期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 276, 期 -, 页码 42-47出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.08.078
关键词
DNA-template silver nanocluster; DNAzyme; DNA-enzyme conjugate; Bacterial detection
资金
- National Natural Science Foundation of China [41506094]
- National Key Basic Research Program of China [2014CB643304]
- Science & Technology Basic Research Program of Qingdao [16-5-1-19-jch]
- Qingdao National Laboratory for Marine Science and Technology [017ASTCP-ES02]
The rapid and sensitive detection of bacteria is of great importance for the field of food safety, water quality and clinical diagnosis. Herein, we present a novel DNA-templated fluorescent silver nanoclusters (DNA-AgNCs) based sensing system integrated with magnetic nanoparticles (MNP)-DNAzyme-AChE complex for detection of pathogenic bacterial using Escherichia coli (E. coli) as a target bacteria. The platform takes advantage of a DNA-AgNCs based enzyme-responsive fluorescent signal element and a DNAzyme recognition element, providing an ultrasensitive detection limit of 60 cfu mL(-1), and good specificity to the target bacteria. Moreover, the platform could be developed into a potentially versatile system by simply altering corresponding DNAzyme as the objects recognition probes.
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