Article
Cell & Tissue Engineering
Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S. Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R. Iyer, Thomas E. Lloyd, Richard M. Lovering, Su Bin Lim, Peter Andersen, Kathryn R. Wagner, Gabsang Lee, Chulan Kwon
Summary: Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation can contribute to muscle fiber regeneration in mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
Article
Immunology
Hoa Le Mai, Nicolas Degauque, Marine Lorent, Marie Rimbert, Karine Renaudin, Richard Danger, Clarisse Kerleau, Gaelle Tilly, Anais Vivet, Sabine Le Bot, Florent Delbos, Alexandre Walencik, Magali Giral, Sophie Brouard
Summary: This study found that kidney allograft rejection is associated with an imbalance between immune cells with effector/memory functions and those with regulatory properties, manifested as a decrease in the percentage of circulating B cells and an increase in the ratio of CD28-CD8+ T cells to Tregs.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Maite Alvarez, Antonio Pierini, Federico Simonetta, Jeanette Baker, Kristina Maas-Bauer, Toshihito Hirai, Robert S. Negrin
Summary: Allogeneic hematopoietic cell transplantation is an effective treatment option for hematological malignancies, but often results in severe toxicities. Less aggressive alternatives like NMAC and RIC have limitations including increased cancer relapse and limited persistence of donor chimerism. Strategies for accelerated and more durable donor engraftment are still needed.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Hirohito Ishigaki, Van Loi Pham, Jun Terai, Takako Sasamura, Cong Thanh Nguyen, Hideaki Ishida, Junko Okahara, Shin Kaneko, Takashi Shiina, Misako Nakayama, Yasushi Itoh, Kazumasa Ogasawara
Summary: This study found that transplantation of iPSCs in MHC-matched immune-competent cynomolgus macaques did not result in tumor formation, suggesting that iPSCs may not have tumorigenicity in MHC-matched allogeneic transplantation for clinical application.
CELL TRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
Yandan Wu, Zhenzhen Zhang, Shuangshuang Wu, Zhaolong Chen, Yue Pu
Summary: Researchers have developed a highly sensitive assay to detect undifferentiated cells in islet cells derived from human chemically induced pluripotent stem cells (hCiPSCs). They identified biomarkers using RNA-seq data and confirmed them through RT-qPCR and ddPCR. This study provides a method to ensure the safety and quality of hCiPSC-derived islet cells for clinical use.
SCIENTIFIC REPORTS
(2023)
Review
Cell Biology
Chenxin Wang, Jingjing Liu, Wei Li
Summary: In recent years, immune cell-based therapies using primary T cells and NK cells armed with chimeric antigen receptors have shown great success in treating refractory malignancies. However, the autologous origin of these cells limits their accessibility to patients due to high cost, product quality variations, and treatment delays. Therefore, the development of universal immune cell products from pluripotent stem cells (PSCs) is necessary to overcome these challenges and provide a large supply of engineered immune cells. This review discusses the advances in PSC-derived immune cell differentiation approaches and their potential in treating various diseases.
CELL PROLIFERATION
(2023)
Article
Immunology
Grace Lee, Robert Schauner, Juanita Burke, Jade Borocz, Smitha Vasana, Lukasz Sobieraj, Maria Giraudo, Zachary Jackson, Qasim Ansari, Maria Navas, Hany Sakr, David Wald
Summary: SARS-CoV-2 has caused a global pandemic with significant morbidity and mortality. This study identifies specific clusters of NK cells associated with COVID-19 and characterizes the phenotype of COVID-19 positive NK cells. Blocking NKG2A and KIR2DL1 receptors enhances the ability of NK cells from COVID-19 positive patients to kill SARS-CoV-2 infected cells, suggesting a potential therapeutic approach.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Toru Kondo
Summary: The study demonstrates that the DHODH inhibitor BRQ induces differentiation in mouse PSCs, preventing teratoma formation, while showing low toxicity to normal cells. This suggests that DHODH inhibitors like BRQ could be crucial for successful PSC-based therapy.
Editorial Material
Cell & Tissue Engineering
Elyad Lezmi, Nissim Benvenisty
Summary: Assessing and enhancing the safety of human pluripotent stem cell (hPSC)-based treatments are crucial due to the tumorigenic potential of hPSCs. Understanding the molecular features and genetic aberrations associated with tumorigenicity is important for developing precautionary and safety measures in hPSC therapy.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Ahmed Elhussieny, Ken'ichiro Nogami, Fusako Sakai-Takemura, Yusuke Maruyama, Natsumi Takemura, Wael Talaat Soliman, Shin'ichi Takeda, Yuko Miyagoe-Suzuki
Summary: The study demonstrated that induced MSC-like cells (iMSCs) promoted the proliferation and differentiation of human myogenic cells and enhanced their engraftment in a DMD model. iMSCs secreted cytokines that facilitated the migration and engraftment of human myogenic cells, showing potential for improving muscle regeneration in dystrophic conditions.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Urology & Nephrology
Alice Koenig, Sarah Mezaache, Jasper Callemeyn, Thomas Barba, Virginie Mathias, Antoine Sicard, Beatrice Charreau, Maud Rabeyrin, Frederique Dijoud, Cecile Picard, Vannary Meas-Yedid, Jean-Christophe Olivo-Marin, Emmanuel Morelon, Maarten Naesens, Valerie Dubois, Olivier Thaunat
Summary: The study found that the presence of missing self may exacerbate DSA-dependent NK cell activation, leading to worsening chronic antibody-mediated rejection. Detection of missing self in allograft endothelial cells can help identify patients at higher risk for kidney transplant failure.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Immunology
Li Yanli, Ivan Diaz, Gerard Martin-Valls, Niklas Beyersdorf, Enric Mateu
Summary: This study investigated the role of cytotoxic lymphocytes in preventing transplacental infection of PRRSV-1 in vaccinated sows, and found that the health status of the piglets was associated with the immune response of these cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kenichi Ishiyama, Janice Arakawa-Hoyt, Oscar A. Aguilar, Izabella Damm, Parhom Towfighi, Tara Sigdel, Stanley Tamaki, Joel Babdor, Matthew H. Spitzer, Elaine F. Reed, Minnie M. Sarwal, Lewis L. Lanier
Summary: The study found that memory-like natural killer (NK) cells and NKG2C-expressing CD8(+) T cells increase in renal transplant patients during cytomegalovirus (CMV) infection, playing a crucial role in combating the CMV virus. The dynamic changes of these cells during CMV viremia may be associated with controlling the virus and preventing graft rejection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Baptiste Lamarthee, Jasper Callemeyn, Yannick Van Herck, Asier Antoranz, Dany Anglicheau, Patrick Boada, Jan Ulrich Becker, Tim Debyser, Frederik De Smet, Katrien De Vusser, Maeva Eloudzeri, Amelie Franken, Wilfried Gwinner, Priyanka Koshy, Dirk Kuypers, Diether Lambrechts, Pierre Marquet, Virginie Mathias, Marion Rabant, Minnie M. Sarwal, Aleksandar Senev, Tara K. Sigdel, Ben Sprangers, Olivier Thaunat, Claire Tinel, Thomas Van Brussel, Amaryllis Van Craenenbroeck, Elisabet Van Loon, Thibaut Vaulet, Francesca Bosisio, Maarten Naesens
Summary: Although kidney allograft failure is classified as either T cell- or antibody-mediated, this distinction is not always clear in all patients, emphasizing the need for better characterization of allograft tissue. The authors used single-cell RNA sequencing and multiplex imaging to analyze the transcriptomes and spatial profiles of allograft tissue from patients with different degrees of rejection severity. The study identified specific cell types and gene expression signatures associated with rejection, highlighting the involvement of innate immune cells in allograft rejection and suggesting potential therapeutic targets to improve graft longevity.
NATURE COMMUNICATIONS
(2023)
Article
Urology & Nephrology
Kevin Louis, Paul Fadakar, Camila Macedo, Masaki Yamada, Michelle Lucas, Xinyan Gu, Adriana Zeevi, Parmjeet Randhawa, Carmen Lefaucheur, Diana Metes
Summary: This study used high dimensional flow cytometry to analyze the status of regulatory T cells and transitional B cells in kidney transplant recipients. It found that in patients with antibody-mediated rejection (ABMR), both cell types were significantly reduced in numbers, which correlated with inflammatory antibody responses, microvascular inflammation, and kidney allograft loss.
KIDNEY INTERNATIONAL
(2022)
Meeting Abstract
Oncology
Ralf Dressel
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2018)
Article
Otorhinolaryngology
Jennifer L. Spiegel, Mario Hambrecht, Vera Kohlbauer, Frank Haubner, Friedrich Ihler, Martin Canis, Arndt F. Schilling, Kai O. Boeker, Ralf Dressel, Katrin Streckfuss-Boemeke, Mark Jakob
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2019)
Article
Cell & Tissue Engineering
Daniela Huebscher, Sabine Rebs, Luis Haupt, Thomas Borchert, Celina Isabell Guessoum, Franziska Treu, Steffen Koehne, Andreas Maus, Mario Hambrecht, Samuel Sossalla, Ralf Dressel, Angela Uy, Mark Jakob, Gerd Hasenfuss, Katrin Streckfuss-Boemeke
STEM CELLS INTERNATIONAL
(2019)
Review
Immunology
Elena Garelli, Achim Rittmeyer, Paul Martin Putora, Markus Glatzer, Ralf Dressel, Stefan Andreas
Article
Biochemistry & Molecular Biology
Hu Li, Tina K. Kaiser, Marina Borschiwer, Hanibal Bohnenberger, Sybille D. Reichardt, Fred Luehder, Lutz Walter, Ralf Dressel, Sebastiaan H. Meijsing, Holger M. Reichardt
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2019)
Article
Cell & Tissue Engineering
Daniela Huebscher, Sabine Rebs, Wiebke Maurer, Jelena R. Ghadri, Ralf Dressel, Christian Templin, Katrin Streckfuss-Boemeke
STEM CELL RESEARCH
(2020)
Article
Cell Biology
Michael Stauske, Ignacio Rodriguez Polo, Wadim Haas, Debbra Yasemin Knorr, Thomas Borchert, Katrin Streckfuss-Boemeke, Ralf Dressel, Iris Bartels, Malte Tiburcy, Wolfram-Hubertus Zimmermann, Ruediger Behr
Review
Immunology
Jukka Partanen, Kati Hyvarinen, Heike Bickeboller, Katarzyna Bogunia-Kubik, Rachel E. Crossland, Milena Ivanova, Francesca Perutelli, Ralf Dressel
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Cell Biology
Mark Jakob, Mario Hambrecht, Jennifer L. Spiegel, Julia Kitz, Martin Canis, Ralf Dressel, Katrin Streckfuss-Bomeke
Summary: A multimodal therapeutic approach involving radiotherapy is essential for treating head and neck squamous cell carcinoma, but its use is limited due to the risk of damaging surrounding healthy tissue. Mesenchymal stem cells show promising therapeutic potential in cell-based tissue engineering and regenerative medicine due to their immunomodulatory properties. Generating induced pluripotent stem cell-derived mesenchymal stem cells in an autologous manner could be a novel personalized treatment option in regenerative medicine.
Article
Multidisciplinary Sciences
Ignacio Rodriguez-Polo, Sophie Missbach, Stoyan Petkov, Felix Mattern, Anna Maierhofer, Iga Grzadzielewska, Yuliia Tereshchenko, Daniel Urrutia-Cabrera, Thomas Haaf, Ralf Dressel, Iris Bartels, Ruediger Behr
Summary: NHPs are valuable animal models for studying clinically relevant mutations due to their close relationship to humans, but the toolbox for genetic modification of NHPs is less developed compared to other species. PSCs share key molecular signatures with the early embryo and are important for validating genome editing approaches. The piggyBac transposon system has been utilized for various purposes in NHP stem cell technology and genome editing, showing advancements in reprogramming, culture, CRISPR/Cas-based genome editing, and establishment of gene-edited monoclonal NHP-iPSC lines.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Luis Peter Haupt, Sabine Rebs, Wiebke Maurer, Daniela Huebscher, Malte Tiburcy, Steffen Pabel, Andreas Maus, Steffen Koehne, Rewati Tappu, Jan Haas, Yun Li, Andre Sasse, Celio C. X. Santos, Ralf Dressel, Leszek Wojnowski, Gertrude Bunt, Wiebke Moebius, Ajay M. Shah, Benjamin Meder, Bernd Wollnik, Samuel Sossalla, Gerd Hasenfuss, Katrin Streckfuss-Boemeke
Summary: This study developed a patient-specific stem cell model to investigate DOX-induced cardiac dysfunction. The results showed that DOX-induced stress led to arrhythmogenic events and contractile dysfunction, ultimately resulting in heart failure.
BASIC RESEARCH IN CARDIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hannes Steinkellner, Prakasha Kempaiah, Alexander Beribisky, Sandra Pferschy, Julia Etzler, Anna Huber, Victoria Sarne, Winfried Neuhaus, Mario Kuttke, Jan Bauer, Jayamuruga P. Arunachalam, John Christodoulou, Ralf Dressel, Alexander Mildner, Marco Prinz, Franco Laccone
Summary: This study demonstrates the therapeutic potential of recombinant TAT-MeCP2 in treating Rett syndrome. The findings show that TAT-MeCP2 can reverse the pathological changes of the disease and extend the lifespan of mice. This research lays the foundation for the development of a new therapy for Rett syndrome.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Carina Matos, Andreas Mamilos, Pranali N. Shah, Elisabeth Meedt, Daniela Weber, Saroj Ghimire, Andreas Hiergeist, Andre Gessner, Anne Dickinson, Ralf Dressel, Lutz Walter, Klaus Stark, Iris M. Heid, Hendrik Poeck, Matthias Edinger, Daniel Wolff, Wolfgang Herr, Ernst Holler, Marina Kreutz, Sakhila Ghimire
Summary: This study demonstrates the downregulation of vitamin D receptor (VDR) gene expression in patients with severe acute gastrointestinal graft versus host disease (GI-GvHD) after allogeneic haematopoietic stem cell transplantation (HSCT). The reduced VDR expression is associated with higher treatment-related mortality and may play an essential role in the pathogenesis of gut GvHD and the prognosis of patients undergoing HSCT.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Mohammad Zahidul Hasan, Charlotte Hoeltermann, Beatrix Petersen, Annette Schrod, Kerstin Maetz-Rensing, Artur Kaul, Gabriela Salinas, Ralf Dressel, Lutz Walter
Summary: This study used newly established monoclonal antibodies to detect the expression of NKG2C receptors on primary NK cells in rhesus macaques. The results showed that NKG2C receptor expression was higher in CMV-positive macaques. Furthermore, these NKG2C-positive NK cells had specific molecular features and exhibited high expression of IFNG, indicating their association with CMV.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Stoyan Petkov, Ralf Dressel, Ignacio Rodriguez-Polo, Rudiger Behr
