Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Chongjun Xiao, Di Lu, Jinshuo Chen, Xiaoyan Chen, Huizhu Lin, Mudan Huang, Shimei Cheng, Yuge Wang, Qiuli Liu, Haiqing Zheng
Summary: Our study found that treatment with human olfactory mesenchymal stem cells (OMSC) delayed the onset and promoted neural functional recovery in a mouse model of EAE, possibly by suppressing CD4+IFN-gamma+ T cells. OMSC transplantation may be an alternative therapeutic option for neurological autoimmune diseases.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Guo-Guo Chu, Jing Wang, Zhi-Bin Ding, Jin-Zhu Yin, Li-Juan Song, Qing Wang, Jian-Jun Huang, Bao-Guo Xiao, Cun-Gen Ma
Summary: The study demonstrated that Hydroxyfasudil could alleviate the symptoms and pathological damage of experimental autoimmune encephalomyelitis (EAE), regulate the balance of peripheral T cells, inhibit inflammatory signaling cascades, promote anti-inflammatory factors in the central nervous system, and protect the blood-brain barrier. These findings suggest that Hydroxyfasudil may be a promising treatment for multiple sclerosis (MS).
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Behavioral Sciences
Jun-Mei Zhang, Hua Wang, Yu-Ying Fan, Feng-Hua Yang
Summary: MSCs treatment can increase the expression of cells in the oligodendrocyte lineage, laying a solid foundation for myelin regeneration.
BRAIN AND BEHAVIOR
(2021)
Article
Immunology
Xinyu Li, Yaojuan Chu, Rui Ma, Mengmeng Dou, Silu Li, Yifan Song, Ying Lv, Lin Zhu
Summary: Ferroptosis, a unique form of regulated cell death, may be involved in the pathogenesis of neurodegenerative disorders. This study provides evidence that ferroptosis is associated with oligodendrocyte loss and demyelination in an animal model of multiple sclerosis.
JOURNAL OF NEUROIMMUNOLOGY
(2022)
Article
Immunology
Xin Chen, Yanping Wang, Jiayu Ji, Changyu Li, Wei Zhuang, Jingyu Luo, Yu Shi, Qian Lin, Junfeng Wu, Anqi Li, Jing Wang, Yanting Meng, Sifan Zhang, Xiujuan Lang, Xijun Liu, Bo Sun, Hulun Li, Yumei Liu
Summary: The results of this study demonstrate that electroacupuncture treatment can suppress demyelination and inflammatory cell infiltration in a multiple sclerosis animal model, leading to a decrease in disease severity. Furthermore, electroacupuncture treatment increases the proportions of various stem cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Yuanhua Cai, Jocelyn A. A. Schroeder, Weiqing Jing, Cody Gurski, Calvin B. B. Williams, Shaoyuan Wang, Bonnie N. N. Dittel, Qizhen Shi
Summary: Targeting MOG expression to platelets using genetic engineering can prevent the development of multiple sclerosis and reduce disease severity, showing promise as a potential treatment approach.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Physiology
Natalia C. Osipchuk, Athena M. Soulika, Alla F. Fomina
Summary: Modulation of RyRs by administering RyR inhibitor dantrolene or introducing a gain-of-function RYR1-p.R163C mutation significantly affects the clinical progression of experimental autoimmune encephalomyelitis in mice. Daily administration of 5 or 10 mg/kg dantrolene reduces the severity of EAE clinical symptoms and dampens inflammation in the spinal cord. Mice with the gain-of-function RYR1-p.R163C mutation show faster and more severe development of EAE clinical symptoms.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Immunology
Maryam J. J. Tavaf, Azita Soltanmohammadi, Simin Zargarani, Esmaeil Yazdanpanah, Bizhan Sadighimoghaddam, Bahman Yousefi, Hamid R. R. Sameni, Dariush Haghmorad
Summary: This study utilized an EAE mouse model and found that low-dose and high-dose berberine can decrease the severity of EAE and alleviate its symptoms, possibly through increasing the number and function of Treg and Th2 cells, as well as its anti-inflammatory properties.
IMMUNITY INFLAMMATION AND DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Laetitia Pouzol, Nadege Baumlin, Anna Sassi, Melanie Tunis, Julia Marrie, Enrico Vezzali, Herve Farine, Ulrich Mentzel, Marianne M. Martinic
Summary: Current strategies for treating demyelinating diseases like multiple sclerosis focus on anti-inflammatory or immunomodulatory drugs. The CXCR7 receptor antagonist ACT-1004-1239 shows promise in reducing disease severity, decreasing neuroinflammation, and promoting myelin repair in experimental models.
Review
Neurosciences
Alessia Furgiuele, Marco Cosentino, Marco Ferrari, Franca Marino
Summary: Multiple sclerosis is a common chronic autoimmune disease of the central nervous system. Cannabinoids, particularly cannabidiol, may have anti-inflammatory and immunosuppressive effects, but further clinical evidence and research are needed to confirm their efficacy in treating MS.
JOURNAL OF NEUROIMMUNE PHARMACOLOGY
(2021)
Article
Biology
Xiao-Pei Ji, Yan-Zheng Gu, Hao Li, Zhou Yin, Nagam Varshithreddy, Ling -Tao Tang, Nan Li, Ying Zhang, Ming -Yuan Wang, Qin Shi, Si-Wei You, Xi-Ying Luan, Wan-Li Dong, Qi Fang, Xue-Guang Zhang, Qun Xue
Summary: This study investigated the immunomodulatory role and mechanism of B7-H4 on mouse mesenchymal stem cells in the development of experimental autoimmune encephalomyelitis (EAE). The results showed that silencing B7-H4 mitigated the immune-inhibitory effect of the cells and reduced the severity of inflammatory infiltrates and damages in EAE mice. This suggests that B7-H4 may play an immunomodulatory role in MSCs in vivo.
JOURNAL OF BIOLOGICAL RESEARCH-THESSALONIKI
(2022)
Article
Biochemistry & Molecular Biology
Dariush Haghmorad, Bahman Yousefi, Majid Eslami, Ali Rashidy-Pour, Mahdieh Tarahomi, Maryam Jadid Tavaf, Azita Soltanmohammadi, Simin Zargarani, Aleksandr Kamyshnyi, Valentyn Oksenych
Summary: This study investigated the efficacy of oral administration of Myelin Oligodendrocyte Glycoprotein (MOG) in the treatment of experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis (MS). The results showed that MOG administration, both before and after EAE induction, efficiently controlled EAE development by modulating T cell proliferation and reducing pro-inflammatory cytokines.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Immunology
Moritz J. Frenger, Christina Hecker, Mustafa Sindi, Andrea Issberner, Hans-Peter Hartung, Sven G. Meuth, Michael Dietrich, Philipp Albrecht
Summary: A step-by-step protocol for the semi-automatic evaluation of myeloid cells in cSLO images from mice was developed, revealing correlations between clinical parameters and cSLO results. This protocol offers a balance between fast data evaluation and accuracy, making it useful for research on inflammatory and degenerative CNS diseases involving the retina.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Anwar Fathollahi, Seyed Mahmoud Hashemi, Mostafa Haji Molla Hoseini, Sajjad Tavakoli, Elnaz Farahani, Farshid Yeganeh
Summary: Treatment with intranasal administration of MSC-SEV and MSC showed a significant decrease in clinical scores in EAE mice, with MSC-SEV being more effective than MSC. The therapy increased immunomodulatory responses, including an increase in the frequency of Foxp3+ CD25+ regulatory T cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Radiology, Nuclear Medicine & Medical Imaging
Jeff W. M. Bulte, Ali Shakeri-Zadeh
Summary: The efficacy of cancer vaccines using tumor antigen-primed dendritic cells (DCs) is influenced by the complexity of the immune response and various factors. Proton (H-1) and fluorine (F-19) magnetic resonance imaging (MRI) can be utilized to track and accurately inject therapeutic DCs, predicting vaccine effectiveness in advance.
MOLECULAR IMAGING AND BIOLOGY
(2022)
Article
Engineering, Biomedical
Yue Yuan, Congxiao Wang, Shreyas Kuddannaya, Jia Zhang, Dian R. Arifin, Zheng Han, Piotr Walczak, Guanshu Liu, Jeff W. M. Bulte
Summary: This study demonstrates that high-mannose N-linked glycans can serve as a biomarker for label-free tracking of transplanted human mesenchymal stromal cells (hMSCs) using mannose-weighted chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI). This label-free imaging method may facilitate the development and testing of cell therapies.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Antonina M. Araszkiewicz, Eduarda P. Oliveira, Terje Svendsen, Katarzyna Drela, Piotr Rogujski, Izabela Malysz-Cymborska, Michal Fiedorowicz, Rui L. Reis, Joaquim Miguel Oliveira, Piotr Walczak, Miroslaw Janowski, Barbara Lukomska, Luiza Stanaszek
Summary: Cell transplantation is a promising strategy for treating neurological disorders, but its effectiveness is limited by various challenges. This study developed alginate-based hydrogels as cell carriers, which improved the delivery accuracy and efficacy of transplanted cells. These hydrogels also enabled non-invasive magnetic resonance imaging (MRI) for monitoring the fate of the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Aline M. Thomas, Ethan Yang, Matthew D. Smith, Chengyan Chu, Peter A. Calabresi, Kristine Glunde, Peter C. M. van Zijl, Jeff W. M. Bulte
Summary: The study explores the utility of CEST MRI in monitoring disease activity in MS, showing that CEST signals correspond temporally with disease activity and correlate with cellular and metabolic composition of lymph nodes during the active stage of experimental autoimmune encephalomyelitis (EAE). Correlations were also found between CEST MRI biomarkers, immune activation, and LN metabolite levels.
JOURNAL OF NEUROINFLAMMATION
(2022)
Review
Radiology, Nuclear Medicine & Medical Imaging
Aline M. Thomas, Frederik Barkhof, Jeff W. M. Bulte
Summary: Imaging technology is an essential component in the management of multiple sclerosis, allowing for the evaluation of therapeutic efficacy. The development of molecular imaging probes has expanded our ability to assess and monitor multiple sclerosis and its treatments at the molecular level. However, despite the demonstrated utility of molecular imaging probes in multiple sclerosis applications, their use remains limited in both preclinical and clinical settings.
Review
Nanoscience & Nanotechnology
Sean Healy, Andris F. Bakuzis, Patrick W. Goodwill, Anilchandra Attaluri, Jeff W. M. Bulte, Robert Ivkov
Summary: Magnetic nanomaterials have potential for cancer nanotheranostics, but their physics complexities present challenges. Magnetic fluid hyperthermia (MFH) requires particle-imaging technology for real-time thermometry, and magnetic particle imaging (MPI) is the most promising technology to fulfill this requirement.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luiza Stanaszek, Piotr Rogujski, Katarzyna Drela, Michal Fiedorowicz, Piotr Walczak, Barbara Lukomska, Miroslaw Janowski
Summary: Amyotrophic lateral sclerosis (ALS) is a fatal disease with no effective therapy. This study investigated the transplantation of stem cells into immunodeficient mice with ALS, but did not find any therapeutic benefits in terms of animal survival or neurodegeneration. The lack of positive effects might be due to the inability of the transplanted cells to prevent cell loss.
Article
Chemistry, Multidisciplinary
Jeff W. M. Bulte, Chao Wang, Ali Shakeri-Zadeh
Summary: Superparamagnetic iron oxide (SPIO)-labeling of cells for magnetic resonance imaging (MRI) cell tracking has a long history, but studies have shown that the labeled cells may have different behaviors compared to unlabeled cells. With the emergence of magnetic particle imaging (MPI) cell tracking, there has been a renaissance in the formulation of SPIO nanoparticles optimized for MPI. Lessons learned from past experiences with SPIO-labeling for MRI could potentially facilitate the clinical translation of (combined) MRI/MPI cell tracking.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Oncology
Ruman Rahman, Miroslaw Janowski, Clare L. Killick-Cole, William G. B. Singleton, Emma Campbell, Piotr Walczak, Soumen Khatua, Lukas Faltings, Marc Symons, Julia R. Schneider, Kevin Kwan, John A. Boockvar, Steven S. Gill, J. Miguel Oliveira, Kevin Beccaria, Alexandre Carpentier, Michael Canney, Monica Pearl, Gareth J. Veal, Lisethe Meijer, David A. Walker
Summary: Brain tumors are the leading cause of cancer deaths in children and young adults, but drug treatments have had limited success due to the blood-brain barrier. This review explores techniques to enhance drug delivery to brain tumors and discusses preclinical models for translational development. It also highlights the potential of existing approved drugs when combined with efficient delivery methods. This research aims to address the current challenges faced by children with brain tumors and improve treatment outcomes.
Article
Materials Science, Multidisciplinary
Sunandita Sarker, Adira Colton, Ziteng Wen, Xin Xu, Metecan Erdi, Anthony Jones, Peter Kofinas, Eleonora Tubaldi, Piotr Walczak, Miroslaw Janowski, Yajie Liang, Ryan D. D. Sochol
Summary: This article presents a hybrid additive manufacturing approach combining digital light processing (DLP) 3D printing with ex situ direct laser writing (esDLW) to fabricate new types of microneedle arrays (MNAs) for fluidic microinjections. Experimental results show that the method can create high-density, high-aspect-ratio microneedle arrays with uncompromised fluidic integrity. Ex vivo experiments on mouse brains demonstrate that the MNAs can penetrate and retract from brain tissue and effectively perform microinjections. Overall, this fabrication strategy shows great promise for biomedical microinjection applications.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Pharmacology & Pharmacy
Jing Liu, Chengyan Chu, Jia Zhang, Chongxue Bie, Lin Chen, Safiya Aafreen, Jiadi Xu, David O. Kamson, Peter C. M. van Zijl, Piotr Walczak, Miroslaw Janowski, Guanshu Liu
Summary: The purpose of this study was to develop a label-free MRI approach using CEST technology to detect intracranial accumulation of mannitol following OBBBO. The results showed that CEST MRI could effectively detect mannitol in the brain and may be used to optimize the dosing of mannitol-based OBBBO to improve its safety and efficacy.
Article
Chemistry, Multidisciplinary
Swati Tanwar, Lintong Wu, Noah Zahn, Piyush Raj, Behnaz Ghaemi, Arnab Chatterjee, JeffW. M. Bulte, Ishan Barman
Summary: Quantitative phase imaging (QPI) is a powerful optical imaging technique for label-free, rapid, and three-dimensional (3D) monitoring of cells and tissues. However, there is still limited exploration in using QPI for molecular imaging of intracellular biomolecules such as enzymes. In this study, we present a novel approach by developing QPI contrast agents that enable sensitive detection of intracellular biomolecules. We introduce a new class of bio-orthogonal QPI-nanoprobes for high-contrast refractive index (RI) imaging of enzyme activity, which can potentially have significant implications for disease diagnosis and therapeutic efficacy evaluation.
Article
Neurosciences
Michal Zawadzki, Milosz Pinkiewicz, Mateusz Pinkiewicz, Jerzy Walecki, Piotr Walczak, Dominika Golubczyk, Maria Sady, Zdzislaw Gajewski
Summary: Radiosurgery and endovascular embolization are increasingly preferred over surgical resection for selected cases of brain arteriovenous malformations. Real-time MRI guidance with Onyx shows promising results in monitoring the propagation of the embolic agent and may improve its monitoring in future clinical applications.
Article
Biotechnology & Applied Microbiology
Silvia Vieira, Paulina Strymecka, Luiza Stanaszek, Joana Silva-Correia, Katarzyna Drela, Michal Fiedorowicz, Izabela Malysz-Cymborska, Miroslaw Janowski, Rui Luis Reis, Barbara Lukomska, Piotr Walczak, Joaquim Miguel Oliveira
Summary: This study aims to develop a stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. By supplementing GG-MA solutions with paramagnetic Mn2+ ions, the hydrogel can be visualized under Magnetic Resonance Imaging (MRI). Cell-laden hydrogels prepared using the Mn/GG-MA formulations remained viable after 7 days of culture. In vivo tests on immunocompromised mice showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel visible on MRI scans. Overall, the developed formulations are suitable for non-invasive cell delivery techniques and image-guided neurointerventions.
BIOENGINEERING-BASEL
(2023)
Editorial Material
Nanoscience & Nanotechnology
Jeff W. M. Bulte, Ali Shakeri-Zadeh
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
