Article
Environmental Sciences
Gerhard P. Aigner, Pamela Nenning, Birgit Fiechtner, Maja Srut, Martina Hoeckner
Summary: This study found that the demethylating agent Aza inhibits gene activity while Cd induces MT2 gene expression, but neither has an effect on global DNA methylation levels. Thus, Cd detoxification based on MT2 induction is not related to changes in DNA methylation.
Article
Chemistry, Multidisciplinary
Eva Korytiakova, Ewelina Kaminska, Markus Mueller, Thomas Carell
Summary: The synthesis of a fluorinated fdC feeding probe allowed for the study of direct deformylation to F-dC, providing insights into the Tdg-independent removal process. The research demonstrated deformylation occurring in both stem cells and different somatic cell types. Additionally, active demethylation was investigated in a non-dividing neurogenin-inducible system of iPS cells differentiating into bipolar neurons.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Agronomy
Shuo Liu, Yu Bao, Hui Deng, Guanqing Liu, Yangshuo Han, Yuechao Wu, Tao Zhang, Chen Chen
Summary: DNA methylation is a crucial epigenetic modification that regulates gene expression and genome stability. The methylation inhibitor, AzaD, can effectively reduce genome-wide hypomethylation and cause a series of epigenetic variations.
Article
Biochemistry & Molecular Biology
Karolina Luczkowska, Piotr Kulig, Klaudia Rusinska, Bartlomiej Baumert, Boguslaw Machalinski
Summary: This study investigates the effects of a methylation inhibitor on the proliferation of multiple myeloma cells and the development of resistance to treatment, finding that alterations in DNA methylation are associated with drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yang-Jie Zhang, Ran Xie, Jie Jiang, Li Zhai, Cong-Hui Yang, Jing Zhang, Xi Wang, Dong-Xue Chen, Hua-Tao Niu, Long Chen
Summary: The study demonstrates the methylation modification of GAS5 in melanoma and the reversible effect of 5-Aza-dC on this modification. Treatment with 5-Aza-dC inhibits cell proliferation, increases apoptosis, and reduces migration and invasion in melanoma cells. The antitumor effect of 5-Aza-dC in melanoma is related to the methylation modification of GAS5.
Article
Chemistry, Multidisciplinary
Eva Korytiakova, Andreas Reichl, Markus Mueller, Thomas Carell
Summary: Cellular DNA consists of four canonical nucleosides and 5-methylcytosine, with stem cells' genome containing three additional dC derivatives formed through oxidation reactions. These derivatives are believed to undergo a cycle of methylation, oxidation, and conversion back to dC within the genome.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Alain Aguirre-Vazquez, Luis A. Salazar-Olivo, Xochitl Flores-Ponce, Ana L. Arriaga-Guerrero, Dariela Garza-Rodriguez, Maria E. Camacho-Moll, Ivan Velasco, Fabiola Castorena-Torres, Nidheesh Dadheech, Mario Bermudez de Leon
Summary: By utilizing epigenetic molecules and small molecules, the expression of pluripotency genes can be induced and methylation in the OCT4 promoter can be reduced in human somatic cells, enhancing reprogramming efficiency. Further studies are needed to elucidate the mechanisms by which these compounds exert their effects on reprogramming.
Article
Multidisciplinary Sciences
Reem Miari, Naiel Azzam, Rinat Bar-Shalom, Fuad Fares
Summary: 5-Aza-2'-deoxycytidine has shown effectiveness in treating HPV(-) HNSCC by inhibiting proliferation and inducing apoptosis. Future studies are needed to clarify the molecular mechanism of action of 5-AzaD in HPV(-) cancer cells.
Article
Biochemistry & Molecular Biology
Chirada Dusadeemeelap, Thira Rojasawasthien, Takuma Matsubara, Shoichiro Kokabu, William N. Addison
Summary: This study investigates the role of TET-mediated DNA hydroxymethylation in bone formation using a novel selective TET enzyme inhibitor, BC339. The results show that inhibiting TET with BC339 suppresses osteoblast differentiation and functions through increasing the methylation level of the Sp7 promoter.
Article
Agriculture, Multidisciplinary
Xiaoqi Wang, Dongli Li, Yong Cao, Chi-Tang Ho, Qingrong Huang
Summary: The research found that 5-OH Sin is mostly accumulated in the large intestine, indicating poor absorption in the small intestine. In addition, the C-3' position of 5-OH Sin is more susceptible to demethylation in systemic circulation. Furthermore, methylation transformation of 5-OH Sin and its metabolites was also observed, suggesting bidirectional biotransformation between 5-OH Sin and its parent compound, Sin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Developmental Biology
Yoshitomo Ueda, Takashi Nakamura, Jing Nie, Alexander J. Solivais, John R. Hoffman, Becca J. Daye, Eri Hashino
Summary: The inner ear sensory epithelia consist of hair cells and supporting cells, which arise from SOX2-expressing prosensory cells. The mechanisms underlying the diversification of these cell lineages remain unclear.
Article
Oncology
Ratnakar Singh, Zeeshan Fazal, Emmanuel Bikorimana, Raya Boyd, Cliff Yerby, Megan Tomlin, Hannah Baldwin, Doha Shokry, Andrea K. Corbet, Khadeeja Shahid, Aleyah Hattab, Sarah J. Freemantle, Michael J. Spinella
Summary: TGCTs exhibit reciprocal sensitivity to hypomethylating agents and cisplatin, with resistant cells showing opposite responses. The downregulation of polycomb target gene expression in hypomethylating agent resistant cells contrasts with the upregulation in cisplatin resistant cells, indicating a complex relationship between epigenetic changes and drug sensitivity in TGCTs.
MOLECULAR ONCOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Joana Alpoim-Moreira, Anna Szostek-Mioduchowska, Magda Slyszewska, Maria Rosa Rebordao, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: Endometrosis is a major cause of infertility in mares, characterized by excessive deposition of collagen and alpha smooth muscle actin in the endometrium. Epigenetic alterations in fibroblast phenotype are linked with the development of endometrial fibrosis. This study investigated the in vitro epigenetic regulation of mare endometrial fibrogenesis through DNMTs transcription and the effect of 5-aza-2'-deoxycytidine on collagen expression in fibroblasts challenged with TGF-beta 1. The findings suggest the potential role of epigenetic mechanisms in equine endometrial fibrogenesis and highlight the therapeutic potential of 5-aza-dC.
Review
Immunology
Alison J. Sinclair
Summary: DNA methylation at CpG motifs plays a key role in gene regulation, with hypermethylation generally leading to decreased gene expression. In EBV infection, the viral genome becomes hypermethylated at CpG motifs, impacting viral gene expression. DNA methyl transferase enzymes are crucial in the process of DNA methylation and can be targeted for therapeutic interventions in EBV-associated cancer.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michael J. Hitchler, Frederick E. Domann
Summary: The development of multicellular organisms involves a complex genetic program controlled by an epigenetic state, with inputs affecting changes in the epigenetic landscape remaining unknown. Dynamic changes in cellular metabolism, redox, free radical production, and oxygen availability are associated with development, impacting gene expression. Oxygen, metabolism, and reactive oxygen species influence redox signaling to limit key epigenetic cofactors, suggesting a close relationship between these factors and epigenetic marks.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Neurosciences
C. B. Hellmer, Y. Zhou, B. Fyk-Kolodziej, Z. Hu, T. Ichinose
Article
Cell & Tissue Engineering
Xiaoyang Li, Alicia Aleardi, Jue Wang, Yang Zhou, Rodrigo Andrade, Zhengqing Hu
STEM CELLS AND DEVELOPMENT
(2016)
Article
Neurosciences
Yang Zhou, Zhengqing Hu
FRONTIERS IN CELLULAR NEUROSCIENCE
(2016)
Article
Multidisciplinary Sciences
Zhengqing Hu, Zhenjie Liu, Xiaoyang Li, Xin Deng
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Zhenjie Liu, Zhengqing Hu
SCIENTIFIC REPORTS
(2018)
Article
Cardiac & Cardiovascular Systems
Won Hee Lee, Sang-Ging Ong, Yang Zhou, Lei Tian, Hye Ryeong Bae, Natalie Baker, Adam Whitlatch, Leila Mohammadi, Hongchao Guo, Kari C. Nadeau, Matthew L. Springer, Suzaynn F. Schick, Aruni Bhatnagar, Joseph C. Wu
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2019)
Article
Multidisciplinary Sciences
Xin Deng, Zhenjie Liu, Xiaoyang Li, Yang Zhou, Zhengqing Hu
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Hye Ryoun Jang, Hyung Joon Cho, Yang Zhou, Ning-Yi Shao, Kyungho Lee, Hoai Huong Thi Le, Junseok Jeon, Jung Eun Lee, Wooseong Huh, Sang-Ging Ong, Won Hee Lee, Yoon-Goo Kim
Summary: The study aimed to develop a novel model of uremic vasculopathy and investigate the effects of uremic toxins on iPSC-derived endothelial cells. The findings demonstrated that simplified uremic toxin mixtures can simulate uremic vasculopathy effectively and CKD patient-specific iPSC-ECs may recapitulate susceptibility to uremic vasculopathy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Xiaoming Ouyang, Yu Liu, Yang Zhou, Jing Guo, Tzu-Tang Wei, Chun Liu, Bomi Lee, Binbin Chen, Angela Zhang, Kerriann M. Casey, Lin Wang, Nigel G. Kooreman, Aida Habtezion, Edgar G. Engleman, Joseph C. Wu
Summary: iPSC-based cancer vaccine shows promising results in preventing tumor formation, activating immune responses, and reducing immunosuppressive cells in preclinical models, supporting further studies in clinical settings.
Article
Cardiac & Cardiovascular Systems
Shiqiao Ye, Cankun Wang, Zhaohui Xu, Hui Lin, Xiaoping Wan, Yang Yu, Subhodip Adhicary, Joe Z. Zhang, Yang Zhou, Chun Liu, Matthew Alonzo, Jianli Bi, Angelina Ramirez-Navarro, Isabelle Deschenes, Qin Ma, Vidu Garg, Joseph C. Wu, Ming-Tao Zhao
Summary: This study demonstrates that NOTCH1 is crucial for the differentiation and proliferation of human cardiac cells. The absence of NOTCH1 leads to defective ventricular-like cardiomyocyte differentiation and impaired cell proliferation. These findings shed light on the mechanisms underlying hypoplastic left heart syndrome.
CIRCULATION RESEARCH
(2023)
Article
Cell & Tissue Engineering
Peter-James H. Zushin, Yang Zhou, Audrey Li, Euan A. Ashley, Matthew T. Wheeler, Joseph C. Wu
Summary: Dilated cardiomyopathies (DCM) are a leading cause of heart failure in aging populations. The chaperone protein BAG3 plays a significant role in the development of DCM and the rate at which it progresses to heart failure. In this study, two human induced pluripotent stem cell (iPSC) lines were generated from individuals with mutations in exon 3 of the BAG3 gene and their pluripotency and ability to differentiate into three primary germ layers were validated. These cell lines serve as a valuable model for studying BAG3 inactivation and insufficiency in relation to DCM.
STEM CELL RESEARCH
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Mingtao Zhao, Shiqiao Ye, Joe Zhang, Ningyi Shao, Chun Liu, Yang Zhou, Masataka Nishiga, Yang Yang, Bianxiao Cui, Vidu Garg, Joseph C. Wu
CIRCULATION RESEARCH
(2020)