期刊
SENSORS
卷 10, 期 6, 页码 5409-5424出版社
MDPI AG
DOI: 10.3390/s100605409
关键词
His-tag; F(ab'); gold electrodes; nanorods
资金
- Polish Ministry of Science and Higher Education [2 P06T 014 29]
- Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland
Immunosensors based on gold electrodes (electrochemical) or gold discs (optical) modified with 1,6-hexanedithiol, gold nanorods and Anti-His (C-term) monoclonal antibody F(ab') fragment are described. The antigen detected by the sensing platform is a recombinant histidine-tagged silk proteinase inhibitor (rSPI2-His(6)). Electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) techniques were used as methods for detection of the antigen. This approach allows to detect the antigen protein in concentration of 10 pg per mL (0.13 pM) of culture medium. The immunosensor shows good reproducibility due to covalent immobilization of F(ab') fragments to gold nanorods layer.
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