Article
Infectious Diseases
Aliaksandra Aniskevich, Iryna Shimanskaya, Iryna Boiko, Tatyana Golubovskaya, Daniel Golparian, Iryna Stanislavova, Susanne Jacobsson, Aliaksandr Adaskevich, Magnus Unemo
Summary: This study in Belarus found high levels of resistance to ciprofloxacin and tetracycline in Neisseria gonorrhoeae isolates, while resistance to azithromycin was low and no resistance to ceftriaxone was identified. Ceftriaxone 1g can continue to be recommended as the first-line therapy for gonorrhoea in Belarus, with emphasis on timely updating and adherence to national treatment guidelines based on antimicrobial resistance data.
BMC INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Samantha G. Palace, Kyra E. Fryling, Ying Li, Adam J. Wentworth, Giovanni Traverso, Yonatan H. Grad
Summary: Novel therapeutic strategies are urgently needed for the treatment of Neisseria gonorrhoeae infections due to increasing antimicrobial resistance. Local delivery of bile acids and short- and medium-chain fatty acids shows promise as rapid bactericidal agents against N. gonorrhoeae in oropharyngeal infections, presenting potential for further development as anti-gonococcal agents.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Immunology
Sara Morselli, Valeria Gaspari, Alessia Cantiani, Melissa Salvo, Claudio Foschi, Tiziana Lazzarotto, Antonella Marangoni
Summary: The study found a negative association between Neisseria meningitidis and Neisseria gonorrhoeae in a cohort of men having sex with men, with meningococcal carriage being more common in younger individuals.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jolein Gyonne Elise Laumen, Said Abdellati, Christophe Van Dijck, Delphine Martiny, Irith De Baetselier, Sheeba Santhini Manoharan-Basil, Dorien van den Bossche, Chris Kenyon
Summary: This article explores the feasibility of using oral rinse-and-gargle samples and selective media to monitor antimicrobial resistance in commensal oropharyngeal Neisseria. The pilot study shows that the diversity and number of Neisseria species isolated from rinse samples were similar to those from swabs. The proportion of Neisseria colonies with decreased susceptibility to azithromycin was also comparable between the two sample types. This study provides encouraging data for using a simple protocol of oral rinse-and-gargle as a surveillance tool for monitoring antimicrobial susceptibility in commensal oropharyngeal Neisseria.
Article
Immunology
Claire C. Bristow, Tatum D. Mortimer, Sheldon Morris, Yonatan H. Grad, Olusegun O. Soge, Erika Wakatake, Rushlenne Pascual, Sara McCurdy Murphy, Kyra E. Fryling, Paul C. Adamson, Jo-Anne Dillon, Nidhi R. Parmar, Hai Ha Long Le, Hung Van Le, Reyna Margarita Ovalles Urena, Nireshni Mitchev, Koleka Mlisana, Teodora Wi, Samuel P. Dickson, Jeffrey D. Klausner
Summary: By analyzing the genome sequencing and phenotypic antimicrobial susceptibility data of global N. gonorrhoeae isolates, it was found that a single genetic marker, gyrA codon 91, can accurately predict susceptibility to ciprofloxacin. However, multiple genetic markers are needed to accurately predict susceptibility to other antimicrobials.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Anne F. Luetkemeyer, Deborah Donnell, Julia C. Dombrowski, Stephanie Cohen, Cole Grabow, Clare E. Brown, Cheryl Malinski, Rodney Perkins, Melody Nasser, Carolina Lopez, Eric Vittinghoff, Susan P. Buchbinder, Hyman Scott, Edwin D. Charlebois, Diane V. Havlir, Olusegun O. Soge, Connie Celum
Summary: In this study, it was found that doxycycline postexposure prophylaxis significantly reduced the incidence of sexually transmitted infections (STIs) among MSM and transgender women. The use of doxycycline was shown to be effective in preventing STIs in this population.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Review
Microbiology
Francis Kakooza, Reuben Kiggundu, Gerald Mboowa, Patrick David Kateete, Olga Tendo Nsangi, Jupiter Marina Kabahita, Bernard Ssentalo Bagaya, Daniel Golparian, Magnus Unemo
Summary: A recent study found that there is very limited data on antimicrobial resistance (AMR) surveillance in Neisseria gonorrhoeae (NG) in Africa. There are significant variations in surveillance protocols, methods, and quality assurance among different studies, making comparisons of AMR data difficult. Therefore, establishing a strengthened, standardized, and quality-assured surveillance system in Africa is crucial for identifying AMR trends, detecting emerging resistance, and refining treatment guidelines. Achieving this goal requires leadership and financial commitments from national and international stakeholders.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Winston E. Abara, Kyle T. Bernstein, Felicia M. T. Lewis, Julia A. Schillinger, Kristen Feemster, Preeti Pathela, Susan Hariri, Aras Islam, Michael Eberhart, Iris Cheng, Alexandra Ternier, Jennifer Sanderson Slutsker, Sarah Mbaeyi, Robbie Madera, Robert D. Kirkcaldy
Summary: The MenB-4C vaccine may provide protection against gonorrhoea.
LANCET INFECTIOUS DISEASES
(2022)
Article
Immunology
Laura T. Phillips, Adam A. Witney, Martina Furegato, Ken G. Laing, Liqing Zhou, S. Tariq Sadiq
Summary: Early versions of nanopore technology show promise as a real-time surveillance tool, capable of accurately identifying closely related Neisseria gonorrhoeae strains and important antimicrobial resistance markers from cultured isolates, with very short sequencing times.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Connor Allen, Christopher K. Fairley, Marcus Y. Chen, Kate Maddaford, Jason J. Ong, Deborah A. Williamson, Eric P. F. Chow
Summary: The study found that oropharyngeal gonorrhoea infection is common among heterosexual women and men with untreated urogenital gonorrhoea. The positivity of oropharyngeal gonorrhoea was higher in women than in men and in individuals who were contacts of gonorrhoea cases. Testing for oropharyngeal gonorrhoea in heterosexual individuals can help identify unrecognized infections and guide appropriate treatment.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Genetics & Heredity
Leonor Sanchez-Buso, Corin A. Yeats, Benjamin Taylor, Richard J. Goater, Anthony Underwood, Khalil Abudahab, Silvia Argimon, Kevin C. Ma, Tatum D. Mortimer, Daniel Golparian, Michelle J. Cole, Yonatan H. Grad, Irene Martin, Brian H. Raphael, William M. Shafer, Katy Town, Teodora Wi, Simon R. Harris, Magnus Unemo, David M. Aanensen
Summary: The study introduces a public health-focused scheme for genomic epidemiology of N. gonorrhoeae, using integrated AMR prediction and summary data to reveal new resistant clones and transmission networks, global expansion of resistant lineages, providing a guided tool for public health surveillance.
Article
Infectious Diseases
Henry J. C. de Vries, Myrthe de Laat, Vita W. Jongen, Titia Heijman, Carolien M. Wind, Anders Boyd, Jolinda de Korne-Elenbaas, Alje P. van Dam, Maarten F. Schim van der Loeff
Summary: The study aimed to evaluate the efficacy of ertapenem, gentamicin, and fosfomycin as alternative treatments for anogenital Neisseria gonorrhoeae infections. The findings showed that single-dose 1000 mg ertapenem is non-inferior to single-dose 500 mg ceftriaxone in gonorrhoea treatment, while 5 mg/kg gentamicin is not non-inferior to ceftriaxone. Ertapenem is a potential effective alternative for anogenital Neisseria gonorrhoeae infections and merits evaluation for ceftriaxone-resistant infections.
LANCET INFECTIOUS DISEASES
(2022)
Review
Microbiology
Marina Radovanovic, Dusan Kekic, Milos Jovicevic, Jovana Kabic, Ina Gajic, Natasa Opavski, Lazar Ranin
Summary: N. gonorrhoeae is becoming resistant to all major classes of antibiotics, posing a global threat to the treatment of gonorrhoea. Slow updates of treatment guidelines and the bacterium's high capacity to develop and retain antimicrobial resistance may lead to worsening global antimicrobial resistance in gonococci.
Article
Immunology
Valeria Gaspari, Marielle Ezekielle Djusse, Sara Morselli, Luca Rapparini, Claudio Foschi, Simone Ambretti, Tiziana Lazzarotto, Bianca Maria Piraccini, Antonella Marangoni
Summary: Commensal Neisseria species in the oropharynx may serve as an important reservoir for multi-drug resistant gonorrhea, particularly in men having sex with men (MSM). We evaluated the antimicrobial susceptibility of non-pathogenic Neisseria species isolated from the oropharynx of MSM attending a sexually transmitted infection clinic in Bologna, Italy, and a general population of males. The study found that MSM had higher rates of resistance to azithromycin and ciprofloxacin compared to the general population, highlighting the potential risk of the spread of multi-drug resistant gonorrhea.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Emily Rowlinson, Olusegun O. Soge, James P. Hughes, Anna Berzkalns, Christina Thibault, Roxanne P. Kerani, Christine M. Khosropour, Lisa E. Manhart, Matthew R. Golden, Lindley A. Barbee
Summary: Recent individual-level azithromycin treatment is associated with subsequent azithromycin-resistant gonococcal infections, possibly due to the long half-life and persistence of subtherapeutic levels of azithromycin resulting in the selection of resistant strains.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Boris Shaskolskiy, Dmitry Kravtsov, Ilya Kandinov, Sofya Gorshkova, Alexey Kubanov, Victoria Solomka, Dmitry Deryabin, Ekaterina Dementieva, Dmitry Gryadunov
Summary: This study performed a comparative whole-genome analysis of different Neisseria gonorrhoeae isolates, revealing specific genes for each sequence type and their association with changes in the gonococcal genetic island (GGI) and antibiotic susceptibility. Moreover, the evolutionary pathways of N. gonorrhoeae may involve changes in adhesion and virulence-associated genes, GGI structure, and genes related to host adaptation or biochemical pathways.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Arfenya E. Karamova, Dmitry A. Verbenko, Anastasiia A. Vorontsova, Maryana B. Zhilova, Alexandr A. Nikonorov, Eugenia R. Gatiatulina, Ludmila F. Znamenskaya, Alexey A. Kubanov
Summary: The aim of this study was to investigate the effects of phototherapy on skin cytokines in patients with mycosis fungoides (MF). The results showed that levels of IL-4 and TNF-alpha were elevated in the lesional skin of MF patients. Following treatment, there were positive correlations between mSWAT and IL-22, IL-33, and TNF-alpha levels in the tumor tissue. Levels of IL-10 and IFN-gamma were increased after PUVA treatment. There was no difference in cytokine levels before and after NB-UVB therapy.
JOURNAL OF ONCOLOGY
(2022)
Article
Microbiology
Ilya Kandinov, Dmitry Gryadunov, Alexandra Vinokurova, Olga Antonova, Alexey Kubanov, Victoria Solomka, Julia Shagabieva, Dmitry Deryabin, Boris Shaskolskiy
Summary: This study found that Neisseria gonorrhoeae strains carrying plasmids exhibited high-level resistance to β-lactam antibiotics. However, these plasmid-carrying strains showed reduced viability, possibly due to the additional reproduction costs of the plasmid and the expression of ESBLs. This finding may explain the absence of ESBL-producing N. gonorrhoeae clinical isolates.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Golikova, Kamilla N. Alieva, Alla Filimonova, Vladimir A. Ageevets, Ofeliia S. Sulian, Alisa A. Avdeeva, Sergey Sidorenko, Stephen H. Zinner
Summary: This study evaluated the minimum inhibitory concentrations (MICs) of two carbapenem/carbapenemase inhibitor combinations against Klebsiella pneumoniae strains at standard inocula (SI) and high inocula (HI). The results showed that the MICs increased at HI compared to SI, and there was a correlation between the MICs at SI and the inoculum-induced MIC changes.
Article
Infectious Diseases
Didem Torumkuney, Roman Kozlov, Sergey Sidorenko, Praveen Kamble, Margarita Lezhnina, Aleksandr Galushkin, Subhashri Kundu
Summary: Russia has implemented a national strategy to prevent the spread of antimicrobial resistance (AMR) and is actively monitoring its implementation. Outpatient antibiotic use is high in Russia, and non-prescription access and self-medication are common. Russia conducts antibiotic susceptibility studies and uses international guidelines to develop local guidelines. Providing more relevant guidelines for Russian clinicians can improve appropriate antibiotic prescribing and adherence, potentially limiting the development of AMR and improving patient outcomes.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Medicine, General & Internal
Alexey A. Kubanov, Arfenya E. Karamova, Vadim V. Chikin, Ekaterina S. Monchakovskaya, Mariya A. Nefedova
Summary: The study found that intradermal injections of allogeneic fibroblasts could effectively treat small non-healing wounds in patients with junctional epidermolysis bullosa. After treatment, the wound area significantly decreased, or achieved 100% re-epithelialization, and the expression of related proteins also improved.
RUSSIAN OPEN MEDICAL JOURNAL
(2022)
Article
Microbiology
Nikita Nosov, Alexey Kubanov, Viktoria Solomka, Dmitry Deryabin
Summary: Many current gonococcal clinical isolates in Russia show atypical biochemical activity, leading to species misidentification. This study analyzed the molecular mechanisms of biochemical atypia in N. gonorrhoeae clinical isolates assigned to the G807 NG-MAST GENOGROUP/ST1594 MLST.
Article
Infectious Diseases
Ilya Kandinov, Boris Shaskolskiy, Dmitry Kravtsov, Alexandra Vinokurova, Sofya Gorshkova, Alexey Kubanov, Victoria Solomka, Julia Shagabieva, Dmitry Deryabin, Ekaterina Dementieva, Dmitry Gryadunov
Summary: The aim of this study was to analyze genetic resistance determinants and study the resistance to macrolides (azithromycin) in the modern Russian population of N. gonorrhoeae. Although azithromycin is not used to treat gonococcal infection in Russia, 13.6% of the 162 collected isolates in 2020-2021 exhibited phenotypic resistance to azithromycin. Mutations in 23S rRNA genes were only found in two isolates, while the erm and mefA genes were absent. Azithromycin resistance was mainly associated with mutations in the mtrR and mtrD genes of the MtrCDE efflux pump, as well as mosaic alleles formed due to horizontal transfer from N. meningitidis. A total of 30 types of mtrR alleles and 10 types of mtrD alleles, including mosaic variants, were identified. The matching between mtrR and mtrD alleles indicated cooperative molecular evolution of these genes. The association between mtrR and mtrD alleles and NG-MAST types was only found in NG-MAST 228 and 807, which are typical of N. gonorrhoeae in Russia. The high level of azithromycin resistance in Russia may be related to the spread of multiple transferable resistance to antimicrobials, regardless of their use in the treatment of gonococcal infection.
Article
Microbiology
Ilya Kandinov, Ekaterina Dementieva, Marina Filippova, Alexandra Vinokurova, Sofya Gorshkova, Alexey Kubanov, Victoria Solomka, Julia Shagabieva, Dmitry Deryabin, Boris Shaskolskiy, Dmitry Gryadunov
Summary: The factors affecting the emergence of azithromycin-resistant Neisseria gonorrhoeae isolates in Russia were determined through the analysis of clinical isolates collected from 2018 to 2021. The emergence of azithromycin resistance in Russia in 2020 was attributed to the spread of European N. gonorrhoeae strains belonging to the G12302 genogroup. A hydrogel DNA microarray was developed to analyze resistance determinants, and it was found that the resistance was associated with specific genetic mutations.
Article
Infectious Diseases
Vladimir Gostev, Olga Kalinogorskaya, Julia Sopova, Ofelia Sulian, Polina Chulkova, Maria Velizhanina, Irina Tsvetkova, Irina Ageevets, Vladimir Ageevets, Sergey Sidorenko
Summary: Vancomycin and daptomycin are commonly used antibiotics for treating methicillin-resistant Staphylococcus aureus infections, but their efficacy is limited by their resistance and associated resistance to each other. The effectiveness of novel lipoglycopeptides in overcoming this associated resistance is still unknown.
Article
Infectious Diseases
Vladimir Gostev, Ksenia Sabinova, Julia Sopova, Olga Kalinogorskaya, Ofeliia Sulian, Polina Chulkova, Maria Velizhanina, Polina Pavlova, Lavrentii Danilov, Lyudmila Kraeva, Dmitrii Polev, Elvira Martens, Sergey Sidorenko
Summary: The aim of this study was to investigate the phenotypic and genetic properties of oxacillin-susceptible methicillin-resistant Staphylococcus aureus (OS-MRSA) isolates and their beta-lactam resistant derivatives. The findings showed that OS-MRSA isolates were more prevalent among community-acquired MRSA than hospital-acquired MRSA. They were characterized by specific genetic mutations and lower levels of mecA expression compared to control MRSA isolates. The study emphasized the importance of accurate detection and appropriate treatment of OS-MRSA in order to prevent resistance development and treatment failures.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Kamilla B. Alieva, Maria N. Golikova, Svetlana A. Dovzhenko, Mikhail A. Kobrin, Elena S. Strukova, Vladimir V. Ageevets, Alisa H. Avdeeva, Ofeliia Sulian, Sergey Sidorenko, Stephen Zinner
Summary: OXA-48 carbapenemases are frequently expressed by Klebsiella pneumoniae clinical isolates and decrease the effectiveness of meropenem therapy. The emergence of resistance in susceptible carbapenemase-producing isolates and their resistance patterns when exposed to meropenem are not completely understood. This study confirmed the applicability of the mutant selection window (MSW) hypothesis to meropenem and carbapenemase-producing K. pneumoniae.
Article
Hematology
A. A. Vorontsova, A. E. Karamova, A. A. Nikonorov, D. A. Verbenko, I. Kozlova, E. R. Nikonorova, A. A. Kubanov
Summary: This study evaluated the effect of NB-UVB and PUVA therapy on the dynamics of cytokine mRNA expression in the affected skin of patients with mycosis fungoides. The results showed that phototherapy had a positive effect on the condition and there were positive correlations between multiple cytokines.
GEMATOLOGIYA I TRANSFUZIOLOGIYA
(2023)
Article
Biochemistry & Molecular Biology
O. E. Khokhlova, I. V. Vladimirov, R. S. Kozlov, I. V. Lazareva, M. V. Edelstein, I. A. Larionova, A. V. Molodtsova, V. A. Avdeeva, N. K. Fursova, S. V. Sidorenko
Summary: This study investigates the mechanisms of antibiotic resistance in pathogens causing pyroinflammatory complications in burn-injury patients. The findings reveal a diverse distribution of resistant strains, some of which carry multiple resistance genes.
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
A. A. Kubanov, V. S. Solomka, M. V. Shpilevaya, D. A. Verbenko, D. G. Deryabin, I. D. Kandinov, E. I. Dementieva, D. A. Gryadunov, B. L. Shaskolskiy
Summary: The study aimed to molecularly type Neisseria gonorrhoeae clinical isolates in Russia to identify epidemiologically significant sequence types carrying a penA gene allele with reduced sensitivity to third-generation cephalosporins. The study revealed the diversity of current N. gonorrhoeae sequence types carrying the mosaic penA gene allele and identified a multi-resistance clone of global public health concern.
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
(2022)