Article
Biology
Filippo Migliorini, Hanno Schenker, Nicola Maffulli, Frank Hildebrand, Jorg Eschweiler
Summary: The present study investigated the osseointegration promoted by functionalised ceramics with peptide Arg-Gly-Asp (RGD) in a rabbit model in vivo. The overall osteoid and bone implant contact improved significantly from 6 to 12 weeks. Finally, RGD enhanced ceramic promoted faster osteoid implant contact in vivo than titanium implants.
Article
Oncology
Elia Bari, Massimo Serra, Mayra Paolillo, Eric Bernardi, Sara Tengattini, Filippo Piccinini, Cristina Lanni, Marzio Sorlini, Giovanni Bisbano, Enrica Calleri, Maria Luisa Torre, Sara Perteghella
Summary: The paper discusses the active targeting of tumor cells using integrin-targeted nanoparticles, specifically using silk fibroin nanoparticles (SFNs) functionalized with RGD. This strategy effectively boosts antitumor activity and reduces damage to healthy organs by actively internalizing into tumor cells overexpressing integrin receptors. The functionalization of SFNs with cRGD provides in vitro active targeting for site-specific delivery of anticancer drugs, showing increased uptake by tumor cells and potential for improving anticancer therapies.
Article
Chemistry, Multidisciplinary
Tetsuo Asakura, Akira Naito
Summary: This study aims to develop an excellent wound dressing material by introducing the three-residue sequence of the fibronectin adhesion site into silk fibroin. The results show that the high-functionalized silk fibroin has the potential to play a significant role in the field of wound dressings, as demonstrated both in vitro and in vivo.
Article
Materials Science, Ceramics
Zhengwei Xu, Xiaohong Jiang
Summary: In this research, TiO2 nano-porous arrays modified with polydopamine (PDA) and Arg-Gly-Asp (RGD) peptide were investigated for their potential application in implantable materials. The modified arrays were found to enhance biomineralization and exhibit excellent osteogenic performance, as confirmed by various characterization techniques and in vitro experiments. These findings highlight the great potential of such modified TiO2 nano-porous arrays in the field of biomineralization and osteogenesis.
CERAMICS INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Tomoko Hashimoto, Yuka Nakamura, Sachiro Kakinoki, Naoko Sano, Tsunenori Kameda, Yasushi Tamada, Tetsuji Yamaoka, Hiromichi Kurosu
Summary: This study proposes a simple method to immobilize the functional peptide of GRGDS on the surface of SF films using GAGAGS sequences. This method does not involve any chemical reaction, preserving the original properties of SF molecules. The density of GRGDS peptides on GAGAGS-bound SF films was found to be higher compared to non-GAGAGS-bound films. Furthermore, the amount of immobilized (GAGAGS)GRGDS peptide increased with the promotion of β-sheet crystallization in the SF film. Fibroblasts adhered to the SF film showed increased extensibility due to (GAGAGS)GRGDS immobilization, indicating effective cell adhesion activity of RGD.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Xinyu Xu, Hao Wang, Shuling Zhang, Xiaohan Mei, Boda Ying, Ruiyan Li, Yanguo Qin
Summary: This study fabricated orthogonal porous PEI scaffolds using fused deposition modeling (FDM) 3D printing method and constructed an osteogenic microenvironment on the scaffold. The PEI/PDA/RGD scaffold exhibited desirable mechanical properties, hydrophilicity, and biocompatibility. It promoted the osteogenic differentiation of MC3T3-E1 osteoblasts by upregulating the expression of osteogenic genes. The PEI/PDA/RGD scaffold holds promise as a bone biomaterial for bone defect repair.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Materials Science, Biomaterials
Lichao Chen, Bo Wang, Haohao Ren, Yanan Wu, Defu Lyu, Yanan Ouyang, Qiyi Zhang, Yonggang Yan
Summary: This study designed and fabricated a poly-amino acid/poly (p-benzamide) (PAA-PBA) copolymer with high mechanical strength, which can address the limitations of PAA in orthopedics. By surface modification, the bioactivity of the material was significantly improved. Functionalized samples demonstrated improved ability to promote bone mineralization, cell adhesion, proliferation, and osteogenic differentiation.
BIOMATERIALS ADVANCES
(2022)
Article
Biophysics
Khalid Odamizil Alzamely, Farnaz Hajizadeh, Morteza Heydari, Mohamad Javad Ghaderi Sede, Sima Hydarzadeh Asl, Makwan Peydaveisi, Ali Masjedi, Sepideh Izadi, Afshin Nikkhoo, Fatemeh Atyabi, Afshin Namdar, Behzad Baradaran, Mozhdeh Sojoodi, Farhad Jadidi-Niaragh
Summary: In this study, the inhibition of ZEB-1 and CD73 expression in tumor sites using RGD-CL nanoparticles significantly reduced cancer cell migration and proliferation, leading to tumor suppression and prolonged survival in tumor-bearing mice. This highlights the importance of targeting the CD73/ZEB1 axis in cancer cells for potential future use in cancer patients.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Cell Biology
Kensuke Kitsugi, Hidenao Noritake, Moe Matsumoto, Tomohiko Hanaoka, Masahiro Umemura, Maho Yamashita, Shingo Takatori, Jun Ito, Kazuyoshi Ohta, Takeshi Chida, Barbara Ulmasov, Brent A. Neuschwander-Tetri, Takafumi Suda, Kazuhito Kawata
Summary: This study found that CWHM12 suppresses the proliferation of activated HSCs and promotes apoptosis by blocking the Hippo signaling pathway. Furthermore, CWHM12 induces cell cycle arrest at the G1 phase. The inhibition of focal adhesion kinase (FAK) phosphorylation leads to the translocation of Yes-associated protein (YAP). These findings provide a novel drug target for the treatment of hepatic fibrosis.
CELLULAR SIGNALLING
(2022)
Article
Biochemistry & Molecular Biology
Zhongqiang Qiu, Aileen Park, Lianzijun Wang, Rachel Wilsey, Myeongwoo Lee
Summary: UNC-52/perlecan is a basement membrane proteoglycan essential for muscle cell attachment in C. elegans. Mutations in the RGD motif of UNC-52/perlecan affect actin filaments and integrin localization, leading to developmental defects and paralysis. The RGD motif is a potential conserved cell-binding motif in invertebrates.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Shuangling Chen, Meng Liang, Chengli Wu, Xiaoyi Zhang, Yuji Wang, Ming Zhao
Summary: In order to mitigate the bleeding side effects during thrombolytic therapy, a novel biodegradable nanosystem was developed to deliver drugs specifically to the thrombus. The UK/PD-RGDS complex nano-globule showed 1.3 times higher thrombolytic potency compared to commercial UK. In vivo experiments demonstrated that the UK/PD-RGDS complex could achieve equivalent thrombolysis with a 90% reduction in UK dosage.
Article
Materials Science, Multidisciplinary
Ximing Chen, Yan Ma, Wenwei Li, Qingwen Gui, Xuhua Zhan, Yaqin Lan, Weisong Pan, Tiean Zhou, Zhaohong Su
Summary: This study presents a functional coating with cell-specific adhesion and antiprotein adhesion properties prepared by electrochemical copolymerization, showing significantly improved cell adsorption and antiprotein adhesion characteristics.
ACS APPLIED POLYMER MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Olivier Schussler, Pierre E. Falcoz, Juan C. Chachques, Marco Alifano, Yves Lecarpentier
Summary: The clinical impact of cell therapy after myocardial infarction is limited by low cell engraftment, cell death in inflammatory and poor angiogenic areas, and secondary migration. Enhancing integrin interaction with cells through a three-dimensional material, especially when applied epicardially, may improve outcomes. Using collagen as a polymer backbone for tissue engineering shows promise in creating contractile tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Kensuke Kitsugi, Hidenao Noritake, Moe Matsumoto, Tomohiko Hanaoka, Masahiro Umemura, Maho Yamashita, Shingo Takatori, Jun Ito, Kazuyoshi Ohta, Takeshi Chida, Barbara Ulmasov, Brent A. Neuschwander-Tetri, Takafumi Suda, Kazuhito Kawata
Summary: CWHM12 inhibits cellular senescence in HSCs by disrupting RGD-mediated integrin binding.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jing Yu, Kai Xu, Xiaojing Chen, Xiaodan Zhao, Yuxuan Yang, Dake Chu, Yongliang Xu, Qiang Zhang, Yanfeng Zhang, Yilong Cheng
Summary: In this study, an advanced hydrogel was prepared through one-pot free-radical copolymerization, demonstrating excellent mechanical properties and biocompatibility, with vast potential in biomedical and flexible electronic applications.
Article
Chemistry, Organic
Qiuxin Zhang, Weiyi Tan, Bing Xu
Summary: In this study, we synthesized pyrrole-conjugated phosphopeptides and investigated their effects on cell viability. The results showed that the introduction of a heteroaromatic motif at the N-terminal of short peptides reduced their inhibitory activity against cancer cells and hindered morphological changes during enzymatic dephosphorylation.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Jialei Song, Chengling Wu, Yan Zhao, Min Yang, Qingxin Yao, Yuan Gao
Summary: A bioorthogonal disassembly strategy was developed to prompt clearance of supramolecular assemblies within living cells. The enzyme-instructed self-assembly process led to the disruption of pi-pi stacking and induced disassembly of the assemblies, depriving them of potency and addressing their unintended retention and cellular toxicity. This strategy leverages biosafety in developing nanomaterials for drug delivery systems.
Article
Biochemical Research Methods
Weiyi Tan, Qiuxin Zhang, Pengyu Hong, Bing Xu
Summary: This study utilizes an enzyme-instructed self-assembling thiophosphopeptide as a probe to reveal the state of the Golgi Apparatus (GA) in live cells. Results show that the probe accumulates in the GA at different rates depending on cell types and is sensitive to alkaline phosphatase expression. This self-assembling probe offers a novel approach for high-resolution imaging of subcellular organelles.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Weiyi Tan, Qiuxin Zhang, Monica C. Quinones-Frias, Alan Y. Hsu, Yichi Zhang, Avital Rodal, Pengyu Hong, Hongbo R. Luo, Bing Xu
Summary: This study demonstrates the effectiveness of unconventional peptide thioesters in selectively targeting the Golgi apparatus, thereby controlling cell fate. These peptide thioesters enter cells through specific endocytic pathways and accumulate in the Golgi apparatus, disrupting protein trafficking and leading to cell death.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Chemistry, Multidisciplinary
Qiuxin Zhang, Weiyi Tan, Bing Xu
Summary: Enzymatic noncovalent synthesis (ENS) is a mechanism that utilizes enzymatic reactions to produce spatially organized higher-order supramolecular assemblies, which can modulate cellular processes. This Review highlights the recent progress in the application of ENS for targeted subcellular organelles, showcasing its potential in understanding diseases, controlling cell behaviors, and developing new therapeutics.
Article
Chemistry, Multidisciplinary
Qiuyu Lu, Yaxin Hu, Cheuk Yin Li, Yi Kuang
Summary: This study introduces a readout control module that reduces protein leakage in synthetic mRNA switches, thereby decreasing signal noise and increasing input sensitivity and output range. These switches are applicable to different types of input molecules and output proteins, offering potential for accurate cell manipulation in biomedical applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Meihui Yi, Fengbin Wang, Weiyi Tan, Jer-Tsong Hsieh, Edward H. Egelman, Bing Xu
Summary: This study demonstrates the use of enzymatic responsive rigid-rod aromatics as substrates to target undruggable phosphatases and kill cancer cells in a bone microenvironment. The compounds effectively kill metastatic prostate cancer cells and mimic osteoblast cells, with high cellular uptake rate.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Nanoscience & Nanotechnology
Jiali Chen, Pai Zhang, Chengling Wu, Qingxin Yao, Ruitao Cha, Yuan Gao
Summary: This article presents a novel nitroreductase (NTR) labile peptidic hydrogel based on supramolecular self-assembly for the delivery of probiotics to the intestinal tract. The hydrogel effectively protects probiotics against harsh gastrointestinal conditions, and can be triggered to release probiotics by upregulated NTR in the intestine. The probiotic-loaded hydrogel shows enhanced therapeutic efficacy in an ulcerative colitis model by reducing proinflammatory cytokines and promoting intestinal barrier repair. Additionally, the hydrogel remodels the gut microbiome by increasing the diversity and abundance of indigenous probiotics, contributing to improved therapies for inflammatory bowel diseases.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Jie Qi, Chen Hang, Yuan Gao, Xingyu Jiang
Summary: Highbiocompatibility and stability against water and scratch are essential for liquid metal-based flexible electronics. In this study, we synthesized polydopamine-coated liquid metal nanoparticles (LMNPs) using a controllable and scalable thermal processing method. The PD@LM ink enabled high-resolution printing on various substrates, and the printed circuit showed high stability against repeated stretch in water and scratch. The PD@LM electrode demonstrated high biocompatibility, conductivity, and stretchability, making it suitable for applications such as recording changes in membrane potential and detecting electrocardiogram signals in vivo.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Cheuk Yin Li, Zhenghua Liang, Lejian Liu, Yi Kuang
Summary: In this study, a novel approach was designed using synthetic mRNA switches to convey intracellular marker molecule information into spatially controlled extracellular toxic assembly formation. This approach ensures high target cell recovery effectiveness and high non-target cell elimination effectiveness by bypassing the use of toxic protein and amplifying the marker molecule information at multiple levels. It can be tailored to meet various in situ cell purification needs, promising high-quality in situ cell purification for a wide range of biomedical applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Kaixin Tan, Yaxin Hu, Zhenghua Liang, Cheuk Yin Li, Wai Laam Yau, Yi Kuang
Summary: Synthetic mRNA circuits control output protein expression by detecting specific input molecules in cells. Existing circuits either enhance or repress protein production upon input binding, but this binary mechanism limits precise control. In this study, bidirectional mRNA circuits were designed to independently control enhancer and repressor modules using different input molecules, resulting in fine-tuning of protein expression. This research expands the capabilities of synthetic mRNA circuits for precise cell manipulation and provides cell-based solutions to biomedical problems.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Adrianna N. Shy, Jiashu Xu, Beom Jin Kim, Bing Xu
Summary: This study focuses on the enzyme-instructed self-assembly (EISA) of a phosphopeptide (1P) derived from the protein crystal structures of Merlin and CRL4(DCAF-1). The results demonstrate that even with the presence of a hydrophilic peptide, 1P can form a hydrogel at a very low volume fraction. Additionally, diastereomer 2P and enantiomer 3P of 1P are able to form hydrogels via EISA at higher concentrations than 1P.
Article
Biochemical Research Methods
Qingxin Yao, Feng Lin, Chenghao Lu, Ruijia Zhang, Hanlin Xu, Xiaoqian Hu, Ziyang Wu, Yuan Gao, Peng R. Chen
Summary: This article introduces a dual-mechanism targeted bioorthogonal prodrug therapy that utilizes two orthogonal, receptor-independent tumor-targeting strategies to maximize therapeutic efficacy and minimize adverse drug reactions for anticancer therapies.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Yan Zhao, Qingxin Yao, Jiali Chen, Ruijia Zhang, Jialei Song, Yuan Gao
Summary: There is an urgent need for a visual drug delivery system in precision medicine. This study constructs a self-reporting bioorthogonal prodrug activation system that interprets prodrug activation events using fluorescence emission. The system selectively forms in tumor cells and can reflect the release of active drugs through measuring fluorescence brightness.
BIOMATERIALS SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Huyeon Choi, Gaeun Park, Eunhye Shin, Seon Woo Shin, Batakrishna Jana, Seongeon Jin, Sangpil Kim, Huaimin Wang, Sang Kyu Kwak, Bing Xu, Ja-Hyoung Ryu
Summary: Mitochondria-targeting nucleopeptide can sequester ATP by self-assembling, leading to apoptosis of cancer cells.
Article
Chemistry, Physical
Yiping Yin, Zhe Wang, Hua Zou
Summary: This study presents a novel method for preparing dimpled polymer-silica nanocomposite particles using interfacial swelling-based seeded polymerization. The optimized conditions allow for a relatively high percentage of dimpled particles to be achieved.
Article
Chemistry, Physical
Brenden D. Hoehn, Elizabeth A. Kellstedt, Marc A. Hillmyer
Summary: Porous materials with nanometer-scale pores have important applications as nanoporous membranes. In this study, ABA triblock copolymers were used as precursors to produce nanoporous polymeric membranes (NPMs) in thin film form by degrading the end blocks. Polycyclooctene (PCOE) NPMs with tunable pore sizes were successfully prepared using solvent casting technique. Oxygen plasma etching was employed to improve the surface porosity and hydrophilicity of the membranes. This study provides a straightforward method to produce tough NPMs with high porosity and hydrophilic surface properties.
Article
Chemistry, Physical
Vladislav S. Petrovskii, Stepan I. Zholudev, Igor I. Potemkin
Summary: This article investigates the behavior of linear and ring polypeptide chains in aqueous solution and explores the properties of the complexes formed by these chains with oppositely charged surfactants. The results demonstrate that the complexes of linear supercharged unfolded polypeptides and the corresponding surfactants exhibit impressive adhesive properties.
Article
Chemistry, Physical
Merve Cevik, Serkan Dikici
Summary: Cardiovascular diseases are a leading cause of death globally, and vascular grafts are a promising treatment option. This study focuses on tissue-engineered vascular grafts (TEVGs) using decellularized parsley stems as a potential biomaterial. The decellularized parsley stems showed suitable properties for TEVGs, providing a suitable environment for human endothelial cells to form a pseudo endothelium. This study showcases the potential of using parsley stems for TEVGs.
Article
Chemistry, Physical
Gustavo A. Vasquez-Montoya, Tadej Emersic, Noe Atzin, Antonio Tavera-Vazquez, Ali Mozaffari, Rui Zhang, Orlando Guzman, Alexey Snezhko, Paul F. Nealey, Juan J. de Pablo
Summary: The optical properties of liquid crystals are typically controlled by electric fields. In this study, we investigate the effects of microfluidic flows and acoustic fields on the molecular orientation and optical response of nematic liquid crystals. We identify several previously unknown structures and explain them through calculations and simulations. These findings hold promise for the development of new systems combining sound, flow, and confinement.
Article
Chemistry, Physical
Xinjun Wu, Xin Guan, Shushu Chen, Jiangpeng Jia, Chongyi Chen, Jiawei Zhang, Chuanzhuang Zhao
Summary: This research presents a novel shape memory hydrogel with a remodelable permanent shape and programmable cold-induced shape recovery behavior. The hydrogel is prepared using specific treatment methods to achieve shape fixation by heating and shape recovery by cooling. Additionally, deformable devices can be obtained by assembling hydrogel blocks with different concentrations.
Article
Chemistry, Physical
Rebecca Hengsbach, Gerhard Fink, Ulrich Simon
Summary: This study examines the properties of DNA functionalized pNipmam microgels and pure pNipmam microgels at different concentrations of sodium chloride and in PBS solutions using temperature dependent H-1-NMR measurements. The results show that DNA modification affects the volume phase transition temperature and the addition of salt and PBS further enhances this effect.
Article
Chemistry, Physical
Ningyi Li, Junhong Li, Lijingting Qing, Shicheng Ma, Yao Li, Baohui Li
Summary: This paper investigates the self-assembly behavior of colloids with competing interactions under spherical confinement and finds that different ordered structures can be formed under different sized spherical confinements. Moreover, more perforated structures are formed in smaller spheres.
