期刊
SENSORS
卷 8, 期 9, 页码 5492-5500出版社
MDPI
DOI: 10.3390/s8095492
关键词
uric acid; uricase; biosensor; polypyrrole; polyaniline
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2O2 at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5x10(-6)-8.5x10(-5) M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 degrees C, respectively. The stability and reproducibility of the enzyme electrode have been also studied.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据