Review
Biochemistry & Molecular Biology
Maria G. Detsika, Elias A. Lianos
Summary: Heme oxygenase-1 has a new cytoprotective role by regulating the complement control protein, which helps prevent kidney injury. Targeting HO-1 induction in specific cells could be a novel approach to attenuate complement-dependent kidney diseases.
Article
Biochemistry & Molecular Biology
Maria G. G. Detsika, Eirini Theochari, Kostas Palamaris, Harikleia Gakiopoulou, Elias A. A. Lianos
Summary: This study investigated the effect of HO-1 absence on the expression of complement regulatory proteins. Using a transgenic rat model lacking HO-1, the results showed reduced expression of DAF and Crry in various tissues except for spleen. C3b deposition was increased in kidney and spleen tissues from HO-1 deficient rats. These findings highlight the important regulatory role of HO-1 in the complement system.
Article
Multidisciplinary Sciences
Xin Gao, Dachuan Zhang, Chunliang Xu, Huihui Li, Kathleen M. Caron, Paul S. Frenette
Summary: Nociceptive nerves play a role in enforcing HSC mobilization through CGRP secretion, collaborating with sympathetic nerves to maintain HSCs in the bone marrow. CGRP directly activates HSCs via the G alpha(s)/adenylyl cyclase/cAMP pathway, and consumption of capsaicin-containing food enhances HSC mobilization in mice.
Article
Physiology
Michelle Schmid, Helena Caria Martins, Gerhard Schratt, Julia M. Kropfl, Christina M. Spengler
Summary: Acute exercise can increase circulating stem and precursor cells in the blood, but the underlying molecular mechanisms are not fully understood. This study found that an increased uptake of miRNA126 in MNCs may lead to reduced levels of RGS16 mRNA, which in turn enhances the translation and secretion of CXCL12. While plasma CXCL12 levels correlated with CPC numbers, the increase in CXCL12 after exercise could not be solely explained by the changes in miRNA126 and RGS16 mRNA levels in MNCs.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Yitong Zhu, Yaqiong Wang, Zhaofeng Lu
Summary: This study explored the efficacy of SDF-1-loaded nanoparticles in traumatic brain injury (TBI) and found that only SDF-1 nanoparticles could induce neural stem/progenitor cells (NSCs) migration to the injured area and enhance healing. These findings suggest the potential application of nanoparticles in the treatment of brain injury.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Kusienicka, Karolina Bukowska-Strakova, Maciej Ciesla, Witold Norbert Nowak, Iwona Bronisz-Budzynska, Agnieszka Seretny, Monika Zukowska, Mateusz Jez, Roscislaw Krutyholowa, Hevidar Taha, Neli Kachamakova-Trojanowska, Halina Was, Claudine Kieda, Alicja Jozkowicz
Summary: It has been found that the expression of MIC markers does not select CSC-like cells in the B16-F10 murine melanoma cell line.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Cassie Bennett, Anabel Alvarez-Ciara, Melissa Franklin, W. Dalton Dietrich, Abhishek Prasad
Summary: The study shows the involvement of the complement cascade in the tissue reaction to neural implants. Modulating the complement cascade may have therapeutic potential in regulating the foreign body response.
Article
Engineering, Biomedical
Lang Chen, Chenyan Yu, Yuan Xiong, Kai Chen, Pei Liu, Adriana C. Panayi, Xiufeng Xiao, Qian Feng, Bobin Mi, Guohui Liu
Summary: Researchers developed an injectable, self-healing, adhesive hydrogel that combines Stromal Cell-Derived Factor-1 alpha (SDF-1 alpha) and M2 macrophage derived exosomes (M2D-Exos). This hydrogel enhanced proliferation and migration of human bone marrow mesenchymal stem cells and human umbilical vein endothelial cells, promoting osteogenesis and angiogenesis both in vivo and in vitro. It provides a new modality for accelerating bone repair by coupling osteogenesis, angiogenesis, and resisting infection at all stages.
BIOACTIVE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Xu Wang, XinXin Han, Yi Qiu, Jianbo Sun
Summary: Stem cell-based therapy shows promise for repairing injured tissue. This study developed a magnetic nanoparticle delivery system for recruiting stem cells using SDF-1(alpha). The results suggest that SDF-MNP has a chemotactic effect on stem cells without cytotoxicity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Farzad Sadri, Zohreh Rezaei, Mohammad Fereidouni
Summary: This review summarizes the molecular signature of the SDF-1/CXCR4 pathway and explains how this axis is involved in normal events.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Wu-Xun Chen, Nai-Chen Cheng, Yan-Jun Chen, I-Chi Lee
Summary: In this study, silk fibroin-based microneedle patches were fabricated as carriers for adipose stem cell recruitment. The crystallinity of silk fibroin-based microneedles was determined after water annealing treatment. The results showed that the microneedle patches had sufficient strength to penetrate the skin and demonstrated sustained release of the model drug. Dual-layer microneedles loaded with SDF-1 were prepared to enhance drug release for stem cell recruitment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Mirca S. Saurty-Seerunghen, Thomas Daubon, Lea Bellenger, Virgile Delaunay, Gloria Castro, Joris Guyon, Ahmed Rezk, Sylvie Fabrega, Ahmed Idbaih, Fabien Almairac, Fanny Burel-Vandenbos, Laurent Turchi, Eric Duplus, Thierry Virolle, Jean-Michel Peyrin, Christophe Antoniewski, Herve Chneiweiss, Elias A. El-Habr, Marie-Pierre Junier
Summary: Cell motility is critical for tumor malignancy. Analysis of transcriptomes from glioblastoma patients identified metabolic weaknesses shared by motile cells, characterized by enhanced mitochondrial load, oxidative stress, and dependence on increased ROS production and MPST activity for their motility.
CELL DEATH & DISEASE
(2022)
Article
Biology
Astrid S. Jorgensen, Viktorija Daugvilaite, Katia De Filippo, Christian Berg, Masa Mavri, Tau Benned-Jensen, Goda Juzenaite, Gertrud Hjorto, Sara Rankin, Jon Vabeno, Mette M. Rosenkilde
Summary: Plerixafor exhibits biased action by reversing the CXCL12 gradient across the bone marrow endothelium and showing superior therapeutic effect in HSC mobilization. In contrast, AMD11070, despite having superior antagonism, does not perform as effectively in HSC mobilization in vivo.
COMMUNICATIONS BIOLOGY
(2021)
Review
Hematology
Nicola Piccirillo, Rossana Putzulu, Elisabetta Metafuni, Giuseppina Massini, Federica Fatone, Andrea Corbingi, Sabrina Giammarco, Maria Assunta Limongiello, Alessia Di Giovanni, Gina Zini, Andrea Bacigalupo, Luciana Teofili, Simona Sica, Patrizia Chiusolo
Summary: A study shows that early estimation of the success of peripheral blood stem cell collection can be achieved through genetic analysis and testing in donors, preventing risks and costs associated with suboptimal mobilization.
TRANSFUSION MEDICINE REVIEWS
(2023)
Article
Cell & Tissue Engineering
Mariusz Z. Ratajczak, Mateusz Adamiak, Janina Ratajczak, Magda Kucia
Summary: Evidence suggests that the trafficking of HSPCs and their progeny is regulated by inflammatory cues, with HO-1 playing a key role in modulating the response to pro-inflammatory stimuli. Inhibition of HO-1 activity may enhance mobilization and homing of normal HSPCs, while its activation could potentially prevent the unwanted spread and in vivo expansion of leukemic cells.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kamila Bujko, Mateusz Adamiak, Ahmed Abdelbaset-Ismail, Arjun Thapa, Nicoletta Ilowska, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: This article investigates the role of P2X1 receptor in the trafficking of hematopoietic stem progenitor cells (HSPCs). Through in vitro and in vivo murine models, it is found that deficiency in P2X1 receptor may lead to difficulties in HSPCs homing and could be associated with the intracellular pattern recognition receptor Nlrp3.
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
(2022)
Meeting Abstract
Hematology
Ahmed Abdelbaset-Ismail, Arjun Thapa, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Meeting Abstract
Hematology
Vira Chumak, Andrzej Ciechanowicz, Mateusz Adamiak, Arjun Thapa, Ahmed Abdelbaset-Ismail, Kamila Bujko, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Meeting Abstract
Hematology
Kamila Bujko, Mateusz Adamiak, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Article
Biochemical Research Methods
Kamila Bujko, Andrzej K. Ciechanowicz, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: Very small embryonic like stem cells (VSELs) are dormant stem cells deposited during embryogenesis in various tissues, including bone marrow. They are released and circulate in peripheral blood at a low level, but increase in number in response to stressors and tissue/organ damage. This study evaluates CD34(+)Lin(-)CD45(-) and CD133(+)Lin(-)CD45(-) VSELs derived from umbilical cord blood, comparing their molecular and proteomic characteristics.
Article
Oncology
Mariusz Z. Ratajczak, Mateusz Adamiak, Ahmed Abdelbaset-Ismail, Kamila Bujko, Arjun Thapa, Vira Chumak, Stephanie Franczak, Katarzyna Brzezniakiewicz-Janus, Janina Ratajczak, Magdalena Kucia
Correction
Oncology
Alison Domingues, Elisa Rossi, Kamila Bujko, Gregoire Detriche, Ulysse Richez, Adeline Blandinieres, Magdalena Kucia, Janina Ratajczak, David M. Smadja, Mariusz Z. Ratajczak
Article
Cell & Tissue Engineering
Arjun Thapa, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: Hematopoietic stem/progenitor cells (HSPCs) express receptors for complement cascade (ComC) cleavage fragments C3a and C5a and respond to inflammation-related cues by sensing PAMPs and DAMPs. The activation of Nox2-ROS-Nlrp3 inflammasome by ComC-derived C3a and C5a plays a role in regulating the responsiveness of HSPCs to stress. Additionally, ComC expressed in HSPCs, known as the complosome, may also contribute to regulating hematopoiesis.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Ahmed Abdelbaset-Ismail, Katarzyna Brzezniakiewicz-Janus, Arjun Thapa, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Summary: The study reveals that hematopoietic stem progenitor cells (HSPCs) follow the diurnal circulation rhythm in peripheral blood and melatonin can inhibit the migration of HSPCs by inhibiting Nlrp3 inflammasome activation.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Ahmed Abdelbaset-Ismail, Andrzej K. Ciechanowicz, Kamila Bujko, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: Proliferation, metabolism, and migration of hematopoietic stem/progenitor cells (HSPCs) are regulated by receptors integrated into membrane lipid rafts (MLRs). The synthesis of MLRs components in HSPCs is regulated by Nox2-ROS-Nlrp3 inflammasome axis, which modulates lipogenesis and the responsiveness of HSPCs to external stimuli.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Himi Tripathi, Alison Domingues, Renee Donahue, Audrey Cras, Coralie L. Guerin, Erhe Gao, Bryana Levitan, Mariusz Z. Ratajczak, David M. Smadja, Ahmed Abdel-Latif, Wadea M. Tarhuni
Summary: Ischemic heart disease, often caused by AMI, is a major cause of morbidity and mortality worldwide. The combination therapy of MSCs and ECFCs can reduce cardiomyocyte apoptosis, scar size, and adverse cardiac remodeling in a pre-clinical model of AMI. This approach shows promise for future human studies during the acute phase of ischemic cardiac injury.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Vira Chumak, Katarzyna Sielatycka, Andrzej Ciechanowicz, Kamila Bujko, Mariusz Z. Ratajczak, Magdalena Kucia
Summary: Very small embryonic-like stem cells (VSELs) are dormant stem cells in adult tissues that contribute to tissue/organ repair and regeneration. Proteomic analysis revealed that VSELs express proteins involved in development, signal transduction, and cellular functions, supporting their origin from reproductive lineage.
STEM CELL REVIEWS AND REPORTS
(2023)
Meeting Abstract
Hematology
Kamila Bujko, Mateusz Adamiak, Ahmed Abdelbaset-Ismail, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Meeting Abstract
Hematology
Andrzej Ciechanowicz, Ahmed Abdelbaset-Ismail, Magda Kucia, Mariusz Z. Ratajczak
Article
Cell & Tissue Engineering
Arjun Thapa, Ahmed Abdelbaset-Ismail, Vira Chumak, Mateusz Adamiak, Katarzyna Brzezniakiewicz-Janus, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: We discovered that sterile inflammation in the bone marrow plays a crucial role in the mobilization, homing, and engraftment of hematopoietic stem/progenitor cells (HSCPs). Extracellular adenosine (eAdo), generated from the conversion of extracellular adenosine triphosphate (eATP), affects HSPCs trafficking by activating the A(2B) receptor. By upregulating heme oxygenase-1 (HO-1), an inhibitor of Nlrp3 inflammasome, the A(2B) receptor inhibition promotes cell migration and proliferation. Blocking the A(2B) receptor could potentially enhance HSPCs mobilization and hematopoietic reconstitution.
STEM CELL REVIEWS AND REPORTS
(2022)