. Chromatin remodeling complexes play a crucial role in promoting chromatin structural changes that ultimately affect ESC fate choices.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
David C. Klein, Santana M. Lardo, Kurtis N. McCannell, Sarah J. Hainer
Summary: The FACT complex maintains cellular pluripotency by regulating nucleosome positioning and transcription activity. FACT interacts with genes such as OCT4, SOX2, and NANOG, binding to both promoter and enhancer elements, and regulates their transcription and expression, thereby influencing cell fate decisions.
Article
Engineering, Biomedical
Xiayi Xu, Qian Feng, Xun Ma, Yingrui Deng, Kunyu Zhang, Hon Son Ooi, Boguang Yang, Zhi-Yong Zhang, Bo Feng, Liming Bian
Summary: In this study, a gelatin-based structurally dynamic hydrogel was developed to emulate the intrinsic properties of the extracellular matrix. The dynamic hydrogel significantly promoted the growth and pluripotency maintenance of embryonic stem cells (ESCs).
Article
Cell Biology
Anderson Tan, Renuka Prasad, Eek-hoon Jho
Summary: TFEB plays a crucial role in mESCs by regulating PTN to maintain pluripotency, with interactions involving Nanog and Sox2. While high levels of TFEB are expected to enhance autophagy-lysosomal activity, undifferentiated mESCs surprisingly display low basal levels.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Daniel Olivieri, Eleonora Castelli, Yumiko K. Kawamura, Panagiotis Papasaikas, Ilya Lukonin, Melanie Rittirsch, Daniel Hess, Sebastien A. Smallwood, Michael B. Stadler, Antoine H. F. M. Peters, Joerg Betschinger
Summary: Mouse embryonic stem cells are biased towards producing embryonic endoderm rather than extraembryonic endoderm, which is regulated by transcriptional repressors Hdac3 and Dax1. Pluripotency factors Nr5a2 and Esrrb promote cell type conversion, and perturbation of the barrier extends mESC potency. This study shows that transcriptional repressors stabilize pluripotency by biasing the equilibrium between embryonic and extraembryonic lineages in mESCs.
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Oncology
Osamu Shimomura, Tatsuya Oda, Hiroaki Tateno, Yusuke Ozawa, Sota Kimura, Shingo Sakashita, Masayuki Noguchi, Jun Hirabayashi, Makoto Asashima, Nobuhiro Ohkohchi
MOLECULAR CANCER THERAPEUTICS
(2018)
Article
Cell Biology
Tadayoshi Hayata, Masahiko Chiga, Yoichi Ezura, Makoto Asashima, Hidetaka Katabuchi, Ryuichi Nishinakamura, Masaki Noda
Article
Biochemistry & Molecular Biology
Jacek Stepniewski, Tomasz Pacholczak, Aniela Skrzypczyk, Maciej Ciesla, Agata Szade, Krzysztof Szade, Romain Bidanel, Agnieszka Langrzyk, Radoslaw Grochowski, Felix Vandermeeren, Neli Kachamakova-Trojanowska, Mateusz Jez, Grazyna Drabik, Mahito Nakanishi, Alicja Jozkowicz, Jozef Dulak
Article
Biochemistry & Molecular Biology
Ken-ichi Miyazono, Saho Moriwaki, Tomoko Ito, Akira Kurisaki, Makoto Asashima, Masaru Tanokura
Article
Cell & Tissue Engineering
Akiko Saito, Akio Ooki, Takashi Nakamura, Shoko Onodera, Kamichika Hayashi, Daigo Hasegawa, Takahito Okudaira, Katsuhito Watanabe, Hiroshi Kato, Takeshi Onda, Akira Watanabe, Kenjiro Kosaki, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Teruo Sakamoto, Akira Yamaguchi, Kenji Sueishi, Toshifumi Azuma
STEM CELL RESEARCH & THERAPY
(2018)
Article
Biochemistry & Molecular Biology
Ken-ichi Miyazono, Saho Moriwaki, Tomoko Ito, Akira Kurisaki, Makoto Asashima, Masaru Tanokura
Article
Multidisciplinary Sciences
Keisho Hirota, Mayumi Furuya, Naomi Morozumi, Kazunori Yoshikiyo, Takafumi Yotsumoto, Toshimasa Jindo, Ryuichi Nakamura, Koichiro Murakami, Yohei Ueda, Takeshi Hanada, Hiroko Sade, Sayaka Yoshida, Kei Enomoto, Yugo Kenai, Ichiro Yamauchi, Takafumi Yamashita, Yoriko Ueda-Sakane, Toshihito Fujii, Akihiro Yasoda, Nobuya Inagaki
Article
Multidisciplinary Sciences
Tetsuo Moriguchi, Shuji Takeda, Shinzo Iwashita, Kei Enomoto, Tatsuya Sawamura, Uichi Koshimizu, Toru Kondo
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Naohiro Sunamura, Shinzo Iwashita, Kei Enomoto, Taisuke Kadoshima, Fujio Isono
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Ken-ichi Miyazono, Yosuke Ohno, Hikaru Wada, Tomoko Ito, Yui Fukatsu, Akira Kurisaki, Makoto Asashima, Masaru Tanokura
NUCLEIC ACIDS RESEARCH
(2018)
Article
Pharmacology & Pharmacy
Satoshi Muneoka, Megumi Goto, Tomonari Nishimura, Kei Enomoto, Kumiko Kadoshima-Yamaoka, Yoshiaki Tomimori
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2019)
Article
Medical Ethics
Rei Nouchi, Hiroaki Aihara, Fumie Arie, Makoto Asashima, Hiroyuki Daida, Jun Fudano, Yasuhiro Fujiwara, Shinji Fushiki, Robert J. Geller, Kazuo Hatano, Toshio Homma, Minoru Kimura, Toshio Kuroki, Koichi Miki, Ikuo Morita, Kosaku Nitta, Akira Shinohara, Mikiko C. Siomi, Masayuki Yoshida, Iekuni Ichikawa
ACCOUNTABILITY IN RESEARCH-POLICIES AND QUALITY ASSURANCE
(2020)
Article
Multidisciplinary Sciences
Yumeko Satou-Kobayashi, Jun-Dal Kim, Akiyoshi Fukamizu, Makoto Asashima
Summary: Activin, a member of the TGF-beta superfamily, induces various tissues in amphibian presumptive ectoderm in vitro, but it is unclear how closely the resulting cells reflect in vivo development. Transcriptome analysis of Xenopus ACs treated with activin A showed increasing number of differentially expressed genes over time, reflecting the molecular characteristics of early normal embryogenesis.
SCIENTIFIC REPORTS
(2021)
Meeting Abstract
Endocrinology & Metabolism
Tadayoshi Hayata, Yoichi Ezura, Makoto Asashima, Ryuichi Nishinakamura, Masaki Noda
JOURNAL OF BONE AND MINERAL RESEARCH
(2019)
Meeting Abstract
Oncology
Tatsuya Oda, Osamu Shimomura, Hiroaki Tateno, Jun Hirabayashi, Masayuki Noguchi, Shigeru Chiba, Makoto Asashima, Nobuhiro Ohkohchi