Article
Physics, Multidisciplinary
N. Maridi, M. Benhamou, D. Khalil
Summary: This work investigates the structure and thermodynamic properties of a bilayer membrane composed of peptide molecules forming nanopores. The study reveals the existence of both repulsive and attractive forces between the nanopores. It is found that there is a critical nanopore density below which the nanopores do not coagulate.
CHINESE JOURNAL OF PHYSICS
(2023)
Article
Materials Science, Multidisciplinary
Tun Naw Sut, Bo Kyeong Yoon, Won-Yong Jeon, Joshua A. Jackman, Nam-Joon Cho
Summary: Supported lipid bilayer (SLB) coatings, versatile cell-membrane-mimicking biointerfaces, have been expanded for medical and biotechnology applications, facilitating various studies and applications like biosensors, diagnostics, and antifouling surfaces.
APPLIED MATERIALS TODAY
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra Stefanowska, Piotr Koprowski, Piotr Bednarczyk, Adam Szewczyk, Pawel Krysinski
Summary: In this study, the ROMK2 protein was synthesized and incorporated into lipid bilayers to investigate the effects of voltage changes and molecular modulators on channel activity.
BIOELECTROCHEMISTRY
(2023)
Article
Engineering, Chemical
Sandra L. Aristizabal, Lakshmeesha Upadhyaya, Gheorghe Falca, Abaynesh Yihdego Gebreyohannes, Mohammed Omer Aijaz, Mohammad Rezaul Karim, Suzana P. Nunes
Summary: Polyaryletherketones (PAEKs) are ultrahigh performance polymers with exceptional temperature and chemical resistance. The processability of these polymers is a major concern, but this study presents a modification-regeneration strategy to prepare porous Kepstan (R) and PEEK membranes, offering a new approach for the application of PAEKs.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Multidisciplinary Sciences
Tomas Sabirovas, Ausra Valiuniene, Gintaras Valincius
Summary: In this study, biomimetic electrodes suitable for probing protein/phospholipid interactions were created on metallurgically polished aluminum surfaces. The fusion of multilamellar vesicles on the aluminum surface formed a hybrid bilayer lipid membrane (hBLM), with its complex capacitance being typical for intact phospholipid bilayers. The presence of cholesterol in hBLMs inhibited membrane damage caused by the peptide melittin, which showed a concentration-dependent interaction with the hBLMs.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Yusuke Shimizu, Kohei Sato, Kazushi Kinbara
Summary: Inspired by natural calcium-induced reversible assembly and disassembly of membrane proteins, a phosphorylated amphiphile (PA) containing an oligo(phenylene-ethynylene) unit as a hydrophobic unit and a phosphate ester group as a hydrophilic calcium-binding unit was developed. The study demonstrated that PA can reversibly assemble and disassemble within lipid bilayer membranes in response to the sequential addition of calcium chloride and ethylene-diaminetetraacetic acid as a synthetic molecule for the first time.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Elisa Aranda, Jose A. Teruel, Antonio Ortiz, Maria Dolores Perez-Carceles, Jose N. Rodriguez-Lopez, Francisco J. Aranda
Summary: The anticarcinogenic properties of catechins, their interaction with lipids, and their potential site of action in membranes were studied. Experimental and computational research showed that catechins can modify lipid polymorphism and membrane structure and dynamics. These findings provide insights into the molecular effects of catechins and their potential impact on membrane function.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Multidisciplinary
Shoko Fujita, Izuru Kawamura, Ryuji Kawano
Summary: Nanopore sensing is a popular technique for rapid, simple, and label-free single-molecule detection. In order to apply this technology to a wide range of targets, such as polypeptides, nanopores with suitable size and structure are required. Researchers have designed a de novo fl-barrel peptide nanopore (SVG28) that is stable and monodisperse in size. They used a cell-free synthesis system to express hydrophilic variants of SVG28, which formed monodisperse nanopores with a diameter smaller than that of SVG28. This cell-free synthesizable peptide nanopore has the potential to enable systematic customization and comprehensive sequence screening of nanopores.
Review
Physics, Applied
Maurits R. R. de Planque
Summary: The ion flow through channel proteins embedded in a lipid bilayer membrane can be recorded as an electrical current, enabling biophysical characterization and pharmacological drug screening at a single-channel level. This concise review introduces the bilayer recording methodology, with an emphasis on the requirements for full electrophysiology assays. The self-assembled lipid bilayer, formed in a similar to 100 mu m diameter aperture in between two aqueous chambers, is critical. Various approaches to increase the measurement throughput by scaling to aperture arrays are discussed in terms of current-amplifier technology, bilayer stability, ion channel incorporation, system functionality and obtained single-channel data. The various bilayer recording platforms all have advantages and limitations. Combining the strengths of the different platform architectures, for example, the use of shaped apertures, will be essential to realize and also automate parallel ion channel recordings.
JAPANESE JOURNAL OF APPLIED PHYSICS
(2022)
Review
Biochemical Research Methods
Rananjaya S. Gamage, Jordan L. Chasteen, Bradley D. Smith
Summary: This article summarizes the different ways that proteins are anchored in cell membranes and the strategies used to label membrane proteins in living cells. It also describes the methods used to produce lipidated proteins. A three-component synthetic construct composed of a lipophilic membrane anchor, hydrophilic linker, and exposed functional component is used to anchor the payload on the cell surface. Hydrocarbon chains are commonly used as the membrane anchor and amphiphilic fluorescent dyes with a zwitterionic linker have high persistence in living cell membranes. A new advance is the development of switchable membrane anchors for fundamental studies and applied biomaterials.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hongmei Xu, Hyunhyuk Tae, Nam-Joon Cho, Changjin Huang, K. Jimmy Hsia
Summary: The solvent-assisted lipid bilayer (SALB) formation method is a simple and efficient microfluidic-based strategy for fabricating supported lipid bilayers (SLBs) with diverse compositions on various solid supports. Through thermodynamic modeling, this study explored the effects of experimental parameters on SALB formation, including substrate surface properties, initial lipid concentration, and temperature. The results showed that all parameters are crucial for the successful formation of high-quality SLBs. The model also provides guidance for optimizing experimental conditions for lipid membrane-related applications.
Article
Chemistry, Physical
Ricki Chairil, Noah Malmstadt
Summary: Curvature-mediated lipid-protein interactions play a crucial role in cellular reactions and mechanisms. This study investigates the membrane curvature partitioning behavior of cube-shaped CsPbBr3 quantum dots (QDs) embedded within deformed giant unilamellar vesicles (GUVs). The results show that the distribution of CsPbBr3 QDs differs significantly from that of conventional fluorophores and quasispherical CdSe core/ZnS shell QDs, suggesting that the geometry of both QDs and lipid membranes affect the curvature preferences of QDs. These findings provide insights into the aggregation of proteins induced by curvature and the interactions between lipid membranes and intercalating particles.
Article
Engineering, Chemical
Irish Valerie Maggay, Ming-Lun Yu, Da-Ming Wang, Ching-Hsueh Chiang, Yung Chang, Antoine Venault
Summary: Using 2-pyrrolidone (2P) and water as additives, skin-free polysulfone (PSf) membranes free of macrovoids were prepared by the non-solvent induced phase separation (NIPS) process. This method improves the membrane's permeability and mechanical properties while maintaining high porosity and large pores.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Evan J. G. van Aalst, Collin J. Borcik, Benjamin Wylie
Summary: Membrane proteins and polycyclic lipids coordinate phospholipid bilayer ordering, resulting in the partitioning of liquid crystalline phase. This ordering plays important roles in signal transduction in eukaryotes and pathogenicity and antimicrobial resistance in prokaryotes. Experimental evidence indicates microdomain formation and lipid ordering in native membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Review
Chemistry, Multidisciplinary
Agnes P. Girard-Egrot, Ofelia Maniti
Summary: Membrane proteins are crucial for cellular functions and understanding their functions is important for drug target development. However, studying membrane proteins is challenging as they require their native environment. Tethered-Bilayer Lipid Membranes (tBLMs) have emerged as a promising model for reconstituting membrane proteins due to their ability to provide a suitable environment and allow investigation through various analytical techniques.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Physical
Yiping Yin, Zhe Wang, Hua Zou
Summary: This study presents a novel method for preparing dimpled polymer-silica nanocomposite particles using interfacial swelling-based seeded polymerization. The optimized conditions allow for a relatively high percentage of dimpled particles to be achieved.
Article
Chemistry, Physical
Brenden D. Hoehn, Elizabeth A. Kellstedt, Marc A. Hillmyer
Summary: Porous materials with nanometer-scale pores have important applications as nanoporous membranes. In this study, ABA triblock copolymers were used as precursors to produce nanoporous polymeric membranes (NPMs) in thin film form by degrading the end blocks. Polycyclooctene (PCOE) NPMs with tunable pore sizes were successfully prepared using solvent casting technique. Oxygen plasma etching was employed to improve the surface porosity and hydrophilicity of the membranes. This study provides a straightforward method to produce tough NPMs with high porosity and hydrophilic surface properties.
Article
Chemistry, Physical
Vladislav S. Petrovskii, Stepan I. Zholudev, Igor I. Potemkin
Summary: This article investigates the behavior of linear and ring polypeptide chains in aqueous solution and explores the properties of the complexes formed by these chains with oppositely charged surfactants. The results demonstrate that the complexes of linear supercharged unfolded polypeptides and the corresponding surfactants exhibit impressive adhesive properties.
Article
Chemistry, Physical
Merve Cevik, Serkan Dikici
Summary: Cardiovascular diseases are a leading cause of death globally, and vascular grafts are a promising treatment option. This study focuses on tissue-engineered vascular grafts (TEVGs) using decellularized parsley stems as a potential biomaterial. The decellularized parsley stems showed suitable properties for TEVGs, providing a suitable environment for human endothelial cells to form a pseudo endothelium. This study showcases the potential of using parsley stems for TEVGs.
Article
Chemistry, Physical
Gustavo A. Vasquez-Montoya, Tadej Emersic, Noe Atzin, Antonio Tavera-Vazquez, Ali Mozaffari, Rui Zhang, Orlando Guzman, Alexey Snezhko, Paul F. Nealey, Juan J. de Pablo
Summary: The optical properties of liquid crystals are typically controlled by electric fields. In this study, we investigate the effects of microfluidic flows and acoustic fields on the molecular orientation and optical response of nematic liquid crystals. We identify several previously unknown structures and explain them through calculations and simulations. These findings hold promise for the development of new systems combining sound, flow, and confinement.
Article
Chemistry, Physical
Xinjun Wu, Xin Guan, Shushu Chen, Jiangpeng Jia, Chongyi Chen, Jiawei Zhang, Chuanzhuang Zhao
Summary: This research presents a novel shape memory hydrogel with a remodelable permanent shape and programmable cold-induced shape recovery behavior. The hydrogel is prepared using specific treatment methods to achieve shape fixation by heating and shape recovery by cooling. Additionally, deformable devices can be obtained by assembling hydrogel blocks with different concentrations.
Article
Chemistry, Physical
Rebecca Hengsbach, Gerhard Fink, Ulrich Simon
Summary: This study examines the properties of DNA functionalized pNipmam microgels and pure pNipmam microgels at different concentrations of sodium chloride and in PBS solutions using temperature dependent H-1-NMR measurements. The results show that DNA modification affects the volume phase transition temperature and the addition of salt and PBS further enhances this effect.
Article
Chemistry, Physical
Ningyi Li, Junhong Li, Lijingting Qing, Shicheng Ma, Yao Li, Baohui Li
Summary: This paper investigates the self-assembly behavior of colloids with competing interactions under spherical confinement and finds that different ordered structures can be formed under different sized spherical confinements. Moreover, more perforated structures are formed in smaller spheres.