Review
Chemistry, Analytical
Maedeh Khodamoradi, Saeed Rafizadeh Tafti, Seyed Ali Mousavi Shaegh, Behrouz Aflatoonian, Mostafa Azimzadeh, Patricia Khashayar
Summary: Microfluidics provides a platform for precise manipulation and separation of high-quality sperm cells through various stimuli. It can assist in guiding sperm and oocytes for controlled in vitro fertilization or sperm sorting. Different microfluidic methods have their own advantages and disadvantages, offering promising future perspectives in the field.
Article
Andrology
Kazuko Ogata, Maria Portia B. Nagata, Hirofumi Nishizono, Tadayuki Yamanouchi, Hideo Matsuda, Yuki Ogata, Kumiko Takeda, Yutaka Hashiyada, Kenichi Yamashita
Summary: This study focused on using a microfluidic sperm sorter to collect highly motile spermatozoa from frozen-thawed bull semen and found that the sorted spermatozoa exhibited significantly higher motility, membrane integrity, mitochondrial function, and ATP production compared to unsorted spermatozoa. The results suggest that the device has great potential in assisted reproduction by strictly selecting high-quality spermatozoa.
Article
Obstetrics & Gynecology
Molly M. Quinn, Salustiano Ribeiro, Flor Juarez-Hernandez, Rhodel K. Simbulan, Liza Jalalian, Marcelle Cedars, Mitchell P. Rosen
Summary: There was no significant difference in embryo quality, clinical pregnancy rates, and ongoing pregnancy rates between using a microfluidic sperm separation device and density-gradient centrifugation in IVF with ICSI.
HUMAN REPRODUCTION
(2022)
Article
Chemistry, Analytical
Shih-Jui Huang, Chia-Ming Chang, Yen-Ta Lu, Cheng-Hsien Liu
Summary: This study developed an integrated microfluidic biochip to separate target tumor cells and clusters from whole blood using a two-stage sorting mechanism. The chip achieved high recovery rates and purity in separating tumor cell clusters, making it suitable for cancer research and clinical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Sandhya Sharma, Md Alamgir Kabir, Waseem Asghar
Summary: This study develops a microfluidic chip that isolates healthy sperm cells by taking advantage of their natural rheotaxis behavior. The chip consists of chambers and microchannels that separate motile sperm cells from unprocessed semen samples using fluid flow. The results show high motility and low sperm DNA fragmentation.
Article
Obstetrics & Gynecology
Muge Keskin, Emre Goksan Pabuccu, Tufan Arslanca, Ozgur Dogus Demirkiran, Recai Pabuccu
Summary: This study aimed to compare the effects of microfluidic sperm sorting and conventional density gradient centrifugation on embryo quality and euploidy rates. The study found that the MSS group had significantly higher total blastocyst and top quality blastocyst numbers, but there was no improvement in euploidy rates and live birth rates.
REPRODUCTIVE SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Albert Salas-Huetos, Jordi Ribas-Maynou, Yentel Mateo-Otero, Carolina Tamargo, Marc Llavanera, Marc Yeste
Summary: This study investigates the expression and function of miRNA in bovine sperm. The results show that 8 out of 10 candidate miRNAs are consistently detected in bovine sperm, with one miRNA (bta-miR-138) being significantly lower in the subfertility group compared to the high fertility group. Additionally, three other miRNAs (bta-miR-19b, bta-miR-26a, and bta-miR-7) are correlated with sperm function.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Obstetrics & Gynecology
Elisabetta Baldi, Meurig T. Gallagher, Stepan Krasnyak, Jackson Kirkman-Brown
Summary: The sixth edition of the World Health Organization manual explores extended examination methods that offer key diagnostics for investigating male reproductive system function. These methods go beyond basic semen analysis and can specifically guide the clinical characterization of fertile or infertile men. Multiparametric scoring for sperm morphological defects, sperm DNA fragmentation, and computer-assisted analysis of sperm or semen are among the most widely used and debated techniques.
FERTILITY AND STERILITY
(2022)
Article
Biochemical Research Methods
Sadat Hasan, Maximilian E. Blaha, Sebastian K. Piendl, Anish Das, David Geissler, Detlev Belder
Summary: This research introduces a label-free detection technique using time-resolved two-photon excitation (TPE) fluorescence detection at 532 nm in droplet microfluidics. A polydimethylsiloxane-fused silica (FS) hybrid chip was developed to provide high optical transparency in the deep-UV range and suitable surface properties for droplet microfluidics. The system incorporating a 532-nm picosecond laser, time-correlated single-photon counting (TCSPC), and a chip-integrated dielectrophoretic pulsed actuator was utilized to sort droplets containing specific compounds and screen yeast cells based on their protein autofluorescence.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Junyu Ma, Yihui Wu, Yongshun Liu, Yuan Ji, Mei Yang, Hongquan Zhu
Summary: The centrifugal microfluidic chip transforms non-steady flow into locally steady flow with a flow rectifier, which improves separation purity and recovery ratio of target particles. It can be used to separate particles of differing sizes, including rare tumor cells. Experimental results show that the chip has achieved good separation purity and recovery ratio.
Article
Cell Biology
Tomer Avidor-Reiss, Luke Achinger, Rustem Uzbekov
Summary: Centrioles are subcellular organelles that are crucial for normal cell function and development. They are found in most animal cell types but are absent in egg cells and exhibit diverse characteristics in sperm cells. The precise role of centrioles in fertility and early embryo development remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Anais Vitorino Carvalho, Laura Soler, Aurore Thelie, Isabelle Grasseau, Luiz Cordeiro, Daniel Tomas, Ana-Paula Teixeira-Gomes, Valerie Labas, Elisabeth Blesblois
Summary: Using two complementary proteomic methods, this study identified 57 differentially abundant proteins in fertile and subfertile rooster sperm. These proteins are involved in various molecular pathways such as flagellum integrity and movement, mitochondrial functions, sperm maturation, storage in female tract, and oocyte-sperm interaction. The data provides new insights into the complexity of male fertility in chickens and suggests the use of a combination of markers for predicting male fertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Byeong-Mu Lee, Yoo-Jin Park, Won-Ki Pang, Do-Yeal Ryu, Md Saidur Rahman, Dae-Young Lee, Myung-Geol Pang
Summary: The activation of mitochondria in spermatozoa during capacitation leads to the changes in biological and morphological features of spermatozoa that are necessary for fertilization. This study focused on identifying the changes in mitochondrial proteins during sperm capacitation and their regulation of male fertility using boar spermatozoa. The expression of different mitochondrial proteins varied during sperm capacitation, depending on the fertility status of the boars.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Saurabh Tiwari, Gayathri Rajamanickam, Veena Unnikrishnan, Mina Ojaghi, John P. Kastelic, Jacob C. Thundathil
Summary: An advanced understanding of sperm function is important for male fertility prediction and addressing male infertility. Current methods for evaluating fertility overlook subtle differences in sperm, and research is now focused on developing in vitro sperm function tests and identifying biomarkers. This review discusses the role of the ATP1A4 protein in capacitation and fertilization events, and its potential use in improving male fertility prediction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Aylin Nikshad, Afsoon Aghlmandi, Reza Safaralizadeh, Leili Aghebati-Maleki, Majid Ebrahimi Warkiani, Farhad Motavalli Khiavi, Mehdi Yousefi
Summary: According to the World Health Organization, approximately 70 million couples worldwide suffer from infertility. Research teams have integrated various scientific branches to develop therapeutic approaches, with Assisted Reproductive Technologies (ART) being a key solution. The use of microfluidic technology has shown significant progress in improving efficiency in assisted reproduction.
Review
Materials Science, Multidisciplinary
Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, Ali K. Yetisen, Savas Tasoglu
Summary: LAMP technology is a fast, efficient, and low-cost diagnostic method for pathogen detection, which can be applied to various bacteria, viruses, and parasitic pathogens. In addition, LAMP technology can also be used for SNP genotyping and mutation detection.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Materials Science, Biomaterials
Hossein Derakhshankhah, Morteza Eskandani, Sattar Akbari Nakhjavani, Savas Tasoglu, Somayeh Vandghanooni, Mehdi Jaymand
Summary: Researchers successfully developed an innovative electrically conductive hydrogel for bone tissue engineering by incorporating silica nanoparticles and poly(aniline-co-dopamine) into oxidized alginate. The hydrogel exhibited high electrical conductivity and swelling ratio. Cytocompatibility and cell proliferation potential of the hydrogel scaffold were confirmed, and its potential for hydroxyapatite formation and bioactivity induction was demonstrated.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Review
Chemistry, Medicinal
Hassan Nasrollahpour, Balal Khalilzadeh, Mohammad Hasanzadeh, Reza Rahbarghazi, Pedro Estrela, Abdolhossein Naseri, Savas Tasoglu, Mika Sillanpaa
Summary: Breast cancer is the most common cancer type among women globally, and timely diagnosis is crucial for reducing mortality rate and socio-economic burden. This review comprehensively assesses the use of nanomaterials and related technologies for breast cancer diagnosis. The growing use of nano/biotechnology in electrochemical nanobiosensor designing is discussed in detail, along with recent advances in nanomaterial-based biosensing methodologies and their advantages and disadvantages. The review also focuses on designing methods, advantages, and the necessity of suitable materials from a statistical standpoint. The main objective is to classify applicable biosensors based on breast cancer biomarkers.
MEDICINAL RESEARCH REVIEWS
(2023)
Article
Chemistry, Analytical
Sattar Akbari Nakhjavani, Balal Khalilzadeh, Hadi Afsharan, Nashmin Hosseini, Mohammad Hossein Ghahremani, Sandro Carrara, Savas Tasoglu, Yadollah Omidi
Summary: Recently, there has been increased interest in electrochemiluminescent (ECL) immunosensors for biomarker detection. A highly enhanced ECL immunosensing platform was designed for ultrasensitive detection of carcinoembryonic antigen (CEA) using functional nanostructures. By entrapping CEA biomolecules using a sandwich approach and incorporating luminol/silver nanoparticles as the signaling probe, the immunosensor exhibited excellent sensitivity and selectivity for CEA detection. It also demonstrated good repeatability and stability in real human serum, making it a potential candidate for clinical CEA detection and monitoring.
Article
Hematology
Dorota Szpak, Chloe Turpin, Utku Goreke, Katarzyna Bialkowska, Kamila M. Bledzka, Dmitriy Verbovetskiy, Narla Mohandas, Umut A. Gurkan, Jun Qin, Edward F. Plow, Elzbieta Pluskota
Summary: Kindlin-3 (K3) is crucial for the activation of integrin adhesion receptors in hematopoietic cells. Deficiency of K3 in erythroid and myeloid cells leads to anemia and misshaped erythrocytes, respectively. K3 has a dual function in erythrocytes and erythroblastic islands, playing a critical role in erythropoiesis and maintaining the integrity of erythrocyte shape through F-actin binding.
Review
Biochemical Research Methods
Abdollah Ahmadpour, Pelin Kubra Isgor, Berk Ural, Busra Nimet Eren, Misagh Rezapour Sarabi, Metin Muradoglu, Savas Tasoglu
Summary: This paper reviews the designs and applications of microneedle arrays, which are micro- and nano-scale patches of needles that have been combined with microfluidic systems to create more advanced devices for biomedical applications, including drug delivery, wound healing, biosensing, and body fluid sampling. The paper also discusses modeling approaches for fluid flow and mass transfer in microneedle designs and highlights the challenges.
Editorial Material
Pathology
Berin Ozdalgic, Ali K. Yetisen, Savas Tasoglu
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Article
Engineering, Biomedical
Zoe Sekyonda, Ran An, Alireza Avanaki, Arwa Fraiwan, Umut A. Gurkan
Summary: Effective management of diabetes relies on early diagnosis and long-term regulation of blood glucose. The levels of glycohemoglobin (HbA1 and HbA1c) are commonly used biomarkers for diabetes diagnosis and management. However, current point-of-care tests for glycohemoglobin may produce inaccurate results for patients with co-existing diseases. In this study, a paper-based affinity electrophoresis platform called HemeChip-GHb was developed for accurate HbA1 detection.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Sattar Akbari Nakhjavani, Begum K. Tokyay, Cansu Soylemez, Misagh R. Sarabi, Ali K. Yetisen, Savas Tasoglu
Summary: Prostate cancer is one of the common tumors and leading causes of male mortality. Various biosensors have been developed for the detection of prostate cancer biomarkers, but there are some limitations. New technologies are being utilized for the development of more practical biosensors.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Misagh Rezapour Sarabi, Ahmet Agah Karagoz, Ali K. Yetisen, Savas Tasoglu
Summary: The field of microrobots is rapidly advancing, aiming to develop new capabilities for biomedical applications including drug delivery, surgical procedures, tracking and imaging, and sensing. Magnetic control of microrobot motion is emerging as a promising approach for these applications. This paper introduces 3D printing methods for microrobot fabrication and discusses their future perspectives towards enabling clinical translation.
Review
Materials Science, Biomaterials
Misagh Rezapour Sarabi, Ali K. Yetisen, Savas Tasoglu
Summary: Bioprinting utilizes 3D printing techniques to print tissues and organs for biomedical engineering. Conducting bioprinting in space, where there is no gravity, can advance tissue engineering. Printing of soft tissues, which are prone to collapsing, can be accelerated in microgravity. Moreover, bioprinting in space can support human colonization by providing necessary resources through 3D printing, such as living engineered filters, eliminating dependence on Earth's supplies.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Paulami Dam, Merve Celik, Merve Ustun, Sayantan Saha, Chirantan Saha, Elif Ayse Kacar, Senanur Kugu, Elif Naz Karagulle, Savas Tasoglu, Fatih Buyukserin, Rittick Mondal, Priya Roy, Maria L. R. Macedo, Octavio L. Franco, Marlon H. Cardoso, Sevde Altuntas, Amit Kumar Mandal
Summary: Wound healing is a complex process involving various cell types and conditions. Nanoparticle-based materials have been proposed as a therapeutic approach for wound healing due to their antibacterial activity, biocompatibility, and increased mechanical strength. Different types of nanoparticles can be used depending on the type, depth, and area of the wound.
Article
Biochemical Research Methods
Michaela B. B. Cooley, William J. J. Wulftange, Dana Wegierak, Utku Goreke, Eric C. C. Abenojar, Umut A. A. Gurkan, Agata A. A. Exner
Summary: Lipid shell-stabilized nanoparticles, known as nanobubbles, have emerged as a promising contrast agent for molecular ultrasound imaging. This study presents a microfluidic chip and imaging method that allows real-time imaging and characterization of the extravasation process of nanobubbles through an extracellular matrix. The results demonstrate the faster diffusion of nanobubbles in a collagen matrix with smaller pore size, compared to larger nanobubbles. The combination of ultrasound imaging and microfluidic chip provides valuable insights into the spatiotemporal movement of nanoparticles in a heterogeneous extracellular matrix.
Review
Obstetrics & Gynecology
Mert Yasli, Sajjad Rahmani Dabbagh, Savas Tasoglu, Serdar Aydin
Summary: Three-dimensional printing, also known as additive manufacturing, allows the creation of complex structures out of a digital model. Its applications in medical field, such as orthopedics, dentistry, urology, and reconstructive surgery, have been rapidly developing. Obstetrics and Gynecology is an emerging application field for 3D printing, which can be used in preventive medicine, diagnosis, treatment, and surgical training.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
Review
Chemistry, Multidisciplinary
Sajjad Rahmani Dabbagh, Misagh Rezapour Sarabi, Mehmet Tugrul Birtek, Nur Mustafaoglu, Yu Shrike Zhang, Savas Tasoglu
Summary: Organ-on-a-chip (OOC) platforms provide a more realistic environment compared to animal models and conventional cell cultures, offering continuous perfusion of cell cultures, integration of biosensors, and 3D structures fabricated through bioprinting. These platforms have great potential for understanding organ functions, studying disease impacts, and drug screening. This review presents an overview of bioprinting techniques, their advantages and limitations, recent advancements, applications, and future prospects of the 3D-bioprinted OOC platforms, as well as current challenges and future directions.
