4.6 Article

Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents

期刊

SENSORS
卷 14, 期 7, 页码 12658-12669

出版社

MDPI AG
DOI: 10.3390/s140712658

关键词

biological warfare agent; BWA; love-wave; sensor acoustic wave; SAW; biosensor; immunosensor; microfluidics; bacteriophage

资金

  1. Spanish Science and Innovation Ministry [TEC2010-21357-C05-04]
  2. National Autonomous University of Mexico

向作者/读者索取更多资源

The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.

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