Article
Cell Biology
Bojana Jakic, William J. Olson, Kerstin Siegmund, Victoria Klepsch, Janine Kimpel, Verena Labi, Dietmar Zehn, Gottfried Baier, Natascha Hermann-Kleiter
Summary: The loss of nuclear orphan receptor NR2F6 enhances antigen-specific CD8(+) memory formation and boosts inflammatory cytokine responses in mice after bacterial infection. The enhanced memory formation is intrinsic to CD8(+) T cells and is established early in the antibacterial immune response, mediated by IFN-gamma. Blocking IFN-gamma normalizes the memory precursor cell formation, indicating potential therapeutic benefits of NR2F6 manipulation for memory CD8(+) T cell functionality.
CELL DEATH & DISEASE
(2021)
Article
Immunology
Shi-Lin Qiu, Qi-Xiang Sun, Jian-Peng Zhou, Hai-Juan Tang, Yan-Qiong Chen, Fu-Shou Chen, Tao Feng, Zai-Qing He, Hua-Jiao Qin, Min-Chao Duan
Summary: The research reveals that IL-27 plays a negative regulatory role in chronic inflammation induced by smoking, preventing CD8(+)T cells from producing excessive inflammatory responses. This study provides a new therapeutic strategy for smoking-related COPD/emphysema by targeting the IL-27 pathway.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ke Zhao, Jin-Fang Liu, Ya-Xin Zhu, Xiao-Ming Dong, Rong-Hua Yin, Xian Liu, Hui-Ying Gao, Feng-Jun Xiao, Rui Gao, Qi Wang, Yi-Qun Zhan, Miao Yu, Hui Chen, Hong-Mei Ning, Cai-Bo Zhang, Xiao-Ming Yang, Chang-Yan Li
Summary: Hemgn plays a critical regulatory role in the engraftment and reconstitution of HSPCs by negatively regulating IFN-γ signaling. Hemgn(-/-) HSPCs show defective engraftment during transplantation, which is associated with IFN-γ signaling, and the defect can be rescued by intervention in IFN-γ signaling. Hemgn deficiency sustains nuclear Stat1 tyrosine phosphorylation via suppressing TC45 activity.
Article
Immunology
Miguel A. B. Mercado, Wuying Du, Priyangi A. Malaviarachchi, Jessica Gann, Lin-Xi Li
Summary: The study revealed that innate IFN-gamma is crucial for preventing systemic dissemination of Chlamydia, while IFN-gamma produced by CD4 T cells is largely redundant at the FRT mucosa.
INFECTION AND IMMUNITY
(2021)
Article
Biology
Jakub Tomala, Petra Weberova, Barbora Tomalova, Zuzana Jiraskova Zakostelska, Ladislav Sivak, Jirina Kovarova, Marek Kovar
Summary: The IL-2 and JES6-1 mAb complexes selectively expand CD25(+) cells and T-reg cells, but also increase sensitivity to LPS shock in mice, potentially raising safety concerns for their use in immunotherapy.
Article
Immunology
Huanhuan Wang, Lingzhu Xie, Xuhong Song, Jing Wang, Xinyan Li, Zhike Lin, Ting Su, Bin Liang, Dongyang Huang
Summary: This study found that exogenous purines, through the action of interferon-gamma (IFN-gamma), increase XOR expression, leading to uric acid production. Clinical data showed a positive correlation between serum IFN-gamma concentrations and both purine and uric acid levels, as well as an increased risk of hyperuricemia.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Tarani Kanta Barman, Victor C. Huber, Jesse L. Bonin, Danielle Califano, Sharon L. Salmon, Andrew N. J. McKenzie, Dennis W. Metzger
Summary: This study reveals the interplay between host interferon (IFN)-gamma and viral PB1-F2 virulence protein in regulating the functions of innate lymphoid cells (ILCs) and T cells during influenza virus infection. In the absence of IFN-gamma, lung ILC2s initiate a robust IL-5 response, leading to improved tissue integrity and increased survival. However, the presence of functional PB1-F2 suppresses ILC2 responses and induces a dominant IL-13 CD8 T cell response. This study also demonstrates the importance of IFN-gamma in host resistance against lethal doses of the influenza virus.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Fisheries
Feiyu Fu, Qi Shao, Jie Wang, Jianjian Zhang, Zhaofei Wang, Jingjiao Ma, Yaxian Yan, Yuqiang Cheng, Jianhe Sun
Summary: This study analyzed the cloning and function of BatMAVS, the articulatory protein in bats' immune response against RNA viruses. It was found that BatMAVS plays a crucial role in regulating the interferon response and inhibiting viral replication.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Immunology
Shin Shimoyama, Kazuhito Kawata, Kazuyoshi Ohta, Takeshi Chida, Tetsuro Suzuki, Koichi Tsuneyama, Shinji Shimoda, Nobuhito Kurono, Patrick S. C. Leung, M. Eric Gershwin, Takafumi Suda, Yoshimasa Kobayashi
Summary: The primary treatment for primary biliary cholangitis (PBC), ursodeoxycholic acid (UDCA), works by suppressing the production of IFN-gamma and CX3CL1 to reduce inflammation, ultimately decreasing the chemotactic and adhesive abilities of liver-infiltrating T cells in PBC.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Cell Biology
Xiao-Xi Pan, Kang-Li Yao, Yong-Feng Yang, Qian Ge, Run Zhang, Ping-Jin Gao, Cheng-Chao Ruan, Fang Wu
Summary: Aging-associated chronic inflammation is a key factor contributing to chronic metabolic disorders. Research shows an accumulation of T cells in adipose tissue in old mice, with a cross talk between T cells and preadipocytes contributing to tissue remodeling. Senescent T cells were found to inhibit brown adipocyte differentiation in adipose tissue, with increased production of IFN-gamma identified as a critical mechanism.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Jennie C. Kim, Xian Liu, Karen Fitzgerald, Jason S. Eng, Jessica Orf, Sarah A. O'Brien, Brian Belmontes, Amy-Jo Casbon, Sergey Novitskiy, Kristin Tarbell, Jason DeVoss, Jackson G. Egen
Summary: STING pathway plays context-dependent roles in regulating anti-tumor immunity and response to immunotherapy. In poorly immunogenic tumor models, STING expression in non-tumor cells is crucial for productive anti-tumor immune responses. In immunogenic models, constitutive STING activation in tumor cells can partially bypass the requirement for STING-dependent activity from immune cells.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Cell Biology
Taylor Schmit, Kai Guo, Jitendra Kumar Tripathi, Zhihan Wang, Brett McGregor, Mitch Klomp, Ganesh Ambigapathy, Ramkumar Mathur, Junguk Hur, Michael Pichichero, Jay Kolls, M. Nadeem Khan
Summary: This study identifies IFN-gamma-regulated CCR2(+) monocytes as the main driver of lung damage during IAV infection. IFN-gamma regulates the recruitment and inflammatory phenotype of CCR2(+) monocytes. CD8+ T cells are the main source of IFN-gamma in the infected lungs.
Article
Microbiology
Fang Yang, Yi Yang, Yiwei Chen, Guobao Li, Guoliang Zhang, Lingming Chen, Zhiyi Zhang, Qiongdan Mai, Gucheng Zeng
Summary: This study demonstrates that intestinal dysbiosis increases susceptibility to tuberculosis (TB) and leads to increased expression of miR-21 in the lungs. Systemic antagonism of miR-21 enhances IFN-gamma production and confers immune protection against TB. The findings suggest that miR-21a-3p can specifically target IFN-gamma mRNA, revealing regulatory pathways linking intestinal dysbiosis-induced susceptibility to TB.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Emi Hashizaki, Miwa Sasai, Daisuke Okuzaki, Tsubasa Nishi, Takashi Kobayashi, Shiroh Iwanaga, Masahiro Yamamoto
Summary: The transcription factor IWS1 in Toxoplasma gondii indirectly regulates ROP18 mRNA expression, thus determining the parasite's fitness in IFN-gamma-activated host cells and mice. Loss of IWS1 leads to decreased ROP18 mRNA expression and significantly reduced virulence due to increased activity of IFN-gamma-dependent immune responses. The findings suggest that Toxoplasma prepares the critical virulence factor ROP18 via an IWS1-dependent system to survive in the host.
Article
Cell Biology
Porntida Kobpornchai, Ellen-Alana Tiffney, Poom Adisakwattana, Robin J. Flynn
Summary: This study investigated the impact of TsCstN derived from Trichinella spiralis L1 stage on macrophage-T cell interaction and immune responses. The results showed that TsCstN negatively regulated IFN-gamma production in macrophages, but not IL-17A, as well as affected IL-12 levels and the autonomous response in myotubule cells. This work reveals a potential pathway for L1 larvae to evade immune responses and establish infection.
CELLULAR IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Elena Lorente, Antonio J. Martin-Galiano, Dganit Melamed Kadosh, Alejandro Barriga, Juan Garcia-Arriaza, Carmen Mir, Mariano Esteban, Arie Admon, Daniel Lopez
Summary: This study utilized high-throughput mass spectrometry analysis to identify a large number of cell peptides bound to HLA-DR and -DP class II molecules, finding that these cell proteins were more acidic, abundant, and highly connected, but less hydrophilic compared to non-parental proteins.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Immunology
Patricia Perez, Adrian Lazaro-Frias, Carmen Zamora, Pedro J. Sanchez-Cordon, David Astorgano, Joanna Luczkowiak, Rafael Delgado, Jose M. Casasnovas, Mariano Esteban, Juan Garcia-Arriaza
Summary: Researchers developed an optimized COVID-19 vaccine candidate using a modified vaccinia virus Ankara vector, which expressed a full-length prefusion-stabilized SARS-CoV-2 spike protein. The vaccine showed enhanced immunogenicity and efficacy against SARS-CoV-2 in animal models, making it a promising candidate for clinical trials.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Adrian Lazaro-Frias, Patricia Perez, Carmen Zamora, Pedro J. Sanchez-Cordon, Maria Guzman, Joanna Luczkowiak, Rafael Delgado, Jose M. Casasnovas, Mariano Esteban, Juan Garcia-Arriaza
Summary: The MVA-CoV2-S vaccine candidate protected transgenic mice from a lethal dose of SARS-CoV-2 through two doses, inducing high levels of antibodies and providing long-term memory immune responses. The vaccine showed efficacy in preventing virus replication, reducing lung pathology, and inducing protective antibody responses against the parental virus and variants of concern.
Article
Rheumatology
Max Loehning, Ping Shen, Maria Dzamukova, Nayar Duran-Hernandez, Jay Roodselaar, Anja E. Hauser, Alexander Fiedler, Raluca A. Niesner, Timo Gaber, Frank Buttgereit
Summary: Rheumatoid arthritis and osteoarthritis are chronic diseases affecting joints and bones. Chronic inflammation, mechanical stimuli, and blood vessels play important roles in bone growth and tissue regeneration. Research on molecular, cellular, and tissue-level changes in chronic inflammatory diseases is crucial for developing better prevention and treatment strategies.
ZEITSCHRIFT FUR RHEUMATOLOGIE
(2022)
Article
Multidisciplinary Sciences
Maria Dzamukova, Tobias M. Brunner, Jadwiga Miotla-Zarebska, Frederik Heinrich, Laura Brylka, Mir-Farzin Mashreghi, Anjali Kusumbe, Ralf Kuehn, Thorsten Schinke, Tonia L. Vincent, Max Loehning
Summary: The study reveals that mechanical forces trigger the secretion of dentin matrix protein 1 from osteoblasts, leading to the transformation of bone growth-promoting blood vessels into a quiescent subtype, thus limiting bone growth at the end of adolescence.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Yonghai Li, Haiyao Gao, Tobias M. Brunner, Xiaoxi Hu, Yushan Yan, Yanli Liu, Liang Qiao, Peihua Wu, Meng Li, Qing Liu, Fen Yang, Juntang Lin, Max Loehning, Ping Shen
Summary: This study evaluated the therapeutic potential of allogeneic MSCs isolated from menstrual blood in an animal model of experimental autoimmune encephalomyelitis (EAE), showing that MB-MSCs significantly reduced the disease severity of EAE and suppressed Th1 and Th17 cell responses, suggesting their potential as ready-to-use allo-MSCs in MS-related inflammation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Immunology
Van Duc Dang, Elodie Mohr, Franziska Szelinski, Tuan Anh Le, Jacob Ritter, Timo Hinnenthal, Ana-Luisa Stefanski, Eva Schrezenmeier, Soeren Ocvirk, Christian Hipfl, Sebastian Hardt, Qingyu Cheng, Falk Hiepe, Max Loehning, Thomas Doerner, Andreia C. Lino
Summary: A large-scale protein screening identified twelve new molecules specifically expressed by murine ASCs, with stable expression of CD39, CD81, CD130, and CD326 offering improved resolution for ASC identification. Further analysis revealed a subpopulation of ASC in autoimmunity expressing high levels of CD39 and CD326.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Philipp Burt, Michael Peine, Caroline Peine, Zuzanna Borek, Sebastian Serve, Michael Flossdorf, Ahmed N. Hegazy, Thomas Hoefer, Max Loehning, Kevin Thurley
Summary: Selective differentiation of CD4+ T helper (Th) cells into specialized subsets is crucial for appropriate immune responses. While canonical Th-cell lineages are well understood, the transcriptional architecture of hybrid phenotypes remains unclear. This study explored the transcriptome of Th-cell differentiation, revealing an early bifurcation point in gene expression programs. Hybrid Th1/2 cells showed mixed effects from Th1 and Th2 cells for a minority of genes, while others followed a Th1 and Th2 cell-independent transcriptional program. These findings emphasize the importance of longitudinal data for characterizing cellular phenotypes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Neurosciences
Javier Villadiego, Juan Garcia-Arriaza, Reposo Ramirez-Lorca, Roberto Garcia-Swinburn, Daniel Cabello-Rivera, Alicia E. Rosales-Nieves, Maria I. Alvarez-Vergara, Fernando Cala-Fernandez, Ernesto Garcia-Roldan, Juan L. Lopez-Ogayar, Carmen Zamora, David Astorgano, Guillermo Albericio, Patricia Perez, Ana M. Munoz-Cabello, Alberto Pascual, Mariano Esteban, Jose Lopez-Barneo, Juan Jose Toledo-Aral
Summary: In this study using a transgenic mouse model, it was found that SARS-CoV-2 primarily replicates in neurons in the brain, causing neuronal loss, glial activation, and vascular damage. However, vaccination with the MVA-CoV2-S vaccine provided full protection against cerebral infection and associated damage, even after reinfection.
NATURE NEUROSCIENCE
(2023)
Article
Immunology
Belen Aparicio, Marta Ruiz, Noelia Casares, Leyre Silva, Josune Egea, Patricia Perez, Guillermo Albericio, Mariano Esteban, Juan Garcia-Arriaza, Juan J. Lasarte, Pablo Sarobe
Summary: Current vaccines based on the original Wuhan sequence induce antibodies that can cross-recognize new variants to different extents. However, breakthrough infections caused by the Omicron variant highlight the need for better immunity. A study compared the immune response induced by a Wuhan-based peptide vaccine with one based on the Omicron sequence, and found that the Wuhan-based peptide vaccine showed strong cross-recognition of Omicron, suggesting its potential for inclusion in future vaccines targeting the Omicron variant.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ping Shen, Peihua Wu, Tazio Maleitzke, Marie-Jacqueline Reisener, Gitta A. Heinz, Frederik Heinrich, Pawel Durek, Clemens Gwinner, Tobias Winkler, Matthias Pumberger, Carsten Perka, Mir-Farzin Mashreghi, Max Loehning
Summary: This study optimized the isolation procedure of human chondrocytes from articular cartilage to preserve the in vivo transcriptome. By using appropriate concentrations of collagenase and digestion time, the maximum release and preservation of chondrocyte transcriptome were achieved.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Immunology
Lorena Usero, Lorna Leal, Carmen Elena Gomez, Laia Miralles, Elena Aurrecoechea, Ignasi Esteban, Berta Torres, Alexy Inciarte, Beatriz Perdiguero, Mariano Esteban, Felipe Garcia, Montserrat Plana
Summary: Researchers tested a novel HIV therapeutic vaccine using unmodified mRNA (TMEP-B) and mRNA modified by 1-methyl-3'-pseudouridylyl (TMEP-Bmod) that expressed sequences from Gag, Pol, and Nef proteins in transfected monocyte-derived dendritic cells (MDDCs) obtained from HIV-infected patients. In vitro experiments were conducted to test the mRNAs alone and in combination with immunomodulators to enhance HIV-specific cellular immune responses. The combination of mRNAs with immunomodulators increased HIV-specific T-cell responses and the secretion of mediators involved in viral control, suggesting that these mRNA vaccines could potentially achieve a functional cure for HIV patients when combined with Vesatolimod and/or Nivolumab.
Article
Immunology
Anna-Friederike Marx, Sandra M. Kallert, Tobias M. Brunner, Jose A. Villegas, Florian Geier, Jonas Fixemer, Tiago Abreu-Mota, Peter Reuther, Weldy V. Bonilla, Jelizaveta Fadejeva, Mario Kreutzfeldt, Ingrid Wagner, Patricia Aparicio-Domingo, Leo Scarpellino, Melanie Charmoy, Daniel T. Utzschneider, Claudia Hagedorn, Min Lu, Karen Cornille, Karsten Stauffer, Florian Kreppel, Doron Merkler, Dietmar Zehn, Werner Held, Sanjiv A. Luther, Max Loehning, Daniel D. Pinschewer
Summary: This study shows that T cell factor 1 (Tcf-1) expressing CD8+ T cells, known as stem-like CD8+ T cells (CD8+SL), play a crucial role in immune defense against chronic viral infection and cancer. It identifies interleukin-33 (IL-33) as a pivotal factor for the expansion and functioning of CD8+SL and virus control.
Article
Multidisciplinary Sciences
Angelique Wolter, Christian H. Bucher, Sebastian Kurmies, Viktoria Schreiner, Frank Konietschke, Katharina Hohlbaum, Robert Klopfleisch, Max Loehning, Christa Thoene-Reineke, Frank Buttgereit, Joerg Huwyler, Paulin Jirkof, Anna E. Rapp, Annemarie Lang
Summary: In animal experiments, adequate pain management is necessary and should last for the expected pain period without repeated application. The current depot formulations of Buprenorphine have limited duration of action, but a new sustained-release formulation (BUP-Depot) has been developed as a potential alternative in Europe. It provides effective analgesia for 72 hours, similar to Tramadol in the drinking water.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Holly Bachus, Erin McLaughlin, Crystal Lewis, Amber M. Papillion, Etty N. Benveniste, Dave Durell Hill, Alexander F. Rosenberg, Andre Ballesteros-Tato, Beatriz Leon
Summary: Defective IL-6 signaling is linked to Th2 bias and elevated IgE levels. The study reveals that IL-6 signaling in allergen-specific T cells is necessary to prevent Th2 cell differentiation, IgE response, and allergic inflammation. IL-6 inhibits IL-2 signaling through upregulation of SOCS3, thus preventing Th2 priming.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)