Article
Chemistry, Analytical
Arezoo Saadati, Fatemeh Farshchi, Mohammad Hasanzadeh, Farzad Seidi
Summary: The study introduced a sensitive, quantitative, and rapid diagnostic method for uric acid using silver nanoparticles (AgNPs), suggesting the potential use of various types of AgNPs for on-site detection of clinical samples and biomarkers with portable devices.
ANALYTICAL METHODS
(2021)
Article
Engineering, Environmental
Seong-Geun Jeong, Dong-Ho Kim, Jingyeong Kim, Ji-Hyun Kim, Sanggeun Song, Chang-Soo Lee
Summary: This study presents a method to achieve automatic multistep digital analysis in a single-sheet 3D paper-based microfluidic analytical device (3D-mu PAD) by controlling the penetration of different wax inks into paper. The methodology allows for sequential fluid delivery and multistep biochemical reactions, which can be integrated with digital assays without the need for external instruments. The potential commercial application of this approach is significant.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Analytical
Amer Charbaji, Hojat Heidari-Bafroui, Constantine Anagnostopoulos, Mohammad Faghri
Summary: The paper presents a simple and cost-effective paper-based microfluidic device for detecting nitrate in water. By incorporating recent advancements in paper-based technology suitable for nitrate detection, the device achieved significant improvement in nitrate detection compared to previous methods.
Article
Chemistry, Analytical
Manassawee Janrod, Monpichar Srisa-Art
Summary: A microfluidic paper-based analytical device (mu PAD) with colorimetric detection was developed for simultaneous determination of urinary oxalate, citrate, and uric acid. The detection methods utilized enzymatic reactions and an indicator displacement assay. The mu PAD showed promising results with linear ranges and low detection limits for the three biomarkers, as well as acceptable accuracy and precision in urine sample testing. This development holds great potential for on-site analysis, providing a simple, fast, affordable, and portable tool for simultaneous determination of the biomarkers.
ANALYTICAL METHODS
(2023)
Article
Biochemical Research Methods
Qinan Ban, Yulong Zhang, Yuxuan Li, Daye Cao, Weifeng Ye, Linsheng Zhan, Daming Wang, Xiaohui Wang
Summary: This study developed a point-of-care microfluidic channel-based device for direct measurement of fibrinogen concentration in whole blood. Rapid diagnosis of fibrinogen concentration is of great importance in bleeding patients, and this device shows potential for assessing the suitability of fibrinogen replacement therapy.
Article
Chemistry, Analytical
Zhou Sha, Chunnan Wang, Rui Ma, Xiaochun Gao, Shuqing Sun
Summary: Uniform and monodisperse quantum dot-encoded magnetic microbeads with Janus structure were synthesized using photopolymerization in a microfluidic device. These microbeads exhibited sharp interfaces and excellent magnetic responses. By using different ratios of quantum dots with blue and red emission peaks, three spectral codes were achieved. The ability to distinguish the encoded microbeads by analyzing their fluorescent images in HSV color space was demonstrated. The immobilization of proteins on the microbeads was achieved by hydrolysis of the anhydride group and subsequent activation of carboxyl groups. The integration of Janus-structured quantum dot-encoded magnetic microbeads and microhole arrays provided a novel platform for multiplex bioanalysis, enabling flexibility, binding kinetics, sensitivity for suspension assay, and fluorescence mapping analysis for conventional biochips. The practical applications of this platform were verified through multiplex immunoassays, achieving a detection limit of 0.07 ng/mL for human IgG.
Article
Chemistry, Analytical
Yin Zhao, Dongjuan Chen, Zhichen Xu, Tao Li, Jiang Zhu, Rui Hu, Guoyong Xu, Ying Li, Yunhuang Yang, Maili Liu
Summary: Fast and simplified detection of HPV using a microfluidic dual-droplet device (M-D3) was developed. The device combined the CRISPR-Cas12a system and multiplexed recombinase polymerase amplification (RPA) assay to simultaneously detect HPV16 and HPV18. The M-D3 platform demonstrated high sensitivity and specificity in detecting HPV DNA, allowing on-chip detection within 30 minutes. The platform was validated using clinical patient samples with HPV infection risk, showing promising results in terms of sensitivity and specificity.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Nandini Debnath, Ludovic Saiveng Live, Mahla Poudineh
Summary: Biomarker detection in whole blood is important for understanding the disease. Current detection techniques have limitations, and there is a need for reliable and adaptable methods for point-of-care testing. We have developed a simple and low-cost protein detection device that can detect biomarkers in whole blood within 15 minutes. This technique has the potential to improve the diagnosis and screening of various diseases.
Article
Biotechnology & Applied Microbiology
J-Pablo Salvador, Thomas Brettschneider, Christian Dorrer, M-Pilar Marco
Summary: This study developed a point-of-care device for the detection of oral anticoagulants. The device is automatic, rapid, sensitive, and disposable, and can assist clinicians in monitoring patients under oral anticoagulant treatment. The study demonstrated that the device has good reproducibility and accuracy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Ana Castanheira, Marilia Barreiros dos Santos, Laura Rodriguez-Lorenzo, Raquel Queiros, Begona Espina
Summary: This study developed a microfluidic system for detecting OA with high sensitivity and a wide linear range, capable of detecting low concentrations of OA in contaminated mussels; the enzyme stability and reusability, as well as the simplicity and low cost of microfluidic systems, make this method commercially attractive.
Article
Chemistry, Analytical
Ke Ning, Huihui Chai, Yanxia Cui, Shiming Wu, Feng Chen, Yuan Li, Ping Qiu, Wei Sun, Ling Yu
Summary: A centrifugal microfluidic chip was designed to test blood beta(2) microglobulins (beta(2) M) levels, with a handhold centrifuge used for solution mixing. An office scanner recorded the reaction results, reducing reliance on expensive scientific equipment. The chip-based test showed a sensitivity of 77.3%, specificity of 82.1%, and efficiency of 80%.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Biotechnology & Applied Microbiology
Shamraja S. Nadar, Pravin D. Patil, Manishkumar S. Tiwari, Dinesh J. Ahirrao
Summary: Low-cost paper-based analytical devices are a generation of portable lab-on-chip designs that provide an innovative platform for on/off-site analysis of target analytes, especially in rural and remote areas. These devices offer exciting fundamental features such as ease of fabrication, rapid operation, and precise interpretations, while incorporating enzymes to improve analytical performance. Additionally, they are compact, portable, easy-to-use, and do not require additional sophisticated equipment.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Electrochemistry
Brenda M. de C. Costa, Sophie Griveau, Fethi Bedioui, Fanny d' Orlye, Jose Alberto F. da Silva, Anne Varenne
Summary: We demonstrate the concept of a reusable 3D printed separation device with integrated electrodes for electrochemical detection. The device was optimized for easy and low-cost fabrication and showed good repeatability and linear response in the tested concentration range.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Tsung-Hsien Wu, Yi-Cheng Tsai, Feng-Chih Kuo, Mel S. Lee, Chih-Chien Hu, Gwo-Bin Lee
Summary: Early detection of rheumatoid factors (RF) and cyclic citrullinated peptide antibodies (anti-CCP) is effective in diagnosing and preventing progression of rheumatoid arthritis (RA). A new dual-target detection approach using a symmetrical microfluidic chip was developed, enabling the detection of these two RA biomarkers within 55 minutes using only 5 μL of serum. This device has the potential to enable rapid clinical diagnosis of this common disease.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Chenxi Zhou, Kang Cui, Yue Liu, Shiji Hao, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: This study utilized Pd@hollow Zn/Co core-shell ZIF67/ZIF8 nanoparticles with superior electrocatalytic performance to detect prostate-specific antigen (PSA), demonstrating the potential of introducing noble metal element Pd into MOF hollow structures to improve electrocatalytic efficiency. The high-performance microfluidic paper-based analytical device (μPAD) constructed in this work allowed for dual-mode ultrasensitive detection of biomarkers such as PSA.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)