Article
Medicine, Research & Experimental
Alec A. Schmaier, Papa F. Anderson, Siyu M. Chen, Emale El-Darzi, Ivan Aivasovsky, Milan P. Kaushik, Kelsey D. Sack, H. Criss Hartzell, Samir M. Parikh, Robert Flaumenhaft, Sol Schulman
Summary: The study found that endothelial cells can switch from an anticoagulant surface to a procoagulant surface under pathogenic stimuli, promoting thrombotic cardiovascular disease. Two TMEM16 family members, TMEM16F and TMEM16E, were identified as crucial for the procoagulant activity of endothelial cells. In addition, the TMEM16 inhibitor benzbromarone was found to prevent endothelial cell procoagulant activity and thrombus formation.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Jens Van Bael, Aline Vandenbulcke, Abdelhakim Ahmed-Belkacem, Jean-Francois Guichou, Jean-Michel Pawlotsky, Jelle Samyn, Arjan D. Barendrecht, Coen Maas, Simon F. De Meyer, Karen Vanhoorelbeke, Claudia Tersteeg
Summary: Inhibiting CypD activity with novel non-immunosuppressive cyclophilin inhibitors (SMCypIs) can effectively reduce procoagulant platelet formation and provide a promising strategy for limiting thrombosis without affecting normal platelet function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Rainer Kaiser, Raphael Escaig, Jan Kranich, Marie-Louise Hoffknecht, Afra Anjum, Vivien Polewka, Magdalena Mader, Wenbo Hu, Larissa Belz, Christoph Gold, Anna Titova, Michael Lorenz, Kami Pekayvaz, Stefan Kaab, Florian Gaertner, Konstantin Stark, Thomas Brocker, Steffen Massberg, Leo Nicolai
Summary: Impairment of vascular integrity is a characteristic of inflammatory diseases. This study demonstrates the essential role of procoagulant platelets in preventing inflammatory bleeding by promoting coagulation cascade activation. The presence of exposed collagen triggers platelet arrest and subsequent procoagulant activation. The findings highlight the importance of procoagulant platelets in maintaining vascular integrity during inflammation.
Article
Hematology
Christopher Carman, Dessislava N. Nikova, Yumiko Sakurai, Jialan Shi, Valerie A. Novakovic, Jan T. Rasmussen, Wilbur A. Lam, Gary E. Gilbert
Summary: Prior reports have indicated that convex membrane curvature of phosphatidylserine (PS)-containing vesicles enhances the formation of binding sites for factor Va and lactadherin, but the relationship between convex curvature and protein localization on cells remains unknown. In this study, a membrane topology model was developed to explore this relationship further. Results show that the ridges of membranes with maximal curvature selectively bound lactadherin and factor Va, supporting the assembly of prothrombinase complexes.
Article
Physiology
Agata Golaszewska, Tomasz Misztal, Natalia Marcinczyk, Ewa Chabielska, Tomasz Rusak
Summary: The study shows that adrenaline can enhance platelet activation and thrombus formation in the presence of subthreshold collagen, leading to increased platelet aggregation and formation of denser platelet-fibrin clots. These effects are associated with a decrease in fibrinolysis rate, which can be reduced by commonly used antiplatelet drugs.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Hematology
Yalong Gao, Xiaotian Li, Yafei Qin, Jianlong Men, Jing Ren, Xiaochun Li, Chunlei Xu, Qifeng Li, Ying Li, Weiyun Cui, Shu Zhang, Lei Li, Yaohua Li, Jianning Zhang, Li Liu
Summary: The study found that microparticles (MPs) have a procoagulant effect that contributes to hypercoagulability-induced thrombosis. The MPs-ACT assay was used to determine the procoagulant activity of MPs by measuring changes in plasma viscoelasticity and the time to peak viscoelastic changes. The results showed a linear relationship between MPs-ACT and MPs concentration, and the assay sensitively detected hypercoagulable samples from patients with pre-eclampsia, hip fractures, and lung tumors.
CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS
(2023)
Article
Pharmacology & Pharmacy
Eriko Nakatani, Yasuo Naito, Kenichi Ishibashi, Naoki Ohkura, Gen-Ichi Atsumi
Summary: Obesity is associated with the risk of venous thromboembolism, and adipocyte-derived extracellular vesicles (ADEVs) can enhance coagulant activity. ADEVs contain tissue factors and phosphatidylserine involved in clot formation, and this effect is further enhanced by proinflammatory cytokines.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Biophysics
Kenji Miyazawa, Aaron L. Fogelson, Karin Leiderman
Summary: Blood coagulation is a self-repair process regulated by activated platelet surfaces, clotting factors, and inhibitors. Recently, a new role for TFPI in inhibiting thrombin generation on activated platelet surfaces has been discovered, which is independent of TF:VIIa. This study found that this TF-independent inhibitory mechanism is more effective than the TF-dependent one.
BIOPHYSICAL JOURNAL
(2023)
Article
Immunology
Emilly Caroline dos Santos Moraes, Remy Martins-Goncalves, Luana Rocha da Silva, Samuel Coelho Mandacaru, Reynaldo Magalhaes Melo, Isaclaudia Azevedo-Quintanilha, Jonas Perales, Fernando A. Bozza, Thiago Moreno Lopes Souza, Hugo Caire Castro-Faria-Neto, Eugenio D. Hottz, Patricia T. Bozza, Monique R. O. Trugilho
Summary: This study identified significant changes in the proteome of extracellular vesicles (EVs) from severe COVID-19 patients using a label-free shotgun proteomic approach. Flow cytometry experiments showed an increase in total circulating EVs with procoagulant activity. Differentially expressed proteins in the disease groups were associated with complement and coagulation cascades, platelet degranulation, and acute inflammatory response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Critical Care Medicine
Ingrid Nygren Rognes, Marit Hellum, William Ottestad, Kristi Gronvold Bache, Torsten Eken, Carola Elisabeth Henriksson
Summary: This study aimed to explore the changes in EV-associated procoagulant activity in trauma patients after injury, finding that EV-associated procoagulant activity peaked in the first three hours after injury and then gradually decreased to levels close to healthy volunteers. Increased EV-associated procoagulant activity at admission was associated with various trauma severity indicators and outcomes.
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2021)