Article
Agriculture, Multidisciplinary
You Wu, Jiaojiao Li, Wenjiao Ding, Zheng Ruan, Li Zhang
Summary: The combination of puerarin with tryptophan or its metabolites can improve the intestinal barrier by enhancing the mucus layer barrier, mainly achieved by increasing the number of goblet cells and promoting the secretion of MUC2.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Shuang Qu, Yinghui Zheng, Yichun Huang, Yicheng Feng, Kunyao Xu, Wei Zhang, Yawen Wang, Kaili Nie, Meng Qin
Summary: Disorders in the gut microbiota can damage the intestinal barrier, leading to intestinal disease. In this study, we investigated the effect of excess colonization of Akkermansia muciniphila (A. muciniphila) on the intestinal barrier using a mouse model. The over-colonization of A. muciniphila resulted in decreased intestinal mucin content and reduced expression of tight junction proteins, ultimately damaging the intestinal barrier and exacerbating colitis and colorectal cancer development. These findings have important implications for the safety of A. muciniphila as a probiotic.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Guo-Lian Gan, Hua-Tao Wu, Wen-Jia Chen, Chun-Lan Li, Qian-Qian Ye, Yi-Feng Zheng, Jing Liu
Summary: Abnormal expression patterns of MUC2 have been observed in various malignant tumors and precancerous lesions. In patients with CRC, MUC2 expression in tissues is lower while serum levels are higher compared to normal controls. MUC2 expression is associated with TNM stage, tumor type, distant metastasis, and biomarkers of intestinal mucosal barrier function in CRC patients.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Multidisciplinary Sciences
Adnan Islam, Joshua L. Wheatley, Susana Chavez-Bueno
Summary: Newborns ingest maternal E. coli strains that can colonize their intestinal tract and cause life-threatening bacteremia. This study presents a method using polarized intestinal epithelial cells to assess the transcytosis of neonatal E. coli bacteremia isolates. The method involves growing T84 intestinal cell line on semipermeable inserts and quantifying transepithelial resistance (TEER) to measure paracellular permeability and bacterial passage.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Nutrition & Dietetics
Siwen Lyu, Qi Yang, Xuehui Duan, Xuanting Liu, Zhiyang Du, Xiaomin Shang, Menglei Xu, Jingbo Liu, Fengguang Pan, Ting Zhang
Summary: Fermented egg-milk peptides (FEMPs) were found to enhance the colon-intestinal barrier and regulate multiple kinase-related targets. Additionally, FEMPs were involved in the kinase activity of metabolic processes and played a role in important procedures such as cell apoptosis. By studying the interaction between peptides and targets in a multi-target and multi-pathway pattern, this research provided a new perspective on the functional validation and potential application of bioactive peptides.
FRONTIERS IN NUTRITION
(2022)
Article
Agriculture, Multidisciplinary
Lei Du, Yi-Ming Hao, Yu-Hong Yang, Yan Zheng, Zi-Jian Wu, Meng-Qing Zhou, Bao-Zhen Wang, Yu-Ming Wang, Hao Wu, Guo-Hai Su
Summary: DHA-PL and EPA-PL maintain intestinal barrier integrity by balancing apoptosis and autophagy in intestinal epithelial cells, and alleviate LPS-induced intestinal barrier injury by inhibiting NF-κB and MAPKs pathways activation and activating Nrf2 antioxidant pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Immunology
Akane Hayashi, Naoaki Sakamoto, Koji Kobayashi, Takahisa Murata
Summary: Pharmacological DP1 stimulation decreases intestinal permeability by stimulating mucus secretion.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Zhi-Yuan Lu, Lin Feng, Wei-Dan Jiang, Pei Wu, Yang Liu, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Shu-Wei Li, Cheng-Bo Zhong, Xiao-Qiu Zhou
Summary: This study evaluated the effect of MOS supplementation on the immune barrier function of grass carp and found that it enhances immunity, attenuates inflammatory response, and regulates immune barrier function through specific signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Ilinca Iozon, Mihai Cernea, Victoria Buza, Viorel Miclaus, Maria-Catalina Matei Latiu, Cristian Martonos, Ion Vlasiuc, Andrei Radu Szakacz, Laura Cristina Stefanut
Summary: This study aimed to evaluate the effects of long-term oral inulin administration on intestinal mucosa structure and functions. The results showed that direct contact between inulin and intestinal mucosa did not cause any irritations, inflammations or functional alterations.
PAKISTAN VETERINARY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Zehua Huang, Huimin Wu, Junjie Fan, Qixiang Mei, Yang Fu, Nuoming Yin, Binqiang Xu, Shengzheng Luo, Baiwen Li, Jianbo Ni, Chunlan Huang, Junjie Hu, Yue Zeng
Summary: MUC2 secreted by goblet cells participates in the intestinal barrier and its deficiency aggravates pancreatic and colonic injuries, goblet cell dysfunction, gut dysbiosis, and bacterial translocation in acute necrotizing pancreatitis (ANP) rats. MUC2 supplementation can relieve these phenomena and partially reverse goblet cell dysfunction. Downregulating MUC2 expression leads to increased levels of pro-inflammatory cytokines in colonoids.
Article
Chemistry, Multidisciplinary
Xinyuan Yang, Jiali Yang, Zihan Ye, Guizhen Zhang, Weimin Nie, Hui Cheng, Mengyun Peng, Kaixiang Zhang, Junjie Liu, Zhenzhong Zhang, Jinjin Shi
Summary: Coating probiotics with tannic acid and mucin can enhance their survival and colonization in the gastrointestinal tract, leading to potential applications in disease prevention and treatment.
Article
Polymer Science
Kayo Terada, Taichi Kurita, Joan Gimenez-Dejoz, Hiroyasu Masunaga, Kousuke Tsuchiya, Keiji Numata
Summary: His-containing polypeptides with repetitive sequences were polymerized using chemoenzymatic methods, resulting in polypeptides with different structures and thermal stability.
Article
Nanoscience & Nanotechnology
Kedong Liu, Yun Chen, Dutao Yang, Yanfei Cai, Zhaoqi Yang, Jian Jin
Summary: Developing a hybrid micelle platform for oral drug delivery, with the ability to overcome both mucus permeation and epithelium absorption barriers, was investigated in this study. The optimized hybrid micelle exhibited excellent mucus and villi penetration capabilities, as well as enhanced cellular uptake. The results also indicated the involvement of the retrograde pathway in the intracellular transport and transcytosis delivery of the hybrid micelles.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Xinyue Wang, Sisi Lin, Lu Wang, Zhenping Cao, Mengmeng Zhang, Yifan Zhang, Rui Liu, Jinyao Liu
Summary: Outer membrane vesicles (OMVs) released by Akkermansia muciniphila can restore gut microbiota balance, stimulate mucosal immunoglobulin A response, and maintain intestinal barrier integrity by entering intestinal epithelial cells. Transplanting gut microbiota-derived OMVs can alleviate colitis and enhance anti-PD-1 therapy against colorectal cancer. This study highlights the important role of gut microbiota-derived OMVs in intestinal ecology and provides a new target for disease intervention and treatment.
Review
Chemistry, Multidisciplinary
Sarfaraz Ahmad Ejazi, Rebecca Louisthelmy, Katharina Maisel
Summary: Oral drug administration is popular but faces challenges in the harsh gastrointestinal environment. Nanoparticles can enhance drug bioavailability by protecting against degradation, promoting absorption, and facilitating drug transport across the intestinal epithelium. This review focuses on the components of intestinal barriers, various nanoparticles developed for oral delivery, and the intracellular pathways involved in nanoparticle internalization and transport. Understanding these factors may lead to the development of more effective nanoparticles for drug delivery.
Article
Nutrition & Dietetics
Valeria Dipasquale, Katia Lecoeur, Madeleine Aumar, Dominique Guimber, Stephanie Coopman, Audrey Nicolas, Audrey Lecoufle, Audrey Van Malleghem, Dominique Turck, Delphine Ley, Frederic Gottrand
Summary: This study examined the tube-weaning practice of a group of pediatric gastroenterologists managing children receiving prolonged enteral nutrition. The results showed significant practice variation, with most respondents using individualized weaning programs and preferring home-based weaning attempts. The study highlights the need for standardized tube-weaning programs based on increased knowledge about different weaning modalities.
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
(2022)
Article
Pediatrics
Aurelie Pham, Emmanuelle Ecochard-Dugelay, Arnaud Bonnard, Enora Le Roux, Thomas Gelas, Veronique Rousseau, Nadege Thomassin, Isabelle Cabon-Boudard, Audrey Nicolas, Audrey Guinot, Julie Rebeuh, Aurelie Le Mandat, Djamal-Dine Djeddi, Virginie Fouquet, Aurelie Boucharny, Sabine Irtan, Julie Lemale, Aurelie Comte, Laure Bridoux-Henno, Claire Dupont-Lucas, Georges Dimitrov, Anne Turquet, Corinne Borderon, Cecile Pelatan, Emilie Chaillou Legault, Camille Jung, Stephanie Willot, Louise Montalva, Delphine Mitanchez, Frederic Gottrand, Marc Bellaiche
Summary: Feeding disorders are common in children with oesophageal atresia, with no typical profile of patients at risk. A national cohort study in France found 42% of children experienced feeding disorders, and systematic screening for these disorders is recommended to improve patient care and outcomes.
ARCHIVES OF DISEASE IN CHILDHOOD
(2022)
Article
Polymer Science
Safa Ouerghemmi, Stephanie Degoutin, Mickael Maton, Nicolas Tabary, Frederic Cazaux, Christel Neut, Nicolas Blanchemain, Bernard Martel
Summary: This study focuses on the preparation of core-sheath nanofibers using chitosan and cyclodextrin polymer, and investigates the stability, release behavior, and antibacterial activity of triclosan-loaded nanofibers in aqueous medium. The results indicate that the type of nanofibers directly affects the release and antibacterial activity of triclosan.
Article
Biology
Camille Quenon, Thierry Hennebelle, Jean-Francois Butaud, Raimana Ho, Jennifer Samaillie, Christel Neut, Tamatoa Lehartel, Celine Riviere, Ali Siah, Natacha Bonneau, Sevser Sahpaz, Sebastien Antherieu, Nicolas Lebegue, Phila Raharivelomanana, Vincent Roumy
Summary: A preliminary ethnopharmacological survey was conducted in French Polynesia, focusing on the most cited plants used for treating infectious diseases. The study identified Syzygium malaccense as a potential plant species for further analysis, which led to the discovery of seven alkyl-salicylic acids described for the first time in this species. These compounds showed interesting antimicrobial activity against Gram+ bacteria and no toxicity on hepG2 cells. The findings suggest the therapeutic potential of S. malaccense against infectious diseases.
Article
Pediatrics
Agate Bourg, Frederic Gottrand, Benoit Parmentier, Julie Thomas, Anne Lehn, Christian Piolat, Arnaud Bonnard, Rony Sfeir, Julie Lienard, Veronique Rousseau, Myriam Pouzac, Agnes Liard, Philippe Buisson, Aurore Haffreingue, Louis David, Sophie Branchereau, Veronique Carcauzon, Nicolas Kalfa, Marc -David Leclair, Hubert Lardy, Sabine Irtan, Francois Varlet, Thomas Gelas, Diana Potop, Marie Auger-Hunault
Summary: This study evaluated the outcomes of long gap esophageal atresia (EA) compared to non-long gap EA/tracheo-esophageal fistula (TEF) at the ages of 1 and 6 years. The study also assessed whether the initial treatment (delayed primary anastomosis of native esophagus vs. esophageal replacement) influenced mortality and morbidity at these ages. The results showed that the long gap EA group had more complications and longer hospital stay at age 1 year, and digestive complications were more frequent in the long gap group at age 6 years. The study concluded that long gap strongly influenced digestive morbidity at age 6 years.
JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Pharmacology & Pharmacy
S. Moutaharrik, A. Maroni, C. Neut, C. Dubuquoy, L. Dubuquoy, A. Foppoli, M. Cerea, L. Palugan, F. Siepmann, J. Siepmann, A. Gazzaniga
Summary: A novel drug delivery system based on multiple drug release triggers was developed and evaluated for oral colon delivery, with a specific focus on inflammatory bowel disease (IBD) therapy. The system demonstrated pulsatile drug release in different release media and faster drug release in simulated colonic fluid with fecal bacteria from IBD patients. Promising results were observed in a preliminary rat study, indicating potential therapeutic effects on colitis progression and microbiota alteration.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Kevin Gillois, Camille Rivard, Cecile Levasseur-Garcia, Valerie Bezirard, Helene Terrisse, Renaud Leonard, Catherine Robbe-Masselot, Emmanuelle Maguin, Mathias L. Richard, Vassilia Theodorou, Marie-Helene Ropers, Muriel Mercier-Bonin, Herve Robert
Summary: This study evaluated the biodistribution and gut toxicity of two commercially available colloidal silver products, Mesosilver (TM) and AgC, in male mice. Both products had similar silver accumulation after high-dose sub-chronic exposure. Physicochemical modifications of silver nanoparticles and ions were observed in the gut. Both products had a moderate impact on the gut barrier at lower doses. The composition of gut microbiota was altered differently by each product. Near-infrared spectroscopy successfully distinguished between the two exposures. This study demonstrates the applicability of biophysics-based methods in understanding the uptake, fate, and toxicological effects of colloidal silver after oral exposure.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Nathalia Oderich Muniz, Sarah Gabut, Mickael Maton, Pascal Odou, Michele Vialette, Anthony Pinon, Christel Neut, Nicolas Tabary, Nicolas Blanchemain, Bernard Martel
Summary: The search for effective protective masks against the 2019 coronavirus outbreak and air pollution has led to the development of advanced filtering textiles with antimicrobial properties. This study successfully produced a filtering electrospun membrane that combines optimal filtration efficiency with fast deactivation of viruses and bacteria. The membrane, composed of polyvinyl alcohol nanofibers stabilized with a biocidal agent, showed promising results against various pathogens, including a human coronavirus strain.
Article
Nutrition & Dietetics
Lucie Marousez, Lea Chantal Tran, Edwina Micours, Matthieu Antoine, Frederic Gottrand, Jean Lesage, Delphine Ley
Summary: Inadequate perinatal nutrition can affect the maturity of the intestinal barrier and the development of the intestinal microbiota in mice. Supplementing with prebiotic fiber appears to improve gut microbiota establishment in postnatal-growth-restricted mice and can lead to changes in intestinal morphology and microbial composition.
Review
Gastroenterology & Hepatology
Paolo Lionetti, Eytan Wine, Rinat Ran Ressler, Gerard J. Minor, Giles Major, Boutaina Zemrani, Frederic Gottrand, Claudio Romano
Summary: Children who require enteral nutrition often experience gastrointestinal symptoms. Fiber-containing enteral formulas can improve bowel function, promote healthy gut microbiota growth, and improve immune homeostasis. However, there is a lack of guidance in clinical practice.
EXPERT REVIEW OF GASTROENTEROLOGY & HEPATOLOGY
(2023)
Article
Polymer Science
Henry Chijcheapaza-Flores, Nicolas Tabary, Feng Chai, Mickael Maton, Jean-Noel Staelens, Frederic Cazaux, Christel Neut, Bernard Martel, Nicolas Blanchemain, Maria Jose Garcia-Fernandez
Summary: This study aims to combine the properties of hydrogels based on chitosan (CHT) and the polymer of beta cyclodextrin (PCD) for local delivery of cinnamaldehyde (CN) in diabetic foot ulcers. The results demonstrated the successful development of a cytocompatible (ISO 10993-5) injectable hydrogel with antibacterial and antibiofilm activity. Furthermore, a partial active molecule release and an increase in hydrogel elasticity were observed in the presence of CN.
Article
Biochemistry & Molecular Biology
Julie Dubois-Chevalier, Celine Gheeraert, Alexandre Berthier, Clemence Boulet, Vanessa Dubois, Loic Guille, Marie Fourcot, Guillemette Marot, Karine Gauthier, Laurent Dubuquoy, Bart Staels, Philippe Lefebvre, Jerome Eeckhoute
Summary: Cell identity is controlled by a core transcriptional regulatory circuitry (CoRC) consisting of cell-specific transcription factors (TFs). However, our study reveals that the transcriptional regulatory network regulating hepatocyte identity is more complex than initially thought. We identified a set of TFs, which we call hepatocyte identity (Hep-ID)(CONNECT) TFs, that not only control hepatocyte identity effector genes, but also interact with TFs of the CoRC. Furthermore, we found that Hep-IDCONNECT TFs play a role in fine-tuning CoRC TF expression and can reset CoRC TF expression in dedifferentiated hepatocytes.
Article
Medicine, General & Internal
Miguel Seral-Cortes, Sergio Sabroso-Lasa, Marcela Gonzalez-Gross, Carlos Quesada-Gonzalez, Peter Stehle, Frederic Gottrand, Ascension Marcos, Ligia Esperanza-Diaz, Yannis Manios, Odysseas Androutsos, Kurt Widhalm, Denes Molnar, Inge Huybrechts, Manon Muntaner, Aline Meirhaeghe, Diego R. Salazar-Tortosa, Jonatan Ruiz, Luis Mariano Esteban, Idoia A. Labayen, Luis Moreno, HELENA Study Grp
Summary: The study aimed to develop an ALT-specific genetic risk score to evaluate hepatic damage risk in European adolescents. The unweighted and weighted genetic risk scores were found to be significantly associated with ALT levels, and the predictive ability was further improved by integrating BMI as a clinical factor.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Chemistry, Medicinal
Morgane Rivoal, Laurent Dubuquoy, Regis Millet, Natascha Leleu-Chavain
Summary: RIPK2 is an essential downstream signaling molecule that activates various immune receptors, playing a crucial role in the release of pro-inflammatory cytokines. It has emerged as a potential therapeutic target for inflammatory diseases and cancer. This Perspective provides an overview of RIPK2 function, its role in diseases, available inhibitors, therapeutic strategies, and current challenges and outlook.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Florine Ruthmann, Nadia Guerouaou, Francis Vasseur, Maria-Claire Migaud, Dominique Deplanque, Frederic Gottrand, Laurent Beghin, Odile Viltart
Summary: Recent studies have found associations between emotional disorders, cognitive impairments, and cardiovascular disorders at the subclinical level. This exploratory study aimed to investigate these relationships in the general population. The results suggest that anxiety and depression are differentially related to cognitive and endothelial functions, but the relationship between cognitive performance and endothelial function was not significant.