Article
Cell & Tissue Engineering
Julien Guerrero, Boris Dasen, Agne Frismantiene, Sebastien Pigeot, Tarek Ismail, Dirk J. Schaefer, Maria Philippova, Therese J. Resink, Ivan Martin, Arnaud Scherberich
Summary: The presence of T-cadherin-positive cells derived from the stromal vascular fraction (SVF) is essential for the osteogenic and vasculogenic properties of SVF cells. The crosstalk between stromal cells and T-cadherin-expressing endothelial cells within adipose tissue critically regulates osteogenesis.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Roman Vuerich, Elena Groppa, Simone Vodret, Nadja Annelies Ruth Ring, Chiara Stocco, Fleur Bossi, Chiara Agostinis, Matteo Cauteruccio, Andrea Colliva, Mohammad Ramadan, Francesca Simoncello, Federica Benvenuti, Anna Agnelli, Franca Dore, Flavia Mazzarol, Massimo Moretti, Alice Paulitti, Silvia Palmisano, Nicolo De Manzini, Mattia Chiesa, Manuel Casaburo, Angela Raucci, Daniela Lorizio, Giulio Pompilio, Roberta Bulla, Giovanni Papa, Serena Zacchigna
Summary: Nonhealing wounds have a negative impact on the quality of life for patients and health systems. Skin substitutes have been used, but have limited efficacy due to inadequate vascularization. The use of the stromal vascular fraction (SVF) from adipose tissue shows promise in overcoming this limitation, but additional research is needed to determine its precise mechanism of action.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Yoshitaka Iso, Sayaka Usui, Hiroshi Suzuki
Summary: The aim of this study was to investigate the pro-angiogenic potential of skeletal muscle mesenchymal stem/stromal cells (mMSCs). The results showed that PDGFR-alpha positive mMSCs secreted VEGF and hepatocyte growth factor, inducing endothelial tube formation and promoting capillary growth. Epo stimulation enhanced phosphorylation and cellular proliferation in mMSCs, leading to improved perfusion recovery and capillary growth in the ischemic limbs.
Article
Cell & Tissue Engineering
Tomoyuki Kamenaga, Yuichi Kuroda, Kanto Nagai, Masanori Tsubosaka, Yoshinori Takashima, Kenichi Kikuchi, Masahiro Fujita, Kemmei Ikuta, Kensuke Anjiki, Toshihisa Maeda, Naoki Nakano, Koji Takayama, Shingo Hashimoto, Shinya Hayashi, Takehiko Matsushita, Takahiro Niikura, Ryosuke Kuroda, Tomoyuki Matsumoto
Summary: The study found that SVF cells can enhance bone healing, with cryopreserved cells showing almost equal potential as fresh cells. SVF cells can be used to improve nonunion bone fracture healing as an alternative to other mesenchymal stem cells, and their effect can be maintained under cryopreservation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Tim Tian Y. Han, John T. Walker, Aaron Grant, Gregory A. Dekaban, Lauren E. Flynn
Summary: The study shows that bioreactor preconditioning enhances the regenerative potential of human ASCs by altering the expression characteristics of ASCs on DAT scaffolds through in vitro culture, and also promotes the infiltration of host cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hanis Nazihah Hasmad, Ruszymah Bt Hj Idrus, Nadiah Sulaiman, Yogeswaran Lokanathan
Summary: The study evaluated the effects of different fiber thicknesses of EF-HAM scaffolds on the paracrine behavior of SkM. The results showed that all thicknesses of EF-HAM demonstrated excellent biocompatibility with SkM. CM obtained from EF-HAM 7 min scaffolds contained significantly higher levels of proangiogenic factors compared to plain CM, and enhanced the viability and migration capacity of HUVECs. These findings suggest that EF-HAM scaffolds have angiogenic properties and can be used for the repair and regeneration of ischemic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Jiashing Yu, Yi-Chiung Hsu, Jen-Kuang Lee, Nai-Chen Cheng
Summary: In this study, the integration of adipose-derived stem cell (ASC) spheroids into ASC sheets was investigated to enhance the angiogenic capability of cell sheets. The results showed that ASC spheroids have the potential to promote endothelial cell differentiation and angiogenesis, making them a promising three-dimensional culture technique for future application in angiogenesis and regeneration therapies.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Elle Koivunotko, Jasmi Snirvi, Arto Merivaara, Riina Harjumaki, Swarna Rautiainen, Minna Kelloniemi, Kirsi Kuismanen, Susanna Miettinen, Marjo Yliperttula, Raili Koivuniemi
Summary: Adipose-derived mesenchymal stromal cells (ASCs) hold great potential for cellular therapies and can differentiate into endothelial cells (ECs) and other angiogenic cell types. Nanofibrillated cellulose (NFC) hydrogel is a suitable matrix for 3D cell culture of ASCs and can stimulate angiogenesis. This study recommends the use of NFC hydrogel as an animal-free biomaterial scaffold for therapeutic angiogenesis and other cell therapy purposes.
Article
Cardiac & Cardiovascular Systems
Yiwen Xu, Aaron D. Ward, Daniel Goldman, Hao Yin, John-Michael Arpino, Zengxuan Nong, Jason J. Lee, Caroline O'Neil, J. Geoffrey Pickering
Summary: Machine learning and micromorphometry were used to analyze arteriolar landscape in regenerating muscles after ischemic injury in mice. The study found an increase in arteriolar density, decreased lumen caliber, and reduced smooth muscle content in the regenerated muscles. Computational modeling and experimental validation showed compromised arteriolar network function with diminished flow reserve.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Physiology
Hao Yin, John-Michael Arpino, Jason J. Lee, J. Geoffrey Pickering
Summary: The skeletal muscle is the largest organ in humans, with its viability and performance depending on the integrity of its microcirculation. Peripheral vascular disease in adults can damage the microvasculature and myofibers of the skeletal muscle, while the muscle itself has the capacity to regenerate. Understanding the regeneration and competence of the microvascular network will be critical for regenerative medicine in treating peripheral artery disease (PAD).
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Cell & Tissue Engineering
Frederik Penzien Mamsen, Lea Munthe-Fog, Mikela Karen Mungal Kring, Dominik Duscher, Mikkel Taudorf, Adam J. Katz, Stig-Frederik Trojahn Kolle
Summary: The review highlights the benefits of using scaffolds for ASC delivery, showing improved cell viability, angiogenesis, and wound healing outcomes compared to ASCs alone. The type of scaffold material influences ASC differentiation and therapeutic effects, with natural scaffolds being recommended for fat regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Adriana Mariel Gentile, Said Lhamyani, Maria Mengual Mesa, Francisco Javier Pavon-Moron, John R. Pearson, Julian Salas, Mercedes Clemente-Postigo, Lucia Perez Costillas, Gabriel Olveira Fuster, Rajaa El Bekay Rizky
Summary: This study aims to determine the miRNA network associated with age-associated angiogenic enrichment in transformed adipose tissue (TAT). The results show that the expression of miR-15b-5p and miR-29a-3p is significantly upregulated in the TAT of elderly patients, along with increased expression of VEGFA and other angiogenic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shogo Tamura, Masato Mukaide, Yumi Katsuragi, Wataru Fujii, Koya Odaira, Nobuaki Suzuki, Nagaharu Tsukiji, Shuichi Okamoto, Atsuo Suzuki, Takeshi Kanematsu, Akira Katsumi, Akira Takagi, Katsuhide Ikeda, Jun Ueyama, Masaaki Hirayama, Katsue Suzuki-Inoue, Tadashi Matsushita, Tetsuhito Kojima, Fumihiko Hayakawa
Summary: This study found that PDPN-expressing stromal cells are involved in postnatal bone marrow generation and regulate the integrity of primitive vasculatures in the marrow. This is the first comprehensive study on how PDPN-expressing stromal cells contribute to marrow development and homeostasis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell & Tissue Engineering
Aizhen Chen, Shijie Tang, Jiawei He, Xiangyu Li, Guohao Peng, Haoruo Zhang, Jinghua Chen, Liangwan Chen, Xiaosong Chen
Summary: This study explored the characteristics of human adipose-derived mesenchymal stromal cells' small extracellular vesicles (hADMSC-sEVs) and their role in improving the survival rate of grafted fat. The results showed that hADMSC-sEVs promoted the survival of grafted fats by promoting the formation of new blood vessels, reducing inflammation and fibrosis, which represents a promising advancement in the field of regenerative medicine.
STEM CELL RESEARCH & THERAPY
(2021)
Editorial Material
Cell Biology
Pavel Makarevich, Yu-Chen Hu
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Monakova, Georgy Sagaradze, Nataliya Basalova, Vladimir Popov, Vadim Balabanyan, Anastasia Efimenko
Summary: Idiopathic male infertility is a common issue in developed countries with no specific treatment options. The use of mesenchymal stromal cells (MSCs) and their secretome may be a promising approach to restore male fertility. This study suggests a novel method to evaluate the potency of MSC secretome for idiopathic male infertility treatment by analyzing testosterone production by Leydig cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Maxim Karagyaur, Alexandra Primak, Anastasia Efimenko, Mariya Skryabina, Vsevolod Tkachuk
Summary: This review discusses the possibilities and limitations of gene technologies for obtaining cell models, and provides recommendations for the development and creation of relevant models. It is a useful reference point for novice cell biologists in the rapidly growing field of gene and cell technologies.
Article
Biochemistry & Molecular Biology
Anna V. Izosimova, Marina V. Shirmanova, Vladislav I. Shcheslavskiy, Daria A. Sachkova, Artem M. Mozherov, George V. Sharonov, Elena V. Zagaynova, Diana V. Yuzhakova
Summary: FLIM technology can be used to assess the immune response and metabolic changes of T-cells to tumors. The study found that the fluorescence lifetime parameters of NAD(P)H in lymph nodes are sensitive to tumor development. This research provides a powerful tool for evaluating the immune response to tumor development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Iurii S. I. S. Stafeev, Maria A. Boldyreva, Svetlana S. Michurina, Margarita Yu. Agareva, Arina V. Radnaeva, Mikhail Yu. Menshikov, Yu-Chen Hu, Pavel I. Makarevich, Yelena V. Parfyonova
Summary: This study demonstrates the importance of dietary conditions in preclinical testing of non-viral gene therapy drugs. The HGF/VEGF combined plasmid shows potential participation in ischemic skeletal muscle regeneration by regulating innervation and bioenergetics of muscle. HGF/VEGF combined plasmid has great potential for PAD therapy in conditions of impaired glucose tolerance.
Article
Engineering, Biomedical
Galina A. A. Ryltseva, Alexey E. E. Dudaev, Natalia G. G. Menzyanova, Tatiana G. G. Volova, Natalia A. A. Alexandrushkina, Anastasia Yu. Efimenko, Ekaterina I. I. Shishatskaya
Summary: The needs for biodegradable polymers in modern regenerative medicine are diverse. Restoring the viability of the vascular tree is crucial for preserving the functionality of organs and tissues. Developing vascular implants compatible with blood is an important goal of vascular bioengineering, and creating surfaces with specific biomaterial characteristics can facilitate correct endothelialization.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Svetlana Michurina, Iurii Stafeev, Maria Boldyreva, Vu Anh Truong, Elizaveta Ratner, Mikhail Menshikov, Yu-Chen Hu, Yelena Parfyonova
Summary: This study utilized CRISPR activation technology to enhance the expression of UCP1 and generate safe and efficient adipose-tissue-engineered constructs for transplantation. The results showed the presence of UCP1-positive adipocytes in the transplanted grafts, along with the expression of PGC1 alpha transcription factor and HSL. Furthermore, transplantation of CRISPRa-UCP1-modified adipocytes did not affect glucose metabolism or inflammation in recipient mice. The use of baculovirus vectors and CRISPRa technology is a potential approach to improve cell therapy for non-immunogenic adipocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Georgy Sagaradze, Anna Monakova, Anastasia Efimenko
Summary: Adult stem cells are regulated by their specific microenvironments called stem cell niches, and dysfunction of the niche components can lead to chronic or acute disorders. To address this, regenerative medicine treatments such as gene and cell therapy are being investigated, with a focus on mesenchymal stromal cells (MSCs) and their secretomes. However, the development of MSC secretome-based products is not fully regulated, which has complicated their clinical translation and resulted in failed trials. This review discusses the development of potency assays for MSC secretome-based products, specifically their effects on stem cell niches and spermatogonial stem cell niche.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Vadim Chechekhin, Anastasia Ivanova, Konstantin Kulebyakin, Veronika Sysoeva, Daria Naida, Mikhail Arbatsky, Nataliya Basalova, Maxim Karagyaur, Mariya Skryabina, Anastasia Efimenko, Olga Grigorieva, Natalia Kalinina, Vsevolod Tkachuk, Pyotr Tyurin-Kuzmin
Summary: Hypertension is a major risk factor for cardiovascular diseases and is often linked to obesity. This study investigates the impact of obesity-driven hypertension on the expression and functioning of adrenoceptors in adipose tissue.
Article
Biochemistry & Molecular Biology
Olga Grigorieva, Nataliya Basalova, Maksim Vigovskiy, Mikhail Arbatskiy, Uliana Dyachkova, Maria Kulebyakina, Konstantin Kulebyakin, Pyotr Tyurin-Kuzmin, Natalia Kalinina, Anastasia Efimenko
Summary: MSCs play a dual role in fibrosis, both suppressing the process through paracrine activity and promoting it by differentiating into myofibroblasts. This study identified the potential involvement of neurotrimin and CHD3 in the regulation of myofibroblast differentiation and fibrosis development in human adipose tissue MSCs.
Article
Biochemistry & Molecular Biology
Valentina S. Glazieva, Natalya A. Alexandrushkina, Peter P. Nimiritsky, Maria A. Kulebyakina, Roman Yu. Eremichev, Pavel I. Makarevich
Summary: This study aimed to investigate factors affecting the assembly time of cell sheet (CS). Through testing adipose-derived mesenchymal stromal cells (MSCs) from 14 healthy individuals, it was found that high contents of collagen I and collagen IV were associated with prolonged assembly time. Furthermore, MSCs with shorter lag phase assembled faster, while factors such as age, sex, fibronectin showed no significant correlation with assembly time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Pavel Makarevich, Maria Boldyreva, Stalik Dzhauari, Maxim Karagayur, Yelena Parfyonova
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Ekaterina Slobodkina, Maria Boldyreva, Maxim Karagyaur, Vadim Balabanyan, Zhanna Akopyan, Yelena Parfyonova, Vsevolod Tkachuk, Pavel Makarevich
TISSUE ENGINEERING PART A
(2022)
Article
Cardiac & Cardiovascular Systems
A. G. Sorokina, A. Yu Efimenko, O. A. Grigorieva, E. S. Novoseletskaya, N. A. Basalova, N. A. Aleksandrushkina, M. A. Vigovskiy, K. H. Kirillova, I. D. Strazhesko, A. Orlov, A. Balatskiy, L. M. Samokhodskaya, N. Danilova, U. D. Dychkova, A. A. Akopyan, V. V. Kakotkin, D. A. Asratyan, Z. A. Akopyan, Y. A. Orlova
Summary: This study investigates the correlation between vascular wall stiffness and markers for accumulation of senescent cells in the blood, cells, and tissues of elderly patients. The results show that pulse wave velocity (PWV), a measure of arterial wall stiffness, is significantly correlated with the accumulation of senescent cells. Factors such as insulin-like growth factor 1 (IGF-1), fibroblast growth factor 21 (FGF-21), and vascular endothelium adhesion molecule 1 (VCAM-1) in the blood plasma are identified as markers for aging. PWV is suggested as a candidate parameter for evaluating and monitoring biological age during senolytic therapy.