Article
Developmental Biology
Lydvina Meister, Hector Escriva, Stephanie Bertrand
Summary: The FGF signalling pathway plays crucial roles in controlling the fate of anterior paraxial mesoderm cells and the formation of the anterior notochord in vertebrates and cephalochordates. Inhibition of FGF signal pathway results in loss of structures and affects embryonic development.
Article
Biochemistry & Molecular Biology
Shuji Matsuguchi, Yohei Hirai
Summary: Pluripotent stem cells have the capacity to differentiate into all three germ layers. However, when stemness factors are removed, pluripotent stem cells, such as embryonic stem cells (ESCs), exhibit EMT-like behavior and lose stemness signatures. This process involves the membrane translocation of syntaxin4 (Stx4) and the expression of P-cadherin. Extracellular Stx4 induces upregulation of the gastrulation-related gene brachyury and slightly upregulates the smooth muscle cell-related gene ACTA2 in ESCs. Furthermore, extracellular Stx4 prevents the elimination of C/EBP13. These findings highlight the role of extracellular Stx4 in early mesoderm induction and its impact on differentiation state.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xiang Zhao, Bethany N. Radford, Mark Ungrin, Wendy Dean, Myriam Hemberger
Summary: Normal developmental progression relies on close interactions between the embryonic and extraembryonic lineages in the pre- and peri-gastrulation stage conceptus. Trophoblast cells of the extraembryonic ectoderm play a role in delaying cardiomyocyte differentiation dynamics and prolonging pluripotency in embryonic stem cells (ESCs). These findings provide insights into the intricate crosstalk between different compartments of the early conceptus and have implications for synthetic embryo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Xulun Wu, Wentao Zhao, Hao Wu, Qiancheng Zhang, Yiming Wang, Kunyuan Yu, Jinglei Zhai, Fan Mo, Meijiao Wang, Shiwen Li, Xili Zhu, Xiaoyan Liang, Baoyang Hu, Guang-Hui Liu, Jun Wu, Hongmei Wang, Fan Guo, Leqian Yu
Summary: The interactions between extra-embryonic tissues and embryonic tissues are crucial for proper early embryo development. However, the understanding of this crosstalk is limited due to ethical restrictions, difficulty in obtaining natural human embryos, and lack of appropriate in vitro models. Our study showed that by aggregating human embryonic stem cells with human trophoblast stem cells, hESCs self-organized into an asymmetric structure with primitive streak-like cells exclusively located at one end and morphologically flattened cells presumed to be extra-embryonic mesoderm cells induced at the opposite end. This research revealed potential roles of the extra-embryonic trophectoderm in regulating proper primitive streak formation and inducing extra-embryonic mesoderm cells from the human epiblast.
CELL PROLIFERATION
(2023)
Article
Cell & Tissue Engineering
Kasey Y. C. Lau, Hernan Rubinstein, Carlos W. Gantner, Ron Hadas, Gianluca Amadei, Yonatan Stelzer, Magdalena Zernicka-Goetz
Summary: Scientists have developed an in vitro model using mouse embryonic stem cells (ESCs) that can recapitulate mouse embryogenesis, including the interactions between embryonic and extraembryonic tissues. By comparing the transcriptional programs with natural embryos, the model shows remarkable similarity but also some divergences.
Article
Multidisciplinary Sciences
Jaeeun Lim, Eiko Sakai, Fuminori Sakurai, Hiroyuki Mizuguchi
Summary: Research reveals that miR-27b plays an antagonistic role in the early differentiation of human-induced pluripotent stem cells (hiPS cells), impacting cell differentiation and gene expression by inhibiting the bone morphogenetic protein (BMP) signaling pathway.
SCIENTIFIC REPORTS
(2021)
Article
Biology
James Z. J. Kwan, Thomas F. Nguyen, Anuli C. Uzozie, Marek A. Budzynski, Jieying Cui, Joseph M. C. Lee, Filip Van Petegem, Philipp F. Lange, Sheila S. Teves
Summary: Depletion of TBP in mouse embryonic stem cells does not affect ongoing Pol II transcription, but severely impairs Pol III initiation. However, Pol II transcriptional induction still occurs normally upon TBP depletion. This TBP-independent transcription mechanism is not due to functional redundancy with TRF2, but rather the robustness of the TFIID complex and the Pol II machinery.
Article
Multidisciplinary Sciences
Peng-Fei Xu, Ricardo Moraes Borges, Jonathan Fillatre, Maraysa de Oliveira-Melo, Tao Cheng, Bernard Thisse, Christine Thisse
Summary: By instructing mouse embryonic stem cell aggregates with an experimentally engineered morphogen signalling centre, embryo-like structures called embryoids are formed, exhibiting characteristics of a neurula-stage mouse embryo with patterning of germ layer derivatives along both the antero-posterior and dorso-ventral axes.
NATURE COMMUNICATIONS
(2021)
Article
Mathematics, Applied
Jesus Pantoja-Hernandez, Moises Santillan
Summary: Somitogenesis is the process of segmenting presomitic mesoderm into somites along the anterior-posterior axis, driven by a genetic oscillator known as the segmentation clock. Proper synchronization of the segmentation clock among PSM cells is essential for normal somitogenesis, and factors like network size, cell interactions, and variability among cells can affect synchronization. Very small networks may not synchronize, while moderate variability and network connectivity can improve synchronization in large networks.
Article
Immunology
Danfeng Lu, Zhuoyue Li, Pei Zhu, Zhenxing Yang, Heng Yang, Zhanhong Li, Huachun Li, Zhuoran Li
Summary: This study provides insights into the comprehensive characterization of BTV-host interactome and BTV infection and pathogenic mechanisms through whole-transcriptome sequencing and bioinformatics analysis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Hayat Hage, Shingo Miyauchi, Mate Viragh, Elodie Drula, Byoungnam Min, Delphine Chaduli, David Navarro, Anne Favel, Manon Norest, Laurence Lesage-Meessen, Balazs Balint, Zsolt Merenyi, Laura de Eugenio, Emmanuelle Morin, Angel T. Martinez, Petr Baldrian, Martina Stursova, Maria Jesus Martinez, Cenek Novotny, Jon K. Magnuson, Joey W. Spatafora, Sundy Maurice, Jasmyn Pangilinan, Willian Andreopoulos, Kurt LaButti, Hope Hundley, Hyunsoo Na, Alan Kuo, Kerrie Barry, Anna Lipzen, Bernard Henrissat, Robert Riley, Steven Ahrendt, Laszlo G. Nagy, Igor Grigoriev, Francis Martin, Marie-Noelle Rosso
Summary: The research highlights the genomic and functional adaptations to wood decay in Polyporales fungi. Through analysis of genome composition and transcriptome responses, it reveals poor conservation in macrosynteny among different phylogenetic clades, gene family expansion/contraction in core polyporoids and phlebioids, and expansions in genes involved in signaling and regulation in the lineages of white rotters. Additionally, novel enzymes such as cupredoxins, thaumatin-like proteins, and lytic polysaccharide monooxygenases were identified as potential new candidates for wood decomposition.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Cell Biology
Noriyuki Satoh, Hitoshi Tominaga, Masato Kiyomoto, Kanako Hisata, Jun Inoue, Koki Nishitsuji
Summary: Amphioxus, specifically Branchiostoma japonicum, shows distinct expression patterns and levels of the two Brachyury genes in different embryonic regions, indicating their unique roles in development. Bra2 appears to be the ancestral Brachyury gene in amphioxus, while Bra1 likely evolved to play a supplementary function in notochord and somite formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Weisheng Zheng, Liping Wang, Wenteng He, Xinjie Hu, Qianshu Zhu, Liang Gu, Cizhong Jiang
Summary: Haploid embryonic stem cells (haESCs) have similar transcriptome profiles to naive pluripotent stem cells and exhibit a similar chromatin structure to embryonic stem cells (ESCs). However, haESCs lack a nucleosome depletion region found in ESCs and have different chromatin states in enhancers related to the regulation of the cell cycle. These findings suggest that haESCs have unique characteristics and mechanisms compared to differentiated cell types.
CELL PROLIFERATION
(2023)
Article
Biotechnology & Applied Microbiology
Anupama Jha, Mathieu Quesnel-Vallieres, David Wang, Andrei Thomas-Tikhonenko, Kristen W. Lynch, Yoseph Barash
Summary: This study identifies pervasive features of RNA-processing gene deregulation and aberrant splicing across a wide array of solid tumor types, which might converge on core cancer pathways.
Article
Biochemistry & Molecular Biology
Yu You, Yandi Cui, Yuting Li, Meng Zhang, Xin Wang, Junxiang Ji, Xinbao Zhang, Min Zhou, Zhonglin Zhang, Shou-Dong Ye, Xiaoxiao Wang
Summary: The study investigated the characteristics of MTA family members in human ESCs and found that knockdown of MTA2 and MTA3 expression induces differentiation. Decreased MTA2 or MTA3 gene transcript mainly leads to the emergence of mesendoderm associated markers. Addition of ID8, a specific inhibitor of DYRKs, impairs the differentiation phenotype induced by MTA2 and MTA3 reduction.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Tobias Hannes, Marie Wolff, Michael Xavier Doss, Kurt Pfannkuche, Moritz Haustein, Jochen Mueller-Ehmsen, Agapios Sachinidis, Juergen Hescheler, Markus Khalil, Marcel Halbach
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2015)
Article
Multidisciplinary Sciences
R. Gabdoulline, W. Kaisers, A. Gaspar, K. Meganathan, M. X. Doss, S. Jagtap, J. Hescheler, A. Sachinidis, H. Schwender
Review
Cardiac & Cardiovascular Systems
John Antonydas Gaspar, Michael Xavier Doss, Jan Georg Hengstler, Cristina Cadenas, Juergen Hescheler, Agapios Sachinidis
CIRCULATION RESEARCH
(2014)
Article
Cardiac & Cardiovascular Systems
Alexander Burashnikov, Jose M. Di Diego, Serge Sicouri, Michael Xavier Doss, Agapios Sachinidis, Hector Barajas-Martinez, Dan Hu, Yoshino Minoura, N. Sydney Moise, Bruce G. Kornreich, Liguo Chi, Luiz Belardinelli, Charles Antzelevitch
EUROPEAN JOURNAL OF HEART FAILURE
(2014)
Article
Cell Biology
Michael Xavier Doss, Vilas Wagh, Herbert Schulz, Meelis Kull, Raivo Kolde, Kurt Pfannkuche, Tobias Nolden, Heinz Himmelbauer, Jaak Vilo, Juergen Hescheler, Agapios Sachinidis
Article
Cardiac & Cardiovascular Systems
Jonathan M. Cordeiro, Vladislav V. Nesterenko, Serge Sicouri, Robert J. Goodrow, Jacqueline A. Treat, Mayurika Desai, Yuesheng Wu, Michael Xavier Doss, Charles Antzelevitch, Jose M. Di Diego
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2013)
Article
Multidisciplinary Sciences
Kemar Brown, Stephanie Legros, Jerome Artus, Michael Xavier Doss, Raya Khanin, Anna-Katerina Hadjantonakis, Ann Foley
Article
Multidisciplinary Sciences
Michael Xavier Doss, Jose M. Di Diego, Robert J. Goodrow, Yuesheng Wu, Jonathan M. Cordeiro, Vladislav V. Nesterenko, Hector Barajas-Martinez, Dan Hu, Janire Urrutia, Mayurika Desai, Jacqueline A. Treat, Agapios Sachinidis, Charles Antzelevitch
Article
Cell & Tissue Engineering
Celia Conesa, Michael Xavier Doss, Charles Antzelevitch, Agapios Sachinidis, Javier Sancho, Jose Alberto Carrodeguas
STEM CELL REVIEWS AND REPORTS
(2012)
Article
Cell & Tissue Engineering
John Antonydas Gaspar, Michael Xavier Doss, Johannes Winkler, Vilas Wagh, Juergen Hescheler, Raivo Kolde, Jaak Vilo, Herbert Schulz, Agapios Sachinidisl
STEM CELLS AND DEVELOPMENT
(2012)
Article
Cell Biology
V. Wagh, M. X. Doss, D. Sabour, R. Niemann, K. Meganathan, S. Jagtap, J. A. Gaspar, M. A. Ardestani, S. Papadopoulos, M. Gajewski, J. Winkler, J. Hescheler, A. Sachinidis
CELL DEATH & DISEASE
(2014)
Article
Multidisciplinary Sciences
Irene Aksoy, Vincent Giudice, Edwige Delahaye, Florence Wianny, Maxime Aubry, Magali Mure, Jiaxuan Chen, Ralf Jauch, Gireesh K. Bogu, Tobias Nolden, Heinz Himmelbauer, Michael Xavier Doss, Agapios Sachinidis, Herbert Schulz, Oliver Hummel, Paola Martinelli, Norbert Huebner, Lawrence W. Stanton, Francisco X. Real, Pierre-Yves Bourillot, Pierre Savatier
NATURE COMMUNICATIONS
(2014)
Review
Cell Biology
Michael Xavier Doss, Agapios Sachinidis
Review
Cell Biology
Michael Simeon, Seema Dangwal, Agapios Sachinidis, Michael Xavier Doss
Summary: Personalized regenerative medicine and biomedical research have been significantly advanced by human pluripotent stem cells along with recent progress in genomics, artificial intelligence, and genome engineering. However, challenges remain in translating pluripotent stem cell research into clinical applications, despite the groundbreaking advancements in mRNA vaccines and space exploration projects.