Article
Cell Biology
Maria Jassinskaja, Kristyna Pimkova, Nejc Arh, Emil Johansson, Mina Davoudi, Carlos-Filipe Pereira, Ewa Sitnicka, Jenny Hansson
Summary: This study used mass spectrometry and functional assays to investigate the proteomic mechanisms regulating hematopoiesis, revealing distinct features and differentiation capacities between fetal and adult lympho-myeloid multipotent progenitors and granulocyte-monocyte progenitors. The results suggest a specific requirement of myosin activity for myelopoiesis in adult multipotent progenitors and an ontogenic shift in monocytic differentiation capacity of granulocyte-monocyte progenitors driven by differential expression of Irf8.
Review
Biochemistry & Molecular Biology
Mikhail Menshikov, Ekaterina Zubkova, Iuri Stafeev, Yelena Parfyonova
Summary: Mesenchymal stem cells are multipotent cells capable of differentiating into various cell types, and autophagy plays a crucial role in cell differentiation by maintaining cell homeostasis and facilitating phenotype changes.
Article
Cell & Tissue Engineering
Xueyue Wang, Yan Ruan, Junlei Zhang, Yanping Tian, Lianlian Liu, JiaLi Wang, Gaoke Liu, Yuda Cheng, Yixiao Xu, Yi Yang, Meng Yu, Binyu Zhao, Yue Zhang, Jiaqi Wang, Jiangjun Wang, Wei Wu, Ping He, Lan Xiao, Jiaxiang Xiong, Rui Jian
Summary: The study identifies two Yap splicing isoforms in ESCs with different distributions and effects on self-renewal and differentiation potential, with Yap472 playing a more essential role in neuroectoderm differentiation.
Article
Cell Biology
James S. Chavez, Jennifer L. Rabe, Katia E. Nino, Harrison H. Wells, Rachel L. Gessner, Taylor S. Mills, Giovanny Hernandez, Eric M. Pietras
Summary: Chronic inflammation, seen in aging and diseases such as diabetes and obesity, can lead to hematological malignancy. In this study, the role of the transcription factor PU.1 in regulating hematopoietic activity under chronic inflammation was investigated using a PU.1-deficient mouse model. The results showed that PU.1 is critical in restraining inflammatory myelopoiesis and suppressing cell cycle and self-renewal gene programs in myeloid-biased multipotent progenitor (MPP) cells. These findings highlight the importance of PU.1 in regulating emergency myelopoiesis and its relevance to inflammatory diseases and leukemogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
Courtney Chambers, Katerina Cermakova, Yuen San Chan, Kristen Kurtz, Katharina Wohlan, Andrew Henry Lewis, Christiana Wang, Anh Pham, Milan Dejmek, Michal Sala, Mario Loeza Cabrera, Rogelio Aguilar, Radim Nencka, H. Daniel Lacorazza, Rachel E. Rau, H. Courtney Hodges
Summary: In acute myeloid leukemia (AML), SWI/SNF chromatin remodeling complexes sustain leukemic identity by driving high levels of MYC. PU.1 binds to most of its targets in a SWI/SNF-independent manner and recruits SWI/SNF to promote accessibility for other AML core regulatory factors. SWI/SNF inhibition induces therapeutic response in AML but also impairs PU.1-dependent B-cell and monocyte populations, revealing the on-and off-tumor effects of SWI/SNF blockade.
Article
Cell & Tissue Engineering
Zhengqi Wang, Grace Emmel, Hong Seo Lim, Wandi Zhu, Astrid Kosters, Eliver E. B. Ghosn, Peng Qiu, Kevin D. Bunting
Summary: This study reveals the important role of STAT5ab gene in both hematopoietic and stromal cells, and the deletion of STAT5ab using specific Cre promoters leads to a reduction in multipotent hematopoietic progenitors. Furthermore, STAT5ab is involved in the secretion of niche factors in mesenchymal stem cells and the differentiation priming of hematopoietic stem cells.
Article
Cell Biology
Jun Xia, Mengyao Liu, Caiying Zhu, Shicheng Liu, Lanlan Ai, Dongyuan Ma, Ping Zhu, Lu Wang, Feng Liu
Summary: Using single-cell multi-omics, lineage tracing and functional assays, it was discovered that hematopoietic stem and progenitor cells (HSPCs) originate from heterogeneous hemogenic endothelial cells (HECs) during zebrafish embryogenesis. The study revealed the importance of Spi2 in the endothelial-to-hematopoietic transition process and identified molecular determinants of HSPC heterogeneity. These findings provide a foundation for new strategies for inducing lineage-primed HSPCs for transplantation in vitro.
Editorial Material
Cell Biology
Ellen V. Rothenberg
Summary: Transcription factors bind to DNA in a sequence-specific manner and selectively impact gene expression, but their binding sites do not accurately predict the genes they directly control. A new study demonstrates that the same transcription factor binding sites have a greater impact on gene regulation during developmental change.
GENES & DEVELOPMENT
(2022)
Review
Cell Biology
Zoya Mann, Manisha Sengar, Yogesh Kumar Verma, Raja Rajalingam, Pawan Kumar Raghav
Summary: Hematopoietic stem cells possess the properties of self-renewal and differentiation, which can be used for treating various hematological disorders. However, the self-renewal capacity of HSCs decreases with increasing passages, so more research on ex vivo expansion is needed to enhance their self-renewal ability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell & Tissue Engineering
Pijus K. Barman, Helen S. Goodridge
Summary: Balanced production of immune cells is important for immune surveillance, and commensal microbes can influence hematopoietic stem and progenitor cell (HSPC) activity. HSPCs sense microbes through pattern recognition receptors and cytokine receptors, and the long-term effects of microbial stimuli on HSPCs are significant. There may be a connection between myeloid-biased hematopoiesis and elevated levels of microbiome-derived components in aging and metabolic stress. Trained immunity-based vaccines that exploit microbial stimulation of HSPCs show promise.
Article
Biochemical Research Methods
Joanna Handzlik, Manu
Summary: The study reveals that the overall structure of the gene network involved in blood cell development is densely interconnected rather than hierarchical, and the differentiation of cell types is determined by a complex network of genes. The study demonstrates the importance of combining high-resolution time series data with machine learning approaches to infer the structure and causality of gene regulatory networks.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Review
Oncology
Adhiraj Roy, Shivi Chauhan, Sujata Bhattacharya, Vibhuti Jakhmola, Komal Tyagi, Abha Sachdeva, Abdul Wasai, Supratim Mandal
Summary: Cancer is a deadly pathology with millions of new cases and deaths worldwide. Despite advanced therapies, cancer remains fatal due to lack of early prognostic biomarkers, therapy resistance, and the need for novel drug targets.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Immunology
Yelim Kim, Youngyoon Lee, Mi Nam Lee, Jiyeon Nah, Narae Yun, Dayong Wu, Munkyong Pae
Summary: This study found that time-restricted feeding (TRF) can maintain immune cell homeostasis in bone marrow and circulation during obesity, thereby improving metabolic parameters associated with obesity. This suggests that TRF may play a role in regulating immune cells and provides a mechanism for the health benefits associated with TRF.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ruben de Dios, Eduardo Santero, Francisca Reyes-Ramirez
Summary: The bacterial core RNA polymerase can interact with different sigma factors to form a variety of holoenzymes, aiding in coordinating gene expression. Extracytoplasmic function sigma factors (ECFs) play a role in stress responses and have been classified into 157 groups based on their phylogenetic relationships and genomic context, showcasing their diversity. This study focuses on 55 experimentally studied ECF groups, categorizing them into two broad classes of stress responses, and discusses the mechanisms controlling their production and activity for a functional stress response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
B. Edginton-White, A. Maytum, S. G. Kellaway, D. K. Goode, P. Keane, I. Pagnuco, S. A. Assi, L. Ames, M. Clarke, P. N. Cockerill, B. Gottgens, J. B. Cazier, C. Bonifer
Summary: The study reveals that blood cell-specific gene expression is regulated by thousands of differentiation stage-specific cis-elements that respond to cytokine signals terminating at signaling responsive transcription factors. This is an important finding for understanding the mechanisms of hematopoietic differentiation. The authors also describe a high-throughput method to identify differentially active cis-elements in hematopoietic progenitor development, which can be applied to other cell types derived from embryonic stem cells.
NATURE COMMUNICATIONS
(2023)