Article
Polymer Science
Himani Bisht, Jaehoon Jeong, Yubin Hong, Suho Park, Daewha Hong
Summary: A tyrosine-based azido derivative (TBAD) that can perform substrate-independent surface coating and clickable film functionalization is synthesized. The TBAD remains stable under alkaline and neutral conditions and is oxidized by tyrosinase to initiate melanin-like polymerization. The resulting poly(TBAD) film can be formed on various substrates and undergo click reaction with terminal alkyne moieties. This clickable coating technique could be significant for bioconjugation, soft lithography, and labeling techniques.
MACROMOLECULAR RAPID COMMUNICATIONS
(2022)
Article
Polymer Science
Pu Li, Qiang Liu, Zhenni Xiang, Jian Wang, Wan-Xia Wu, Wen-Jing Yi
Summary: Reactive oxygen species-responsive amphiphilic polymer was synthesized for the first time using enzyme-catalyzed polymerization. Crosslinked micelles showed better stability and ROS-responsiveness compared to uncrosslinked micelles.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Parveen Kumar, Seon-Hwa Kim, Sonyabapu Yadav, Sung-Han Jo, Seong Yoo II, Sang-Hyug Park, Kwon Taek Lim
Summary: Cross-linking of nanocarriers allows targeted drug release, addressing issues such as drug leakage and side effects, thereby improving the efficacy of nanomedicines.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Polymer Science
Yile Niu, Yanbing Lu
Summary: Polycarbonate-based core crosslinked micelles (CCLMs) were constructed using photo-induced thiol-yne click reaction to improve stability and prevent premature leakage. The CCLMs exhibited pH-responsiveness and showed excellent biocompatibility and low toxicity in biological tests. DOX-encapsulated CCLMs demonstrated good anticancer effects and high internalization efficiency.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Xiao-Guang Yang, Feng-Huan Du, Jun-Jie Li, Chi Zhang
Summary: A practical method for dehydroxyazidation of alcohols using PPh3 and hypervalent azido-iodine(III) reagents is reported. The method allows for the late-stage azidation of structurally complex alcohols and the synthesis of the antiepileptic drug rufinamide.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Physical
Zhiyuan Wei, Zihao Guan, Naying Shan, Hui Li, Yan Fang, Yang Zhao, Lulu Fu, Zhipeng Huang, Mark G. Humphrey, Chi Zhang
Summary: A porphyrin covalently functionalized MoS2 nanohybrid with excellent optical properties, including reverse saturable absorption and strong self-focusing, is synthesized. This material shows great potential in optical applications, providing new possibilities for tuning nonlinear optical properties and expanding application scope.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Chemistry, Multidisciplinary
Vladimir Kardelis, Maria M. Denk, Alex Adronov
Summary: A soluble poly(tetrazine) polymer was prepared through Suzuki polycondensation, which can efficiently react with TCO derivatives via Diels-Alder click reactions. After oxidation, the resulting polymers showed enhanced fluorescence intensity and longer retention times, while maintaining relatively constant degrees of polymerization. Graft copolymers and cross-linked foam were easily prepared by reacting with TCO-functionalized poly(ethylene glycol) chains and a bis-TCO crosslinker, respectively.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Environmental
Roham Ghanbari, Ali Akbar Heidari, Hossein Mahdavi
Summary: In this study, nano-starch particles were synthesized using a Click reaction-based strategy. The use of dopamine, thioglycolic acid, and polyethyleneimine (PEI) effectively addressed the issues of poor miscibility and bacterial contamination associated with starch. The core-shell antibacterial nanostarch particles were then incorporated into a polyvinylidene fluoride (PVDF) membrane to enhance its hydrophilicity and separation performance. The optimized membrane exhibited high dye rejection rates and improved water permeation, antifouling performance, and long-term stability. Furthermore, the antibacterial evaluation demonstrated that PEI significantly enhanced the membrane's antibacterial performance.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Rong Xu, Shixin Cheng, Xu Cheng, Lv Qi, Jing Zhong, Gongping Liu, Meina Huang, Priyanka Wasnik, Qinglong Jiang
Summary: A new strategy is proposed to enhance the performance of hybrid organosilica membranes by in situ carboxyl functionalization. The introduced carboxyl functionalities result in a compact pore structure and improved water affinity, leading to an increased membrane permeability without compromising water/salt selectivity in reverse osmosis. The carboxyl functionalized organosilica membrane also demonstrates high durability during continuous reverse osmosis desalination, showing great potential for environmental and industrial applications as an efficient and robust molecular separation membrane.
ADVANCED COMPOSITES AND HYBRID MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Hao Yu, Ziteng Guo, Ningxu Han, Junjuan Shi, Xin Jiang, Qixia Bai, Zhe Zhang, Pingshan Wang, Ming Wang
Summary: This study proposes a strategy for synthesizing molecular cages by endo-modification of steric hindrance groups on dissymmetrical ligands. The resulting cages have low symmetry and dissimilar chemical environments, which has significant implications for constructing new supramolecular systems.
CELL REPORTS PHYSICAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Kai Klein, Kateryna Loza, Marc Heggen, Matthias Epple
Summary: In this study, glutathione-functionalized gold nanoparticles were azidated and covalently functionalized through a click reaction successfully coupling alkyne-functionalized dyes. The surface composition and successful azidation were confirmed by NMR and UV/vis spectroscopy. The dye-clicked nanoparticles exhibited high fluorescence, high yield, and applicability for metallic nanoparticles.
Article
Polymer Science
Seonghyeon Ahn, Hyeongkeon Yoon, Chao Duan, Weihua Li, Jin Kon Kim
Summary: This study prepared core-satellite micelles using a linear tetrablock copolymer and observed different shapes of micelles. A core-satellite structure was observed when the volume fraction was large, while a separate satellite structure was observed when the volume fraction was small.
Article
Chemistry, Physical
Jia Tian, Song-Hai Xie, Ufuk Borucu, Shixing Lei, Yifan Zhang, Ian Manners
Summary: High-resolution cryo-electron microscopy is used to obtain detailed structures of solvated corona chains and sub-nanometre crystalline core lattice of polymer-based nanofibres in solution. This research provides important insights into the formation of uniform core-shell micellar nanoparticles using crystallizable block copolymers and pi-stacking molecular amphiphiles. The findings show the highly ordered crystal lattice of poly(ferrocenyldimethylsilane)-b-4-vinylpyridine nanofibres, providing a basis for further molecular modelling analysis.
Article
Polymer Science
Wansheng Zong, Weidong Qiu, Peng Yuan, Fanfan Wang, Yingliang Liu, Shengan Xu, Shi-Jian Su, Shaokui Cao
Summary: A post-functionalization strategy was used to synthesize self-host TADF conjugated polymers, which successfully addressed the issues of complicated synthetic process and low synthetic repeatability for TADF polymers with multiple functional components.
Article
Chemistry, Multidisciplinary
Qianqian Qi, Yutong Zhang, Wei Xiong, Xingyu Liu, Shuangyu Cui, Xiaofang Ye, Kaisong Zhang, Tian Tian, Xiang Zhou
Summary: In this study, the norbornene-tetrazine ligation chemistry is used to develop clickable RNA switches in biological contexts, and its potential applications are explored. The application of RNA-based norbornene-tetrazine ligation chemistry in controlling CRISPR systems and manipulating gene editing in human cells is successfully demonstrated.
Article
Chemistry, Multidisciplinary
Fei Liu, Dmytro Danylchuk, Bohdan Andreiuk, Andrey S. Klymchenko
Summary: Organelle-specific targeting is crucial for enhancing the effectiveness of drugs and visualizing cellular processes. In this study, a novel concept of dynamic covalent targeting of organelles was proposed, where the molecule is conjugated with its ligand directly inside live cells through a dynamic covalent bond. Experimental results showed that this targeting method enabled fluorescence imaging in live cells and achieved preferential localization of drugs and dyes in specific organelles.
Review
Chemistry, Multidisciplinary
Andrey S. Klymchenko
Summary: Biomembranes, as lipid structures, play a crucial role in cellular processes. The development of chemical tools, such as fluorescent probes, for sensing and imaging biomembranes is a rapidly growing field. This Account focuses on advances in smart molecules for membrane targeting and sensing, with a particular emphasis on plasma membranes and organelles.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Lazare Saladin, Ophelie Dal Pra, Andrey S. S. Klymchenko, Pascal Didier, Mayeul Collot
Summary: Dual-emissive photoconvertible fluorophores (DPCFs) are useful for tracking labeled cells in bioimaging. The directed photooxidation-induced conversion (DPIC) mechanism allows efficient DPCFs to be obtained by conjugating a coumarin to aromatic singlet-oxygen reactive moieties (ASORMs). Pyrrole is a suitable ASORM that provides a high hypsochromic shift and fast conversion. By modifying pyrrole, we can tune the photoconversion properties of the DPCFs, leading to an improved dual emissive converter, SCP-Boc, with high brightness and a 63% enhanced photoconversion yield. SCP-Boc was successfully used for sequential cell photoconversion using laser scanning confocal microscopy.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Germain A. Brou, Gildas K. Gbassi, Ievgen Shulov, Aidar Seralin, Andrey S. Klymchenko, Thierry F. Vandamme, Nicolas Anton
Summary: In this study, we investigated the characterization and tracking of chemical reactions of amphiphile compounds on model interfaces. By using a custom-made surfactant and interfacial crosslinking through click chemistry, we observed changes in monolayer properties and dilatational elasticity. Through bulk phase exchange and washing, we specifically measured the interfacial effects of the entities adsorbed and trapped at the interface.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Nanoscience & Nanotechnology
Fei Liu, Nicolas Anton, Yosuke Niko, Andrey S. Klymchenko
Summary: Current biomedical applications of nanocarriers focus on controlled drug delivery, but this study proposes a different approach where toxic molecules are extracted from biological tissues using lipid nanoemulsion (NE) droplets. By designing a highly lipophilic amine (LipoAmine), cargo-aldehydes can be reacted and encapsulated inside lipid NEs through dynamic covalent chemistry. The formation of imine conjugates was validated and the NEs successfully extracted fluorescent dye and toxic aldehydes from cells, demonstrating the potential for developing detoxification platforms.
ACS APPLIED BIO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Deep Sekhar Biswas, Paraskevi Gaki, Elisabete Cruz Da Silva, Antoine Combes, Andreas Reisch, Pascal Didier, Andrey S. Klymchenko
Summary: By studying the FRET phenomenon between fluorescent organic nanoparticles, it is found that the efficiency does not follow the Forster law and can achieve high efficiency at long distances. This opens up the possibility of using advanced optical nanomaterials for amplified FRET-based biosensing.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Robin Dufossez, Sylvain Ursuegui, Stephanie Baudrey, Ketty Pernod, Safae Mouftakhir, Mustapha Oulad-Abdelghani, Michel Mosser, Guilhem Chaubet, Michael Ryckelynck, Alain Wagner
Summary: Enzyme-linked immunosorbent assay (ELISA) is a widely used method for protein detection in biological science. In this study, a novel droplet-based immunoassay format called D-SIRe was developed, which utilizes the oil/water interface as the capture and detection surface for proteins. This method is fast and sensitive, allowing the detection of analytes at the single bacteria level.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Reimi Pelletier, Dmytro I. Danylchuk, Hela Benaissa, Fanny Broch, Romain Vauchelles, Arnaud Gautier, Andrey S. Klymchenko
Summary: Genetically encoded protein labels combined with polarity-sensitive fluorescent probes provide information about the nanoscale environments of proteins within subcellular compartments. A solvatochromic HaloTag probe, NR12-Halo, specifically labels a variety of proteins localized in different cell compartments and distinguishes proteins within lipid membranes. This probe also reveals changes in protein environments during their life cycle, heterogeneity in membrane protein polarity, and the effects of mechanical stress and fatty acid diet on protein nanoenvironments. The solvatochromic HaloTag probe is a promising tool for studying protein interactions and nanoscale environments within subcellular structures.
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Chemistry, Multidisciplinary
L. Saladin, O. Dal Pra, A. S. S. Klymchenko, P. Didier, M. Collot
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Tesla Yudhistira, Elisabete Cruz Da Silva, Antoine Combes, Maxime Lehmann, Andreas Reisch, Andrey S. S. Klymchenko
Summary: This study reports a method of antibody labeling using biotinylated zwitterionic dye-loaded polymeric nanoparticles. The prepared biotinylated nanoparticles are small (14 nm) and bright, capable of carrying a large amount of cationic rhodamine dye. The method significantly improves fluorescence imaging of target epidermal growth factor receptors and becomes a useful tool in the high-sensitivity detection of disease biomarkers.
Article
Nanoscience & Nanotechnology
Robin Dufossez, Marie-Pierre Krafft, Sylvain Ursuegui, Michel Mosser, Safae Mouftakhir, Ketty Pernod, Guilhem Chaubet, Michael Ryckelynck, Alain Wagner
Summary: Droplet-based microfluidics is advancing the development of miniaturized and sensitive enzyme-linked immunosorbent assays (ELISAs). This article investigates the impact of surfactant structure on the efficiency and stability of the antibody grafting process on droplets. It demonstrates that short linkers lead to high functionalization efficiency, while long linkers enhance stability, with an intermediate size required to balance both parameters. The optimal structure is a PEG4 linker connecting a polar di-azide head and a per-fluoropolyether tail.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Multidisciplinary
Deep Sekhar Biswas, Nina Melnychuk, Caterina Severi, Pascal Didier, Andrey S. Klymchenko
Summary: Light-harvesting is a fundamental process in nature that drives the development of artificial systems for various applications. Researchers have discovered that using blank hydrophobic salts can suppress dye aggregation within nanoparticles, improving their fluorescence properties and enabling efficient energy transfer and antenna effects.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Lucie Haye, Nour Fayad, Richard C. Knighton, Antoine Combes, Olivier Jeannin, Andrey Klymchenko, Thibault Gallavardin, Niko Hildebrandt, Loic J. Charbonniere, Andreas Reisch
Summary: This study investigates the physicochemical and photophysical properties of Re diimine complexes and Cy5 dyes coencapsulated in polymer nanoparticles (NPs), and their compatibility for bioimaging. The results show that encapsulation significantly enhances the photoluminescence brightness and lifetimes of Re complexes, while coencapsulation of Cy5 leads to bright near-infrared emission upon Re complex excitation. Furthermore, the Re-Cy5 NPs exhibit high-contrast photoluminescence imaging in mammalian cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Chemistry, Multidisciplinary
Anila Hoskere Ashoka, Ilya O. Aparin, Andreas Reisch, Andrey S. Klymchenko
Summary: Brightness is crucial for (bio)molecular detection and optical bioimaging. This tutorial review provides definitions of brightness and describes the major approaches to its analysis. It also discusses the current chemical methods to combat Aggregation-Caused Quenching and compares the brightness and other properties of different fluorescent organic nanoparticles. The importance of brightness in biological applications is also analyzed.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Analytical
Robin Dufossez, Sylvain Ursuegui, Stephanie Baudrey, Ketty Pernod, Safae Mouftakhir, Mustapha Oulad-Abdelghani, Michel Mosser, Guilhem Chaubet, Michael Ryckelynck, Alain Wagner
Summary: Enzyme-linked immunosorbent assay (ELISA) is an important method for detecting proteins in biological science. By utilizing droplet-based microfluidics, miniaturized, less-consuming, and more sensitive ELISA assays can be developed by encapsulating biological samples and antibody-functionalized particles. This study introduces a new droplet surface immunoassay format that uses the oil/water phase interface as a protein capture and detection surface, eliminating the need for particles. The resulting method, called droplet surface immunoassay by fluorescence relocation (D-SIRe), demonstrates high sensitivity and rapid detection capabilities.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Yiping Yin, Zhe Wang, Hua Zou
Summary: This study presents a novel method for preparing dimpled polymer-silica nanocomposite particles using interfacial swelling-based seeded polymerization. The optimized conditions allow for a relatively high percentage of dimpled particles to be achieved.
Article
Chemistry, Physical
Brenden D. Hoehn, Elizabeth A. Kellstedt, Marc A. Hillmyer
Summary: Porous materials with nanometer-scale pores have important applications as nanoporous membranes. In this study, ABA triblock copolymers were used as precursors to produce nanoporous polymeric membranes (NPMs) in thin film form by degrading the end blocks. Polycyclooctene (PCOE) NPMs with tunable pore sizes were successfully prepared using solvent casting technique. Oxygen plasma etching was employed to improve the surface porosity and hydrophilicity of the membranes. This study provides a straightforward method to produce tough NPMs with high porosity and hydrophilic surface properties.
Article
Chemistry, Physical
Vladislav S. Petrovskii, Stepan I. Zholudev, Igor I. Potemkin
Summary: This article investigates the behavior of linear and ring polypeptide chains in aqueous solution and explores the properties of the complexes formed by these chains with oppositely charged surfactants. The results demonstrate that the complexes of linear supercharged unfolded polypeptides and the corresponding surfactants exhibit impressive adhesive properties.
Article
Chemistry, Physical
Merve Cevik, Serkan Dikici
Summary: Cardiovascular diseases are a leading cause of death globally, and vascular grafts are a promising treatment option. This study focuses on tissue-engineered vascular grafts (TEVGs) using decellularized parsley stems as a potential biomaterial. The decellularized parsley stems showed suitable properties for TEVGs, providing a suitable environment for human endothelial cells to form a pseudo endothelium. This study showcases the potential of using parsley stems for TEVGs.
Article
Chemistry, Physical
Gustavo A. Vasquez-Montoya, Tadej Emersic, Noe Atzin, Antonio Tavera-Vazquez, Ali Mozaffari, Rui Zhang, Orlando Guzman, Alexey Snezhko, Paul F. Nealey, Juan J. de Pablo
Summary: The optical properties of liquid crystals are typically controlled by electric fields. In this study, we investigate the effects of microfluidic flows and acoustic fields on the molecular orientation and optical response of nematic liquid crystals. We identify several previously unknown structures and explain them through calculations and simulations. These findings hold promise for the development of new systems combining sound, flow, and confinement.
Article
Chemistry, Physical
Xinjun Wu, Xin Guan, Shushu Chen, Jiangpeng Jia, Chongyi Chen, Jiawei Zhang, Chuanzhuang Zhao
Summary: This research presents a novel shape memory hydrogel with a remodelable permanent shape and programmable cold-induced shape recovery behavior. The hydrogel is prepared using specific treatment methods to achieve shape fixation by heating and shape recovery by cooling. Additionally, deformable devices can be obtained by assembling hydrogel blocks with different concentrations.
Article
Chemistry, Physical
Rebecca Hengsbach, Gerhard Fink, Ulrich Simon
Summary: This study examines the properties of DNA functionalized pNipmam microgels and pure pNipmam microgels at different concentrations of sodium chloride and in PBS solutions using temperature dependent H-1-NMR measurements. The results show that DNA modification affects the volume phase transition temperature and the addition of salt and PBS further enhances this effect.
Article
Chemistry, Physical
Ningyi Li, Junhong Li, Lijingting Qing, Shicheng Ma, Yao Li, Baohui Li
Summary: This paper investigates the self-assembly behavior of colloids with competing interactions under spherical confinement and finds that different ordered structures can be formed under different sized spherical confinements. Moreover, more perforated structures are formed in smaller spheres.
