Article
Hematology
Haiyun Pei, Huilin Li, Lei Xu, Bowen Zhang, Heng Zhang, Yali Jia, Liqing Liang, Xiaoyan Xie, Zeng Fan, Zhou Yang, Xiaoling Wang, Feiling Song, Lijuan He, Wen Yue, Xuetao Pei
Summary: The major challenge in using human induced pluripotent stem cells (hiPSCs) is their efficient and controlled differentiation into specific cell types. This study focused on understanding the characteristics of hiPSCs derived from human umbilical arterial endothelial cells (HuA-iPSCs) and their differentiation potential. It was found that HuA-iPSCs retained a transcriptional memory and DNA methylation signature similar to their parental cells, and they showed the highest efficiency in differentiating into hematopoietic cells compared to other hiPSCs.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Chiyuan Ma, Yue Xiong, Pei Han, Xueying Zhang, Yujing Cao, Baobei Wang, Huashan Zhao, Enkui Duan, Jian V. Zhang, Xiaohua Lei
Summary: This study demonstrates for the first time the significant impact of microgravity on hematopoietic differentiation of human pluripotent stem cells (hPSCs), and reveals the underlying mechanism for the effect of simulated microgravity on hematopoiesis development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xinchao Miao, Kunimichi Niibe, Maolin Zhang, Zeni Liu, Praphawi Nattasit, Yumi Ohori-Morita, Takashi Nakamura, Xinquan Jiang, Hiroshi Egusa
Summary: Amelogenin is a major component of enamel proteins, but the role of Amelx transcriptional activation in regulating ameloblast differentiation from iPSCs is unclear. This study induced Amelx expression in mouse iPSCs and used a three-stage strategy to differentiate cells into ameloblast lineage. Lithium chloride was found to enhance differentiation of dental epithelial cells, leading to the formation of ameloblast lineage with specific markers and calcium deposition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Vrathasha Vrathasha, Sergei Nikonov, Brent Allen Bell, Jie He, Yajat Bungatavula, Katherine Elizabeth Uyhazi, Venkata Ramana Murthy Chavali
Summary: This study successfully demonstrated the potential of using human-induced pluripotent stem cells to differentiate into functional retinal ganglion cells and transplant them as a replacement therapy for glaucoma. The transplanted cells showed similar morphology and function to native cells, providing key strategies for the efficiency of stem cell replacement therapy in neurodegenerative diseases.
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Kazunori Bando, Hiromi Yamashita, Motomu Tsumori, Hayase Minoura, Koji Okumura, Fumiyuki Hattori
Summary: This study developed an all-in-one automated culture machine that can efficiently expand human pluripotent stem cells and differentiate them into various cell types. This would facilitate the social implementation of human pluripotent stem cells and contribute to the standardization of the entire handling processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinchao Miao, Kunimichi Niibe, Yunyu Fu, Maolin Zhang, Praphawi Nattasit, Yumi Ohori-Morita, Takashi Nakamura, Xinquan Jiang, Hiroshi Egusa
Summary: This study reveals that Epfn activation promotes ameloblast induction from mouse iPSCs by upregulating BMP-Smad activity, suggesting Epfn as a promising target for enamel and tooth regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Vincent P. Willard, Holly A. Leddy, Daniel Palmer, Chia-Lung Wu, Wolfgang Liedtke, Farshid Guilak
Summary: The expression and activation of TRPV4 in chondrogenesis play a crucial role in regulating cartilaginous matrix production through modulation of chondrogenic gene markers and Ca2+ signaling. Understanding the involvement of TRPV4 in cartilage development may lead to new therapeutic strategies for cartilage-related diseases and accelerate stem cell differentiation for tissue engineering purposes.
Article
Multidisciplinary Sciences
Devansh Agarwal, Rian Kuhns, Christos N. N. Dimitriou, Emmalyn Barlow, Karl J. J. Wahlin, Ray A. A. Enke
Summary: Researchers utilized human pluripotent stem cell-derived 3D retinal organoids and Next Generation Sequencing to explore the gene regulation and cell fate determination in human retinal development, providing valuable reference for vision research.
Article
Medicine, Research & Experimental
Wei Shan, Liping Zhou, Lizhen Liu, Deju Lin, Qin Yu
Summary: In this study, iPSCs were reprogrammed from somatic cells and differentiated into neurons through induction, with the key role of Bmi1 in the process highlighted.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Cell & Tissue Engineering
Gulen Guney-Esken, Ozgur Dogus Erol, Burcu Pervin, Gulben Gurhan Sevinc, Tamer Onder, Elif Bilgic, Petek Korkusuz, Aysen Gunel-Ozcan, Duygu Uckan-Cetinkaya, Fatima Aerts-Kaya
Summary: GS-2 is a rare autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene. This study successfully generated iPSC from 3 GS-2 patients using lentiviral vectors, which expressed pluripotency markers and differentiated into cells from all three germ layers. The iPSCs can be used for future studies on novel treatments and the pathophysiology of GS-2 disease.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Ryosuke Negoro, Kanae Kawai, Moe Ichikawa, Sayaka Deguchi, Kazuo Takayama, Hiroyuki Mizuguchi
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Article
Multidisciplinary Sciences
Yukiko Toba, Sayaka Deguchi, Natsumi Mimura, Ayaka Sakamoto, Kazuo Harada, Kazumasa Hirata, Kazuo Takayama, Hiroyuki Mizuguchi
Article
Pharmacology & Pharmacy
Kazuo Takayama, Kohei Ito, Akiko Matsui, Tomoki Yamashita, Kentaro Kawakami, Daisuke Hirayama, Wataru Kishimoto, Hiroshi Nakase, Hiroyuki Mizuguchi
Summary: In this study, gene expression profiles related to drug absorption, distribution, metabolism, and excretion were analyzed in biopsy samples from different regions of the intestine of patients with inflammatory bowel disease. The study found significant differences in gene expression levels across different regions of the intestine, particularly in drug-metabolizing enzymes and drug transporters. This information may be crucial for accurately predicting the pharmacokinetics of orally administered drugs.
DRUG METABOLISM AND DISPOSITION
(2021)
Article
Immunology
Kahori Shimizu, Fuminori Sakurai, Shunsuke Iizuka, Ryosuke Ono, Tomohito Tsukamoto, Fumitaka Nishimae, Shin-Ichiro Nakamura, Toru Nishinaka, Tomoyuki Terada, Yasushi Fujio, Hiroyuki Mizuguchi
Summary: Adenovirus vectors can cause hepatotoxicity in two phases post-administration, with early phase involving inflammatory cytokines. The modified Ad vector, Ad-E4-122aT-CAL2, significantly suppresses leaky Ad gene expression in the liver, leading to reduced acute hepatotoxicity. IL-6 induced by Ad vectors enhances leaky Ad gene expression and cytotoxicity, but this effect is attenuated by the presence of Ad-E4-122aT-CAL2.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Moe Ichikawa, Ryosuke Negoro, Kanae Kawai, Tomoki Yamashita, Kazuo Takayama, Hiroyuki Mizuguchi
Summary: The study demonstrated that vinblastine treatment can effectively remove residual undifferentiated hiPSCs in hiPSC-IECs, leading to decreased pluripotent marker expression and increased intestinal marker expression. The viability of undifferentiated hiPSCs was significantly reduced by vinblastine treatment. MDR1-negative cells, such as undifferentiated hiPSCs, were found to die upon vinblastine treatment due to their inability to excrete the drug, suggesting that vinblastine-treated hiPSC-IECs could be a safe and effective cell source for regenerative medicine.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Oncology
Ryosuke Ono, Kosuke Takayama, Fuminori Sakurai, Hiroyuki Mizuguchi
Summary: The study developed a novel OAd fully composed of OAd35, which recognizes CD46 as an infection receptor and showed efficient cell lysis activities similar to OAd5 in CAR-positive tumor cells, while exhibiting higher levels of cell lysis activities than OAd5 in CAR-negative tumor cells. OAd35 had lower proportions of neutralizing antibodies in adults compared to Ad5.
MOLECULAR THERAPY-ONCOLYTICS
(2021)
Article
Oncology
Fuminori Sakurai, Fumitaka Nishimae, Kosuke Takayama, Hiroyuki Mizuguchi
Summary: This study examined five types of oncolytic adenoviruses (OAds) containing different types of E1 gene expression cassettes, and found that OAd-Delta 24, with a 24-bp deletion in the E1A gene, demonstrated the most efficient oncolytic activities against human tumor cell lines, despite slightly higher cytotoxicity to normal human cells compared to other OAds. These results provide important insights for the development of safe and effective OAds.
ANTICANCER RESEARCH
(2021)
Article
Virology
Chieko Inoue, Ryosuke Negoro, Kazuo Takayama, Hiroyuki Mizuguchi, Fuminori Sakurai
Summary: This study demonstrates that hiPS-SIECs are a promising in vitro model for evaluating virus infection, with differences observed in replication and immune responses of reovirus in these cells under different infection methods.
Article
Chemistry, Analytical
Menglu Li, Yukiko Ueyama-Toba, Matthew Lindley, Gunganist Kongklad, Yasunori Nawa, Yasuaki Kumamoto, Seiichi Ishida, Yasunari Kanda, Satoshi Fujita, Hiroyuki Mizuguchi, Katsumasa Fujita
Summary: To promote the clinical application of hiPSC-derived hepatocytes, a label-free Raman microscopy method is used to monitor regenerative processes and assess differentiation efficiency. The method has successfully identified intracellular biomolecules in live samples and compared the phenotypes of hiPSC-derived hepatocytes with HepaRG and commercially available iCell hepatocytes. The study also explored the use of Raman imaging as a hepatotoxicity assay, demonstrating its potential for quality control and hepatotoxicity screening.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jumpei Inui, Yukiko Ueyama-Toba, Seiji Mitani, Hiroyuki Mizuguchi
Summary: A method has been developed to dissociate, cryopreserve, and reseed human induced pluripotent stem cell-derived hepatocyte-like cells (HLCs) without loss of their functions. This technology enables the availability of cryopreserved HLCs for drug discovery research.
Article
Biology
Eiko Sakai, Tsutomu Imaizumi, Ruruka Suzuki, Marcos Taracena-Gandara, Toshiki Fujimoto, Fuminori Sakurai, Hiroyuki Mizuguchi
Summary: Non-alcoholic liver disease (NAFLD) is caused by excessive fat accumulation in the liver and miR-27b has been found to play a crucial role in NAFLD development by targeting genes involved in lipid metabolism. This study identified MAIP1 as a direct target of miR-27b and showed that overexpression of MAIP1 can ameliorate lipid accumulation induced by miR-27b. Additionally, decreased Maip1 expression was observed in mice fed a high-fat diet, suggesting its involvement in fatty liver condition and its potential as a therapeutic target for NAFLD.
COMMUNICATIONS BIOLOGY
(2023)
