Chemiluminescence flow sensor with immobilized reagent for the determination of pyrogallol based on potassium hexacyanoferrate(III) oxidation

A novel chemiluminescence (CL) flow-through sensor for the determination of pyrogallol has been developed. The method is based on the reaction between pyrogallol and potassium hexacyanoferrate(III) in sodium hydroxide solution. Potassium hexacyanoferrate(III) involved in the CL reaction was electrostatically immobilized on anion-exchange resin packed in a column. Pyrogallol was sensed by the CL reaction between pyrogallol and potassium hexacyanoferrate(III) which was eluted from the ion-exchange column through sodium phosphate injection. The CL emission allows quantitation of pyrogallol concentration in the range 0.01-3.8 mu g/mL with a detection limit (3 sigma) of 0.003 mu g/mL and a sample throughput of 118 h(-1). The relative standard deviation (n = 7) was 2.2% for 0.2 mu g/mL of pyrogallol. The influence of foreign compounds was tested. (C) 2008 Elsevier B.V. All rights reserved.