Article
Immunology
Cameron R. Bishop, Felipe Ten Caten, Helder I. Nakaya, Andreas Suhrbier
Summary: This study compared the gene expression profiles between a C57BL/6J CHIKV mouse model and humans infected with CHIKV. The results showed a high degree of similarity in gene expression profiles, especially in immune pathways and signatures. The C57BL/6J CHIKV mouse model is a reliable and representative system for studying CHIKV immunopathology and evaluating new treatments.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Rebecca Floyd, Adam O. Michel, Alessandra Piersigilli, Eric Aronowitz, Henning U. Voss, Rodolfo J. Ricart Arbona
Summary: An otherwise healthy two-month-old female C57BL/6J mouse presented with a left-sided head tilt, which was found to be caused by a congenital malformation of the nasal cavity and frontal aspect of the skull known as an ethmoidal meningoencephalocele through MRI and histopathologic examination. This abnormality has not been previously reported in mice, and further research is needed to understand the etiology and potential genetic factors associated with this condition.
LABORATORY ANIMALS
(2021)
Article
Cardiac & Cardiovascular Systems
Satya Murthy Tadinada, Eric T. Weatherford, Greg Collins, Gourav Bhardwaj, Jesse Cochran, William Kutschke, Kathy Zimmerman, Alyssa Bosko, Brian T. O'Neill, Robert M. Weiss, E. Dale Abel
Summary: The study found that despite inducing cardiac hypertrophy and increasing cardiac fatty acid metabolism, long-term fat feeding in mice may not be enough to activate pathological hypertrophic mechanisms that impair cardiac function or induce cardiac fibrosis. Additionally, unknown factors may contribute to the cardiac abnormalities reported by many research groups.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Neurosciences
Laurel R. Seemiller, Lisa R. Goldberg, Phillip B. Smith, Jason Dennis, Andrew D. Patterson, Thomas J. Gould
Summary: Genetic background plays a role in sensitivity to nicotine and metabolism, which can affect susceptibility to nicotine addiction. Studying differences in nicotine sensitivity and metabolism in inbred mouse strains can help identify risk factors for nicotine addiction.
Article
Pathology
Laura J. Janke, Jerold E. Rehg
Summary: Histiocytic sarcoma is a rare tumor derived from macrophages in humans, but more common in mice. Due to its diverse cellular morphologies, growth patterns, and organ distributions, it can be difficult to diagnose and differentiate from other types of neoplasia. Immunohistochemistry (IHC) is often necessary for distinguishing histiocytic sarcomas from other tumors in mice. This article provides a comprehensive overview of the cellular morphologies, growth patterns, organ distributions, and IHC labeling of 62 mouse histiocytic sarcomas, as well as differentiating features from morphologically similar tumors.
VETERINARY PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ronak Shah, Paul C. M. van den Berk, Colin E. J. Pritchard, Ji-Ying Song, Maaike Kreft, Bas Pilzecker, Heinz Jacobs
Summary: Fanconi anemia (FA) is caused by mutation in FANC genes involved in interstrand crosslink (ICL) repair. This study established a genetically and immunologically well-defined Fancg-KO mouse model using CRISPR/Cas9, excluding confounders from drug-based selection cassettes. The Fancg-KO mice exhibited infertility, hypogonadism, and hematopoietic defects, with hypersensitivity to crosslinking agents. The project provides a valuable tool for further research on FANCG and ICL repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Hans Christian Schmidt, Johanna Hagens, Pauline Schuppert, Birgit Appl, Laia Pagerols Raluy, Magdalena Trochimiuk, Clara Philippi, Zhongwen Li, Konrad Reinshagen, Christian Tomuschat
Summary: Exposure to plant toxins or enzymatic digestion of common food by microbiota may cause biliary atresia (BA). Biliatresone, an isoflavonoid, can effectively disrupt the development of extrahepatic bile duct (EHBD) in mice. It reduces Glutathione levels, downregulates SOX17, and can be counteracted with N-Acetyl-L-cysteine treatment in vitro. The toxic effect of biliatresone was confirmed in robust C57BL/6J mice, showing similarity to BALB/c mice. Affected neonates displayed symptoms of BA, and histological analysis confirmed cholestasis. Our study adds to the evidence that biliatresone is an effective agent for targeted alteration of the EHBD system.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Linda F. Boeswald, Jasmin Wenderlein, Wolfgang Siegert, Reinhard K. Straubinger, Ellen Kienzle
Summary: Data on mineral digestibility is essential for understanding mineral homeostasis and improving dietary recommendations. While there is ample information on mineral digestibility in farm animals and pets, there is a lack of data in laboratory mice under maintenance conditions, which is crucial for studying mineral homeostasis. This study analyzed data on intake, faecal excretion, and apparent digestibility of various minerals in C57BL/6J mice fed different maintenance diets.
Article
Multidisciplinary Sciences
Mattea S. Durst, Margarete Arras, Rupert Palme, Steven R. Talbot, Paulin Jirkof
Summary: In this study, the combination of local anesthesia and systemic analgesia in mice did not show significant advantages in terms of post-surgical pain and recovery compared to animals treated with either local anesthesia or Paracetamol alone.
SCIENTIFIC REPORTS
(2021)
Article
Gastroenterology & Hepatology
Nazli Khodayari, Regina Oshins, Alek M. Aranyos, Sergio Duarte, Sayedamin Mostofizadeh, Yuanqing Lu, Mark Brantly
Summary: We characterized a mouse model of human alpha-1 antitrypsin deficiency with a strong manifestation of liver disease, which can be used as an in vivo tool for testing preventive and therapeutic reagents. Our data explored the altered immunophenotype of alpha-1 antitrypsin-deficient liver macrophages and suggested a relationship between acute inflammation, immune response, and fibrosis.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Margaret A. Hull, Penny S. Reynolds, Elizabeth A. Nunamaker
Summary: The study found that using non-aversive handling methods can improve breeding performance in laboratory mice and reduce pup mortality. Mice handled with transfer tunnels had higher numbers of pups born and weaned, and a lower risk of litter loss.
Article
Pharmacology & Pharmacy
Kenneth Alper, Janelle Cange, Ria Sah, Deanna Schreiber-Gregory, Henry Sershen, K. Yaragudri Vinod
Summary: This study investigated the effects of psilocybin on voluntary ethanol consumption in adult male and female C57BL/6J mice. The results showed that psilocybin reduced ethanol consumption and preference in male mice, but had no significant effect in female mice.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Endocrinology & Metabolism
Chenxiao Zhang, Tengfei Ma, Chang Liu, Ding Ma, Jian Wang, Meng Liu, Jinjun Ran, Xueting Wang, Xiaobei Deng
Summary: PM2.5 exposure can lead to extensive metabolic disturbances, particularly in lipid and amino acid metabolism. It is associated with the enrichment of disease pathways and signaling pathways related to non-alcoholic fatty liver disease, type II diabetes mellitus, TGF-beta, AMPK, and mTOR. Accumulation of acylcarnitine in liver tissue restricts lipid consumption and contributes to hepatic steatosis. The aberrant expression of key transcription factors, such as PPARs and SREBP1, may play a regulatory role in lipid metabolic disorders.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Nutrition & Dietetics
Susan M. Smith, Eneda Pjetri, Walter B. Friday, Brandon H. Presswood, Dane K. Ricketts, Kathleen R. Walter, Sandra M. Mooney
Summary: This study assessed the effects of prenatal alcohol exposure on behavior and metabolism in a mouse model. It found that prenatal alcohol exposure can lead to behavioral and metabolic impairments, which may change with age. The findings emphasize that prenatal alcohol exposure should be considered as a whole-body disorder.
Article
Biochemistry & Molecular Biology
Min Shin, Madhavi Pandya, Kristan Espinosa, Ravindra Telang, Jordi Boix, Peter R. Thorne, Srdjan M. Vlajkovic
Summary: The study found that weekly injections of istradefylline in middle-aged C57BL/6 mice can attenuate the progression of ARHL and improve cochlear development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Xiaoying Zhang, Aaron G. Baer, Joshua M. Price, Piet C. Jones, Benjamin J. Garcia, Jonathon Romero, Ashley M. Cliff, Weidong Mi, James B. Brown, Daniel A. Jacobson, Ralph Lydic, Helen A. Baghdoyan
JOURNAL OF NEUROPHYSIOLOGY
(2020)
Article
Neurosciences
Aaron G. Baer, Allen K. Bourdon, Joshua M. Price, Shawn R. Campagna, Daniel A. Jacobson, Helen A. Baghdoyan, Ralph Lydic
JOURNAL OF NEUROPHYSIOLOGY
(2020)
Review
Neurosciences
Jan-Marino Ramirez, Nicholas J. Burgraff, Aguan D. Wei, Nathan A. Baertsch, Adrienn G. Varga, Helen A. Baghdoyan, Ralph Lydic, Kendall F. Morris, Donald C. Bolser, Erica S. Levitt
Summary: Opioid-induced respiratory depression is the primary cause of death associated with therapeutic and recreational opioid use, with the death rate from opioid abuse growing disproportionally in the United States since the early 1990s. Despite uncovering many fundamental cellular and systems-level mechanisms related to opioid-related death, factors such as individual vulnerability, neuromodulatory compensation, and redundancy of opioid effects continue to hinder the formation of a concise, integrative view of OIRD. This review brings together multiple perspectives in the field of OIRD to create a comprehensive understanding of this important research topic and its future direction.
JOURNAL OF NEUROPHYSIOLOGY
(2021)
Article
Neurosciences
Christopher B. O'Brien, Clarence E. Locklear, Zachary T. Glovak, Diana Zebadua Unzaga, Helen A. Baghdoyan, Ralph Lydic
Summary: Antinociceptive doses of fentanyl, morphine, and buprenorphine significantly and differentially disrupt EEG-defined states of consciousness in C57BL/6J mice. These findings contribute to understanding the impact of opioid drugs on sleep and consciousness states.
JOURNAL OF NEUROPHYSIOLOGY
(2021)
Review
Physiology
Ralph Lydic, Helen A. Baghdoyan
Summary: Changes in wakefulness caused by opioids, anesthetics, and sleep alter concentrations of molecules in the prefrontal cortex (PFC) metabolome. It is crucial to quantify state-selective changes in the PFC metabolome for advancing functional metabolomics. The diverse functions of the PFC indicate its metabolome as a potential therapeutic target for counteracting state-selective autonomic dysfunction.
Article
Physiology
Zachary T. Glovak, Helen A. Baghdoyan, Ralph Lydic
Summary: This study found that opioids have specific effects on breathing, and that increased cholinergic activity can alleviate the decrease in minute ventilation caused by opioids.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2022)
Article
Physiology
Zachary T. Glovak, Chelsea Angel, Christopher B. O'Brien, Helen A. Baghdoyan, Ralph Lydic
Summary: The study found that the opioid buprenorphine can cause changes in breathing, and leptin status is an important factor in modulating these changes. Obesity and leptin resistance increase the risk of respiratory disorders. The effects of buprenorphine vary significantly in mice with different leptin statuses and sexes.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2022)
Editorial Material
Clinical Neurology
Philippe Haouzi, Ralph Lydic
Article
Multidisciplinary Sciences
Haden L. Scott, Dima Bolmatov, Peter T. Podar, Zening Liu, Jacob J. Kinnun, Benjamin Doughty, Ralph Lydic, Robert L. Sacci, C. Patrick Collier, John Katsaras
Summary: This study demonstrates that droplet interface bilayers (DIBs) composed of lipids can exhibit persistent synaptic plasticity, characterized by long-term potentiation (LTP) and capacitive energy storage. The time scales for the physical changes associated with LTP are longer than previous short-term synaptic plasticity studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Psychology, Clinical
Gretchen Kurdziel-Adams, Jenny Macfie, Stephanie Kors, Kimberly B. Fortner, Craig Towers, Ralph Lydic
PERSONALITY DISORDERS-THEORY RESEARCH AND TREATMENT
(2020)