Review
Cell Biology
Rui-Dong Cheng, Wen Ren, Ben-Yan Luo, Xiang-Ming Ye
Summary: Spinal cord injury is a complex condition with no effective intervention, where neural repair and regeneration play a vital role in enhancing neurological function. Purinergic receptors, widely expressed in the spinal cord, mediate intracellular and extracellular signaling pathways to improve neural repair and regeneration by responding to ATP release after injury. This article reviews the mechanistic roles of purinergic receptors in spinal cord injury and highlights their potential as intervention targets for neural repair and regeneration.
NEURAL REGENERATION RESEARCH
(2023)
Review
Cell Biology
Batoul Ghaddar, Luisa Luebke, David Couret, Sepand Rastegar, Nicolas Diotel
Summary: Adult neurogenesis is a conserved process in all vertebrates, but differences exist in the number of neurogenic niches, NSC identity, and regenerative capacity between zebrafish and mammals. Zebrafish has a higher number of neurogenic niches and displays strong regenerative capacity without scar formation, unlike mammals. Shared early events after brain injury include cell death, microglia recruitment, oligodendrocyte recruitment, and injury-induced neurogenesis, but mammals form a persistent glial scar whereas zebrafish do not, contributing to their higher regenerative capacity.
Review
Cell Biology
Sara Mercurio, Linda Serra, Miriam Pagin, Silvia K. Nicolis
Summary: This article reviews the diverse phenotypes resulting from conditional Sox2 knockout in different regions of the developing and postnatal brain in mice. It is found that despite the widespread expression of Sox2 in neural stem/progenitor cells, some regions (such as the hippocampus and ventral forebrain) are more vulnerable to Sox2 deletion. The stage of Sox2 deletion also plays a critical role in the resulting defects, indicating a stage-specificity of SOX2 function.
Article
Biochemistry & Molecular Biology
Chantal Ripoll, Gaetan Poulen, Robert Chevreau, Nicolas Lonjon, Florence Vachiery-Lahaye, Luc Bauchet, Jean-Philippe Hugnot
Summary: Ependymal cells in the spinal cord play a crucial role in providing a physical barrier and circulating cerebrospinal fluid. They exhibit a dorsal-ventral expression pattern of spinal cord developmental transcription factors and express neurodevelopmental genes throughout human life. The persistence of ependymal cells and their heterogeneity highlight the importance of further investigation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Immunology
Yanzhi Jiang, Jianxin Lin, Haiyun Zheng, Ping Zhu
Summary: This review examines the role of purinergic signaling in ischemia-reperfusion injury and rejection after heart transplantation, as well as its clinical applications and prospects.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Biomedical
Mihyeon Bae, Do Won Hwang, Min Kyung Ko, Yeona Jin, Woo Jung Shin, Wonbin Park, Suhun Chae, Hong Jun Lee, Jinah Jang, Hee-Gyeong Yi, Dong Soo Lee, Dong-Woo Cho
Summary: Traumatic brain injury is a major cause of accidental death and disability, leading to edema, neuronal apoptosis, and neuroinflammation. Stem cell therapy, particularly using brain-derived decellularized extracellular matrix (BdECM) bioink, shows promise in promoting regeneration in a traumatically injured brain by improving cell survival and differentiation. Experimental results demonstrate that BdECM bioink supports the full differentiation of neural stem cells into neurons and suppresses neuroinflammation, potentially aiding in the successful regeneration of a traumatically injured brain.
Review
Biochemistry & Molecular Biology
Yibin Jia, Guanyi Wang, Yuqing Ye, Enming Kang, Huijun Chen, Zishuo Guo, Xiaosheng He
Summary: TBI is a disease with high morbidity and disability worldwide, despite ongoing neural protection efforts. Due to patient heterogeneity, it is difficult to achieve satisfying results in clinic. Neuroinflammation appears since primary injury occurs, and recent studies suggest NSCs play a central role in neural regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lina Mariana Tovar, Carlos Felipe Burgos, Gonzalo E. Yevenes, Gustavo Moraga-Cid, Jorge Fuentealba, Claudio Coddou, Luisa Bascunan-Godoy, Claudio Catrupay, Angel Torres, Patricio A. A. Castro
Summary: Neurulation is a crucial process in the formation of the central nervous system (CNS). Connexins (Cxs), which form gap junctions (GJs) and hemichannels (HCs), play a role in neural tube development. ATP release mediated by HCs-Cxs is important during the neurulation process. Blockade of HCs-Cxs can lead to neural tube defects (NTDs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Jun Zhou, Yaqi Wu, Zhijian Tang, Kaipeng Zou, Juan Chen, Zuowei Lei, Xueyan Wan, Yanchao Liu, Huaqiu Zhang, Yu Wang, Armin Blesch, Ting Lei, Shengwen Liu
Summary: Studies have shown that alginate capillary hydrogels can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can survive, proliferate, and differentiate into neurons within the capillaries in vitro. In animal experiments, a portion of the grafted cells can survive and differentiate into neurons, astrocytes, and oligodendrocytes. Furthermore, the grafted cells can promote the growth of host axons, form putative synapses with host neurons, improve electrophysiological conductivity, and partially restore locomotor function.
REGENERATIVE BIOMATERIALS
(2022)
Article
Neurosciences
Xuecheng Qiu, Yaling Guo, Ming-Feng Liu, Bingge Zhang, Jingzhen Li, Jian-Feng Wei, Meng Li
Summary: Traumatic brain injury (TBI) is a major cause of long-term disability in young adults. This study found that microglia and astrocytes were the most affected cells during the subacute phase of TBI, and non-canonical neurotrophic factors MDK, PTN, and PSAP were upregulated and played a crucial role in neuroregeneration.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Neurosciences
Hae Young Shin, Min Jung Kwon, Eun Mi Lee, Kyung Kim, Young Joo Oh, Hyung Soon Kim, Dong Hoon Hwang, Byung Gon Kim
Summary: The Myc proto-oncogene acts as a transcriptional hub gene in regulating the expression of a distinct subset of regeneration-associated genes (RAGs) in DRGs following preconditioning nerve injury. Deletion of Myc leads to a decrease in injury-induced expression of a specific subset of RAGs, which overlaps with the list of RAGs upregulated by Myc overexpression. Myc overexpression in DRGs post-spinal cord injury prevents sensory axon retraction through downstream RAG June, highlighting the critical role of Myc in axon regeneration.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Bokyung Song, Sung-Joong Lee, Chong-Hyun Kim
Summary: In a ATP-induced cortical injury model, ATP and cytokine TNF-alpha significantly affect the physiological activities of neurons and microglia at day 3 post-injection, while IL-10 and IL-4 effectively suppress these changes in both cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xiao-Yin Liu, Yin-He Feng, Qing-Bo Feng, Jian-Yong Zhang, Lin Zhong, Peng Liu, Shan Wang, Yan-Ruo Huang, Xu-Yi Chen, Liang-Xue Zhou
Summary: This study discovered a novel 3D-printed collagen/chitosan scaffold loaded with exosomes derived from neural stem cells pretreated with insulin-like growth factor-1 (INExos), which significantly improved repair and functional recovery after traumatic brain injury in rats. The findings suggest a potential strategy for the treatment of traumatic brain injury and lay a solid foundation for clinical translation.
NEURAL REGENERATION RESEARCH
(2023)
Article
Medicine, General & Internal
Zhenghui He, Lijian Lang, Jiyuan Hui, Yuxiao Ma, Chun Yang, Weiji Weng, Jialin Huang, Xiongfei Zhao, Xiaoqi Zhang, Qian Liang, Jiyao Jiang, Junfeng Feng
Summary: This study found that around 7 days after TBI in mice, the subacute phase could encourage more neuronal differentiation after transplantation. The autophagy flux played a critical role in regulating neuronal differentiation and could be a potential target to improve the efficacy of transplantation in the early phase.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Neurosciences
Wen -Jun Zhang, Dong-Xia Hu, Si-Jian Lin, Xiao-Qun Fang, Zhen-Feng Ye
Summary: This article discusses the role of P2X receptors in cerebral ischemic injury and the importance of regulating microglial activity. Activation of P2X receptors has been found to induce inflammatory responses and exacerbate neurological dysfunction, while inhibiting the activation of P2X receptors can help protect nerve function.
BRAIN RESEARCH BULLETIN
(2022)
Review
Biochemistry & Molecular Biology
Patricia de Carvalho Ribeiro, Lucas Felipe Oliveira, Daniel Mendes Filho, Ricardo Cambraia Parreira, Mariana Sousa Vieira, Bruno Lemes Marques, Elis Marra da Madeira Freitas, Walison N. N. Silva, Helton da Costa Santiago, Alexander Birbrair, Henning Ulrich, Valdo Jose Dias da Silva, Rodrigo R. R. Resende
Summary: Systemic arterial hypertension is a major risk factor for cardiovascular and renal diseases, and early recognition is important for prevention. The study of biomarkers is an important approach for diagnosing and predicting hypertension and related conditions. This review discusses the use of biomarkers in hypertension and associated diseases such as ischemic stroke, intracerebral hemorrhage, transient ischemic attack, acute myocardial infarction, angina pectoris, and chronic kidney disease. Establishing a pool of potential biomarkers can improve diagnosis, prognosis, risk assessment, therapy management, and pharmacological responses to enhance patients' quality of life and prevent unfavorable outcomes.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Physiology
Marcela Guimaraes Takahashi De Lazari, Celso Tarso Rodrigues Viana, Luciana Xavier Pereira, Laura Alejandra Ariza Orellano, Henning Ulrich, Silvia Passos Andrade, Paula Peixoto Campos
Summary: The study found that sodium butyrate can attenuate the inflammatory, angiogenesis, and fibrogenesis processes involved in peritoneal fibrosis, indicating its potential for improving the condition. This finding contributes to the development of new medications for treating peritoneal fibrosis.
EXPERIMENTAL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Karina Scheuermann, Celso Tarso Rodrigues Viana, Diego Carlos dos Reis, Marcela Guimaraes Takahashi de Lazari, Laura Alejandra Ariza Orellano, Clara Tolentino Machado, Leticia Cristine Cardoso dos Santos, Henning Ulrich, Luciano Santos Aggum Capettini, Silvia Passos Andrade, Paula Peixoto Campos
Summary: The effects of amitriptyline (AM) on foreign body reaction (FBR) after biomedical material implantation are not known. This study found that AM can inhibit early angiogenesis and fibrogenesis of the implants, but has no significant effects on 7-day-old implants.
Article
Neurosciences
Deidiane Elisa Ribeiro, Lyvia Lintzmaier Petiz, Talita Glaser, Agatha Oliveira-Giacomelli, Roberta Andrejew, Fernando de Azevedo Ribeiro Saab, Milena da Silva Milanis, Henrique Correia Campos, Vanessa Fernandes Arnaud Sampaio, Sophia La Banca, Beatriz Monteiro Longo, Claudiana Lameu, Yong Tang, Rodrigo Ribeiro Resende, Sergio T. Ferreira, Henning Ulrich
Summary: About 10 million new cases of dementia occur globally each year, with up to 70% attributed to Alzheimer's disease (AD). AD patients often experience non-cognitive symptoms known as behavioral and psychological symptoms of dementia (BPSDs), including sleep disorders and mood alterations such as depression and apathy. BPSDs have a significant impact on AD patients, caregivers, public health systems, and the economy. Understanding the role of purinergic signaling, particularly the A1, A2A, and P2X7 receptors, in AD may provide new therapeutic approaches for the disease and related conditions.
Review
Biochemistry & Molecular Biology
Stephanie Franczak, Henning Ulrich, Mariusz Z. Z. Ratajczak
Summary: Hematopoiesis is regulated by various mediators, including peptide-based growth factors, cytokines, and chemokines, as well as purinergic signaling pathways and the complement cascade. Recent studies have shown that these pathways interact with specific receptors expressed on hematopoietic stem/progenitor cells (HSPC). In addition, evidence suggests that some of these receptors can be activated by intrinsically expressed ligands. Furthermore, activation of the Nox2-ROS-NLRP3 axis coordinates the responses mediated by purinergic signaling and the ComC network.
PURINERGIC SIGNALLING
(2023)
Editorial Material
Neurosciences
Qing Ye, Si-Si Lin, Henning Ulrich, Yong Tang
NEUROSCIENCE BULLETIN
(2023)
Article
Polymer Science
Emilli C. G. Frachini, Jessica S. G. Selva, Paula C. Falcoswki, Jean B. Silva, Daniel R. Cornejo, Mauro Bertotti, Henning Ulrich, Denise F. S. Petri
Summary: CAF was loaded into Gel/Alg/MNP hydrogels to achieve sustained release, and the presence of Ca2+ and EMF enhanced the release of CAF. The released concentration of CAF from Gel/Alg/MNP hydrogels in PBS/CaCl2 at 37°C was 0.35 mM, promoting cell viability.
Article
Neurosciences
Henrique Correia Campos, Deidiane Elisa Ribeiro, Debora Hashiguchi, Talita Glaser, Milena da Silva Milanis, Christiane Gimenes, Deborah Suchecki, Ricardo Mario Arida, Henning Ulrich, Beatriz Monteiro Longo
Summary: The aim of this study was to investigate the effects of resistance exercise on preventing and recovering from AD-related neuropathological conditions in APP/PS1 mice. The results showed that 4 weeks of resistance exercise improved locomotor activity and memory in APP/PS1 mice, and reduced A beta plaques and systemic inflammation. These findings suggest that resistance exercise plays a role in alleviating AD symptoms and can be an effective complementary treatment for AD.
FRONTIERS IN NEUROSCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Marta Boccazzi, Stefano Raffaele, Thomas Zanettin, Maria P. Abbracchio, Marta Fumagalli
Summary: Neurodevelopmental disorders (NDDs) encompass a range of congenital pathological conditions that affect cognitive, social behavior, and sensory/motor functioning. Recent research has linked genetic disorders involving purine metabolism to autism-like behaviors in humans. Dysregulated purine and pyrimidine levels have also been found in biofluids of individuals with other NDDs. Blocking specific purinergic pathways has been shown to reverse cognitive and behavioral defects in rodent models of NDDs. These findings suggest that targeting P2 receptor signaling could lead to the development of more specific treatments and early detection markers for NDDs.
Review
Biochemistry & Molecular Biology
Rebeca Perez, Viviana Burgos, Victor Marin, Antoni Camins, Jordi Olloquequi, Ivan Gonzalez-Chavarria, Henning Ulrich, Ursula Wyneke, Alejandro Luarte, Leandro Ortiz, Cristian Paz
Summary: Neurodegenerative disorders involve the progressive degeneration and death of neurons, and oxidative stress and neuroinflammation play crucial roles in their progression. Two major pathways, Nrf2 and NF-kappa B, have been identified as potential therapeutic targets due to their involvement in oxidative protection and the promotion of cytokine expression, respectively. Caffeic acid phenethyl ester (CAPE), a compound found in propolis, has shown neuroprotective activity by modulating these pathways. This review summarizes the biosynthesis and chemical synthesis of CAPE derivatives and discusses their neuroprotective activity in both in vitro and in vivo studies conducted from 2010 to 2023.
Article
Cell & Tissue Engineering
Micheli Mainardi Pillat, Ana Carolina Ayupe, Guilherme Juvenal, Carine Meinerz, Talita Glaser, Diogo Vieira da Silva Pellegrina, Daisylea Souza Paiva, Carlos Fernando Mello, Beatriz Monteiro Longo, Eduardo Moraes Reis, Henning Ulrich
Summary: Proteins involved in AD, such as APP and PS1, have a critical role in CNS development and immune response. Mutations in these proteins lead to abnormal expression in NPCs and resemble the brain pathology in adult AD patients.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Chemistry, Physical
Alex Carvalho Alavarse, Jean Bezerra Silva, Henning Ulrich, Denise Freitas Siqueira Petri
Summary: Hydrogels of poly(vinyl alcohol) (PVA)/sodium alginate (SA) with randomly distributed magnetic nanoparticles (MNPs) and magnetically oriented MNPs were prepared. Atomic force microscopy (AFM) and magnetic force microscopy (MFM) were used to evaluate the topography and magnetic domains distribution. The results showed that the orientation of magnetic domains led to an increase of surface roughness, which decreased the viability of HT-22 cells. These findings are important for situations where control of cell proliferation is necessary.
Article
Chemistry, Medicinal
Carla Fernanda Furtado Gardani, Eduardo Luiz Pedrazza, Victoria Santos Paz, Gabriele Goulart Zanirati, Jaderson Costa da Costa, Roberta Andrejew, Henning Ulrich, Juliete Nathali Scholl, Fabricio Figueiro, Liliana Rockenbach, Fernanda Bueno Morrone
Summary: This study investigated the expression of CD39 and CD73 enzymes in prostate cancer and their potential as therapeutic targets. The results showed higher levels of CD39 expression compared to CD73 in tissue samples and a correlation with Gleason score. In blood samples, CD39 expression in extracellular vesicles was significantly increased and positively correlated with ADP hydrolysis and Gleason score.
Article
Cell & Tissue Engineering
Yahaira Naaldijk, Lauren S. Sherman, Natalia Turrini, Yannick Kenfack, Mariusz Z. Ratajczak, Nizar Souayah, Pranela Rameshwar, Henning Ulrich
Summary: Neurodegenerative diseases continue to be a significant healthcare problem, and their association with neuroinflammation is well recognized. This study investigated the role of mesenchymal stem cells (MSC) in modulating activated microglial cells through exosome release. The findings suggest that MSC-induced exosomes can suppress the inflammatory response of M1 macrophages while promoting a more balanced inflammation in M2 macrophages. This insight into the potential of MSCs for tissue regeneration and neuroinflammation is valuable.
STEM CELL REVIEWS AND REPORTS
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Onesia Cristina Oliveira-Lima, Gustavo Almeida de Carvalho, Leandro Prado Assuncao, Alexandre Melo Bailao, Henning Ulrich, Antonio Carlos Pinheiro de Oliveira, Alexander Birbrair, Renato Santiago Gomez, Mauro Cunha Xavier Pinto
JOURNAL OF NEUROCHEMISTRY
(2023)