期刊
SENSORS AND ACTUATORS A-PHYSICAL
卷 199, 期 -, 页码 136-142出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.sna.2013.05.010
关键词
Impedance sensing; Light-addressable potentiometric sensor (LAPS); Cell growth; Cell metabolism; Cell-based biosensor; Cell-based assay
资金
- National Natural Science Foundation of China [60725102, 31000448, 81027003]
- Key Project of Zhejiang Province [2010C14006]
Light-addressable potentiometric sensor (LAPS) can be used as a sensitive pH-meter with its electrolyte-insulator-semiconductor (EIS) structure. Previous LAPS practices in cell-based assays, which were limited to pH detection of extracellular microenvironment, seemed to be insufficient since the cell physiology is reflected by various parameters, such as energy metabolism and cell morphology. In this paper, the feasibility of monitoring cell growth status based on the same sensor structure of LAPS was investigated and testified. A novel real-time biosensor system was established, which could monitor the cell growth and metabolism synchronously. In the cellular experiment, the normal growth of mouse embryonic fibroblast 3T6 cells and their apoptosis in the presence of heavy metal cadmium (Cd) were investigated. The results showed that the normal growth of cells could lead to an increase of 2.65 in cell index (CI). And, CI decreased from 1.96 in normal growth condition to 0.64 24 h after the treatment of Cd and fell to 0.16 after 48 h. The extracellular acidification rate (ECAR) experienced a similar change to CI. The combination of cellular impedance sensing with LAPS will bring out new opportunities for LAPS to be more utility in cell-based assays, such as cytotoxicity detection and drug evaluation. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据