Article
Cardiac & Cardiovascular Systems
Xi Lou, Yawen Tang, Lei Ye, Danielle Pretorius, Vladimir G. Fast, Asher M. Kahn-Krell, Jue Zhang, Jianhua Zhang, Aijun Qiao, Gangjian Qin, Timothy Kamp, James A. Thomson, Jianyi Zhang
Summary: The study demonstrates that human cardiac muscle patches containing three types of cardiac cells derived from human pluripotent stem cells significantly improved cardiac function, reduced infarct size, and promoted cardiac maturation in a pig model of myocardial infarction. The inclusion of cardiac fibroblasts in the cardiac patches enhanced the maturity and function of cardiac muscle cells. Transplantation of cardiac patches with cardiac fibroblasts resulted in better cardiac recovery and engraftment compared to patches without fibroblasts.
CARDIOVASCULAR RESEARCH
(2023)
Article
Polymer Science
Maryam Torabi, Mohammad Foad Abazari, Shohreh Zare Karizi, Mina Kohandani, Nazanin Hajati-Birgani, Sara Norouzi, Fatemeh Nejati, Alireza Mohajerani, Tahereh Rahmati, Zakiye Mokhames
Summary: In this study, PLGA and PRP were used to fabricate nanofibrous scaffolds, and it was found that iPSCs cultured on PLGA-PRP nanofibers were more likely to differentiate into cardiomyocytes compared to PLGA. Multidimensional evaluations at the morphological, molecular gene, and protein expression levels indicated the potential of PLGA-PRP in cardiac tissue engineering.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Article
Cell & Tissue Engineering
Rida-e-Maria Qazi, Irfan Khan, Kanwal Haneef, Tuba Shakil Malick, Nadia Naeem, Waqas Ahmad, Asmat Salim, Sadia Mohsin
Summary: This study investigates the role of collagen scaffold and zebularine in the differentiation of rat bone marrow-derived MSCs and their subsequent in vivo effects. The results show that the combination of collagen scaffold and zebularine treatment enhances cardiac differentiation potential of MSCs and improves cardiac function.
WORLD JOURNAL OF STEM CELLS
(2022)
Article
Medicine, Research & Experimental
Suet Yen Chong, Olga Zharkova, Siti Maryam J. M. Yatim, Xiaoyuan Wang, Xiong Chang Lim, Chenyuan Huang, Chia Yee Tan, Jianming Jiang, Lei Ye, Michelle Siying Tan, Veronique Angeli, Henri H. Versteeg, Mieke Dewerchin, Peter Carmeliet, Carolyn S. P. Lam, Mark Y. Chan, Dominique P. V. de Kleijn, Jiong-Wei Wang
Summary: The study found that the TF cytoplasmic domain exacerbates post-infarct cardiac injury and adverse LV remodeling by regulating inflammation and angiogenesis. Targeted inhibition of TF cytoplasmic domain-mediated intracellular signaling may ameliorate post-infarct LV remodeling.
Article
Cardiac & Cardiovascular Systems
Jiao Jiao, Shujie He, Yiqiu Wang, Yuzhi Lu, Muyang Gu, Dan Li, Tingting Tang, Shaofang Nie, Min Zhang, Bingjie Lv, Jingyong Li, Ni Xia, Xiang Cheng
Summary: The study demonstrates that Bregs can limit ventricular remodeling after myocardial infarction by reducing CCR2-mediated monocyte recruitment and mobilization.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Review
Cell Biology
Vineeta Sharma, Sanat Kumar Dash, Kavitha Govarthanan, Rekha Gahtori, Nidhi Negi, Mahmood Barani, Richa Tomar, Sudip Chakraborty, Santosh Mathapati, Dillip Kumar Bishi, Poonam Negi, Kamal Dua, Sachin Kumar Singh, Rohit Gundamaraju, Abhijit Dey, Janne Ruokolainen, Vijay Kumar Thakur, Kavindra Kumar Kesari, Niraj Kumar Jha, Piyush Kumar Gupta, Shreesh Ojha
Summary: Myocardium Infarction (MI) is a leading cause of death worldwide, with pharmacological interventions not being the forefront in improving outcomes. Stem cell-based tissue engineering approaches have shown promise in regenerating the infarcted myocardium. Research using microfluidic devices has provided insights into the pathophysiology of MI, aiding in the differentiation of stem cells and engineering of scaffolds mimicking native cardiac conditions. Delivery of stem cell-loaded engineered scaffolds to the infarction site remains a challenge for future clinical translation.
Review
Biochemistry & Molecular Biology
Shudong Zhao, Jishizhan Chen, Lei Wu, Xin Tao, Naheem Yaqub, Jinke Chang
Summary: Skeletal muscle, responsible for crucial functions in the body, can suffer from severe injuries leading to volumetric muscle loss (VML). Tissue-engineered muscles using induced pluripotent stem cells (iPSCs) offer a potential solution to restore muscle function. iPSCs, with their pluripotency and self-renewal capacity, can be used to construct tissue-engineered artificial skeletal muscles for various applications. These iPSC-based models also have the potential to revolutionize drug discovery and reduce reliance on animal models. This review provides an overview of iPSCs in tissue-engineered skeletal muscles, discussing advancements, challenges, and considerations for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Miguel J. S. Ferreira, Fabrizio E. Mancini, Paul A. Humphreys, Leona Ogene, Michael Buckley, Marco A. N. Domingos, Susan J. Kimber
Summary: The use of human pluripotent stem cells in skeletal tissue engineering has shown promising results, with various methods being used for generating cartilage and bone. Emphasis is placed on generating authentic tissues rather than just differentiated cells, and new technologies are being developed to overcome challenges in this field. It is likely that cost-effective and efficacious engineered constructs using human pluripotent stem cells will be available for skeletal repair in the near future.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Junjun Li, Xiang Qu, Li Liu, Lingjun Li, Ying Hua, Jingbo Zhang, Masako Ishida, Noriko Yoshida, Akiko Tabata, Nagako Sougawa, Emiko Ito, Noriko Mochizuki-Oda, Akima Harada, Takuji Kawamura, Ryohei Matsuura, Yingzhe Wang, Keisuke Morishima, Shigeru Miyagawa, Yoshiki Sawa
Summary: A three-dimensional multilayer fibrous scaffold was developed for the culture of hiPSC-derived cardiac tissue, which showed improved contractile properties and cytokine secretion compared to the control group. When used in a myocardial infarction model, the multilayer group demonstrated improved functional recovery and reduced fibrosis. These results suggest that higher doses of hiPSC-derived cardiomyocytes may have enhanced therapeutic effects in the treatment of myocardial infarction.
ADVANCED FIBER MATERIALS
(2023)
Article
Engineering, Biomedical
Shuai Deng, Xiaoyu Zhao, Yanlun Zhu, Ning Tang, Rongliang Wang, Xuerao Zhang, Fuyang Qu, Yi-Ping Ho, Wayne Yuk-Wai Lee, Jiansu Chen, Mingqiang Li, Yu Tao, Hon Fai Chan
Summary: Liver tissue engineering is advanced through the fabrication of collagen type I microspheres, enabling efficient hepatic differentiation of stem cells and assembly of prevascularized liver tissue. The microspheres exhibit high uniformity and can self-assemble with endothelial cells to form high cell-packing density liver tissue. This technology has potential applications in regenerative medicine, drug screening, and in vitro liver modeling.
Article
Engineering, Biomedical
Jiesi Luo, Xiangyu Shi, Yuyao Lin, Yifan Yuan, Mehmet H. Kural, Juan Wang, Matthew W. Ellis, Christopher W. Anderson, Shang-Min Zhang, Muhammad Riaz, Laura E. Niklason, Yibing Qyang
Summary: The study successfully developed xenogeneic-free conditions for deriving functional endothelial cells from human induced pluripotent stem cells (XF-hiPSC-ECs), which exhibited similar marker expression and function to human primary ECs. Moreover, the XF-hiPSC-ECs responded to shear stress with typical cell alignment and gene expression, and were successfully used to endothelialize decellularized human vessels in a dynamic bioreactor system, advancing the potential clinical application of tissue engineered vascular grafts.
ACTA BIOMATERIALIA
(2021)
Article
Cardiac & Cardiovascular Systems
Klaus Neef, Florian Drey, Vera Lepperhof, Thorsten Wahlers, Jurgen Hescheler, Yeong-Hoon Choi, Tomo Saric
Summary: Co-transplantation of iPS-CM and MSC improves cardiac regeneration after myocardial damage, demonstrating the potential of combining multiple cell types for increasing the efficacy of future cardiac cell therapies. MSCs enhance the retention of iPS-CM and result in a significantly higher left ventricular ejection fraction (LVEF) when transplanted together.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Ahmet Demirkiran, Rob J. van der Geest, Luuk H. G. A. Hopman, Lourens F. H. J. Robbers, M. Louis Handoko, Robin Nijveldt, John P. Greenwood, Sven Plein, Pankaj Garg
Summary: LV hemodynamic assessment by LV blood flow kinetic energetics in STEMI patients shows a significant inverse association with adverse LV-remodeling. Late-diastolic LV blood flow kinetic energetics early after acute MI is independently associated with adverse LV-remodeling.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Mengcheng Shen, Thomas Quertermous, Michael P. Fischbein, Joseph C. Wu
Summary: The developmental origin of vascular smooth muscle cells plays a crucial role in regional susceptibility and resistance to vascular diseases. Patient induced pluripotent stem cell-derived VSMCs are valuable for research and therapeutic applications, but the heterogeneity in developmental origins poses challenges.
CIRCULATION RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Hug Aubin, Lenard Rath, Alexandra Vey, Vera Schmidt, Mareike Barth, Elvira Weber, Artur Lichtenberg, Payam Akhyari
Summary: This study evaluated the potential of using a cardiac decellularized extracellular matrix (dECM) scaffold for supporting injured myocardium after myocardial infarction (MI). The results showed that ventricular stabilization with the dECM scaffold improved cardiac remodeling and altered gene expression patterns in the infarcted myocardium.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Matthew Miyamoto, Peter Andersen, Edrick Sulistio, Xihe Liu, Sean Murphy, Suraj Kannan, Lucy Nam, William Miyamoto, Emmanouil Tampakakis, Narutoshi Hibino, Hideki Uosaki, Chulan Kwon
Summary: The Notch pathway plays crucial roles in cell-fate decisions and can exert its activity through canonical and noncanonical pathways. Increased levels of Notch intracellular domain inhibit heart development in early mesoderm, while overexpression in late cardiac progenitor cells can increase heart size.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Yuki Miura, Min-Yin Li, Omer Revah, Se-Jin Yoon, Genta Narazaki, Sergiu P. Pasca
Summary: This article describes a method for generating human brain assembloids and studying neural circuits. By converting human pluripotent stem cells into brain region-specific organoids, which are then fused and integrated to form assembloids, complex cell-cell interactions, circuit formation, and maturation can be studied using self-organization. A series of assays, including viral labeling and retrograde tracing, 3D live imaging, and electrophysiology, are used to model and manipulate neural circuits in the assembloids. These approaches are valuable for understanding human-specific aspects of neural circuit assembly and modeling neurodevelopmental disorders.
Article
Neurosciences
Azumi Noguchi, Kenji Ito, Yuichi Uosaki, Maky Ideta-Otsuka, Katsuhide Igarashi, Hideyuki Nakashima, Toshikazu Kakizaki, Ruri Kaneda, Hideki Uosaki, Yuchio Yanagawa, Kinichi Nakashima, Hirokazu Arakawa, Takumi Takizawa
Summary: During neuronal maturation, the genomic locus 14qD2 is relocated from the nuclear periphery to the interior, accompanied by a decrease in lamin B1 expression. Overexpression of lamin B1 prevents the relocation of the locus and suppresses the transcription of the Egr3 gene.
NEUROSCIENCE RESEARCH
(2021)
Article
Cell Biology
William J. Kowalski, Iris H. Garcia-Pak, Wenling Li, Hideki Uosaki, Emmanouil Tampakakis, Jizhong Zou, Yongshun Lin, Kira Patterson, Chulan Kwon, Yoh-Suke Mukouyama
Summary: Embryos without autonomic innervation experience sudden cardiac death. This study demonstrates the significant and complex role of sympathetic neurons in regulating cardiomyocyte development, including up-regulation of maturation-related genes, increased sarcomere organization and gap junctions, and enhanced transient amplitudes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Chihiro Nishiyama, Yuichi Saito, Akane Sakaguchi, Mari Kaneko, Hiroshi Kiyonari, Yuqing Xu, Yuichiro Arima, Hideki Uosaki, Wataru Kimura
Summary: The marsupial opossum is capable of maintaining cardiomyocyte proliferation and myocardial regeneration for at least 2 weeks after birth, which is the longest postnatal duration of such a capacity among mammals examined to date.
Article
Multidisciplinary Sciences
Takeshi Tokuyama, Hideki Uosaki, Ayumu Sugiura, Gen Nishitai, Keisuke Takeda, Shun Nagashima, Isshin Shiiba, Naoki Ito, Taku Amo, Satoshi Mohri, Akiyuki Nishimura, Motohiro Nishida, Ayumu Konno, Hirokazu Hirai, Satoshi Ishido, Takahiro Yoshizawa, Takayuki Shindo, Shingo Takada, Shintaro Kinugawa, Ryoko Inatome, Shigeru Yanagi
Summary: This study reveals that abnormal mitochondrial fragmentation and dysfunction are associated with aging-related heart diseases, and the E3 ubiquitin ligase MITOL can protect against these conditions by targeting the protein Drp1 for degradation. The findings suggest that activating OMMAD through MITOL can be a potential therapeutic strategy for treating aging-associated heart diseases.
Meeting Abstract
Cardiac & Cardiovascular Systems
Nawin Chanthra, Razan E. Ahmed, Tatsuya Anzai, Takeshi Tokuyama, Kumiko A. Iwabuchi, Yutaka Hanazono, Hideki Uosaki
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Multidisciplinary Sciences
Matthew Miyamoto, Suraj Kannan, Matthew J. Anderson, Xihe Liu, David Suh, Myo Htet, Biyi Li, Tejasvi Kakani, Sean Murphy, Emmanouil Tampakakis, Mark Lewandoski, Peter Andersen, Hideki Uosaki, Chulan Kwon
Summary: This study investigates the importance of the Wnt signaling pathway in heart development and the source of Wnt signals. Through genetic manipulation, single-cell RNA sequencing, and a precardiac organoid model, the authors demonstrate that cardiac progenitor cells can regulate the development of the second heart field through autoregulation of Wnt signaling. These findings have important implications for understanding the mechanisms of heart development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
Chika Watanabe, Hitoshi Osaka, Miyuki Watanabe, Akihiko Miyauchi, Eriko F. Jimbo, Takeshi Tokuyama, Hideki Uosaki, Yoshihito Kishita, Yasushi Okazaki, Takanori Onuki, Tomohiro Ebihara, Kenichi Aizawa, Kei Murayama, Akira Ohtake, Takanori Yamagata
Summary: Coenzyme Q10 (CoQ10) is involved in ATP production through electron transfer in the mitochondrial respiratory chain complex. The redox state of CoQ10 has been reported as a marker of the mitochondrial metabolic state. In this study, reduced and oxidized CoQ10 levels were measured in skin fibroblasts from mitochondrial disease patients, and the reduced/total CoQ10 ratio was determined. The results showed that the measurement of CoQ10 in fibroblasts can be useful for the diagnosis of primary CoQ10 deficiency, and the reduced/total CoQ10 ratio may reflect the metabolic status of mitochondrial disease.
MOLECULAR GENETICS AND METABOLISM REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Tomohiro Hino, Satoshi N. Omura, Ryoya Nakagawa, Tomoki Togashi, Satoru N. Takeda, Takafumi Hiramoto, Satoshi Tasaka, Hisato Hirano, Takeshi Tokuyama, Hideki Uosaki, Soh Ishiguro, Madina Kagieva, Hiroyuki Yamano, Yuki Ozaki, Daisuke Motooka, Hideto Mori, Yuhei Kirita, Yoshiaki Kise, Yuzuru Itoh, Satoaki Matoba, Hiroyuki Aburatani, Nozomu Yachie, Tautvydas Karvelis, Virginijus Siksnys, Tsukasa Ohmori, Atsushi Hoshino, Osamu Nureki
Summary: This study developed two activity-enhanced variants of AsCas12f through deep mutational scanning and structure-informed design, which exhibited genome-editing activities comparable to SpCas9 and AsCas12a in human cells. Cryo-EM structure analysis revealed the mechanism of the mutations, and the enAsCas12f variants packaged in AAV vectors demonstrated efficient knock-in/knock-out activities and transcriptional activation in mice.
Article
Cardiac & Cardiovascular Systems
Takeshi Shimamoto, Tatsuhiko Komiya, Takehiko Matsuo
Summary: This study reviewed single-center data and found that the turn-up anastomosis technique can effectively manage the anastomosis and hemostasis of type A acute aortic dissection, reducing complications during surgery.
ASIAN CARDIOVASCULAR & THORACIC ANNALS
(2023)
Article
Medicine, Research & Experimental
Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono, Osamu Nureki, Tsukasa Ohmori
Summary: The researchers have developed a base-editing approach using SpCas9-NG to correct a causative mutation in hemophilia B. They successfully corrected the F9 gene mutation in cells isolated from a patient and in mice, showing potential for this gene-editing approach as a therapy for hemophilia B. The study highlights the broad PAM flexibility of SpCas9-NG in base editing.
COMMUNICATIONS MEDICINE
(2023)
Article
Biochemical Research Methods
Sandeep Kambhampati, Sean Murphy, Hideki Uosaki, Chulan Kwon
Summary: Universal regulators during organ maturation were identified, offering the possibility for the maturation of stem cell-derived cells in vitro.
JOURNAL OF COMPUTATIONAL BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Akihiro Ikuta, Masanobu Ohya, Shunsuke Kubo, Kohei Osakada, Makoto Takamatsu, Kotaro Takahashi, Takeshi Maruo, Harumi Katoh, Takehiko Matsuo, Jota Nakano, Kazushige Kadota, Tatsuhiko Komiya, Yasushi Fuku
Summary: This study examined the association between renal morphological findings and changes in renal function in patients undergoing TAVI. The results showed a significant correlation between the renal cortex thickness index and changes in eGFR, suggesting that it could be used as a predictor for renal function changes in these patients.
Article
Biochemical Research Methods
Takafumi Miyamoto, Hideki Uosaki, Yuhei Mizunoe, Song-Iee Han, Satoi Goto, Daisuke Yamanaka, Masato Masuda, Yosuke Yoneyama, Hideki Nakamura, Naoko Hattori, Yoshinori Takeuchi, Hiroshi Ohno, Motohiro Sekiya, Takashi Matsuzaka, Fumihiko Hakuno, Shin-Ichiro Takahashi, Naoya Yahagi, Koichi Ito, Hitoshi Shimano
Summary: A synthetic biomolecular device, iCMM, was developed to manipulate mitochondrial morphology on a minute timescale, leading to changes in cellular behavior. This approach serves as a platform for developing more sophisticated devices to regulate biological systems, and could help uncover the biological significance of mitochondrial morphology in various cellular processes.
CELL REPORTS METHODS
(2021)
