期刊
SENSORS
卷 10, 期 7, 页码 6969-6979出版社
MDPI
DOI: 10.3390/s100706969
关键词
autofluorescence; confocal laser scanning microscopy; cyanobacteria; fluorescence imaging; light; microscopy; morphology; phycobiliproteins
资金
- Michigan State University
- National Science Foundation [MCB-0643516]
- Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Sciences, U.S. Department of Energy [DE-FG02-91ER20021]
Fremyella diplosiphon is a freshwater, filamentous cyanobacterium that exhibits light-dependent regulation of photosynthetic pigment accumulation and cellular and filament morphologies in a well-known process known as complementary chromatic adaptation (CCA). One of the techniques used to investigate the molecular bases of distinct aspects of CCA is confocal laser scanning microscopy (CLSM). CLSM capitalizes on the autofluorescent properties of cyanobacterial phycobiliproteins and chlorophyll a. We employed CLSM to perform spectral scanning analyses of F. diplosiphon strains grown under distinct light conditions. We report optimized utilization of CLSM to elucidate the molecular basis of the photoregulation of pigment accumulation and morphological responses in F. diplosiphon.
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