A polyaniline-based optical biosensing platform using an entrapped oxidoreductase enzyme

A novel optical biosensing platform utilizing the unique solubility and chemochromic properties of polyaniline is presented A facile, ion-induced reprecipitation method leads to the entrapment of a chosen oxidoreductase enzyme, which. in the presence of its associated substrate, catalyzes a reversible redox change in the host polymer This change is monitored via the UV-vis absorption and subsequently analyzed to fit a Michaelis-Menten model Here, in vitro prototype devices demonstrate selective sensing of glucose, choline, and uric acid, and the potential to be adapted for use as pat teal-time in vivo monitoring systems is discussed (C) 2010 Elsevier B.V. All rights reserved