Article
Neurosciences
Stacey A. Sakowski, Kevin S. Chen
Summary: Stem cell therapy shows promise in treating neurological disorders by providing neuroprotection through metabolic signaling. This interaction includes normalization of glucose metabolism, induction of protective mitochondrial genes, and interactions with neurovasculature. Local metabolic conditions and epigenetic changes in donor cells can affect stem cell biology and transplant viability. Preconditioning stem cells metabolically before transplant can improve their survival. Understanding the metabolic cross-talk between stem cells and the nervous system environment is crucial for successful translation of stem cell therapy in neurological diseases.
NEUROBIOLOGY OF DISEASE
(2022)
Review
Multidisciplinary Sciences
R. Diposarosa, N. A. Bustam, Edhyana Sahiratmadja, P. S. Susanto, Y. Sribudiani
Summary: Hirschsprung's disease is a developmental disorder of the enteric nervous system derived from neural crest cells, and changes in gene expression may contribute to its pathogenesis. Deviant genetic and epigenetic patterns, such as DNA methylation, histone modification, and miRNA regulation, can play a role in the development of neurocristopathy. Specifically, epigenetic regulation of miRNA expression and its relationship to cellular interactions and gene activation through major pathways are discussed in the context of Hirschsprung's disease.
Article
Cell & Tissue Engineering
Rhian Stavely, Sukhada Bhave, Wing Lam N. Ho, Minhal Ahmed, Weikang Pan, Ahmed A. Rahman, Jessica Ulloa, Nicole Bousquet, Meredith Omer, Richard Guyer, Nandor Nagy, Allan M. Goldstein, Ryo Hotta
Summary: The interaction between ENSCs and EMCs plays a crucial role in the normal development of the enteric nervous system, with potential implications for treating Hirschsprung disease. EMCs enhance the expansion and neurosphere-forming ability of ENCDCs through paracrine factors, leading to functional neurons and successful transplantation in the HSCR model.
Article
Biology
Evelien Eenjes, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Gabriela G. Edel, Floor Benthem, Lisette de Kreij-de Bruin, Marco Schnater, Dick Tibboel, Jennifer Collins, Robbert J. Rottier
Summary: SOX21 patterns the airway epithelium by antagonizing SOX2, regulating differentiation of airway progenitor cells. It inhibits basal to ciliated cell differentiation in both development and adult tracheal epithelium. The interplay between SOX2 and SOX21 is context and concentration dependent, crucial for regulating differentiation of the airway epithelium.
Article
Cell & Tissue Engineering
Lisa M. McGinley, Matthew S. Willsey, Osama N. Kashlan, Kevin S. Chen, John M. Hayes, Ingrid L. Bergin, Shayna N. Mason, Aaron W. Stebbins, Jacquelin F. Kwentus, Crystal Pacut, Jennifer Kollmer, Stacey A. Sakowski, Caleb B. Bell, Cynthia A. Chestek, Geoffrey G. Murphy, Parag G. Patil, Eva L. Feldman
Summary: Research evaluated a novel MRI contrast agent for tracking stem cells post intracranial transplantation in vivo. Results demonstrated that the contrast agent does not impact cell viability and provides robust MRI contrast in animal brains. Linear correlations suggest the contrast agent aids in determining graft survival or rejection.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Sabine Heumuller-Klug, Kristina Maurer, Maria A. Tapia-Laliena, Carsten Sticht, Anne Christmann, Handan Moerz, Rasul Khasanov, Elvira Wink, Steven Schulte, Wolfgang Greffrath, Rolf-Detlef Treede, Lucas M. Wessel, Karl-Herbert Schaefer
Summary: Impairment of the nervous system is a major cause of mortality and disability, and congenital enteric dysganglionosis requires neural stem cell transplantation for treatment. This study investigates the impact of freezing and thawing protocols on enteric neural stem cell survival, protein and gene expression, and cell function. The results show that cryopreservation of enteric neural stem cells is possible with minimal changes to protein/gene expression patterns and without affecting neuronal function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yue Ji, Paul Kwong-Hang Tam, Clara Sze-Man Tang
Summary: The development of the enteric nervous system relies on the interaction between enteric neural crest cells and the neural stem cell niche. Defects in ENCCs may lead to ENS dysfunction. HSCR patients may have niche defects affecting ECM-cell interaction, suggesting the need for complementary therapies involving drugs and niche-based treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Nicholas E. Stone, Andrew P. Voigt, Robert F. Mullins, Todd Sulchek, Budd A. Tucker
Summary: Autologous photoreceptor cell replacement is a promising approach for treating inherited retinal degenerative blindness, but enrichment of photoreceptor cells and correction of disease-causing genetic mutations are crucial. While advanced retinal differentiation protocols can generate various cell types, most of them are unnecessary and may hinder successful transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Makoto Ohmoto, Shugo Nakamura, Hong Wang, Peihua Jiang, Junji Hirota, Ichiro Matsumoto
Summary: This study reveals the dynamics of single stem cells in the turnover of taste bud cells. The Sox2(+) stem cell population is maintained by balancing the increase of certain stem cells with the reduction of others. Taste buds are composed of cells derived from multiple Sox2(+) stem cells.
Article
Pediatrics
Tingting Zhou, Wei Liu, Xiaofang Yu, Zengcai Cao, Weijing Mu, Peimin Hou, Chuantao Ren, Aiwu Li
Summary: Aberrant development of myenteric EGCs was observed in the colon of HSCR, potentially affecting the survival of enteric neurons. Protein and mRNA expression of glial fibrillary acidic protein and S100 calcium-binding protein B showed a decreasing trend from proximal, dilated, and transitional segments to the aganglionic segment.
FRONTIERS IN PEDIATRICS
(2021)
Article
Cell & Tissue Engineering
Mingliang Bai, Guoping Li, Zeyidan Jiapaer, Xudong Guo, Jiajie Xi, Guiying Wang, Dan Ye, Wen Chen, Baoyu Duan, Jiuhong Kang
Summary: This study identifies a long non-coding RNA (linc1548) as a critical regulator of neural differentiation in mouse embryonic stem cells (mESCs). Linc1548 interacts with transcription factors OCT6 and SOX2 to form an RNA-protein complex, controlling the transition from epiblast stem cells (EpiSCs) to neural progenitor cells (NPCs). The findings provide insight into the intrinsic mechanism of neural fate determination.
Article
Pharmacology & Pharmacy
Masato Maruyama, Minami Yoshikata, Mana Sakaguchi, Shizuka Wakushima, Kazutaka Higaki
Summary: The role of the enteric nervous system (ENS) in drug absorption is not well understood. In this study, a co-culture system of Caco-2 cells and enteric neurons was established to investigate the effect of ENS on drug transport across intestinal epithelial cells. The co-culture was found to enhance the transport of fluorescein isothiocyanate-labeled dextran-4 via the paracellular route, suggesting that enteric neurons can facilitate drug transport by opening tight junctions. However, no changes in the permeability of antipyrine were observed, indicating that enteric neurons do not affect passive transport via the transcellular pathway.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Biochemistry & Molecular Biology
Richard A. Guyer, Jessica L. Mueller, Allan M. Goldstein
Summary: With the advancement of technology, single-cell sequencing has become a common tool to analyze cellular diversity and reveal cellular diversity within organs. The application of these methods in the study of the enteric nervous system has provided abundant data and biological insights. As technology continues to evolve, these methods may become commonplace in enteric nervous system research and diagnostic testing.
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Tinh Thi Nguyen, Peter Baumann, Oliver Tuescher, Sandra Schick, Kristina Endres
Summary: The gut and the brain communicate through various channels and interactions, collectively known as the gut-brain axis. The aging of the enteric nervous system (ENS) in the gut can lead to gastrointestinal dysfunction and contribute to age-related diseases of the brain. Understanding the mechanisms of the aging ENS can provide insights into the diagnosis and treatment of neurodegenerative diseases like Alzheimer's and Parkinson's.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Chrysoula Konstantinidou, Stavros Taraviras, Vassilis Pachnis
Article
Developmental Biology
Tiffany A. Heanue, Iain T. Shepherd, Alan J. Burns
DEVELOPMENTAL BIOLOGY
(2016)
Article
Genetics & Heredity
Tiffany A. Heanue, Werend Boesmans, Donald M. Bell, Koichi Kawakami, Pieter Vanden Berghe, Vassilis Pachnis
Correction
Multidisciplinary Sciences
Kai Liu, Juhyun Kim, Dong Won Kim, Yi Stephanie Zhang, Hechen Bao, Myrto Denaxa, Szu-Aun Lim, Eileen Kim, Chang Liu, Ian R. Wickersham, Vassilis Pachnis, Samer Hattar, Juan Song, Solange P. Brown, Seth Blackshaw
Article
Multidisciplinary Sciences
Kai Liu, Juhyun Kim, Dong Won Kim, Yi Stephanie Zhang, Hechen Bao, Myrto Denaxa, Szu-Aun Lim, Eileen Kim, Chang Liu, Ian R. Wickersham, Vassilis Pachinis, Samer Hattar, Juan Song, Solange P. Brown, Seth Blackshaw
Article
Multidisciplinary Sciences
Reena Lasrado, Werend Boesmans, Jens Kleinjung, Carmen Pin, Donald Bell, Leena Bhaw, Sarah McCallum, Hui Zong, Liqun Luo, Hans Clevers, Pieter Vanden Berghe, Vassilis Pachnis
Article
Gastroenterology & Hepatology
Fatima Memic, Viktoria Knoflach, Khomgrit Morarach, Rebecca Sadler, Catia Laranjeira, Jens Hjerling-Leffler, Erik Sundstrom, Vassilis Pachnis, Ulrika Marklund
Article
Cell Biology
Myrto Denaxa, Guilherme Neves, Adam Rabinowitz, Sarah Kemlo, Petros Liodis, Juan Burrone, Vassilis Pachnis
Article
Neurosciences
Werend Boesmans, Marlene M. Hao, Candice Fung, Zhiling Li, Chris Van den Haute, Jan Tack, Vassilis Pachnis, Pieter Vanden Berghe
Article
Multidisciplinary Sciences
Myrto Denaxa, Guilherme Neves, Juan Burrone, Vassilis Pachnis
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Multidisciplinary Sciences
Yuuki Obata, Alvaro Castano, Stefan Boeing, Ana Carina Bon-Frauches, Candice Fung, Todd Fallesen, Mercedes Gomez De Aguero, Bahtiyar Yilmaz, Rita Lopes, Almaz Huseynova, Stuart Horswell, Muralidhara Rao Maradana, Werend Boesmans, Pieter Vanden Berghe, Andrew J. Murray, Brigitta Stockinger, Andrew J. Macpherson, Vassilis Pachnis
Article
Pharmacology & Pharmacy
Song Hui Chng, Vassilis Pachnis
CURRENT OPINION IN PHARMACOLOGY
(2020)
Article
Biology
Sarah McCallum, Yuuki Obata, Evangelia Fourli, Stefan Boeing, Christopher J. Peddie, Qiling Xu, Stuart Horswell, Robert N. Kelsh, Lucy Collinson, David Wilkinson, Carmen Pin, Vassilis Pachnis, Tiffany A. Heanue
Article
Neurosciences
Myrto Denaxa, Guilherme Neves, Juan Burrone, Vassilis Pachnis
JOURNAL OF EXPERIMENTAL NEUROSCIENCE
(2018)
Review
Immunology
Henrique Veiga-Fernandes, Vassilis Pachnis