Article
Chemistry, Applied
Rajesh P. Shastry, Saptami Kanekar, Aleema Suzna Pandial, P. D. Rekha
Summary: Isoeugenol is shown to be an effective inhibitor of quorum sensing regulated phenotypes and biofilm formation in Pseudomonas aeruginosa, with more than 70% inhibition of virulence factors. The presence of isoeugenol did not affect planktonic cells, but significantly inhibited biofilm formation through binding interactions with quorum sensor regulators. This suggests the potential of isoeugenol as a blocker of quorum sensing and an antivirulence compound.
NATURAL PRODUCT RESEARCH
(2022)
Article
Microbiology
Xiaofeng Han, Mengyue Nan, Xinyu Cai, Boling Qiao, Lin Chen, Lixin Shen
Summary: Sennoside A can inhibit the cell density-dependent quorum sensing system of Pseudomonas aeruginosa, attenuating its virulence and pathogenicity. This study is of great significance for finding drugs targeting quorum sensing to treat Pseudomonas aeruginosa infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Christian Emmanuel Mahavy, Adeline Mol, Blandine Andrianarisoa, Pierre Duez, Mondher El Jaziri, Marie Baucher, Tsiry Rasamiravaka
Summary: Lutein-type compounds can inhibit biofilm formation and invasion capabilities of Pseudomonas aeruginosa, reduce the production of virulence factors, and enhance the bactericidal activity of antibiotics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yang Yuan, Xiting Yang, Qianglin Zeng, Heyue Li, Ruyi Fu, Lianming Du, Wei Liu, Yamei Zhang, Xikun Zhou, Yiwen Chu, Xiuyue Zhang, Kelei Zhao
Summary: This study reports the antivirulence potentials of Dimetridazole and Ribavirin on Pseudomonas aeruginosa and provides structural basis and methodological reference for the development of anti-pseudomonal drugs. The two compounds significantly inhibit the growth and production of virulence factors in P. aeruginosa, and enhance the susceptibility of drug-resistant strains to existing antibiotics. Moreover, they effectively protect animal models from P. aeruginosa infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Diletta Collalto, Giulia Giallonardi, Alessandra Fortuna, Carlo Meneghini, Ersilia Fiscarelli, Paolo Visca, Francesco Imperi, Giordano Rampioni, Livia Leoni
Summary: This study investigated the effects of niclosamide and clofoctol on 100 P. aeruginosa CF isolates, finding variable and strain-dependent inhibition of QS and virulence. The study highlights the importance of testing new antivirulence drugs against large panels of CF clinical isolates and supports the development of pqs inhibitors for antivirulence therapy in CF, despite the occurrence of strains naturally resistant to QS inhibitors.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Dan Yang, Suqi Hao, Ling Zhao, Fei Shi, Gang Ye, Yuanfeng Zou, Xu Song, Lixia Li, Zhongqiong Yin, Xiaoli He, Shiling Feng, Helin Chen, Yu Zhang, Yuanze Gao, Yinglun Li, Huaqiao Tang
Summary: The study found that paeonol has good anti-biofilm activity against Gram-negative bacteria and anti-QS activity, effectively reducing the virulence of P. aeruginosa. In vivo studies showed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Marketa Macho, Subhasish Saha, Grzegorz Konert, Avik Banerjee, Daniela Ewe, Pavel Hrouzek, Petra Urajova, Kumar Saurav
Summary: This study screened the MMV Pandemic Response Box (PRB) and identified four compounds with significant inhibition of P. aeruginosa virulence factor production and biofilm formation, providing an opportunity for the development of new antibacterial drugs.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Hyeon-Ji Hwang, Heejeong Choi, Sojeong Hong, Hyung Ryong Moon, Joon-Hee Lee
Summary: By screening a synthetic chemical library, two compounds, MHY1383 and MHY1387, were found to have both antivirulence and antibiofilm activities against Pseudomonas aeruginosa at extremely low concentrations. These compounds show significant anti-QS effects at 100 pM and antibiofilm effects at 1 to 10 pM, offering promise as potential antipathogenic and antibiofilm drugs with few adverse side effects.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Ying Zhang, Lingbo Wang, Liqiong Chen, Peiwu Zhu, Na Huang, Tao Chen, Lijiang Chen, Zhongyong Wang, Wenli Liao, Jianming Cao, Tieli Zhou
Summary: This study reveals that PtrA plays a dual regulatory role in the virulence systems of Pseudomonas aeruginosa, negatively regulating the T3SS and positively regulating the QS. The absence of ptrA leads to a weakened extracellular virulence related to QS and affects the carbapenems resistance of PA.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Vijay K. K. Singh, Marianna Almpani, Kelsey M. M. Wheeler, Laurence G. G. Rahme
Summary: Pseudomonas aeruginosa is a common multidrug-resistant bacterial pathogen that poses a serious threat to critically ill and immunocompromised patients. Intestinal colonization by this pathogen is associated with increased mortality rates. In this study, the role of the three major communication systems in P. aeruginosa was investigated, and it was found that the MvfR system plays a key role in intestinal inflammation while the other systems have relatively minor effects. This study reveals the interrelationships of the quorum sensing systems during infection and provides important insights for developing effective antivirulence strategies.
Article
Microbiology
Gertrudis Horna, Joaquim Ruiz
Summary: Pseudomonas aeruginosa is an opportunistic pathogen that mainly affects severe patients in ICUs, characterized by high levels of antibiotic resistance and a long battery of virulence factors. Among these factors, T3SS plays a key role in injecting effectors into host cells and influencing immune responses to increase bacterial survival rates. Despite extensive research on T3SS, there are still questions and further points to be clarified or established. Efforts are being made to develop T3SS-targeting molecules to improve patient outcomes.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Cansu Kaya, Isabell Walter, Samir Yahiaoui, Asfandyar Sikandar, Alaa Alhayek, Jelena Konstantinovic, Andreas M. Kany, Jorg Haupenthal, Jesko Koehnke, Rolf W. Hartmann, Anna K. H. Hirsch
Summary: Extracellular virulence factors, including the LasB enzyme secreted by the Gram-negative pathogen Pseudomonas aeruginosa, have become important targets in the current antimicrobial resistance crisis. In this study, a sub-micromolar, non-peptidic inhibitor of LasB was discovered through structural data analysis and optimization, showing improved in vivo efficacy in Galleria mellonella larvae. This class of compounds shows promise as potential drug candidates for combating infections.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Plant Sciences
Muzamil Ahmad Rather, Kuldeep Gupta, Manabendra Mandal
Summary: The study evaluated the inhibitory effect of methanol extract of Cuphea carthagenensis on biofilm and quorum sensing (QS) activity in Pseudomonas aeruginosa, demonstrating a significant suppression of biofilm formation and virulence factors.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Prasanth Rathinam, Bhasker Mohan Murari, Pragasam Viswanathan
Summary: The study developed silicone segments coated with an antivirulence agent, eugenol, which effectively prevented biofilm formation and bacterial adhesion by inhibiting virulence gene expression. Drug release profiles and toxicity analysis confirmed the effectiveness and safety of the eugenol-coated segments.
Article
Microbiology
Mengmeng Cheng, Ruiyi Chen, Lisheng Liao
Summary: This study investigates the impact of a gene variation, LasR(Q45stop), in Pseudomonas aeruginosa. The introduction of LasR(Q45stop) in the bacteria led to the development of elastase-phenotypic-variability variants during co-evolution with the wild-type. The variation in T2SS-peptidase xcpA and mexT genes played a crucial role in the divergence of various phenotypes. These findings highlight the ongoing microevolution of individual mutational trajectory diversity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Huafu Wang, Faping Tu, Zhihong Gui, Xianghong Lu, Weihua Chu
INDIAN JOURNAL OF MICROBIOLOGY
(2013)
Article
Biotechnology & Applied Microbiology
Zhihong Gui, Huafu Wang, Ting Ding, Wei Zhu, Xiyi Zhuang, Weihua Chu
INDIAN JOURNAL OF MICROBIOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Zhenqiu Shang, Huafu Wang, Shuxin Zhou, Weihua Chu
INDIAN JOURNAL OF MICROBIOLOGY
(2014)
Article
Medicine, Research & Experimental
Huafu Wang, Huang Gang, Shuxin Zhou, Lixian Liu, Ting Ding, Zhihong Gui, Weihua Chug
M S-MEDECINE SCIENCES
(2018)
Article
Oncology
Shanqiang Qu, Zhixin Chen, Bin Liu, Jin Liu, Huafu Wang
Summary: By analyzing clinical data, a prognostic signature with nine m(6)A-related genes was identified in gliomas. The risk score based on these genes is closely associated with clinicopathological features of glioma patients and can effectively predict their survival probability.
Article
Immunology
Huafu Wang, Xianjin Qin, Zhihong Gui, Weihua Chu
MICROBIAL PATHOGENESIS
(2020)
Article
Oncology
Shanqiang Qu, Shuhao Liu, Weiwen Qiu, Jin Liu, Huafu Wang
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2020)