Article
Hematology
Alexis Leonard, Akshay Sharma, Naoya Uchida, David Stroncek, Sandhya R. Panch, Kamille West, Eoghan Molloy, Thomas E. Hughes, Sara Hauffe, Tiffani Taylor, Courtney Fitzhugh, Jane S. Hankins, Megan Wilson, Shengdar Q. Tsai, Mitchell J. Weiss, Matthew Hsieh, John F. Tisdale
Summary: Recent studies have shown that plerixafor mobilization and apheresis in patients with sickle cell disease can safely collect sufficient CD34(+) hematopoietic stem cells for clinical gene therapy applications. The quantity of CD34(+) cells mobilized by plerixafor varies among SCD patients for unknown reasons, and factors influencing this yield include age, baseline and pre-apheresis CD34(+) cell counts, and disease severity markers unique to SCD.
Article
Oncology
Jingjing Wen, Qiaolin Zhou, Lin Shi, Fang Xu, Yiping Liu, Jing Su, Ya Zhang, Wen Qu, Jing Yue
Summary: The study aimed to evaluate the efficacy of mecapegfilgrastim as a mobilization agent for hematopoietic stem cell transplantation in patients with hematologic malignancies and investigate potential factors associated with mobilization failure. The results showed that mecapegfilgrastim had comparable mobilization efficacy to rhG-CSF in patients with hematologic malignancies, and the combination with plerixafor was feasible and effective. Furthermore, patients with more than 5 cycles of chemotherapy, a precollection white blood cell count lower than 32 x 10^9/L, and a precollection lymphocyte count less than 1.7 times the premobilization lymphocyte count had a higher risk of mobilization failure.
Article
Cell & Tissue Engineering
Tso-Fu Wang, Yu-Shan Liou, Shang-Hsien Yang, Guan-Ling Lin, Ya-Wen Chiang, Te-Sheng Lien, Chi-Cheng Li, Jen-Hung Wang, Hsin-Hou Chang, Der-Shan Sun
Summary: P-selectin is identified as a critical factor for G-CSF-induced HSC mobilization. Blocking the interaction between P-selectin and PSGL-1 can facilitate the mobilization of HSCs from bone marrow to peripheral circulation.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biophysics
Kristina Hoelig, Helmuth Schmidt, Gero Huetter, Michael Kramer, Raphael Teipel, Katharina Heidrich, Kristin Zimmer, Falk Heidenreich, Matthias Blechschmidt, Tigran Torosian, Rainer Ordemann, Frank Kroschinsky, Elke Ruecker-Braun, Laszlo Gopsca, Eva Maria Wagner-Drouet, Uta Oelschlaegel, Alexander H. Schmidt, Martin Bornhaeuser, Gerhard Ehninger, Johannes Schetelig
Summary: In allogeneic PBSC donors with poor mobilization response to G-CSF, the study found that the use of plerixafor (P) can effectively increase the yield of CD34+ cells, helping to achieve the target cell count without severe adverse events.
BONE MARROW TRANSPLANTATION
(2021)
Review
Hematology
Tomohide Suzuki, Shinichi Ishii, Yoshio Katayama
Summary: This review introduced the mechanistic insights on the mobilization of hematopoietic stem cells (HSCs) induced by granulocyte colony-stimulating factor (G-CSF) from the aspect of the sympathetic nervous system (SNS). Recent findings reveal the balance between promotion and suppression pathways regulated by the SNS determines the efficiency of mobilization. Understanding the regulatory mechanisms of G-CSF-induced mobilization and its clinical implications can aid in the treatment and research of hematopoietic stem cells.
CURRENT OPINION IN HEMATOLOGY
(2023)
Article
Oncology
Zhijuan Zhu, Xiaofan Li, Yiping Liu, Ping Chen, Xianling Chen, Hua Li, Jiafu Huang, Yuanzhong Chen, Nainong Li
Summary: Efficient and safe mobilization of CD34+ hematopoietic stem cells was achieved in patients with MM using Etoposide + Cytarabine plus G-CSF, which could be considered for high-risk MM patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biophysics
Carlo Lazzaro, Luca Castagna, Francesco Lanza, Daniele Laszlo, Giuseppe Milone, Luca Pierelli, Riccardo Saccardi
Summary: The study compared the cost-effectiveness of using G-CSF alone versus high-dose cyclophosphamide + G-CSF mobilization in Italian multiple myeloma patients eligible for autograft. The findings revealed that G-CSF alone was a more cost-effective option, demonstrating incremental savings and a higher probability of successful apheresis.
BONE MARROW TRANSPLANTATION
(2021)
Article
Biology
Astrid S. Jorgensen, Viktorija Daugvilaite, Katia De Filippo, Christian Berg, Masa Mavri, Tau Benned-Jensen, Goda Juzenaite, Gertrud Hjorto, Sara Rankin, Jon Vabeno, Mette M. Rosenkilde
Summary: Plerixafor exhibits biased action by reversing the CXCL12 gradient across the bone marrow endothelium and showing superior therapeutic effect in HSC mobilization. In contrast, AMD11070, despite having superior antagonism, does not perform as effectively in HSC mobilization in vivo.
COMMUNICATIONS BIOLOGY
(2021)
Article
Medicine, General & Internal
Tso-Fu Wang, Yu-Shan Liou, Hsin-Hou Chang, Shang-Hsien Yang, Chi-Cheng Li, Jen-Hung Wang, Der-Shan Sun
Summary: This study investigated the correlation between body mass index (BMI), proinflammatory cytokine levels, and hematopoietic stem cell (HSC) mobilization triggered by granulocyte colony-stimulating factor (G-CSF). The results showed that BMI was positively correlated with G-CSF-induced HSC mobilization, and good mobilizers had higher BMI and proinflammatory cytokine levels compared to poor mobilizers.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Hematology
Chang Li, Kevin A. Goncalves, Tamas Rasko, Amit Pande, Sucheol Gil, Zhinan Liu, Zsuzsanna Izsvak, Thalia Papayannopoulou, John C. Davis, Hans-Peter Kiem, Andre Lieber
Summary: A new in vivo hemopoietic stem cell gene therapy approach has been developed without the need for myelosuppressive conditioning and autologous HSC transplantation, involving HSC mobilization and a helper-dependent adenovirus vector system. The mobilization regimen using truncated GRO-beta and plerixafor resulted in robust HSC mobilization with less toxicity. In a mouse model, in vivo HSC transduction after mobilization led to complete correction of thalassemia, demonstrating the potential for long-term HSC repopulation.
Article
Biochemistry & Molecular Biology
Weimin Tang, Sudipta Panja, Chinmay M. Jogdeo, Siyuan Tang, Ao Yu, David Oupicky
Summary: This study investigates the impact of charge and hydrophilicity of polymers on their accumulation in injured kidneys and demonstrates that polymers with hydroxyl group modification show the least accumulation. These findings provide important guidance for the development of targeted drug carriers for AKI therapy.
Article
Pharmacology & Pharmacy
Shao-Yu Tang, Yi-Chin Lee, Chien-Wei Tseng, Po-Hsun Huang, Ko-Lin Kuo, Der-Cherng Tarng
Summary: This study found that G-CSF can improve angiogenic function in patients with peripheral arterial disease (PAD) associated with chronic kidney disease (CKD) through a hypoxia/IL-10 signaling cascade.
Article
Hematology
Donghua He, Chunting Zhu, Xing Guo, Xin Huang, Xiaoyan Han, Gaofeng Zheng, Yi Zhao, Yang Yang, Wenjun Wu, Jimei Ge, Enfan Zhang, Jingsong He, Zhen Cai
Summary: This study assessed the efficacy of residual plerixafor for second-day stem cell mobilization in multiple myeloma patients. The use of residual plerixafor was found to be an economical, efficient, and clinically feasible method.
TRANSFUSION AND APHERESIS SCIENCE
(2023)
Article
Hematology
Junjie Cao, Aizhen Li, Renzhi Pei, Ying Lu, Dong Chen, Xiaohong Du, Xuhui Liu, Shuangyue Li, Peipei Ye
Summary: This study showed that a single dosage of 6 mg pegfilgrastim was effective and safe in mobilizing CD34+ cells for allogeneic hematopoietic stem cell transplantation. Compared to the use of G-CSF, pegfilgrastim led to higher CD34+ cell yield in leukapheresis and a potential decrease in the incidence of acute graft-versus-host disease (aGVHD). The higher proportion of myeloid-derived suppressor cells (MDSCs) in the pegfilgrastim group may contribute to this decrease in aGVHD, but further research is needed to explore this relationship.
ANNALS OF HEMATOLOGY
(2023)
Article
Endocrinology & Metabolism
Xiaoying Lin, Hong Wang, Xiaolan Li
Summary: The study found that mobilization of endogenous EPCs by AMD3100 combined with G-CSF enhanced complete healing and neovascularization in full-thickness wounds of db/db mice, possibly through the SDF-1/CXCR4 axis. This provides a novel method for diabetic wound healing and tissue regeneration.
DIABETES & VASCULAR DISEASE RESEARCH
(2021)
Article
Immunology
Emilie Bourdonnay, Zbigniew Zaslona, Loka Raghu Kumar Penke, Jennifer M. Speth, Daniel J. Schneider, Sally Przybranowski, Joel A. Swanson, Peter Mancuso, Christine M. Freeman, Jeffrey L. Curtis, Marc Peters-Golden
JOURNAL OF EXPERIMENTAL MEDICINE
(2015)
Article
Immunology
Jennifer M. Speth, Emilie Bourdonnay, Loka Raghu Kumar Penke, Peter Mancuso, Bethany B. Moore, Jason B. Weinberg, Marc Peters-Golden
JOURNAL OF IMMUNOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Daniel J. Schneider, Jennifer M. Speth, Loka R. Penke, Scott H. Wettlaufer, Joel A. Swanson, Marc Peters-Golden
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Medicine, Research & Experimental
Pratibha Singh, Jonathan Hoggatt, Malgorzata M. Kamocka, Khalid S. Mohammad, Mary R. Saunders, Hongge Li, Jennifer Speth, Nadia Carlesso, Theresa A. Guise, Louis M. Pelus
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Medicine, Research & Experimental
Loka R. Penke, Jennifer M. Speth, Vijaya L. Dommeti, Eric S. White, Ingrid L. Bergin, Marc Peters-Golden
JOURNAL OF CLINICAL INVESTIGATION
(2018)
Article
Hematology
Jennifer M. Speth, Jonathan Hoggatt, Pratibha Singh, Louis M. Pelus
Article
Oncology
S. Fukuda, J. Hoggatt, P. Singh, M. Abe, J. M. Speth, P. Hu, E. M. Conway, G. Nucifora, S. Yamaguchi, L. M. Pelus
Article
Biochemistry & Molecular Biology
Christina Draijer, Jennifer M. Speth, Loka R. K. Penke, Zbigniew Zaslona, Joseph D. Bazzill, Njira Lugogo, Yvonne J. Huang, James J. Moon, Marc Peters-Golden
Article
Multidisciplinary Sciences
Loka R. Penke, Hideyasu Ouchi, Jennifer M. Speth, Njira Lugogo, Yvonne J. Huang, Steven K. Huang, Marc Peters-Golden
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Loka Raghu Kumar Penke, Jennifer Speth, Scott Wettlaufer, Christina Draijer, Marc Peters-Golden
Summary: This study evaluated the therapeutic efficacy of the proteasomal inhibitor bortezomib (BTZ) and found that it has antifibrotic actions in lung fibrosis. Surprisingly, these actions are independent of proteasome inhibition and instead mediated by the induction of dual-specificity protein phosphatase 1 (DUSP1).
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Loka R. Penke, Jennifer M. Speth, Steven K. Huang, Sean M. Fortier, Jared Baas, Marc Peters-Golden
Summary: This study found that the transcription factor KLF4 is suppressed in fibroblasts from fibrotic lung. Gain-and loss-of-function studies showed that KLF4 can inhibit fibroblast proliferation, collagen synthesis, and differentiation to myofibroblasts, while restoring their sensitivity to apoptosis. A small molecule inducer of KLF4 was able to restore its expression in fibrotic fibroblasts and induce resolution of fibrosis. These findings highlight the importance of KLF4 as a negative regulator of fibroblast activation.
Article
Medicine, Research & Experimental
Jennifer M. Speth, Loka R. Penke, Joseph D. Bazzill, Kyung Soo Park, Rafael Gil de Rubio, Daniel J. Schneider, Hideyasu Ouchi, James J. Moon, Venkateshwar G. Keshamouni, Rachel L. Zemans, Vibha N. Lama, Douglas A. Arenberg, Marc Peters-Golden
Meeting Abstract
Critical Care Medicine
J. Speth, L. Penke, J. D. Bazzill, J. J. Moon, M. Peters-Golden
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Meeting Abstract
Critical Care Medicine
L. Penke, H. Ouchi, C. Draijer, B. Hu, J. Speth, S. Phan, M. Peters-Golden
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Review
Cell Biology
Daniel J. Schneider, Jennifer M. Speth, Marc Peters-Golden
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2016)
