Article
Biochemistry & Molecular Biology
Aiguo Tian, Virginia Morejon, Sarah Kohoutek, Yi-Chun Huang, Wu-Min Deng, Jin Jiang
Summary: In the Drosophila intestine, infection with pathogenic bacteria induces enteroblasts (ISC progenies) to enter the mitotic cycle through upregulation of the epidermal growth factor receptor (EGFR)-Ras signaling pathway. The regenerative ISCs are produced through mitosis of enteroblasts, which do not gain ISC identity before dividing.
Article
Hematology
Valentina Capo, Sara Penna, Ivan Merelli, Matteo Barcella, Serena Scala, Luca Basso-Ricci, Elena Draghici, Eleonora Palagano, Erika Zonari, Giacomo Desantis, Paolo Uva, Roberto Cusano, Lucia Sergi Sergi, Laura Crisafulli, Despina Moshous, Polina Stepensky, Katarzyna Drabko, Zuhre Kaya, Ekrem Unal, Alper Gezdirici, Giuseppe Menna, Marta Serafini, Alessandro Aiuti, Silvia Laura Locatelli, Carmelo Carlo-Stella, Ansgar S. Schulz, Francesca Ficara, Cristina Sobacchi, Bernhard Gentner, Anna Villa
Summary: Allogeneic hematopoietic stem cell transplantation is the preferred treatment for autosomal recessive osteopetrosis caused by TCIRG1 gene defects. However, some patients are not eligible due to disease severity. Utilizing circulating CD34(+) cells for gene therapy shows promise in overcoming limitations of stem cell harvest in osteopetrotic patients, potentially shaping future gene-based treatments for skeletal diseases.
Review
Immunology
Mariona Baliu-Pique, Kiki Tesselaar, Jose A. M. Borghans
Summary: Timely recovery of T-cell numbers following HSCT is crucial for preventing complications. T-cell dynamics in humans are thought to be regulated in a cell density-dependent manner. However, T-cell reconstitution after HSCT is notoriously slow, raising questions about the existence of homeostatic mechanisms in humans.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Adam M. Passman, Magnus J. Haughey, Emanuela Carlotti, Marc J. Williams, Bianca Cereser, Meng-Lay Lin, Shruthi Devkumar, Jonathan P. Gabriel, Enrico Gringeri, Umberto Cillo, Francesco Paolo Russo, Matthew Hoare, Joanne ChinAleong, Marnix Jansen, Nicholas A. Wright, Hermant M. Kocher, Weini Huang, Malcolm R. Alison, Stuart A. C. McDonald
Summary: The origin and expansion dynamics of hepatocyte clones in the normal human liver have been determined using lineage tracing, methylation sequence analysis, and next-generation sequencing. Clonal patches of hepatocytes commonly associate with portal tracts and can lineage-trace with cholangiocytes, indicating a common ancestor at this niche. The patterns of mitochondrial DNA variants reveal spatially restricted mutations and limited clonal mutations, indicating slow growth and quiescence of clonal patches.
JOURNAL OF HEPATOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zhiwei Yin, Rui Shi, Xin Xia, Ling Li, Yanxia Yang, Shengkai Li, Jieqiong Xu, Yiting Xu, Xinqi Cai, Shen Wang, Zhangkun Liu, Tianhuan Peng, Ying Peng, Hua Wang, Mao Ye, Yanlan Liu, Zhuo Chen, Weihong Tan
Summary: This article presents a vascular-like integrated trapped device (ITD) with adhesive sites and wireless magnetothermal response for removing highly metastatic tumor cells. The device shows good performance in capture efficiency and cell death, and it can prevent vascular blockage and induce potential vascular regeneration.
ADVANCED MATERIALS
(2022)
Article
Developmental Biology
Guihua Du, Melissa J. Oatley, Nathan C. Law, Colton Robbins, Xin Wu, Jon M. Oatley
Summary: During development, the undifferentiated spermatogonial population in mammals plays a crucial role during a quiescence period, with disruptions in mitotic arrest leading to apoptosis in prospermatogonia and loss of germ cells. Single-cell RNA-sequencing analysis reveals that oxidative phosphorylation activity and meiotic initiation are affected in prospermatogonia without a normal quiescence period, suggesting that key programming layers are established during this period for proper fate determination and postnatal fitness.
Article
Multidisciplinary Sciences
Keiyo Takubo, Phyo Wai Htun, Takeshi Ueda, Yasuyuki Sera, Masayuki Iwasaki, Miho Koizumi, Kohei Shiroshita, Hiroshi Kobayashi, Miho Haraguchi, Shintaro Watanuki, Zen-ichiro Honda, Norimasa Yamasaki, Ayako Nakamura- Ishizu, Fumio Arai, Noboru Motoyama, Tomohisa Hatta, Tohru Natsume, Toshio Suda, Hiroaki Honda
Summary: The gene Mbtd1 plays a crucial role in regulating the number and function of hematopoietic stem cells. In mice deficient in Mbtd1, the numbers of stem cells and progenitors increase, and these deficient stem cells exhibit an overactive cell cycle and impaired response to stress. The study also shows that Mbtd1 is directly involved in maintaining cell cycle quiescence by binding to the promoter region of the FoxO3a gene, which is essential for the normal function of hematopoietic stem cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Kei-ichiro Arimoto, Sayuri Miyauchi, Ty D. Troutman, Yue Zhang, Mengdan Liu, Samuel A. Stoner, Amanda G. Davis, Jun-Bao Fan, Yi-Jou Huang, Ming Yan, Christopher K. Glass, Dong-Er Zhang
Summary: Immunotherapy is an effective cancer treatment, but not for all patients, prompting the need for alternative strategies. Inducing cancer immunogenic cell death (ICD) shows promise in promoting robust immune responses against tumor-associated antigens. Depletion of USP18, a negative regulator of interferon signaling, selectively induces ICD in cancer cells, suggesting targeting USP18 as a potential cancer immunotherapy.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Sudan Puri, Isabel Y. Moreno, Mingxia Sun, Sudhir Verma, Xiao Lin, Tarsis F. Gesteira, Vivien J. Coulson-Thomas
Summary: This study found that a hyaluronan (HA) matrix is present in the niche surrounding limbal epithelial stem cells (LESCs) during ex vivo expansion. The presence of both endogenous and exogenous HA was shown to help maintain the LESC phenotype and enhance proliferation, colony formation, and expression of stem cell markers. These findings suggest that HA could be a cost-effective substrate or supplement for culturing LESCs in clinical settings.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Arantza Infante, Leire Cabodevilla, Blanca Gener, Clara I. Rodriguez
Summary: Osteogenesis Imperfecta (OI) is a rare genetic disease characterized by bone fragility, and currently there is no cure. Research on therapeutic approaches such as cell therapy with mesenchymal stem cells (MSCs) and inhibition of the TGF-beta pathway for OI patients has shown promising outcomes. Excessive TGF-beta signaling pathway has been found in OI patients and could be modulated by MSCs therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Zutong Li, Rou Wang, Dandan Wang, Shujie Zhang, Hua Song, Shuai Ding, Yantong Zhu, Xin Wen, Hui Li, Hongwei Chen, Shanshan Liu, Lingyun Sun
Summary: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by autoantibody production and tissue inflammation. Mesenchymal stem cells (MSCs) have emerged as a promising candidate therapy for SLE due to their immunomodulatory and regenerative properties. Recent studies have identified differentially expressed circulating miRNAs in autoimmune diseases, including SLE. In this study, small RNA sequencing analysis was performed to compare the circulating miRNA profiles of SLE patients before and after MSC transplantation, and a significant decrease in circulating miR-320b level during MSCT was identified. Circulating miR-320b and its target gene MAP3K1 were found to be closely associated with SLE disease activity. In vitro experiments showed that decreased MAP3K1 level in SLE peripheral blood mononuclear cells (PBMCs) was involved in CD4+ T-cell proliferation. In a mouse model, miR-320b overexpression aggravated SLE symptoms, while miR-320b inhibition promoted disease remission. Furthermore, MSCs regulated miR-320b/MAP3K1 expression both in vitro and in vivo. Overall, this study suggests that circulating miR-320b and MAP3K1 may play a role in CD4+ T-cell proliferation in SLE.
JOURNAL OF IMMUNOLOGY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Masaru Mizukoshi, Masaru Kaku, Lay Thant, Kohei Kitami, Moe Arai, Isao Saito, Katsumi Uoshima
Summary: The study revealed that periodontal ligament (PDL) cells exhibit dynamic proliferative characteristics during orthodontic tooth movement-induced tissue remodeling, with proliferative cells likely undergoing further division and contributing to tissue remodeling. Additionally, proliferating cells expressed various molecular markers, indicating the lineage plasticity of PDL cells in vivo.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Yong-Hong Wang, Ya-Chao Tao, Dong-Bo Wu, Meng-Lan Wang, Hong Tang, En-Qiang Chen
Summary: Different storage solutions did not significantly affect cell behavior, while heterogeneity was prevalent in MSC populations and could limit cell application. Therefore, establishing a more precise standard for culture-expanded MSCs is necessary.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Junchen Chen, Xiaoxue Dong, Xuejun Cheng, Qiang Zhu, Jinyu Zhang, Qian Li, Xiaoli Huang, Min Wang, Liping Li, Weixiang Guo, Binggui Sun, Qiang Shu, Wen Yi, Xuekun Li
Summary: Ogt deficiency in adult neural stem/progenitor cells leads to a decrease in the aNSPC pool, abnormal neurogenesis, and impaired cognitive function in adult mice. Mechanistic studies reveal that Ogt catalyzes the O-GlcNAc modification of the Notch TM/ICD fragment, which in turn affects neurogenic defects induced by Ogt deficiency.
Article
Biochemistry & Molecular Biology
Tania Velletri, Carlo Emanuele Villa, Domenica Cilli, Bianca Barzaghi, Pietro Lo Riso, Michela Lupia, Raffaele Luongo, Alejandro Lopez-Tobon, Marco De Simone, Raoul J. P. Bonnal, Luca Marelli, Stefano Piccolo, Nicoletta Colombo, Massimiliano Pagani, Ugo Cavallaro, Saverio Minucci, Giuseppe Testa
Summary: This study introduces a new method for isolating and growing single cells from patients' metastatic ascites to establish 3D cultures referred to as sMOCS. By using single cell RNA sequencing, researchers were able to define the cellular composition of metastatic ascites and reproduce features of the original metastasis that cannot be observed in traditional 2D culture. This method provides a powerful tool for precision oncology in ovarian cancer by enriching informative cell subpopulations and analyzing their diversity at the functional and molecular level.
CELL DEATH AND DIFFERENTIATION
(2022)
