期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 148, 期 2, 页码 583-589出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.05.027
关键词
Lactose sensor; AC-EPD of enzymes; beta-Galactosidase; Glucose oxidase; Lactose determination in milk
资金
- Research Fund KU Leuven [GOA/08/007]
- Belgian Federal Science Policy Office (BELSPO) [P6/17]
In this paper, we have investigated the deposition of two enzymes beta-galactosidase (beta-Gal) and glucose oxidase (GOx) using alternating current electrophoretic deposition (AC-EPD) to manufacture a lactose sensor. Using the optimal deposition parameters and optimal testing conditions, the sensor has a sensitivity up to 111 nA/mM mm(2). This sensitivity is high considering the low activity of the enzymes employed in this study (9 units/mg for beta-Gal and 5.6 units/mg for GOx). The sensor has a large linear range up to 14 mM lactose, fast response time (similar to 8 s) and reasonable stability without employing any stabilizers or outer polymer membrane. In addition, it is easy and simple to manufacture, highly reproducible and cheap because low activity enzymes are used. The sensor was tested to determine lactose in milk samples and is demonstrated to be accurate. (C) 2010 Elsevier B.V. All rights reserved.
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