期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 277, 期 -, 页码 634-639出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.09.058
关键词
MicroRNA detection; SplintR ligase; L-HRCA; Hepatocellular carcinoma
资金
- National Natural Science of Foundation of China [21432008, 21877086, 21672165]
- National Major Scientific and Technological Special Project for Significant New Drugs Development [2017ZX09303013]
- Natural Science Foundation of Hubei Province of China [2015CFA090]
- East Lake High-tech Zone 3551 Talents Scheme
MiRNA is reported as an important biomarker candidate and therapeutic target in a variety of diseases and cancers. However, ultrasensitive, straightforward, isothermal, low-cost and credible methods for the detection of miRNA from clinical specimens still remain challenges. Here, we establish an isothermal, highly sensitive and specific method for miRNA analysis based on a novel ligation-promoted hyperbranched rolling circle amplification (L-HRCA). This strategy exhibits 10(3)-fold sensitivity enhancement compared to T4 ligase-assisted targetprimer BRCA. The excellent specificity of L-HRCA allows for discriminating target miRNA from negative miRNAs. This method is applied to analyse the expression level of miR-21 in hepatocellular carcinoma. These results indicated that the L-HRCA strategy will become a promising tool to quantify miRNA in early clinical diagnostics.
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