期刊
SCIENTIFIC REPORTS
卷 5, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/srep12275
关键词
-
资金
- National Natural Science Foundation of China [81222025]
- New Century Excellent Talents Program, Ministry of Education, China [NCET-13-0399]
- Sichuan Bureau of Science, China [2013JQ0042]
- Royal Society/NSFC international collaboration award [IE121459, 81311130143]
New Delhi metallo-beta-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 mu g/ml) and meropenem (MIC, 128 mu g/ml) and belonged to ST3835. bla(NDM-1) was the only carbapenemase gene detected. Strain WCHEC13-8 also had a plasmid-borne AmpC gene (bla(CMY-42)) and two extended-spectrum beta-lactamase genes (bla(CTX-M-15) and bla(SHV-12)). bla(NDM-1) and bla(SHV-12) were carried by a 54-kb IncX3 self-transmissible plasmid, which is identical to plasmid pNDM-HF727 from Enterobacter cloacae. bla(CMY-42) was carried by a 64-kb IncI1 plasmid and bla(CTX-M-15) was located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1). bla(CMY-42) was in a complicated context and the mobilisation of bla(CMY-42) was due to the transposition of ISEcp1 by misidentifying its right-end boundary. Genetic context of bla(NDM-1) in strain WCHEC13-8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla(NDM-1), bla(CTX-M-15), bla(CMY-42) and bla(SHV-12) was identified. IncX3 plasmids may be making a significant contribution to the dissemination of bla(NDM) among Enterobacteriaceae in China.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据