Article
Electrochemistry
W. B. S. Machini, A. M. Oliveira-Brett
Summary: New emerging bacterial plant diseases are spreading quickly in various geographical regions, with no effective safety measures identified. A Xylella fastidiosa (Xf) DNA-electrochemical biosensor has been developed for the first time, allowing investigation of the Cu(II)-Xf-dsDNA interaction in situ.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Yao Chen, Yu Zhong, Ji-Xing Ye, Yun Lei, Ai-Lin Liu
Summary: In this study, a sensitive and selective electrochemical DNA biosensor was developed for the determination of a osteosarcoma-related survivin gene. The proposed strategy used a simple and low-cost working electrode, with no need for other substances for electrode or DNA functionalization. The mode of interaction between celestine blue (CB) and DNA was studied, and it was found that the possible mode was intercalation with a binding number of 2 and a binding constant beta of 10(12.87). The label-free electrochemical DNA biosensor showed a good linear relationship towards the target gene and had a low detection limit of 0.046 nM.
Article
Biochemical Research Methods
Majid Arvand, Hoda Ilkhani, Mohamad Reza Ganjali, Akram Pourhabib
Summary: A new Tb complex Tb(QS)3 was used to investigate the interaction between the complex and double-stranded DNA, leading to the design of a new DNA biosensor. The interaction mechanism, primarily intercalative interaction, was elucidated using electrochemical methods such as cyclic voltammetry and differential pulse voltammetry.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Chemistry, Physical
Izabella Brand
Summary: Proteins in the cytoplasm bind selectively and sensitively to DNA, leading to structural changes in both biomolecules. Detection of these changes requires sophisticated analytical methods, with electrochemical detection being particularly suitable. This has laid the foundations for the development of biosensors.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Biophysics
Dongfei Chen, Yanfang Wu, Richard D. Tilley, J. Justin Gooding
Summary: An ultra-sensitive and highly-selective electrochemical biosensor has been developed for rapid detection of DNA methylation in blood, providing a promising method for minimally invasive diagnosis of ovarian cancer.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Rafia Nimal, Didem Nur Unal, Cem Erkmen, Sevinc Kurbanoglu, Muhammad Siddiq, Gokcen Eren, Afzal Shah, Bengi Uslu
Summary: This study investigates the interaction between the phenylpiperidine derivative drug paroxetine and biomolecules using electrochemical, fluorescence spectroscopy, and molecular docking methods. The results show that paroxetine has a strong affinity to bind with double-stranded calf thymus DNA through intercalation binding mode.
BIOELECTROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Anna Banasiak, Nicolo Zuin Fantoni, Andrew Kellett, John Colleran
Summary: Several classes of copper complexes have been found to induce oxidative DNA damage, leading to cell death and showing potential as anticancer agents. By analyzing a new compound library, it was discovered that TPMA complexes are a promising class of DNA damaging agents with tunable activity.
Article
Chemistry, Analytical
Xin Xie, Dan Liu, Weili Wang, Juan Xiang, Minghui Yang, Guokun Liu
Summary: Through studying the non-specific adsorption of biofluids on a bare gold electrode, a simple microelectrode-based sensor was developed for the detection of brain-derived neurotrophic factor (BDNF) in aqueous humor (AH). The sensor showed advantages of simplified preparation, sensitive response, and low sample consumption.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Milan Jovanovic, Katarina Nikolic, Marija Carapic, Mara M. Aleksic
Summary: A comprehensive investigation was conducted to clarify the redox mechanism and binding mode of tyrosine kinase inhibitor erlotinib (ERL) with DNA. The irreversible oxidation and reduction processes of ERL on a glassy carbon electrode were studied using three voltammetric techniques. The interaction between ERL and DNA was examined using a multilayer ct-DNA electrochemical biosensor. The results suggested that intercalation may be the dominant way for ERL binding to DNA compared to minor groove binding.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Mihaela-Cristina Bunea, Teodor Adrian Enache, Victor Constantin Diculescu
Summary: This study investigated the interaction between Bortezomib and dsDNA, and found that the binding of Bortezomib to dsDNA led to morphological changes in the dsDNA double helix, which was identified through changes in the oxidation peaks of guanine and adenine residues. The formation of Bortezomib-guanine adducts was confirmed by control experiments and mass spectrometry. Finally, an interaction mechanism between dsDNA and Bortezomib was proposed, explaining the formation of the Bortezomib redox product-guanine adduct.
Article
Engineering, Chemical
Victoria V. V. Shumyantseva, Tatiana V. V. Bulko, Lyubov E. E. Agafonova, Veronika V. V. Pronina, Lyubov V. V. Kostryukova
Summary: In this study, the electrochemical behavior of antiviral drug umifenovir (Umi) and umifenovir encapsulated in phospholipids micelles (NUmi) was investigated on screen-printed electrodes modified by carbon nanotubes. The study revealed the electro oxidation of Umi around +0.4 V in the concentration range of 50-500 μM (R-2 = 0.992). The interaction between umifenovir (or umifenovir encapsulated in phospholipids micelles), purine, and pyrimidine heterocyclic bases of DNA was characterized by non-overlapping signatures and was likely mediated by electrostatic interactions and groove binding in drug-DNA formed complex.
Article
Chemistry, Analytical
Selva Bilge, Burcu Dogan-Topal, Tugba Taskin Tok, Esen Bellur Atici, Ali Sinag, Sibel A. Ozkan
Summary: This study investigated the interaction mechanism between ibrutinib and dsDNA using electrochemical and molecular docking studies. The results revealed that the interaction between ibrutinib and dsDNA is primarily through hydrogen bonding in a groove-binding mode.
MICROCHEMICAL JOURNAL
(2022)
Article
Biophysics
Richa Pandey, Yang Lu, Erin M. McConnell, Enas Osman, Alexander Scott, Jimmy Gu, Todd Hoare, Leyla Soleymani, Yingfu Li
Summary: DNAzyme-based electrochemical biosensors exhibit excellent sensitivity and specificity for disease diagnosis. This review critically discusses the impact of incorporating DNAzymes in electrochemical biosensing, focusing on the recent advances in developing DNAzyme-based electrochemical biosensors for infectious diseases, cancer, and regulatory diseases. It also addresses the challenges in translating these technologies from labs to clinics and discusses strategies to enhance the performance of existing technologies to create point-of-care electrochemical DNAzyme biosensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Palanisamy Bothammal, Singarayan Michelraj, Ganesh Venkatachalam, Ashutosh Verma, Kalimuthusamy Natarajaseenivasan
Summary: A novel electro-chemical biosensor based on lipopolysaccharide for the rapid and highly sensitive diagnosis of leptospirosis during the acute phase of infection has been reported. The sensor offers a cost-effective and rapid diagnostic alternative, with potential clinical utility.
BIOELECTROCHEMISTRY
(2022)
Article
Engineering, Environmental
Yuhan Chen, Yue Gu, Xiaojuan Yi, He Huang, Yishi Li, Bijun Yang, Shuliang Guo, Lijuan Bai
Summary: In this study, a novel nanozyme-mediated electrochemical aptasensor was developed for tuberculosis (TB) diagnosis. The sensor exhibited excellent peroxidase-like activity and conductivity due to the interaction between MXene and gold nanoparticles. By utilizing a specific reaction structure and signal amplification mechanism, the sensor demonstrated high sensitivity in detecting TB-related antigens, showing great potential in clinical practice.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)