Article
Cell & Tissue Engineering
Huangfan Xie, Zhongjie Sun, Xiong Xiao, Defang Liu, Hailong Qi, Guoxiong Tian, Miao Chen, Ligong Chen, Xuncheng Su
Summary: Transient inhibition of the JNK pathway can increase the frequency of HSCs in cord blood and promote their quiescence, leading to the enhancement of HSC number and engraftment potential. These findings have the potential to improve HSC collection and transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Runfeng Miao, Harim Chun, Xing Feng, Ana Cordeiro Gomes, Jungmin Choi, Joao P. Pereira
Summary: This study reveals that a complex homeostatic balance between hematopoietic stem cells (HSCs) and hematopoietic progenitor cells is maintained through competition for a limited amount of cell signaling molecule. When early hematopoietic progenitors fail to interact with specific cells, HSC numbers increase.
NATURE COMMUNICATIONS
(2022)
Review
Hematology
Fernando Anjos-Afonso, Dominique Bonnet
Summary: The ability to isolate and characterize different hematopoietic stem cell (HSC) or progenitor cell populations provides insights into the regulation of hematopoiesis in various conditions. While significant progress has been made in understanding the composition of these cell types in mice, recent breakthroughs have improved the resolution of the human primitive hematopoietic compartment. This review aims to provide a historical perspective and discuss the progress in characterizing human postnatal CD34(+) HSC-enriched populations, with potential implications for future translational studies.
Article
Cell & Tissue Engineering
Sara Bucar, Andre Dargen de Matos Branco, Marcia F. Mata, Joao Coutinho Milhano, Iris Caramalho, Joaquim M. S. Cabral, Ana Fernandes-Platzgummer, Claudia L. da Silva
Summary: The study compared the capacity of MSC from different sources to expand UCB CD34(+) cells ex vivo, with AT-derived MSC showing superior expansion potential and maintenance of primitive cells. UCM-derived MSC demonstrated inferior hematopoietic supportive capacity compared to MSC from adult tissues. Further research is needed to determine the impact of HPL on the hematopoietic supportive capacity of MSC.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Hematology
Yinghui Li, Wenshan Zhang, Yu Zhang, Yahui Ding, Ming Yang, Mei He, Xiaolei Liu, Jiali Gu, Shiqi Xu, Zhiwei Feng, Yafang Li, Jingjing Yin, Huier Gao, Henan Song, Hui Xu, Chaoqun Wang, Qing Ji, Shihui Ma, Wanzhu Yang, Weiping Yuan, Xiang-Qun Xie, Tao Cheng, Yingdai Gao
Summary: The use of umbilical cord blood transplant is limited by the number of hematopoietic stem cells. A small molecule inhibitor of p18, 005A, enhances the self-renewal of long-term HSCs in humans, allowing cells to maintain their functionality in secondary recipients.
Article
Biochemistry & Molecular Biology
Yiqi Yang, Bihui Zhang, Junye Xie, Jingsheng Li, Jia Liu, Rongzhan Liu, Linhao Zhang, Jinting Zhang, Zijian Su, Fu Li, Leisheng Zhang, An Hong, Xiaojia Chen
Summary: The CH02 peptide is effective in ex vivo expansion of CD34(+) umbilical cord blood hematopoietic stem/progenitor cells and promotes wound healing in diabetic mice through bidirectional orchestration of proinflammatory and anti-inflammatory factors.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Cell & Tissue Engineering
Natsumi Miharada, Anna Rydstrom, Justyna Rak, Jonas Larsson
Summary: Genetic deletion of Gata2 allows efficient conditioning of recipient HSCs for transplantation without irradiation, leading to robust engraftment of donor HSCs. Different modes and timings of recipient conditioning can influence the functional features of transplanted HSCs.
Article
Biophysics
Alice Pievani, Valentina Granata, Giacomo Desantis, Laura Antolini, Sara Ornaghi, Antonio Galleu, Andrea Biondi, Bernhard Gentner, Francesco Dazzi, Marta Serafini
Summary: Monocytes are effective at improving engraftment in HSPC transplantation, particularly in cases of limited cell doses such as CB transplantation and HSC gene therapy. Co-culturing monocytes increases survival and stemness of CB-CD34(+) cells. Soluble factors and direct-cell contact interactions play critical roles in the HSC-monocyte crosstalk.
BONE MARROW TRANSPLANTATION
(2022)
Article
Immunology
Sabrina B. Bennstein, Sandra Weinhold, Oezer Degistirici, Robert A. J. Oostendorp, Katharina Raba, Gesine Koegler, Roland Meisel, Lutz Walter, Markus Uhrberg
Summary: ILC3, particularly IL-22-secreting ILC3, play vital roles in maintaining mucosal barrier functions and controlling inflammatory diseases. A method for generating functionally competent human ILC3 from cord blood-derived CD34(+) hematopoietic progenitors on human MSCs has been developed, showing promise for potential clinical applications in treating inflammatory diseases and cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Florian M. Hatzmann, Asim Ejaz, G. Jan Wiegers, Markus Mandl, Camille Brucker, Stefan Lechner, Tina Rauchenwald, Marit Zwierzina, Saphira Baumgarten, Sonja Wagner, Monika Mattesich, Petra Waldegger, Gerhard Pierer, Werner Zwerschke
Summary: This study reveals that DLK1(-)/CD34(+) ASCs in human subcutaneous white adipose tissue exist in a quiescent state, express high levels of somatic stemness factors and the early adipogenic transcription factor C/EBP beta, but senescence and pluripotency markers are barely detectable. CD24 is shown to be necessary for proper ASC proliferation and adipogenesis, and DLK1(-)/CD34(+)/CD24(+) cells exhibit higher stemness and lower adipogenic capacity compared to DLK1(-)/CD34(+)/CD24(-) subpopulations.
Article
Biochemistry & Molecular Biology
Takenobu Nii, Katsuhiro Konno, Masaki Matsumoto, Kanit Bhukhai, Suparerk Borwornpinyo, Kazuhiro Sakai, Suradej Hongeng, Daisuke Sugiyama
Summary: The bioactive peptide SL-13R has been developed to promote the expansion of umbilical cord blood CD34+ cells in vitro, showing significant increases in cell numbers and long-term reconstitution ability. The interaction of SL-13R with specific genes AHNAK, ANXA2, and PLEC suggests potential clinical applications for SL-13R in the future.
Article
Cell Biology
Dongwei Guan, Yonghao Yang, Mao Pang, Xinlei Liu, Yang Li, Pengju Huang, Haitao Shang, Hong Wei, Zhijia Ye
Summary: A study found that Indole-3-carboxaldehyde (I3A), a metabolite derived from gut microbiota, can extend the lifespan of mice exposed to high-dose ionizing radiation. Currently, there are limited therapeutic options for the most common and fatal complication of nuclear accidents and radiotherapy, which is persistent myelosuppression. However, it is still unknown whether and how I3A protects against radiation-induced hematopoietic toxicity.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Kim Vanuytsel, Carlos Villacorta-Martin, Jonathan Lindstrom-Vautrin, Zhe Wang, Wilfredo F. Garcia-Beltran, Vladimir Vrbanac, Dylan Parsons, Evan C. Lam, Taylor M. Matte, Todd W. Dowrey, Sara S. Kumar, Mengze Li, Feiya Wang, Anthony K. Yeung, Gustavo Mostoslavsky, Ruben Dries, Joshua D. Campbell, Anna C. Belkina, Alejandro B. Balazs, George J. Murphy
Summary: This study analyzes the molecular signature of hematopoietic stem cells during engraftment and identifies CD201 as a marker for enriching engraftment potential. It provides important insights for retaining and inducing the engraftment potential of hematopoietic stem cells.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Mao Kondo, Koki Kimura, Jingjing Kobayashi -Sun, Shiori Yamamori, Makoto Taniguchi, David Traver, Isao Kobayashi
Summary: The zebrafish is a unique model for studying hematopoietic niches, and it has been found that sinusoidal endothelium plays a crucial role in the zebrafish kidney. Loss of the junctional adhesion molecule 1a (Jam1a) leads to a reduction in sinusoids and a defect in hematopoietic niches, indicating the importance of Jam1a in the formation of sinusoids and hematopoietic niches in the zebrafish kidney.
Article
Cell & Tissue Engineering
Ying-Ying Chen, Yu-Feng Liu, Yong-Dong Liu, Xiao-Hui Deng, Jie Zhou
Summary: IRF7 is an important regulator of stress hematopoiesis, as its deficiency enhances stem cell regeneration and long-term repopulation under stress conditions. Mechanistic studies reveal CXCR4 as a downstream target of IRF7, with overexpression of CXCR4 abolishing the enhanced proliferation and regeneration observed in IRF7-deficient HSPCs.
Article
Hematology
Kotaro Shide, Takuro Kameda, Ayako Kamiunten, Yoshinori Ozono, Yuki Tahira, Takako Yokomizo-Nakano, Sho Kubota, Masaya Ono, Kazuhiko Ikeda, Masaaki Sekine, Keiichi Akizuki, Kenichi Nakamura, Tomonori Hidaka, Yoko Kubuki, Hisayoshi Iwakiri, Satoru Hasuike, Kenji Nagata, Goro Sashida, Kazuya Shimoda
Article
Oncology
Kazumasa Aoyama, Daisuke Shinoda, Emi Suzuki, Yaeko Nakajima-Takagi, Motohiko Oshima, Shuhei Koide, Ola Rizq, Sha Si, Shiro Tara, Goro Sashida, Atsushi Iwama
Summary: Deletion of Ezh1 and Ezh2 leads to PRC2 insufficiency, causing dyserythropoiesis and activating the p53 pathway by de-repressing the Cdkn2a gene, contributing to disease progression. Rescuing dyserythropoiesis can be achieved by deleting Cdkn2a and p53.
Article
Biochemistry & Molecular Biology
Jie Bai, Takako Yokomizo-Nakano, Sho Kubota, Yuqi Sun, Akinori Kanai, Mihoko Iimori, Hironori Harada, Atsushi Iwama, Goro Sashida
Summary: High Mobility Group AT-hook 2 (HMGA2) is found to be overexpressed in patients with myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), with its expression level finely tuned by Lin28b-Let-7 axis and Polycomb Repressive Complex 2. The overexpression of HMGA2 can activate the oncogene Igf2bp2 and enhance self-renewal of Tet2-deficient stem cells, affecting the transcriptional program and differentiation of myeloid cells. These findings highlight the potential therapeutic target of HMGA2-Igf2bp2 axis in the transformation of stem cells.
Review
Hematology
Takako Yokomizo-Nakano, Goro Sashida
Summary: RUNX3, known as a tumor suppressor in various cancers, has been found to have an oncogenic function in myeloid malignancies. Although deletions and loss-of-function mutations in RUNX3 are rare in hematopoietic malignancies, its overexpression can lead to malignant transformation.
EXPERIMENTAL HEMATOLOGY
(2021)
Article
Cell Biology
Tadahito Yasuda, Mayu Koiwa, Atsuko Yonemura, Keisuke Miyake, Ryusho Kariya, Sho Kubota, Takako Yokomizo-Nakano, Noriko Yasuda-Yoshihara, Tomoyuki Uchihara, Rumi Itoyama, Luke Bu, Lingfeng Fu, Kota Arima, Daisuke Izumi, Shiro Iwagami, Kojiro Eto, Masaaki Iwatsuki, Yoshifumi Baba, Naoya Yoshida, Hiroto Ohguchi, Seiji Okada, Keisuke Matsusaki, Goro Sashida, Akiko Takahashi, Patrick Tan, Hideo Baba, Takatsugu Ishimoto
Summary: In the tumor microenvironment, senescent cancer-associated fibroblasts (CAFs) play a role in promoting gastric cancer (GC) peritoneal tumor formation through maintenance of the senescence-associated secretory phenotype (SASP) and JAK/STAT3 signaling. Single-cell mass cytometry analysis revealed a high expression level of senescent fibroblasts and SASP factors in the ascites of GC patients. These findings provide insights into the molecular mechanisms of inflammation-related SASP maintenance and the involvement of senescent CAFs in GC peritoneal dissemination.
Review
Oncology
Kanlayanee Sawanyawisuth, Goro Sashida, Guojun Sheng
Summary: This review summarizes the mechanisms of parasitic infection in the pathogenesis of bile duct cancer, focusing on the effects of epithelial-mesenchymal transition (EMT) in bile duct epithelial cells and its association with epigenetic dysregulation. It suggests that both direct and indirect mechanisms underlie the parasitic infection-induced EMT in cholangiocarcinoma (CCA).
Article
Oncology
Daisuke Shinoda, Yaeko Nakajima-Takagi, Motohiko Oshima, Shuhei Koide, Kazumasa Aoyama, Atsunori Saraya, Hironori Harada, Bahityar Rahmutulla, Atsushi Kaneda, Kiyoshi Yamaguchi, Yoichi Furukawa, Haruhiko Koseki, Kazuya Shimoda, Tomoaki Tanaka, Goro Sashida, Atsushi Iwama
Summary: The insufficiency of PRC1.1, a component of polycomb repressive complex 1, promotes the development of myelofibrosis and acts as a tumor suppressor in this process. This impact is distinct from the effect of PRC2 insufficiency on the pathogenesis of myelofibrosis.
Letter
Oncology
Mohamed Gaber Abdallah, Akiko Niibori-Nambu, Mariko Morii, Takako Yokomizo, Tomomasa Yokomizo, Takako Ideue, Sho Kubota, Vania Swee Imm Teoh, Michelle Meng Huang Mok, Chelsia Qiuxia Wang, Abdellah Ali Omar, Kenji Tokunaga, Eisaku Iwanaga, Masao Matsuoka, Norio Asou, Naomi Nakagata, Kimi Araki, Mabrouk AboElenin, Sayed Hamada Madboly, Goro Sashida, Motomi Osato
Article
Hematology
Yuqi Sun, Sho Kubota, Mihoko Iimori, Ai Hamashima, Haruka Murakami, Jie Bai, Mariko Morii, Takako Yokomizo-Nakano, Motomi Osato, Kimi Araki, Goro Sashida
Summary: This study reveals the critical role of the acidic domain and linker region of Hmga2 in modulating the transcription and self-renewal functions of hematopoietic stem cells.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Terumasa Umemoto, Alban Johansson, Shah Adil Ishtiyaq Ahmad, Michihiro Hashimoto, Sho Kubota, Kenta Kikuchi, Haruki Odaka, Takumi Era, Daisuke Kurotaki, Goro Sashida, Toshio Suda
Summary: By studying the mitochondrial metabolism and ATP citrate lyase (ACLY) in hematopoietic stem cells (HSCs), we have gained new insights into the regulation of HSCs during hematopoietic regeneration.
Article
Biochemistry & Molecular Biology
Supannika Sorin, Sho Kubota, Sofiane Hamidi, Takako Yokomizo-Nakano, Kulthida Vaeteewoottacharn, Sopit Wongkham, Sakda Waraasawapati, Chawalit Pairojkul, Jie Bai, Mariko Morii, Guojun Sheng, Kanlayanee Sawanyawisuth, Goro Sashida
Summary: This study found that the high expression of high mobility group nucleosome-binding protein 3 (HMGN3) is correlated with the metastasis of cholangiocarcinoma (CCA). HMGN3 inhibits the expression of epithelial regulator genes by binding with the transcription factor SNAI2 and regulating histone deacetylases (HDACs), thereby affecting the migration capacity of CCA cells.
Article
Biology
Takuro Kameda, Kotaro Shide, Ayako Kamiunten, Yasunori Kogure, Daisuke Morishita, Junji Koya, Yuki Tahira, Keiichi Akizuki, Takako Yokomizo-Nakano, Sho Kubota, Kosuke Marutsuka, Masaaki Sekine, Tomonori Hidaka, Yoko Kubuki, Yuichi Kitai, Tadashi Matsuda, Akinori Yoda, Takayuki Ohshima, Midori Sugiyama, Goro Sashida, Keisuke Kataoka, Seishi Ogawa, Kazuya Shimoda
Summary: The study investigates the oncogenic ability of mutant CARD11 (E626K), one of the most frequently mutated genes in adult T-cell leukemia, and its cooperative effect with HBZ expression. Using a mouse model, the researchers clarify the pathogenetic effects of these genes and find that they cooperatively activate multiple signaling pathways, promoting the development of ATL.
COMMUNICATIONS BIOLOGY
(2022)
Article
Oncology
Shingo Kawano, Kimi Araki, Jie Bai, Imari Furukawa, Keigo Tateishi, Kumiko Yoshinobu, Shingo Usuki, Rachael A. Nimmo, Tadashi Kaname, Masaharu Yoshihara, Satoru Takahashi, Goro Sashida, Masatake Araki
Summary: miR-142 mutation plays a crucial role in blood cancers, causing a loss of function in half of the wild-type miR-142-3p alleles and gaining the function to suppress the expression of specific target genes, sufficient to induce leukemogenesis in mice.
Article
Immunology
Takako Yokomizo-Nakano, Ai Hamashima, Sho Kubota, Jie Bai, Supannika Sorin, Yuqi Sun, Kenta Kikuchi, Mihoko Iimori, Mariko Morii, Akinori Kanai, Atsushi Iwama, Gang Huang, Daisuke Kurotaki, Hitoshi Takizawa, Hirotaka Matsui, Goro Sashida
Summary: Prior infection stress and loss of Tet2 gene can remodel the transcriptional and epigenetic landscapes and cellular functions in hematopoietic stem cells (HSCs) via the Trif-Plk-Elf1 axis and drive the development of myelodysplastic syndrome (MDS). Pharmacological inhibition of Plk function or knockdown of Elf1 expression can prevent epigenetic remodeling in HSCs and alleviate clonogenicity enhancement and impaired erythropoiesis. Additionally, the Elf1-target signature is significantly enriched in MDS HSPCs in humans.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Supannika Sorin, Nongnapas Pokaew, Kulthida Vaeteewoottacharn, Sakda Waraasawapati, Chawalit Pairojkul, Goro Sashida, Kanlayanee Sawanyawisuth
Summary: This study investigated the expression of HMGN proteins in cholangiocarcinoma (CCA) and found that HMGN3 is abnormally expressed in CCA tissues. Further analysis revealed a correlation between high HMGN3 expression and tumor invasion in CCA patients, as well as a positive correlation between HMGN3 and transforming growth factor-β expression in CCA tissues.
