Article
Multidisciplinary Sciences
Helene Bjoerg Kristensen, Thomas Levin Andersen, Andrea Patriarca, Klaus Kallenbach, Birgit MacDonald, Tanja Sikjaer, Charlotte Ejersted, Jean-Marie Delaisse
Summary: The dormancy and formation of hematopoietic stem cells and progenitors are guided by signals from the bone marrow microenvironment. Research on the microenvironment of murine hematopoietic stem cells is more comprehensive, while knowledge on murine progenitor microenvironments and even more so in humans is lacking. Characterizing these microenvironments is crucial for understanding hematopoiesis and developing treatments for bone marrow malignancies, but challenging due to difficulties in detecting hematopoietic stem cells in the complex bone marrow landscape.
Article
Cell Biology
Maria Florencia Tellechea, Flavia S. Donaires, Vinicius S. de Carvalho, Barbara A. Santana, Fernanda B. da Silva, Raissa S. Tristao, Lilian F. Moreira, Aline F. de Souza, Yordanka M. Armenteros, Lygia Pereira, Rodrigo T. Calado
Summary: This study used iPSCs to model immune aplastic anemia and found that the differentiation potential of hematopoietic cells in this disease is significantly reduced, suggesting that specific epigenetic changes in HSPCs caused by immune attack may limit cell proliferation and differentiation.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Hongchen Liu, Xiaoli Zheng, Chengtao Zhang, Jiajun Xie, Beibei Gao, Jing Shao, Yan Yang, Hengxiang Wang, Jinsong Yan
Summary: The study demonstrated the efficacy of haploidentical bone marrow transplantation (haplo-BMT) for patients with severe aplastic anemia II (SAA-II), achieving a 3-year overall survival and disease-free survival rate of 86.4% +/- 0.73%. This indicates that haplo-BMT could be considered as an alternative treatment strategy for SAA-II patients lacking HLA-matched donors.
FRONTIERS OF MEDICINE
(2021)
Article
Immunology
Rene Reitermaier, Thomas Krausgruber, Nikolaus Fortelny, Tanya Ayub, Pablo Augusto Vieyra-Garcia, Philip Kienzl, Peter Wolf, Anke Scharrer, Christian Fiala, Marita Kolz, Manuela Hiess, Martin Vierhapper, Christopher Schuster, Andreas Spittler, Christof Worda, Wolfgang Weninger, Christoph Bock, Rene Reitermaier, Adelheid Elbe-Buerger
Summary: Single-cell analyses identified a naive T cell population expressing specific T cell receptors enriched in fetal skin and intestine. These cells may contribute to early skin development and fetal immune defense, showing fundamental differences in immune surveillance between fetal and adult human skin.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biology
A. Lorzadeh, C. Hammond, F. Wang, D. J. H. F. Knapp, J. Ch Wong, J. Y. A. Zhu, Q. Cao, A. Heravi-Moussavi, A. Carles, M. Wong, Z. Sharafian, J. Steif, M. Moksa, M. Bilenky, P. M. Lavoie, C. J. Eaves, M. Hirst
Summary: By generating comprehensive reference epigenomes of normal human cord blood, we have identified differences in chromatin modifications between different cell types. Particularly, the repressive H3K27me3 mark is retained in lymphoid cells but lost in monocytes and erythroblasts. Inhibition of polycomb group members also affects the fate decisions of neonatal hematopoietic progenitor cells.
Article
Medicine, Research & Experimental
Hui Li, Dexiao Kong, Yi Zhao, Xia Liu, Fang Xiao, Xiaoyan Li, Jianting Hu, Yingjie Chen, Shengli Li, Baozhu Wang, Yuan Chen, Yang Jiang, Xiaoli Liu, Xiumei Feng, Yanan Guo, Xiaoli Feng, Jing Ren, Fang Wang, Ying Han, William Donelan, Lijun Yang, Dawei Xu, Dongqi Tang, Chengyun Zheng
Summary: This study investigates the therapeutic effect of irisin in an AA mouse model. The results show that irisin plays a role in the pathogenesis of AA and can protect HPSC through stimulation of proliferation and regulation of mitochondria function, providing evidence for the application of irisin in AA therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Leonid Olender, Roshina Thapa, Roi Gazit
Summary: In this study, the researchers propose using CD34/CD150 instead of the traditional CD34/Fc gamma R approach to separate sub-populations in the LK compartment. They re-analyzed single-cell RNA-Seq data and confirmed their findings through independent FACS analysis. The newly-obtained LK sub-populations showed comparable differentiation potential to the classical ones.
Review
Biochemistry & Molecular Biology
Antonio G. Solimando, Carmen Palumbo, Mary Victoria Pragnell, Max Bittrich, Antonella Argentiero, Markus Krebs
Summary: In recent years, it has become evident that bone marrow failures and myeloid malignancy predisposition syndromes have a wide range of phenotypes. Diagnosis of these diseases should be considered in patients with unexplained hematopoiesis defects. Treatment of hypocellular bone marrow failure remains challenging. Exogenous stressors, immune defects, and constitutional genetic defects contribute to disease progression. Understanding the diverse phenotypes of inherited and acquired bone marrow failures and predispositions to myeloid malignancies is crucial. Exploring the pathomechanisms of bone marrow failure may lead to the discovery of new therapeutic and diagnostic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Harry Lesmana, Timothy Jacobs, Michelle Boals, Nathan Gray, Sara Lewis, Juan Ding, Guolian Kang, Melvanique Hale, Mitchell Weiss, Ulrike Reiss, Winfred Wang, Marcin Wlodarski
Summary: This study evaluated the efficacy and safety of standard immunosuppressive therapy and combination therapy with eltrombopag in pediatric severe aplastic anemia patients. The results showed a higher objective response rate in the treatment group with eltrombopag added, and no significant differences in infections between the two therapies.
PEDIATRIC BLOOD & CANCER
(2021)
Article
Cell Biology
Jyotika Srivastava, Shobhita Katiyar, Chandra P. Chaturvedi, Soniya Nityanand
Summary: Extracellular vesicles released by mesenchymal stromal cells from severe aplastic anemia patients impair the function of hematopoietic stem and progenitor cells.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Meng Wang, Laura T. L. Brandt, Xiaonan Wang, Holly Russell, Emily Mitchell, Ashley N. Kamimae-Lanning, Jill M. Brown, Felix A. Dingler, Juan I. Garaycoechea, Tomoya Isobe, Sarah J. Kinston, Muxin Gu, George S. Vassiliou, Nicola K. Wilson, Berthold Gottgens, Ketan J. Patel
Summary: The study reveals that metabolism-derived formaldehyde-DNA damage stimulates the p53 response in HSCs, leading to accelerated aging.
Review
Biochemistry & Molecular Biology
Xing-An Wang, Ju-Pi Li, Kang-Hsi Wu, Shun-Fa Yang, Yu-Hua Chao
Summary: Aplastic anemia is a rare but life-threatening disease characterized by low blood cell count and reduced cellularity in the bone marrow. Mesenchymal stem cell dysfunction is believed to play a role in the development of the disease. This review provides a comprehensive understanding of the involvement of mesenchymal stem cells in acquired idiopathic aplastic anemia and discusses their clinical application. The pathophysiology of the disease, properties of mesenchymal stem cells, and results of mesenchymal stem cell therapy in animal models are also described. The authors anticipate that more patients with aplastic anemia can benefit from mesenchymal stem cell therapy in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Xiaodi Ma, Yangyang Zuo, Zhengli Xu, Yuanyuan Zhang, Yifei Cheng, Tingting Han, Pan Suo, Yuqian Sun, Feifei Tang, Fengrong Wang, Chenhua Yan, Yuhong Chen, Yu Wang, Xiaohui Zhang, Kaiyan Liu, Xiaojun Huang, Lanping Xu
Summary: This study retrospectively compared the characteristics and outcomes of patients with hepatitis-associated aplastic anemia (HAAA) and non-hepatitis-associated aplastic anemia (non-HAAA) who underwent haploidentical hematopoietic stem cell transplantation (haplo-HSCT). The results showed no significant differences in neutrophil engraftment time, overall survival rates, and liver event-free survival rates between HAAA and non-HAAA patients. These findings suggest that haplo-HSCT is a feasible treatment option for HAAA when HLA-matched donors are not available, with low risks of transplant-related mortality and complications.
ANNALS OF HEMATOLOGY
(2022)
Article
Immunology
Shotaro Tabata, Hiroki Hosoi, Shogo Murata, Satomi Takeda, Toshiki Mushino, Takashi Sonoki
Summary: The development of autoimmune diseases following COVID-19 infections or vaccinations has been reported, but the exact relationship between vaccines and autoimmune diseases remains unclear. This case suggests that measuring vaccine antibody titers before and after allogeneic HSCT may provide clues to the pathogenesis of vaccine-related autoimmune diseases. Further evaluations are needed to assess the associations between vaccines and autoimmune diseases such as aplastic anemia.
JOURNAL OF AUTOIMMUNITY
(2022)
Article
Immunology
Xiaoyu Zhang, Wenrui Yang, Donglin Yang, Jialin Wei, Ping Zhang, Sizhou Feng, Erlie Jiang, Li Zhang, Yi He, Fengkui Zhang, Mingzhe Han
Summary: Hepatitis-associated aplastic anemia is a rare type of acquired aplastic anemia characterized by bone marrow failure following hepatitis. The study analyzed the outcomes of severe HAAA patients treated with immunosuppressive therapy, matched-sibling donor hematopoietic stem cell transplantation, or haploidentical-donor HSCT as the first-line treatment. The findings showed that HSCT resulted in a higher and more sustained hematologic response compared to IST. Overall survival rates were similar among the treatment groups, but HSCT demonstrated a trend of better failure-free survival rates, particularly in young patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Letter
Hematology
Kohei Hosokawa, Shinji Nakao
ANNALS OF HEMATOLOGY
(2022)
Article
Hematology
Kohei Hosokawa, Hiroki Mizumaki, Takeshi Yoroidaka, Hiroyuki Maruyama, Tatsuya Imi, Noriaki Tsuji, Ryota Urushihara, Mikoto Tanabe, Yoshitaka Zaimoku, Mai Anh Thi Nguyen, Dung Cao Tran, Ken Ishiyama, Hirohito Yamazaki, Takamasa Katagiri, Hiroyuki Takamatsu, Kazuyoshi Hosomichi, Atsushi Tajima, Fumihiro Azuma, Seishi Ogawa, Shinji Nakao
Article
Hematology
Kohei Hosokawa, Go Aoki, Kinya Ohata, Hiroyuki Takamatsu, Noriharu Nakagawa, Tatsuya Imi, Noriko Iwaki, Kiyoaki Ito, Mitsuhiro Kawano, Takashi Nakamura, Masato Takamori, Ken Ishiyama, Yukio Kondo, Hirohito Yamazaki, Shinji Nakao
Summary: Hyperbaric oxygen therapy is a feasible option for treating viral hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation, and it is more effective than conventional therapy for HC patients.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2021)
Article
Oncology
Takeshi Yoroidaka, Kohei Hosokawa, Tatsuya Imi, Hiroki Mizumaki, Takamasa Katagiri, Ken Ishiyama, Hirohito Yamazaki, Fumihiro Azuma, Yasuhito Nanya, Seishi Ogawa, Shinji Nakao
Summary: The study found that HLA(-) HSPCs differ from GPI(-) HSPCs in both hierarchical stage and sensitivity to immune attacks in acquired aplastic anemia (AA).
Article
Hematology
Noriharu Nakagawa, Ken Ishiyama, Mikoto Tanabe, Takeshi Yoroidaka, Hiroki Mizumaki, Tatsuya Imi, Yoshitaka Zaimoku, Hiroyuki Maruyama, Kohei Hosokawa, Hirohito Yamazaki, Shinji Nakao
Summary: This study observed 43 patients with AA who had previously received chemotherapy or radiotherapy, and found that IST treatment can improve the survival rate of patients, especially when the original solid tumors are in remission.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Review
Oncology
Goshi Hagiwara, Masahisa Arahata, Kohei Hosokawa, Masaya Shimojima, Shinji Nakao
Summary: This case report presents a patient with POEMS syndrome complicated by CHF initially mimicking cardiac amyloidosis, but eventually diagnosed correctly after significant improvement of CHF with chemotherapy. It highlights the importance of careful evaluation and consideration of differential diagnoses in patients with POEMS syndrome presenting with CHF.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Mikoto Tanabe, Kohei Hosokawa, Mai Anh Thi Nguyen, Noriharu Nakagawa, Kana Maruyama, Noriaki Tsuji, Ryota Urushihara, Luis Espinoza, Mahmoud Elbadry, Md Mohiuddin, Takamasa Katagiri, Masanori Ono, Hiroshi Fujiwara, Kazuhisa Chonabayashi, Yoshinori Yoshida, Hirohito Yamazaki, Atsushi Hirao, Shinji Nakao
Summary: CD109 suppresses TGF-beta signaling in HSPCs, and the lack of CD109 may increase the sensitivity of PIGA-mutated HSPCs to TGF-beta, leading to the preferential commitment of erythroid progenitor cells to mature red blood cells in immune-mediated BM failure.
Article
Hematology
Regis Peffault de Latour, Kohei Hosokawa, Antonio Maria Risitano
Summary: Paroxysmal nocturnal hemoglobinuria (PNH) is a disease characterized by intravascular hemolysis, thrombosis, and bone marrow failure. The introduction of the anti-C5 monoclonal antibody eculizumab has significantly improved treatment outcomes, but it requires lifelong infusion and some patients still experience anemia. New anti-C5 agents and proximal complement inhibitors offer potential alternatives for improving the efficacy of treatment.
SEMINARS IN HEMATOLOGY
(2022)
Article
Oncology
Noriaki Tsuji, Kohei Hosokawa, Ryota Urushihara, Mikoto Tanabe, Takamasa Katagiri, Tatsuhiko Ozawa, Hiroyuki Takamatsu, Ken Ishiyama, Hirohito Yamazaki, Hiroyuki Kishi, Seishi Ogawa, Shinji Nakao
Summary: This study demonstrates that antigens presented by HSPCs on HLA-DR15 may lead to abnormal activation of CD4(+) T cells and immune escape in the development of AA. Immune response-related gene expression is downregulated in DR(-) cells compared to HLA-I(-) HSPCs.
Article
Hematology
Hideyuki Nakazawa, Kaoko Sakai, Akiko Ohta, Naohito Fujishima, Akira Matsuda, Kohei Hosokawa, Fumi Nakamura, Shinji Nakao, Kinuko Mitani, Fumihiro Ishida
Summary: Acquired pure red cell aplasia (PRCA) is a rare syndrome characterized by anemia with reticulocytopenia and a marked reduction in erythroid precursors. A recent epidemiological study in Japan revealed an annual incidence of PRCA at 1.06 per million, with a higher proportion of female patients and a majority of cases being idiopathic. PRCA accounted for approximately 20% of the incidence of aplastic anemia during the same period.
Article
Oncology
Tiina Kelkka, Mikko Tyster, Sofie Lundgren, Xingmin Feng, Cassandra Kerr, Kohei Hosokawa, Jani Huuhtanen, Mikko Keranen, Bhavisha Patel, Toru Kawakami, Yuka Maeda, Otso Nieminen, Tiina Kasanen, Pasi Aronen, Bhagwan Yadav, Hanna Rajala, Hideyuki Nakazawa, Taina Jaatinen, Eva Hellstrom-Lindberg, Seishi Ogawa, Fumihiro Ishida, Hiroyoshi Nishikawa, Shinji Nakao, Jaroslaw Maciejewski, Neal S. Young, Satu Mustjoki
Summary: A novel autoantibody, aCOX-2 Ab, was found in immune aplastic anemia, and it is associated with age and the HLA-DRB1*15:01 genotype, suggesting its potential value as a disease biomarker.
Article
Hematology
Ken Ishiyama, Yuji Yonemura, Tatsuya Kawaguchi, Kohei Hosokawa, Chiharu Sugimori, Yasutaka Ueda, Hiroyuki Takamori, Naoshi Obara, Hideyoshi Noji, Yukari Shirasugi, Kiyoshi Ando, Tsutomu Shichishima, Haruhiko Ninomiya, Shigeru Chiba, Jun-ichi Nishimura, Yuzuru Kanakura, Shinji Nakao
Summary: In this observational study, it was found that 52.6% of patients with acquired aplastic anemia (AA) and 13.7% of patients with myelodysplastic syndrome (MDS) had glycosylphosphatidylinositol-anchored protein-deficient (GPI[-]) cell populations (PNH-type cells). Furthermore, the presence of =1% PNH-type granulocytes was found to predict a higher likelihood of PNH-type cell expansion. This study provides valuable insights into the epidemiological characteristics and clinical significance of PNH-type cells in AA and MDS patients.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Immunology
Shinichi Nakanuma, Ryosuke Gabata, Mitsuyoshi Okazaki, Akihiro Seki, Kohei Hosokawa, Tadafumi Yokoyama, Kaoru Katano, Hiroaki Sugita, Tomokazu Tokoro, Satoshi Takada, Isamu Makino, Takumi Taniguchi, Kenichi Harada, Shintaro Yagi
Summary: Hemophagocytic lymphohistiocytosis (HLH) is a rare but lethal complication of liver transplantation, characterized by macrophage activation and cytokine storm. This study found that cytokine analysis related to macrophage activation can be useful for diagnosing and predicting HLH and MAS in patients with liver transplantation.
TRANSPLANTATION PROCEEDINGS
(2023)
Letter
Oncology
Hiroki Mizumaki, Dung Cao Tran, Kohei Hosokawa, Kazuyoshi Hosomichi, Yoshitaka Zaimoku, Hiroyuki Takamatsu, Hirohito Yamazaki, Ken Ishiyama, Rena Yamazaki, Hiroshi Fujiwara, Atsushi Tajima, Shinji Nakao
BLOOD CANCER JOURNAL
(2023)
Article
Hematology
Kohei Hosokawa, Shinji Nakao
Summary: Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired hematopoietic stem cell disorder caused by a PIGA gene mutation. Clonal expansion of GPI(-) cells is common in PNH, but tiny GPI(-) cell populations can also be found in healthy individuals. In patients with acquired aplastic anemia, the expansion of PNH clones is related to immune attack on hematopoietic stem cells. However, the mechanisms underlying the selection and expansion of GPI(-) cells remain unclear.
SEMINARS IN HEMATOLOGY
(2022)