期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 194, 期 -, 页码 64-70出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.12.065
关键词
Bimetallic gold silver nanoclusters; Reduced graphene oxide-tetraethylene pentamine; Nonenzymatic immunosensor; Squamous cell carcinoma antigen
资金
- Natural Science Foundation of China [21075052, 21175057, 21375047, 21377046]
- Science and Technology Plan Project of Jinan [201307010]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
- UJN [ts20130937]
A simple nonenzymatic immunosensor was developed for sensitive and selective determination of squamous cell carcinoma antigen (SCC-Ag) based on bimetallic gold-silver nanoclusters (AuAg NCs) and reduced graphene oxide-tetraethylene pentamine (rGO-TEPA). rGO-TEPA was used as substrate material to immobilize antibodies since rGO-TEPA has large surface area and excellent conductivity to enhance the electrochemical response signal. AuAg NCs synthesized through simple mortar grinding route were used as catalytic labels of secondary antibodies; meanwhile, AuAg NCs could efficiently enhance the electron transfer. Under the optimal conditions, the electrochemical immunoassay exhibited a wide linear range of 0.005-20 ng/mL with a low detection limit of 1.3 pg/mL for SCC-Ag. The proposed sensing strategy enriches the electrochemical immunoassays and has a promising application in bioassay analysis. (C) 2013 Elsevier B. V. All rights reserved.
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