Review
Biochemistry & Molecular Biology
Sandra Ferreira, Nuno Saraiva, Patricia Rijo, Ana S. Fernandes
Summary: LOX and LOXL2 are amine oxidases that catalyze cross-linking reactions of elastin and collagen in connective tissue, with LOXL2 showing high expression in certain tumors. Inhibition of LOX and LOXL2 is considered a promising strategy for preventing metastasis and invasion of breast cancer. Novel synthetic compounds acting as selective inhibitors for LOX/LOXL2 or dual LOX/LOXL inhibitors have been developed, showing potential for effective therapeutic approaches in breast cancer treatment.
Article
Oncology
Bo Zhang, Chenglin Luo, Wenjin Xiao
Summary: This study showed that the LOX mutant LOXG473A may promote osteoclast formation by enhancing autophagy through the AMPK/mTOR pathway. The autophagy inhibitor 3-MA reduced osteoclast formation and resorption, while the autophagy agonist RAPA reversed these effects, suggesting that promoting autophagy can enhance the ability of LOXG473A to induce osteoclast formation.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ryuhei Takemoto, Tetsuro Kamiya, Taku Atobe, Hirokazu Hara, Tetsuo Adachi
Summary: In M2-like macrophages, both HIF1α and STAT3 are essential for LOX induction, while the loss of FOXO1 facilitates HIF1α binding to the lox promoter region, promoting LOX induction.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Michael Chute, Preetinder K. Aujla, Yingxi Li, Sayantan Jana, Pavel Zhabyeyev, Jaslyn Rasmuson, Caroline A. Owen, Thomas Abraham, Gavin Y. Oudit, Zamaneh Kassiri
Summary: ADAM15 plays an important role in heart disease, and its deficiency can lead to impaired scar formation after myocardial infarction, potentially related to collagen cross-linking and kinase expression.
Article
Physiology
Ying Zhong, Rose C. Mahoney, Zehedina Khatun, Howard H. Chen, Christopher T. Nguyen, Peter Caravan, Jesse D. Jr Jr Roberts
Summary: The study reveals that TGFI3 regulates LOX protein expression in newborn lung tissue via Smad2/3-dependent signaling, and implicates LOXs in the generation of excess aldehydes during neonatal lung injury.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuta Yamamura, Norihiko Sakai, Yasunori Iwata, David Lagares, Akinori Hara, Shinji Kitajima, Tadashi Toyama, Taro Miyagawa, Hisayuki Ogura, Koichi Sato, Megumi Oshima, Shiori Nakagawa, Akira Tamai, Keisuke Horikoshi, Takahiro Matsuno, Naoki Yamamoto, Daiki Hayashi, Yoshitada Toyota, Daichi Kaikoi, Miho Shimizu, Andrew M. Tager, Takashi Wada
Summary: This study reveals the importance of the MRTF-SRF pathway in renal fibrosis by regulating the expression of extracellular matrix components and promoting myofibroblast differentiation. The findings suggest that targeting the MRTF-SRF pathway could be a potential therapeutic strategy for renal fibrosis.
Article
Biochemistry & Molecular Biology
Thomas J. Whitlow, Yu Zhang, Nathan Ferguson, Alexandra M. Perez, Hemchandra Patel, Josephine A. Link-Kemp, Ethan M. Larson, Allison T. Mezzell, Vinit C. Shanbhag, Michael J. Petris, Katherine E. Vest
Summary: Cu is important for biochemical pathways as a cofactor or regulator of enzymes. Its import and distribution are controlled by transporters and metallochaperones, and balance of Cu uptake and export is necessary for Cu homeostasis. Impaired Cu transporters can cause genetic diseases, but little is known about the regulatory mechanisms of Cu fluctuation. Cu plays a crucial role in skeletal muscle differentiation, and increased levels of ATP7A during differentiation promote Cu delivery to lysyl oxidase for myotube formation. These findings reveal a previously unknown role of Cu in muscle differentiation and have implications for understanding Cu-dependent differentiation in other tissues.
Article
Immunology
Veronica Marquez, Grisel Ballesteros, Thomas Dobner, Ramon A. Gonzalez
Summary: Human adenovirus 36 (HAdV-D36) infection can induce obesity in animal models and promote adipocyte differentiation. In this study, the researchers explored the replication cycle of HAdV-D36 in preadipocytes and found that only adipocyte-committed cells supported productive viral replication, leading to a greater impact on adipogenesis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Abhilasha Srivastava, Harshita Sharma, Shibasish Chowdhury, Rajdeep Chowdhury, Sudeshna Mukherjee
Summary: Hepatocellular carcinoma (HCC) often develops under inflammatory conditions with various cytokines present. Transforming growth factor-beta (TGF-beta) is a major cytokine in the tumor environment and is involved in promoting epithelial to mesenchymal transition (EMT) in tumor cells. However, the cellular events and molecular regulation of TGF-beta-induced EMT are still unclear. In this study, HCC cells were treated with TGF-beta and it was found that EMT induced by TGF-beta is associated with cytostasis and altered cellular metabolism. TGF-beta down-regulates cell cycle-associated transcripts and metabolic genes through epigenetic silencing, and this process is mediated by TGF-beta downstream signaling mediator-SMAD and chromatin repressive complex member-EZH2.
Article
Virology
Peng-Yuan Lu, Guo-Juan Niu, Pan-Pan Hong, Jin-Xing Wang
Summary: In shrimp, the LOXL protein plays a crucial role in antiviral and antibacterial innate immunity by enhancing the expression of antimicrobial peptides.
Article
Biochemistry & Molecular Biology
Tamara Rosell-Garcia, Sergio Rivas-Munoz, Alain Colige, Fernando Rodriguez-Pascual
Summary: Members of the lysyl oxidase (LOX) family catalyze the oxidative deamination of lysine and hydroxylysine residues in collagen and elastin. Proteolysis by BMP1 and ADAMTS families regulates LOX enzymes, including LOXL1 associated with pelvic organ prolapse and pseudoexfoliation (PEX) glaucoma. This study reveals the molecular information of LOXL1 proteolysis by BMP1 and ADAMTS14, identifying the processing sites and suggesting complex regulation of LOXL1 function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Dan Li, Xiaofei Wang, Liping Yao, Huaixiang Jing, Tiantian Qin, Mingyue Li, Shuyu Zhang, Zhi Chen, Li Zhang
Summary: Mouse incisors have asymmetric distribution of enamel, allowing for sharp cutting edge on labial side and abrasion on lingual side. Sox2 plays important roles in maintaining stem cell pluripotency and organ formation.
JOURNAL OF MOLECULAR HISTOLOGY
(2021)
Article
Toxicology
Mahdi Bahmani, Nasrin Ziamajidi, Mohammad Hashemnia, Roghayeh Abbasalipourkabir
Summary: This study evaluated the effects of human umbilical cord blood mesenchymal stem cells conditioned medium on Lysyl oxidase family gene expression and proteins, and found that it significantly decreased the expression of LOX genes and proteins, improving liver fibrosis by reducing LOX family gene expression.
Article
Immunology
Difeng Fang, Kairong Cui, Yaqiang Cao, Mingzhu Zheng, Takeshi Kawabe, Gangqing Hu, Jaspal S. Khillan, Dan Li, Chao Zhong, Dragana Jankovic, Alan Sher, Keji Zhao, Jinfang Zhu
Summary: Adaptive CD4(+) T helper cells and innate lymphoid cells utilize distinct enhancer elements for the induction of T-bet, a critical transcription factor, during their differentiation. IL-18 induces T-bet expression in NK cells through the interaction between RUNX3 and Tbx21-CNS-3, while IL-12 induces T-bet expression in Th1 cells through the binding of STAT to Tbx21-CNS-12.
Article
Agronomy
Carlos Noceda, Augusto Peixe, Birgit Arnholdt-Schmitt
Summary: This study strengthens the selection strategy of reference genes for normalization of gene expression data by integrating analytical revision. Using in vitro olive adventitious rooting as the experimental system, candidate reference genes were ranked according to their transcriptional stability. The results were analytically revised to select the most suitable combination of reference genes. This study provides a general application guide, which is of significant importance for gene expression studies in similar biological systems.
Article
Cell Biology
Jin-Tao Li, Miao Yin, Di Wang, Jian Wang, Ming-Zhu Lei, Ye Zhang, Ying Liu, Lei Zhang, Shao-Wu Zou, Li-Peng Hu, Zhi-Gang Zhang, Yi-Ping Wang, Wen-Yu Wen, Hao-Jie Lu, Zheng-Jun Chen, Dan Su, Qun-Ying Lei
NATURE CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Ziheng Liu, Ying Yang, Aihong Gu, Jiawen Xu, Ying Mao, Haojie Lu, Weiguo Hu, Qun-Ying Lei, Zhouhua Li, Mingjie Zhang, Yu Cai, Wenyu Wen
NATURE COMMUNICATIONS
(2020)
Review
Biochemistry & Molecular Biology
Yi-Ping Wang, Jin-Tao Li, Jia Qu, Miao Yin, Qun-Ying Lei
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Minzhe Zhu, Qianfeng Wang, Pengxiang Tian, Lu Cheng, Zihao Sun, Qin Hong, Pin Lv, Luzhang Ji, Yang Liu, Qi-Qun Tang, Bo Wen
Summary: This study reveals the impact of early embryo-specific lncRNA lncenc1 on glucose and lipid balance in adult mice, with its deficiency leading to a preference for lipid utilization and glucose intolerance. Interestingly, lncenc1-deficient mice on a high-fat diet showed improved health and resistance to food-induced obesity and metabolic disturbances. Additionally, the study suggests that metabolic defects associated with lncenc1 deficiency may already exist in embryos, supporting the concept of fetal origin of adult metabolic disorders.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Letter
Cell Biology
Hui Ming, Qianfeng Wang, Yuwen Zhang, Luzhang Ji, Lu Cheng, Xiangru Huo, Zixiang Yan, Zhexiao Liu, Yongjun Dang, Bo Wen
Article
Biochemistry & Molecular Biology
Kai Li, Jiayue Xu, Yanyun Luo, Dingfeng Zou, Ruiqin Han, Shunshun Zhong, Qing Zhao, Xinyu Mang, Mengzhen Li, Yanmin Si, Yan Lu, Pengyu Li, Cheng Jin, Zhipeng Wang, Fang Wang, Shiying Miao, Bo Wen, Linfang Wang, Yanni Ma, Jia Yu, Wei Song
Summary: This study delineated the global lncRNA expression landscape in mouse spermatogenesis and identified 968 germ cell signature lncRNAs. Functional screening revealed three lncRNAs (Gm4665, 1700027A15Rik, and 1700052122Rik) that directly influence spermatogenesis, with Gm4665 found to occupy the genomic regulatory region of important spermatogenic genes.
Article
Cell Biology
Wei Huang, Na Li, Yi Zhang, Xu Wang, Miao Yin, Qun-Ying Lei
Summary: The study reveals that AHCYL1, as a SAH sensor, inhibits autophagy through PIK3C3, uncovering a new axis of SAH-AHCYL1-PIK3C3, which functions to inhibit autophagy in an MTORC1-independent manner.
Article
Oncology
Qin Hong, Bing Li, Xiumei Cai, Zhengtao Lv, Shilun Cai, Yunshi Zhong, Bo Wen
Summary: The relationship between the transcriptome characteristics and the adenoma-carcinoma sequence in colorectal cancer (CRC) is explored through RNAseq data analysis. Metabolic dysregulation, immune response downregulation, and activation of canonical cancer pathways are identified as key features in CRC formation. TPD52L1 gene is highlighted as a potential marker for tumor formation and poor prognosis in CRC patients.
FRONTIERS IN ONCOLOGY
(2021)
Review
Oncology
Lin Qian, Fan Zhang, Miao Yin, Qunying Lei
Summary: Metabolic remodeling is a key feature in cancer development, leading malignant cells to favor specialized fermentation. Targeted cancer therapies based on reprogramming nutrient or metabolite metabolism have gained substantial attention, as well as the management of nutrient availability becoming more appealing.
CANCER BIOLOGY & MEDICINE
(2022)
Article
Cell Biology
Fan Zhang, Di Wang, Jintao Li, Ying Su, Suling Liu, Qun-Ying Lei, Miao Yin
Summary: Folate metabolism is crucial in tumor development. The enzyme MTHFD2 plays a key role in this process, and its expression is regulated by the deacetylase SIRT4. Understanding this mechanism can impact cancer cell growth.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Developmental Biology
Miao-Lin Li, Si-Yi Cao, Jia Qu, Lei Zhang, Qiang Gao, Xu Wang, Miao Yin, Ying Liu, Ming-Zhu Lei, Qun-Ying Lei
Summary: The study demonstrates the importance of methionine metabolism and its intermediate metabolite SAM in intestinal development and homeostasis. SAM plays a crucial role in maintaining the integrity of differentiated epithelium and protecting intestinal epithelial cells from apoptosis.
Article
Multidisciplinary Sciences
Jingyu Li, Kang Zhu, Aihong Gu, Yiqing Zhang, Shijing Huang, Ronggui Hu, Weiguo Hu, Qun-Ying Lei, Wenyu Wen
Summary: Lipid homeostasis is crucial for cellular functions and its dysregulation is associated with human diseases. This study demonstrates that Spartin promotes the formation of Itch condensates independently of lipid droplets, activates Itch through self-oligomerization, and accelerates the generation of polyubiquitin chains through liquid-liquid phase separation, forming a positive feedback loop. These findings provide important insights into lipid metabolism and autophagic degradation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Editorial Material
Cell Biology
Yifan Zhang, Ming-Zhu Lei, Miao Yin, Qun-Ying Lei
Summary: A recent report suggests that obesity may hinder cancer immunogenicity and promote a tumor microenvironment lacking type I interferon (IFN-I) through STING inhibition mediated by saturated fatty acids.
TRENDS IN CELL BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Qi-Xiang Ma, Wen-Ying Zhu, Xiao-Chen Lu, Duo Jiang, Feng Xu, Jin-Tao Li, Lei Zhang, Ying-Li Wu, Zheng-Jun Chen, Miao Yin, Hai-Yan Huang, Qun-Ying Lei
Summary: This study reveals the critical role of the branched-chain amino acid-branched-chain keto acid axis in the regulation of white adipose tissue browning.
Article
Biochemistry & Molecular Biology
Ming-Zhu Lei, Xu-Xu Li, Ye Zhang, Jin-Tao Li, Fan Zhang, Yi-Ping Wang, Miao Yin, Jia Qu, Qun-Ying Lei
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2020)