4.7 Article

Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots

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SCIENTIFIC REPORTS
卷 5, 期 -, 页码 -

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NATURE PORTFOLIO
DOI: 10.1038/srep15127

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  1. Special Fund for Forest Scientific Research in the Public Welfare [20140430105]
  2. NSF-China [31200471]
  3. Ministry of Finance of the People's Republic of China [RITFYWZX201208]
  4. China Scholarship Council [201303270006]
  5. CFERN & GENE Award Funds on Ecological Paper
  6. Direct For Biological Sciences
  7. Division Of Environmental Biology [1354741] Funding Source: National Science Foundation

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To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots (FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone) and compared the rates of species discrimination (RSD). We quantified RSD by two DNA barcode combinations (rbcL + matK and rbcL + matK + trnH-psbA) using a monophyly-based method (GARLI). We defined two indexes of closely-related taxa (G(m)/G(t) and S/G ratios) and correlated these ratios with RSD. The combination of rbcL + matK averagely discriminated 88.65%, 83.84% and 72.51% at the local, regional and global scales, respectively. An additional locus trnH-psbA increased RSD by 2.87%, 1.49% and 3.58% correspondingly. RSD varied along a latitudinal gradient and were negatively correlated with ratios of closely-related taxa. Successes of species discrimination generally depend on scales in global FDPs. We suggested that the combination of rbcL + matK + trnH-psbA is currently applicable for DNA barcoding-based phylogenetic studies on forest communities.

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