Article
Materials Science, Multidisciplinary
Yongling Ding, Xiaofeng Tian, Jinglou Zhang, Mingchen Zhang, Chunrong Ren, Kangning Sun, Weili Wang, Huadong Sun, Laixue Pang
Summary: A novel strategy for the synthesis of double molecular recognition functionalized magnetic graphene nanocomposites was proposed to selectively adsorb glycoproteins in a neutral environment. The composite exhibited high adsorption capacity and rapid separation, with excellent specificity towards glycoproteins. The results suggest great potential for separating and enriching glycoproteins from complex biological samples for analysis.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Materials Science, Multidisciplinary
Zhiyu Li, Yichun Gao, Peng An, Fang Lan, Yao Wu
Summary: In this study, a systematic integration of enrichment and comparative analysis strategy using hydrophilic magnetic covalent organic frameworks (COFs) was developed to successfully enrich large amounts of glycoproteins from colorectal cancer cells. The differential glycoproteins identified through comparative analysis have potential as biomarkers and therapeutic targets.
MATERIALS & DESIGN
(2022)
Article
Biochemical Research Methods
Huan Huang, Qiong Zheng, Yanting He, Chao Zhong, Wenchang Tian, Shasha Zhang, Juan Lin, Zian Lin
Summary: This study introduces a new bifunctional polymer monolithic column for tunable and specific enrichment of glycoproteins and phosphoproteins. The poly (EDMA-co-VPBA-co-VPA) monolith exhibited superior performance in selective separation of small molecules and biomacromolecules containing cis-diol/phosphate groups, with higher binding capacity towards glycoproteins and phosphoproteins compared to non-glycosylated/phosphorylated proteins. The successful application in specific enrichment of glycopeptides/phosphopeptides from complex samples highlights the practicality of this approach in post-translational modification-proteomics development.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Nanoscience & Nanotechnology
Xianglian Liu, Xuan Chen, Yangchao Dong, Chuanyu Zhang, Xiaoli Qu, Yingfeng Lei, Zhuangde Jiang, Xueyong Wei
Summary: The overlapping epidemiology and clinical manifestations of flaviviruses make the differential diagnosis of these viral diseases complicated and unreliable. We developed a sorting system for the early-stage differential diagnosis of dengue and tick-borne encephalitis. By using aptamer-modified polystyrene microspheres, we were able to specifically capture the viruses and sort them according to their size using a traveling surface acoustic wave device. The results showed that the acoustic sorting process was effective and damage-free for subsequent analysis, and this strategy could be used for sample pretreatment in the differential diagnosis of viral diseases.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Environmental Sciences
Zhiyang Lin, Linhan Li, Kaige Song, Bin Yang, Guoqing Zhou, Gengrong Zhang, Jiang Teng, Erlong Wang, Xiaoqiang Liu, Fei Ling, Gaoxue Wang, Tianqiang Liu
Summary: Viruses pose a significant threat to humans when present in contaminated waters, especially for vulnerable populations. In this study, bench-scale experiments were conducted to evaluate the removal of a model virus (SVCV) in water using boronic acid-modified bacterial cellulose microspheres as adsorbents. The results showed that SVCV was effectively removed with a 3-log reduction in virus concentration within 3 hours. This research provides substantial evidence that bacterial cellulose-based materials have the potential for virus removal from water, which is of great importance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Efi Makdasi, Yafit Atiya-Nasagi, David Gur, Itai Glinert, Shlomo Shmaya, Elad Milrot, Theodor Chitlaru, Emanuelle Mamroud, Orly Laskar, Ofir Schuster
Summary: This study evaluated the growth rates of Francisella tularensis strains in iron-enriched blood cultures to improve and accelerate bacterial growth. Shortening the culture time can facilitate rapid pathogen detection and isolation, positively impacting clinical diagnosis and enabling efficient therapy.
MICROBIOLOGY SPECTRUM
(2022)
Article
Engineering, Electrical & Electronic
Bing Sun, Xinjiang Wang, Xiaobo Ma, Zhongyuan Sun, Zhiqiang Wang, Zuxing Zhang, Kaiming Zhou
Summary: Magnetic-based polydimethylsiloxane microspheres (MPMs) are fabricated by incorporating uniformly sized Mn3O4 nanocrystals into polydimethylsiloxane (PDMS) matrix. The MPMs act as optical resonators and can measure the magnetic field by detecting the shift in the resonant wavelength. The sensitivity and measurement range of the MPMs have been optimized, achieving high sensitivity and a wide measurement range. Additionally, the MPMs exhibit excellent long-term stability.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Chemistry, Physical
Helin Xu, Xueqin Li, Yanyan Hao, Xiaoqing Xu, Yan Zhang, Jinli Zhang
Summary: Acteoside (ACT) is a bioactive component with potential applications in various fields. The study proposed a novel strategy to synthesize polymer microspheres modified with polyethyleneimine (PEI) for efficient adsorption of ACT, showing increased adsorption capacity and selectivity through PEI introduction.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Physical
Yueqi Zhang, Bin Du, Yuting Wu, Zhiwei Liu, Jiang Wang, Jianjie Xu, Zhaoyang Tong, Xihui Mu, Bing Liu
Summary: This study presents a self-made magnetic beads that can rapidly enrich various bacteria in liquid phase through electrostatic attraction. The method has a high adsorption efficiency and allows for simple and rapid enrichment of microorganisms in resource-limited settings.
Article
Biochemistry & Molecular Biology
Vesna Marjanovic, Radmila Markovic, Mirjana Steharnik, Silvana Dimitrijevic, Aleksandar D. Marinkovic, Aleksandra Peric-Grujic, Maja Dolic
Summary: Highly porous lignin-based microspheres modified with magnetite nanoparticles were used for the first time to remove Se(VI) selenate anions from water samples. The research investigated the influence of experimental conditions on the adsorption capacity and analyzed the structural and morphological properties of the adsorbent. The adsorbent showed high affinity for Se(VI) oxyanion but limited potential for multi-cycle material application, making it suitable as a Se fertilizer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Arashdeep Kaur, Phillip L. van der Peet, Janice W-Y Mui, Marion Herisse, Sacha Pidot, Spencer J. Williams
Summary: Sulfoglycolysis pathways allow for the breakdown and recycling of sulfoquinovose in both carbon and sulfur content. Through enrichment cultures, two gram-positive Arthrobacter spp. were discovered to produce sulfolactate as the end-product of their metabolism. Genome sequences revealed a modified sulfoglycolytic EMP gene cluster in these bacteria, expanding our understanding of the sulfo-EMP pathway to include soil bacteria.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
He Qi, Liyan Jiang, Qiong Jia
Summary: The combination of magnetic solid phase extraction (MSPE) technology with magnetic adsorbents for the separation and enrichment of glycoproteins and glycopeptides in human body fluids provides technical support for screening disease biomarkers. The widespread application of magnetic adsorbents enables the quantitative analysis of low-abundance glycoproteins and glycopeptides.
CHINESE CHEMICAL LETTERS
(2021)
Article
Nanoscience & Nanotechnology
Xinling Xie, Hongliang Gao, Xuan Luo, Youquan Zhang, Zuzeng Qin, Hongbing Ji
Summary: Magnetic starch microspheres and methyl methacrylate magnetic starch microspheres were prepared using inverse emulsion polymerization and modified with polyethyleneimine, showing good adsorption performance and thermal stability.
ADVANCED COMPOSITES AND HYBRID MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Baoyue Zhang, Yukui Tong, Jianghua He, Baodong Sun, Feng Zhang, Miaomiao Tian
Summary: A novel adsorbent material was successfully developed for the rapid identification and selective separation of LTL, which has been applied successfully in the detection of LTL in peanut shell samples.
Article
Chemistry, Multidisciplinary
Shujuan Ma, Ruizhi Tang, Yang Yu, Luwei Zhang, Haiyang Zhang, Wenyong Ding, Junjie Ou
Summary: A novel Ti4+-immobilized microsphere was developed as a reusable IMAC sorbent, demonstrating excellent enrichment capability and prolonged lifespan. Its superior performance in phosphopeptide enrichment suggests promising prospects for future commercialization.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Lukas Uhrik, Lenka Hernychova, Petr Muller, Umesh Kalathiya, Malgorzata M. Lisowska, Mikolaj Kocikowski, Maciej Parys, Jakub Faktor, Marta Nekulova, Chris Nortcliffe, Pavlina Zatloukalova, Barbara Ruetgen, Robin Fahraeus, Kathryn L. Ball, David J. Argyle, Borivoj Vojtesek, Ted R. Hupp
Summary: A comparative canine-human therapeutics model is being developed by generating a murine monoclonal antibody specific for canine CD20. Mass spectrometry identified the predominant light chain-7 in full-length IgG, and hydrogen deuterium exchange mass spectrometry highlighted the dominant CDR region implicated in CD20 antigen binding. Gene synthesis created a recombinant murine-canine chimeric monoclonal antibody expressing light chain-7, focusing on CDR3 (V-H) for potential canine lymphoma therapy.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Martina Zahradnikova, Ivana Ihnatova, Erika Lattova, Lukas Uhrik, Eliska Stuchlikova, Rudolf Nenutil, Dalibor Valik, Monika Nalezinska, Josef Chovanec, Zbynek Zdrahal, Borivoj Vojtesek, Lenka Hernychova, Milos Novotny
Summary: Investigating N-glycans in non-mucinous ovarian cancer patients may identify potential markers associated with resistance to platinum/taxane chemotherapy. Detection sensitivity to chemotherapy is crucial for these patients.
JOURNAL OF PROTEOMICS
(2021)
Article
Chemistry, Physical
Daniel Horak, Karolina Turnovcova, Zdenek Plichta, Dana Marekova, Vladimir Proks, Radek Kaiser, David Netuka, Pavla Jendelova
Summary: In this study, a block copolymer consisting of PHP and PMAH was synthesized and conjugated with Dox and/or RGDS targeting peptide to create nanoparticles that can reduce glioblastoma tumor growth. The nanoparticles were found to effectively decrease tumor size and induce more apoptosis in treated cells compared to untreated cells.
COLLOID AND POLYMER SCIENCE
(2022)
Article
Materials Science, Biomaterials
Hana Mackova, Helena Hlidkova, Zhansaya Kaberova, Vladimir Proks, Jan Kucka, Vitalii Patsula, Miroslav Vetrik, Olga Janouskova, Bohumila Podhorska, Ognen Pop-Georgievski, Sarka Kubinova, Daniel Horak
Summary: Research on degradable hydrogel polymeric materials with high water content and tissue-like mechanical properties is essential for drug delivery systems, tissue engineering, medical devices, and biomedical-healthcare sensors. The study introduced hydrogels based on P(HEMA-ATEMA-MPC) with varying degrees of crosslinking, demonstrating that higher crosslinking led to better mechanical properties and slowed degradation.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Multidisciplinary Sciences
Viktoriia Oleksa, Hana Mackova, Hana Engstova, Vitalii Patsula, Oleksandr Shapoval, Nadiia Velychkivska, Petr Jezek, Daniel Horak
Summary: The study focuses on developing upconverting luminescent lanthanide-doped nanoparticles with polymer coatings of different charges as labels for carcinoma cells. The nanoparticles were found to be biocompatible and capable of penetrating cells, showing potential for application in photodynamic therapy. This research highlights the promising prospects of using these particles for cancer treatment.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Darja Lisjak, Masa Vozlic, Uliana Kostiv, Daniel Horak, Boris Majaron, Slavko Kralj, Irena Zajc, Lovro Ziberna, Maja Ponikvar-Svet
Summary: The increasing interest in upconverting nanoparticles (UCNPs) in biodiagnostics and therapy has led to the development of biocompatible UCNPs platforms. This study investigated the effect of different phosphonate coatings on the chemical stability and cytotoxicity of Yb3+, Er3+-codoped NaYF4 UCNPs. The results showed that phosphonate coatings prepared at 80 degrees C significantly improved the chemical stability of UCNPs and reduced their cytotoxicity.
METHODS AND APPLICATIONS IN FLUORESCENCE
(2022)
Article
Nanoscience & Nanotechnology
Oleksandr Shapoval, Hana Engstova, Daniel Jirak, Jan Drahokoupil, Katerina Sulkova, Zuzana Berkova, Ognen Pop-Georgievski, Blanka Holendova, Petr Jezek, Daniel Horak
Summary: Novel Yb, Tb, Nd-doped GdF3 and NaGdF4 nanoparticles were synthesized and their properties were controlled by varying the stabilizer concentration and fluoride source in the reaction. These nanoparticles exhibited excellent optical and magnetic properties, and achieved superior contrast in in vivo MR imaging of pancreatic islets.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Chia-Liang Tsai, Ching-Yun Huang, Yi-Ching Lu, Li-Mei Pai, Daniel Horak, Yunn-Hwa Ma
Summary: In this study, it was found that cyclic strain reduces the internalization of magnetic nanoparticles (MNPs) by vascular smooth muscle cells (VSMCs), possibly due to decreased microvilli number and reduced vesicle size. Additionally, cyclic strain also decreases the uptake of other types of nanoparticles by cells. This finding can enhance the targeted delivery efficiency of nanomedicine.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Malgorzata Swietek, Yunn-Hwa Ma, Nian-Ping Wu, Aleksandra Paruzel, Waldemar Tokarz, Daniel Horak
Summary: In this study, tannic acid-modified silica magnetic nanoparticles were developed for antioxidant and cellular internalization purposes. The results showed that the tannic acid-modified particles exhibited high antioxidant activity and were well-internalized by LN-229 cells when a double layer of tannic acid-coating was applied. Additionally, the presence of a magnet greatly increased the cellular uptake of all tannic acid-coated magnetic nanoparticles. The coating had a significant impact on nanoparticle-cell interactions and particle internalization. These tannic acid-coated magnetic nanoparticles have great potential as intracellular carriers with preserved antioxidant activity.
Article
Chemistry, Analytical
Oleksandr Shapoval, Julian C. Brandmeier, Mykhailo Nahorniak, Viktoriia Oleksa, Ekaterina Makhneva, Hans H. Gorris, Zdenek Farka, Daniel Horak
Summary: In this study, surface engineering of upconverting nanoparticles (UCNPs) was conducted to immobilize an antibody specific to cardiac troponin I (cTnI) for the detection of cTnI biomarker. The developed method demonstrated improved sensitivity and stability compared to the conventional enzyme-linked immunosorbent assay.
Article
Biology
Mykhailo Nahorniak, Ognen Pop-Georgievski, Nadiia Velychkivska, Marcela Filipova, Eliska Rydvalova, Kristyna Gunar, Petr Matous, Uliana Kostiv, Daniel Horak
Summary: High-quality upconverting NaYF4:Yb3+,Er3+ nanoparticles were synthesized and modified with bisphosphonate-terminated poly(ethylene glycol) and Rose Bengal photosensitizer. The particles were thoroughly characterized and found to have the potential for photodynamic therapy of cancer due to the generation of reactive oxygen species under 980 nm excitation.
Article
Biochemistry & Molecular Biology
Mykhailo Nahorniak, Vitalii Patsula, Dana Marekova, Petr Matous, Oleksandr Shapoval, Viktoriia Oleksa, Magda Vosmanska, Lucia Machova Urdzikova, Pavla Jendelova, Vit Herynek, Daniel Horak
Summary: In this study, UCNPs with different surface coatings were prepared and characterized. The dissolution behavior of the particles in different media was evaluated, and the cytotoxicity and uptake by cells were assessed. The results showed that PMVEMA coating provided better protection against particle dissolution in water. However, the cytotoxicity of the particles depended not only on their ability to degrade, but also on the type of coating. UCNP@Ale-PDMA particles showed better biocompatibility compared to other surface coatings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Malgorzata Swietek, Antonin Broz, Anna Kolodziej, Jiri Hodan, Waldemar Tokarz, Anna Hlukhaniuk, Aleksandra Weselucha-Briczynska, Lucie Bacakova, Daniel Horak
Summary: Magnetic polymer nanocomposites that can be manipulated by an external magnetic field have great potential for bone tissue regeneration. In this study, a poly(epsilon-caprolactone) (PCL) matrix containing iron oxide nanoparticles stabilized by various saturated fatty acids was designed. The addition of stearic acid-stabilized magnetic nanoparticles increased the strength of the composite material, while the addition of caprylic acid-stabilized nanoparticles showed no toxicity.
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Mykhailo Nahorniak, Viktoriia Oleksa, Taras Vasylyshyn, Ognen Pop-Georgievski, Eliska Rydvalova, Marcela Filipova, Daniel Horak
Summary: In this study, hexagonal NaYF4:Yb,Er upconverting nanoparticles (UCNPs) of 171 nm in size with a narrow particle size distribution were successfully synthesized. To improve their colloidal stability in aqueous media and incorporate a photosensitizer for producing reactive singlet oxygen (O-1(2)) to kill tumor cells, UCNPs were conjugated with 6-bromohexanoic acid-functionalized Rose Bengal (RB) and coated with PEG-alendronate (PEG-Ale). Characterization using various techniques confirmed the successful synthesis and O-1(2) formation. Cytotoxicity tests on rat mesenchymal stem cells showed reduced concentration-dependent cytotoxicity after neutralization of the positive surface charge of UCNPs and incorporation of the RB sensitizer.
Article
Materials Science, Multidisciplinary
Zeinab Asghari Adib, Amir Reza Sharifi, Mohammad Ali Kiani, Hossein Yousefi, Daniel Horak, Uliana Kostiv, Ali Nabavi-Rad, Abbas Yadegar, Mohammad Yaghoubi-Avini, Hamed Golmohammadi
Summary: A biocompatible nanosensor has been developed for diagnosing Helicobacter pylori (H. pylori) infection, utilizing changes in pH-dependent fluorescence/color profile caused by the enzymatic reaction of H. pylori urease enzyme with urea. A portable 3D-printed smartphone-based imaging platform was fabricated for monitoring the nanosensor. This smart nanosensor shows potential for non-invasive diagnosis of H. pylori infection and offers insights for the development of innovative ingestible/swallowable sensors for non-invasive gastrointestinal diagnostics.
MATERIALS ADVANCES
(2023)
Article
Chemistry, Physical
Yiping Yin, Zhe Wang, Hua Zou
Summary: This study presents a novel method for preparing dimpled polymer-silica nanocomposite particles using interfacial swelling-based seeded polymerization. The optimized conditions allow for a relatively high percentage of dimpled particles to be achieved.
Article
Chemistry, Physical
Brenden D. Hoehn, Elizabeth A. Kellstedt, Marc A. Hillmyer
Summary: Porous materials with nanometer-scale pores have important applications as nanoporous membranes. In this study, ABA triblock copolymers were used as precursors to produce nanoporous polymeric membranes (NPMs) in thin film form by degrading the end blocks. Polycyclooctene (PCOE) NPMs with tunable pore sizes were successfully prepared using solvent casting technique. Oxygen plasma etching was employed to improve the surface porosity and hydrophilicity of the membranes. This study provides a straightforward method to produce tough NPMs with high porosity and hydrophilic surface properties.
Article
Chemistry, Physical
Vladislav S. Petrovskii, Stepan I. Zholudev, Igor I. Potemkin
Summary: This article investigates the behavior of linear and ring polypeptide chains in aqueous solution and explores the properties of the complexes formed by these chains with oppositely charged surfactants. The results demonstrate that the complexes of linear supercharged unfolded polypeptides and the corresponding surfactants exhibit impressive adhesive properties.
Article
Chemistry, Physical
Merve Cevik, Serkan Dikici
Summary: Cardiovascular diseases are a leading cause of death globally, and vascular grafts are a promising treatment option. This study focuses on tissue-engineered vascular grafts (TEVGs) using decellularized parsley stems as a potential biomaterial. The decellularized parsley stems showed suitable properties for TEVGs, providing a suitable environment for human endothelial cells to form a pseudo endothelium. This study showcases the potential of using parsley stems for TEVGs.
Article
Chemistry, Physical
Gustavo A. Vasquez-Montoya, Tadej Emersic, Noe Atzin, Antonio Tavera-Vazquez, Ali Mozaffari, Rui Zhang, Orlando Guzman, Alexey Snezhko, Paul F. Nealey, Juan J. de Pablo
Summary: The optical properties of liquid crystals are typically controlled by electric fields. In this study, we investigate the effects of microfluidic flows and acoustic fields on the molecular orientation and optical response of nematic liquid crystals. We identify several previously unknown structures and explain them through calculations and simulations. These findings hold promise for the development of new systems combining sound, flow, and confinement.
Article
Chemistry, Physical
Xinjun Wu, Xin Guan, Shushu Chen, Jiangpeng Jia, Chongyi Chen, Jiawei Zhang, Chuanzhuang Zhao
Summary: This research presents a novel shape memory hydrogel with a remodelable permanent shape and programmable cold-induced shape recovery behavior. The hydrogel is prepared using specific treatment methods to achieve shape fixation by heating and shape recovery by cooling. Additionally, deformable devices can be obtained by assembling hydrogel blocks with different concentrations.
Article
Chemistry, Physical
Rebecca Hengsbach, Gerhard Fink, Ulrich Simon
Summary: This study examines the properties of DNA functionalized pNipmam microgels and pure pNipmam microgels at different concentrations of sodium chloride and in PBS solutions using temperature dependent H-1-NMR measurements. The results show that DNA modification affects the volume phase transition temperature and the addition of salt and PBS further enhances this effect.
Article
Chemistry, Physical
Ningyi Li, Junhong Li, Lijingting Qing, Shicheng Ma, Yao Li, Baohui Li
Summary: This paper investigates the self-assembly behavior of colloids with competing interactions under spherical confinement and finds that different ordered structures can be formed under different sized spherical confinements. Moreover, more perforated structures are formed in smaller spheres.