期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 150, 期 1, 页码 211-219出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.07.014
关键词
Silica nanoparticles; Surface chemistry; Fluorescence; Bioconjugation; Affinity sensing; Biotin; Click chemistry
资金
- Deutsche Forschungsgemeinschaft (DFG: Bonn) within Graduate College [GRK-640]
Silica nanoparticles (SiNPs) with an average diameter of 25 nm were prepared by a one-pot method that involves the formation of a silica core that is subsequently covered with a shell functionalized with either azido groups or alkyne groups for use in copper(I)-catalyzed click reactions. Respective triethoxysilane reagents are presented The SiNPs were (a) rendered fluorescent by click conjugation to fluorophores of various colors, and (b) made bioconjugatable by Introducing maleimide groups (that covalently bind thiols) and biotin (a widely used bioaffinity reagent that binds streptavidin) Particles were characterized by transmission electron microscopy, infrared spectroscopy, fluorescence, and light scattering. The fluorescently labeled SiNPs carrying maleimido groups were conjugated to the thiol group of bovine serum albumin (BSA) labeled with a fluorophore. and fluorescence resonance energy transfer was shown to occur between the labeled SiNPs and the labeled BSA This is considered to represent a new approach towards nanoparticle-based fluorescent bioassays. (C) 2010 Elsevier B.V All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据