Article
Chemistry, Analytical
Dayananda Desagani, Shani Kleiman, Teddy Zagardan, Hadar Ben-Yoav
Summary: Three-dimensional-printed lab-on-a-chip (LOC) devices offer cost-efficient and easy-to-handle solutions for particle analysis, allowing for remote diagnosis and monitoring of medical conditions such as inflammation.
Article
Engineering, Electrical & Electronic
Yogesh M. Patel, Ritika Malik, Kedar Khare, Supreet Singh Bahga
Summary: We propose a microfluidic holographic cytometry technique that uses three-dimensional hydrodynamic focusing to accurately visualize, classify, and quantify cells and particles in a mixture. This technique achieves high-resolution holographic imaging without the need for computationally-expensive numerical refocusing used in existing methods. It also prevents cell clustering and can be fabricated at a low cost. The technique shows promise for label-free classification and quantification of infected cells in various applications.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2023)
Review
Chemistry, Analytical
Sheng Yan, Dan Yuan
Summary: The development of microfluidic technologies, particularly microflow cytometry with 3D focusing capabilities, has shown great promise in single-cell analysis, offering a wide range of applications in biology and medicine.
Article
Nanoscience & Nanotechnology
Xiaofei Yuan, Andrew Glidle, Hitoshi Furusho, Huabing Yin
Summary: Traditional two-dimensional fluid control strategies in microfluidic cell sorting have limitations in terms of precision and purity, while devices based on three-dimensional hydrodynamics offer better flow-focusing characteristics but are restricted by arbitrary sample positioning. The 3D hydrodynamic focusing sorting platform designed in this study has advantages in precisely controlling sample velocity and position, showing potential for high-accuracy Raman activated sorting.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Mechanics
M. Lopez, M. G. Cabezas, J. M. Montanero, M. A. Herrada
Summary: In this study, the stability of hydrodynamic focusing to produce microemulsions is investigated experimentally and numerically. It is found that the distance between the feeding capillary and the focusing orifice affects the liquid ejection stability. Confined selective withdrawal enhances the stability of the microdripping mode. Surfactants stabilize the meniscus and promote the transition from microdripping to jetting. The global stability analysis accurately predicts the critical flow rate ratio and the droplet emission frequency.
JOURNAL OF FLUID MECHANICS
(2022)
Article
Biochemical Research Methods
Wenhan Zhao, Xiaopeng Shang, Boran Zhang, Dan Yuan, Binh Thi Thanh Nguyen, Wenshuai Wu, Jing Bo Zhang, Niancai Peng, Ai Qun Liu, Fei Duan, Lip Ket Chin
Summary: This study systematically investigates the flow transition process in the hydrodynamic focusing mechanism, revealing the transition from the squeezed focusing state to the over-squeezed anti-focusing state. The recovery rate of cell analysis can be maintained by limiting the Reynolds number below 30. These findings provide new insights into microfluidic cytometric analyses with various applications in food safety, water monitoring, and healthcare sectors.
Article
Chemistry, Analytical
Zhenna Chen, Beibei Chen, Man He, Bin Hu
Summary: A negative magnetophoresis focusing microchip was developed and coupled to ICP-MS for single-cell analysis, achieving high sample throughput. Study on uptake behaviors revealed that MCF-7 cells exhibited more remarkable heterogeneity when treated with ZnO NPs, with lower uptake content compared to Zn2+.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Lingyan Gong, Chayakorn Petchakup, Pujiang Shi, Pei Leng Tan, Lay Poh Tan, Chor Yong Tay, Han Wei Hou
Summary: A novel microfluidic approach for label-free and continuous-flow monitoring of single spheroid/microcarrier based on electrical impedance spectroscopy is reported, with unique impedance signatures identified for optimal spheroid growth and viability monitoring. The platform offers great versatility for multi-parametric analysis of spheroids/microcarriers at high throughput, and can be integrated into bioreactors for long-term and remote monitoring of biomass and cell quality.
Article
Biochemistry & Molecular Biology
Emanuele Piccinno, Anna Grazia Monteduro, Francesco Dituri, Silvia Rizzato, Gianluigi Giannelli, Giuseppe Maruccio
Summary: The study validated a miniaturized on-chip platform for real-time, non-destructive cell proliferation assays and drug screening, comparing its effectiveness with the traditional 96-well plate assay. Results showed the new platform's efficacy comparable to the standard method, with advantages in reagent consumption, operator time, and costs, potentially accelerating drug development processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Filippo Storti, Silvio Bonfadini, Luigino Criante
Summary: Accurately controlling the position of fluid and particles is crucial for lab-on-a-chip platforms. The development of microfluidic fabrication technology has enabled three-dimensional hydrodynamic focusing, which has revolutionized single-cell analysis systems. However, there is still a lack of a device that meets the requirements of high throughput, compactness, high integrability, and ease of use for biomedical research and clinical applications. In this study, a 3D flow focusing microfluidic device buried in fused silica substrate was proposed, which shows promising advantages in terms of focusing capability. High accuracy and resolution were achieved in the detection of particles and bacteria.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Chengxin Wu, Xing Wei, Xue Men, Yulong Xu, Junjie Bai, Yu Wang, Lei Zhou, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: We constructed an open and low-cost flow cytometer that integrates single cell aligning and fluorescence detection functions. The hardware cost for the LIF detection unit and 3D focusing device is $3200 and $400 respectively. The flow cytometer showed favorable assay precision and accuracy, and was successfully applied for evaluating ROS generation in single HepG2 cells.
Article
Pharmacology & Pharmacy
Roni Sverdlov Arzi, Asaf Kay, Yulia Raychman, Alejandro Sosnik
Summary: Nanoprecipitation and microfluidics technology are effective methods for producing pure drug nanoparticles and optimizing product performance. The use of specific microfluidic devices can lead to the production of rounded, optimized nanoparticles.
Review
Chemistry, Analytical
Federico Sala, Carlotta Ficorella, Roberto Osellame, Josef A. Kaes, Rebeca Martinez Vazquez
Summary: Understanding cell migration is crucial for unraveling physiological phenomena and predicting pathologies like cancer metastasis. Microfluidic devices have been used to investigate cellular migration response by engineering mechanical and spatial stimuli. This review discusses the existing technologies, such as soft lithography and femtosecond laser micromachining, used in microfluidic cellular migration assays. It highlights the different geometrical configurations employed and envisions future developments in in vitro migration studies under spatial confinement in microfluidic devices.
Review
Chemistry, Multidisciplinary
Samaneh Zare Harofte, Madjid Soltani, Saeed Siavashy, Kaamran Raahemifar
Summary: Nowadays, artificial intelligence creates numerous opportunities in the life sciences. Integrating AI with microfluidics can significantly impact various fields of biotechnology.
Article
Chemistry, Analytical
Diwakar M. Awate, Cicero C. Pola, Erica Shumaker, Carmen L. Gomes, Jaime J. Juarez
Summary: Despite the limitations of flow cytometers, a 3D-printed imaging platform (3DPIP) has been developed to accurately count particles and conduct fluorescence microscopy efficiently. The 3DPIP utilizes a custom particle counter code and machine vision-based algorithm to identify and count particles, showing comparable accuracy to commercial particle counters. Additionally, the platform can perform static experiments and flow-through experiments effectively, making it a promising tool for use in resource-limited clinical environments.
Article
Biochemical Research Methods
Richard Stebbings, Lili Wang, Janet Sutherland, Martin Kammel, Adolfas K. Gaigalas, Manuela John, Bodo Roemer, Maren Kuhne, Rudolf J. Schneider, Michael Braun, Andrea Engel, Dinesh K. Dikshit, Fatima Abbasi, Gerald E. Marti, Maria Paola Sassi, Laura Revel, Sook-Kyung Kim, Marc-Olivier Baradez, Tamara Lekishvili, Damian Marshall, Liam Whitby, Wang Jing, Volker Ost, Maxim Vonsky, Joerg Neukammer
Article
Biochemical Research Methods
Peter Simon, Marcin Frankowski, Nicole Bock, Joerg Neukammer
Article
Optics
Jonas Gienger, Markus Baer, Jorg Neukammer
Article
Medical Laboratory Technology
Luisa Saraiva, Lili Wang, Martin Kammel, Andreas Kummrow, Eleanor Atkinson, Ji Youn Lee, Burhanettin Yalcinkaya, Muslum Akgoez, Jana Hoeckner, Andreas Ruf, Andrea Engel, Yu-Zhong Zhang, Orla O'Shea, Maria Paola Sassi, Carla Divieto, Tamara Lekishvili, Jonathan Campbell, Yingying Liu, Jing Wang, Richard Stebbings, Adolfas K. Gaigalas, Peter Rigsby, Joerg Neukammer, Sandrine Vessillier
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2019)
Article
Biochemical Research Methods
Jonas Gienger, Hermann Gross, Volker Ost, Markus Baer, Joerg Neukammer
BIOMEDICAL OPTICS EXPRESS
(2019)
Article
Biochemistry & Molecular Biology
Kathrin Smuda, Jonas Gienger, Philipp Hoenicke, Jorg Neukammer
Summary: HbMPs show promise as oxygen therapeutics, but extensive characterization is required for clinical studies. A novel optical method has been developed for quality control and functionality assessment, allowing for simultaneous determination of HbMPs composition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Samreen Falak, Rainer Macdonald, Eloise J. Busby, Denise M. O'Sullivan, Mojca Milavec, Annabell Plauth, Martin Kammel, Heinz Zeichhardt, Hans-Peter Grunert, Andreas Kummrow, Jim F. Huggett
Summary: This paper investigates the potential of reverse transcriptase dPCR (RT-dPCR) in quantifying HIV-1 genomic RNA without calibration. The study suggests that RT-dPCR could be used as a reference measurement procedure to assist in assigning values to HIV-1 reference materials.
Article
Biochemical Research Methods
Mojca Milavec, Jernej Pavsic, Alexandra Bogozalec Kosir, Gerwyn M. Jones, Denise M. O'Sullivan, Alison S. Devonshire, Fran Van Heuverswyn, Maria Karczmarczyk, Jannika Neeb, Annabell Plauth, Philippe Corbisier, Heinz Schimmel, Andreas Kummrow, Joerg Neukammer, Carole A. Foy, Martin Kammel, Hans-Peter Grunert, Heinz Zeichhardt, Jim F. Huggett
Summary: The study evaluated a candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) in multiple schemes, demonstrating high reproducibility and trueness of the method. The dPCR has the potential to act as a calibration independent reference measurement procedure for value assignment of hCMV calibration and support qPCR calibration.
Article
Multidisciplinary Sciences
Jonas Gienger, Kathrin Smuda, Ralph Mueller, Markus Baer, Joerg Neukammer
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Claudia Frank, Christine Brauckmann, Marie Palos, Cristian G. Arsene, Joerg Neukammer, Maria Estela del Castillo Busto, Sabine Zakel, Claudia Swart, Bernd Guettler, Rainer Stosch
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2017)
Article
Optics
Jonas Gienger, Hermann Gross, Joerg Neukammer, Markus Baer
Proceedings Paper
Engineering, Biomedical
M. Kammel, A. Kummrow, M. John, S. Reitz, K. Witt, J. Neukammer
2015 IEEE INTERNATIONAL SYMPOSIUM ON MEDICAL MEASUREMENTS AND APPLICATIONS (MEMEA) PROCEEDINGS
(2015)