期刊
SCIENTIFIC REPORTS
卷 5, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep08324
关键词
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资金
- Fondation pour la Recherche Medicale
- Institut National de la Sante et de la Recherche Medicale
- Centre National pour la Recherche Scientifique
- Strasbourg University
- Association pour la Recherche sur le Cancer
- Agence Nationale pour la Recherche (CHROMACT)
- Labex INRT
- French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INSB-05-01]
- INSTRUCT, European Strategy Forum on Research Infrastructures (ESFRI)
Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of entire cells with probe localization accuracy in the 10 nm range.
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